LOCUS       Exported                6163 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(293..300)
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(301..894)
                     /label=BCL2L11
                     /note="BCL2L11"
     misc_feature    complement(896..903)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(904..915)
                     /label=linker
                     /note="linker"
     misc_feature    complement(916..1428)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    1442..1468
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(1469..1485)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(1488..1533)
                     /label=Minimal CMV Promoter
     misc_feature    1511..1533
                     /label=core
                     /note="core"
     promoter        1543..2097
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        2115..2181
                     /label=EM7 bacterial promoter
     CDS             2195..2854
                     /label=Chloramphenicol resistance
     rep_origin      3023..3059
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(3741..3788)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(3812..4606)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(4620..4914)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(4985..6163)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagaggatc cccgggtacc gagcccgaat tcgtttaaac
      301 atgcattctc cacaccaggc ggacaatgta acgtaacagt cgtaagataa ccattcgtgg
      361 gtggtcttcg gctgcttggt aattattcaa aaataccctc cttgcatagt aagcgttaaa
      421 ctcgtctcca atacgccgca actcttgggc gatccatatc tctgggcgca tatctgcagg
      481 ttcagcctgc ctcatggaag ccattgcact gagatagtgg ttgaaggcct ggcaaggagg
      541 acttggggtt tgtgttgatt tgtcacaact catgggtgct gggctcctgt ctgtgtcaaa
      601 agagaaatac ccactggagg atcgagacag cagggaggat cttctcataa agatgaaaag
      661 cggggatctg gtagcaaaag ggccagggct ggcaggtggg gccagcgggc cctgagggct
      721 gccgtggggg cagctgtccc cttcacctcc gtgattgcct tcaggattac cttgtggctc
      781 tgtctgtagg gaggtagggg ccccaggtct gagctgggga ggcctctccg caggctgcaa
      841 ttgtctacct tctcggtcac actcagaact tacatcagaa ggttgctttg ccatggcgat
      901 cgcgccgctc gagcccgcca gaatgcgttc gcacagccgc cagccggtca ctccgttgat
      961 ggttactcgg aacagcaggg agccgtcggg gttgatcagg cgctcgtcga taattttgtt
     1021 gccgttccac agggtccctg ttacagtgat ctttttgccg tcgaacacgg cgatgccttc
     1081 atacggccgt ccgaaatagt cgatcatgtt cggcgtaacc ccgtcgatta ccagtgtgcc
     1141 atagtgcagg atcaccttaa agtgatgatc atccacaggg tacaccacct taaaaatttt
     1201 ttcgatctgg cccatttggt cgccgctcag accttcatac gggatgatga catggatgtc
     1261 gatcttcagc ccattttcac cgctcaggac aatcctttgg atcggagtta cggacacccc
     1321 gagattctga aacaaactgg acacacctcc ctgttcaagg acttggtcca ggttgtagcc
     1381 ggctgtctgt cgccagtccc caacgaaatc ttcgagtgtg aagaccatgg tgagcgctag
     1441 ctgcaaaaag aacaagtagc ttgtattcta tagtgtcacc taaatgggcg atctgacggt
     1501 tcactaaacc agctctgctt atataggcct ccctggcgcg ccactagtta ttaatagtaa
     1561 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     1621 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     1681 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     1741 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
     1801 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
     1861 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     1921 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     1981 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     2041 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtcaaa
     2101 tcgaattcta atactgttga caattaatca tcggcatagt atatcggcat agtataatac
     2161 gacaaggtga ggaactaaac ccaggaggca gatcatggag aaaaaaatca ctggatatac
     2221 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     2281 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     2341 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     2401 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     2461 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     2521 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     2581 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     2641 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     2701 cgttttcact atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     2761 tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2821 acagtactgc gatgagtggc agggcggggc gtaagcggga ctctggggtt cgaaatgacc
     2881 gaccaagcga cgcccaaccg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2941 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3001 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3061 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3121 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3181 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3241 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3301 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3361 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3421 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     3481 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3541 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3601 aaaaaggatt tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3661 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3721 ttttatagtc cggaatcgtc acacaaaaaa ccaacacaca gatgtaatga aaataaagat
     3781 attttattgc ggccatcgtg atggctagag ttcagaagaa ctcgtcaaga aggcgataga
     3841 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     3901 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     3961 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     4021 tattcggcaa gcaggcatcg ccgtgggtca cgacgagatc ctcgccgtcg ggcatgcgcg
     4081 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     4141 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     4201 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     4261 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     4321 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     4381 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg
     4441 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     4501 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     4561 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatggtg gcatcgattc
     4621 ccggtcaaca tccagtacac ctccggcacc accggcttcc ccaagggcgc cacgctctcc
     4681 caccacaaca tcctcaacaa cggctactgg gtgggccgca cggtcggcta cacggagcag
     4741 gaccgggtgt gtctgccggt gcccttctac cactgcttcg gcatggtcat gggcaacttg
     4801 ggcgccacct cccacgccgc ctgcatcgtc atcccggccc cgtccttcga gccggcggcg
     4861 acgctggagg cggtgcagcg ggagaggtgc acgtccctgt acggcgtccc cacgaagccg
     4921 aattccacca cactggacta gatgcatgct cgacagtgtg atggatatct gcagaattcg
     4981 gctttcacga cacctgaaat ggaagaaaaa aactttgaac cactgtctga ggcttgagaa
     5041 tgaaccaaga tccaaactca aaaagggcaa attccaagga gaattacatc aagtgccaag
     5101 ctggcctaac ttcagtctcc acccactcag tgtggggaaa ctccatcgca taaaacccct
     5161 ccccccaacc taaagacgac gtactccaaa agctcgagaa ctaatcgagg tgcctggacg
     5221 gcgcccggta ctccgtggag tcacatgaag cgacggctga ggacggaaag gcccttttcc
     5281 tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg cgtcctccat tttgagctgc
     5341 ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc aactggtgcc gaccgggcca
     5401 gccttgccgc ccagggcggg gcgatacacg gcggcgcgag gccaggcacc agagcaggcc
     5461 ggccagcttg agactacccc cgtccgattc tcggtggccg cgctcgcagg ccccgcctcg
     5521 ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca aaaaccgaaa
     5581 taccagtgtg cagatcttgg cccgcattta caagactatc ttgccagaaa aaaagcgtcg
     5641 cagcaggtca tcaaaaattt taaatggcta gagacttatc gaaagcagcg agacaggcgc
     5701 gaaggtgcca ccagattcgc acgcggcggc cccagcgccc aggccaggcc tcaactcaag
     5761 cacgaggcga aggggctcct taagcgcaag gcctcgaact ctcccaccca cttccaaccc
     5821 gaagctcggg atcaagaatc acgtactgca gccaggtgga agtaattcaa ggcacgcaag
     5881 ggccataacc cgtaaagagg ccaggcccgc gggaaccaca cacggcactt acctgtgttc
     5941 tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt atatacggtt
     6001 ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc cagtttaccc
     6061 cgcgccacct tctctaggca ccggttcaat tgccgacccc tccccccaac ttctcgggga
     6121 ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gcc
//
