LOCUS       Exported                5704 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Previously pFdR4Hn
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(1..188)
                     /label=SV40\polyA\signal
     misc_feature    complement(192..254)
                     /label=MCS 2
     promoter        255..845
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          960..1092
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1136..1155
                     /label=T7 promoter
     misc_feature    1170..2060
                     /label=HaloTag
                     /note="Amino Acid sequence is identical to other HT7 
                     versions, but different DNA sequence."
     misc_feature    2061..2099
                     /label=HT7 Linker N terminal
     misc_feature    2100..2107
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2109..2633
                     /label=BCL2A1
                     /note="BCL2A1"
     misc_feature    2634..2641
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    2737..2958
                     /label=Sv40 late polyadenylation signal
     misc_feature    complement(2982..3015)
                     /label=Cre site
                     /note="Cre site"
     promoter        3024..3090
                     /label=EM7 bacterial promoter
     CDS             3104..3898
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     rep_origin      4053..4089
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(4771..4818)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(4842..5240)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(5265..5683)
                     /label=SV40 promoter
                     /note="SV40 promoter"
ORIGIN
        1 cggcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
       61 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      121 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      181 gattatgatc cgcggccgca atacgtcgac gttatcagct gacttcgtac gagagcctag
      241 gattatggcg cgccactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      481 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      841 agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg
      901 cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg
      961 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
     1021 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
     1081 ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtattaata
     1141 cgactcacta tagggctagc aaagccacca tggcagaaat cggtactggc tttccattcg
     1201 acccccatta tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg
     1261 atggcacccc tgtgctgttc ctgcacggta acccgacctc ctcctacgtg tggcgcaaca
     1321 tcatcccgca tgttgcaccg acccatcgct gcattgctcc agacctgatc ggtatgggca
     1381 aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc atggatgcct
     1441 tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg ggctccgctc
     1501 tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca tttatggagt
     1561 tcatccgccc tatcccgacc tgggacgaat ggccagaatt tgcccgcgag accttccagg
     1621 ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg
     1681 gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt cgagatggac cattaccgcg
     1741 agccgttcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac gagctgccaa
     1801 tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga atacatggac tggctgcacc
     1861 agtcccctgt cccgaagctg ctgttctggg gcaccccagg cgttctgatc ccaccggccg
     1921 aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc ggcccgggtc
     1981 tgaatctgct gcaagaagac aacccggacc tgatcggcag cgagatcgcg cgctggctgt
     2041 cgacgctcga gatttccggc gagccaacca ctgaggatct gtactttcag agcgataacg
     2101 cgatcgccat gacagactgt gaatttggat atatttacag gctggctcag gactatctgc
     2161 agtgcgtcct acagatacca caacctggat caggtccaag caaaacgtcc agagtgctac
     2221 aaaatgttgc gttctcagtc caaaaagaag tggaaaagaa tctgaagtca tgcttggaca
     2281 atgttaatgt tgtgtccgta gacactgcca gaacactatt caaccaagtg atggaaaagg
     2341 agtttgaaga cggcatcatt aactggggaa gaattgtaac catatttgca tttgaaggta
     2401 ttctcatcaa gaaacttcta cgacagcaaa ttgccccgga tgtggatacc tataaggaga
     2461 tttcatattt tgttgcggag ttcataatga ataacacagg agaatggata aggcaaaacg
     2521 gaggctggga aaatggcttt gtaaagaagt ttgaacctaa atctggctgg atgacttttc
     2581 tagaagttac aggaaagatc tgtgaaatgc tatctctcct gaagcaatac tgtgtttaaa
     2641 cgaattcggg ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagctgat
     2701 ttggctgctg ccaccgctga gcaaggccgc ttcgagcaga catgataaga tacattgatg
     2761 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2821 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     2881 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttta agcaagtaaa
     2941 acctctacaa atgtggtaaa atcttaatta agaattctaa tataacttcg tatagcatac
     3001 attatacgaa gttatggatc cactgttgac aattaatcat cggcatagta tatcggcata
     3061 gtataatacg acaaggtgag gaactaaacc caggaggcag atcatgattg aacaagatgg
     3121 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     3181 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     3241 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     3301 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     3361 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     3421 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3481 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     3541 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     3601 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     3661 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     3721 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     3781 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     3841 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgatt
     3901 cgaaatgacc gaccaagcga cgcccaaccg gtatcagctc actcaaaggc ggtaatacgg
     3961 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4021 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4081 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4141 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4201 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4261 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4321 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4381 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4441 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     4501 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4561 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4621 acgcgcagaa aaaaaggatt tcaagaagat cctttgatct tttctacggg gtctgacgct
     4681 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     4741 acctagatcc ttttatagtc cggaatcgtc acacaaaaaa ccaacacaca gatgtaatga
     4801 aaataaagat attttattgc ggccatcgtg atggctagag tttagccctc ccacacataa
     4861 ccagagggca gcaattcacg aatcccaact gccgtcggct gtccatcact gtccttcact
     4921 atggctttga tcccaggatg cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc
     4981 aagatgcccc tgttctcatt tccgatcgcg acgatacaag tcaggttgcc agctgccgca
     5041 gcagcagcag tgcccagcac cacgagttct gcacaaggtc ccccagtaaa atgatataca
     5101 ttgacaccag tgaagatgcg gccgtcgcta gagagagctg cgctggcgac gctgtagtct
     5161 tcagagatgg ggatgctgtt gattgtagcc gttgctcttt caatgagggt ggattcttct
     5221 tgagacaaag gcttggccat ggtggcgcta gtgtcagaag aatcgagctt tttgcaaaag
     5281 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
     5341 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     5401 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     5461 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     5521 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     5581 ccacacccta actgacacac attccacagc tggttctttc cgcctcagaa ggtacctaac
     5641 caagttcctc tttcagaggt tatttcaggc catggtgctg cgcaagacgc gttaattaag
     5701 acgt
//
