LOCUS       Exported                9559 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-31Kp-PIK3C2B      9559 bp    DNA   circular          
             5/18/2017

            DEFINITION  Current Name: pF-31Kp - PIK3C2B
FEATURES             Location/Qualifiers
     source          1..9559
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     CDS             1065..1577
                     /label=NanoLuc
     misc_feature    1578..1589
                     /label=N Terminal NanoLuc linker
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1598..6524
                     /label=PIK3C2B
     misc_feature    3634..3639
                     /label=EcoICR I
     misc_feature    3770..3775
                     /label=EcoICR I
     misc_feature    4166..4171
                     /label=EcoICR I
     misc_feature    5702..5707
                     /label=EcoICR I
     misc_feature    6375..6380
                     /label=EcoICR I
     misc_feature    6525..6532
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    6533..6662
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    6684..6905
                     /label=Sv40 late polyadenylation signal
     promoter        7004..7422
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        7430..7496
                     /label=EM7 bacterial promoter
     CDS             7510..8304
                     /label=Neo-Kan Resistance
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    8368..8416
                     /label=Synthetic PolyAdenylation Signal
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgcttt cgaaggagat agaaccatgt
     1621 cttcgactca gggcaatggg gaacactgga agtccctgga gtcagtgggc atcagccgca
     1681 aagagctagc gatggccgaa gccctgcaga tggagtatga tgccctgtcc cggctccggc
     1741 atgacaagga ggagaacaga gccaagcaga acgcagaccc ctctctcatc agctgggatg
     1801 agcctggggt agacttttac agcaagccag caggaaggcg gaccgacctc aagctgttac
     1861 gcggtctctc tggctctgat cctaccctta actacaactc actatcccca caggaagggc
     1921 cgcccaacca ctctacctcc caagggccac agcctggctc agatccctgg cccaaaggct
     1981 ccctgtctgg agactatctc tacatttttg atggttcaga tgggggagtc tcttcgtccc
     2041 caggaccagg ggacatagag ggctcttgca agaaactatc cccacctcct ctgcctcccc
     2101 gagcttctat ctgggatacc cctcccctgc ctcccagaaa ggggtccccc tcatcctcca
     2161 agatctccca gcccagtgac atcaacactt tctctttggt cgaacaattg ccaggcaaac
     2221 tgctagagca tcggatccta gaagaggaag aggtgctggg aggtgggggt caggggcgcc
     2281 tactggggtc tgtggactat gatggtatca atgatgcaat tactaggctc aacttgaaat
     2341 cgacctatga tgcggagatg ttgcgggatg ccaccagggg ctggaaggag ggccgagggc
     2401 cgctggactt cagcaaagac acctctggaa aacccgtggc caggagcaag actatgcccc
     2461 ctcaggtgcc cccccgcacc tatgcctccc gctatggcaa ccgaaagaat gcgacgcctg
     2521 gcaagaaccg ccggatttct gcagccccgg tgggctcccg gccccacact gttgccaatg
     2581 gccatgagtt gtttgaggtc tcagaagaga gagatgagga ggttgctgca ttttgccaca
     2641 tgctggatat ccttcgatct ggctctgaca tccaagacta cttcctcact ggctatgtct
     2701 ggagtgctgt cacccctagc ccagagcacc tcggggatga ggtcaacctg aaggtgactg
     2761 tgttgtgtga caggcttcaa gaggcactca ctttcacctg caactgttcc tccactgtag
     2821 acttgcttat ctaccagacc ctgtgctaca cccatgatga cctgaggaat gtggacgtgg
     2881 gtgactttgt gctaaagccc tgcgggctgg aggagttcct gcagaacaag catgccttgg
     2941 gcagtcatga gtacatccaa tactgccgca agtttgacat tgacattcgg ctacagctga
     3001 tggagcagaa ggttgtgcgc agtgacctgg cccggacggt gaatgatgac cagagcccct
     3061 ccaccttgaa ctacctcgtc catctccaag agaggcctgt caagcagacc atcagcaggc
     3121 aggccctgag tcttctgttc gacacttacc acaatgaggt ggatgccttc ctgctggctg
     3181 atggagactt cccactgaag gctgacaggg tggtccagtc cgtcaaggcc atctgcaacg
     3241 ccctggccgc cgtggaaacc cctgagatca ccagtgctct caaccagctg cccccctgcc
     3301 cctcccgcat gcagcctaaa attcagaagg atcccagtgt cttggctgtg agggaaaacc
     3361 gagagaaggt cgtggaagcc ctgaccgctg ccatcttgga cctggtggag ctgtactgca
     3421 acacattcaa cgcagacttc cagacggcag tgcccgggag ccgcaagcat gacctggtcc
     3481 aggaggcctg ccatttcgcc aggtccctgg ccttcactgt ctatgccacc caccgcatcc
     3541 ccatcatctg ggctaccagc tatgaagatt tctacctctc ctgctccctc agccatggcg
     3601 gcaaggagct gtgcagcccc ctgcagaccc gaagagctca cttctccaag tacctcttcc
     3661 acctcatcgt ctgggaccag cagatctgct tcccagtgca ggtgaaccgg ctgcctcggg
     3721 agacactgct gtgtgccact ctctatgctc tgcccatccc cccaccgggg agctcctcag
     3781 aggccaataa gcagcggcgg gtgcctgaag ccctgggctg ggtcactacc ccactcttca
     3841 acttcaggca ggtcctgacc tgtggccgga agcttctggg tttgtggcca gcaacacagg
     3901 aaaatcccag cgcccgttgg agtgcaccta atttccacca gccagacagt gtcatcctgc
     3961 agattgactt ccccacctcg gcctttgaca tcaagttcac cagcccccct ggagacaagt
     4021 tcagcccccg ctatgagttt ggcagcctcc gggaagaaga ccagcgcaag cttaaagaca
     4081 tcatgcagaa agagtccttg tactggctca ctgatgctga caagaagcgc ctgtgggaga
     4141 agcgatatta ctgccactcg gaggtgagct cgctccccct ggtgctcgcc agcgccccca
     4201 gctgggagtg ggcttgcctg cctgacatct atgttctcct gaagcagtgg acccacatga
     4261 accaccagga tgccctgggg ctcctgcatg ccaccttccc ggaccaggag gtgcgtcgta
     4321 tggctgtgca gtggattggc tcactctcag atgctgagct gctagactac ctgccccagc
     4381 tggtacaggc cctgaagtat gaatgctacc tggacagccc gttggtgcgc ttcctcctga
     4441 aacgagctgt gtctgacttg agagtgactc actacttctt ctggttactg aaggacggcc
     4501 tcaaggactc tcagttcagc atccgctacc agtatctgct ggcagcctta ctgtgctgct
     4561 gtggcaaggg gctgagagaa gagtttaacc gccagtgctg gcttgtcaat gccctggcca
     4621 aactggccca gcaggtccgg gaggcagccc catctgcaag gcagggaatc ctccgcacgg
     4681 gcctggagga ggtgaagcag ttctttgccc tcaatggctc gtgccgcttg ccactcagcc
     4741 ccagtctgct ggttaaggga attgtgccca gggactgttc ctacttcaac tccaatgctg
     4801 tccccctcaa actctccttc caaaatgtgg atcccctggg tgagaacatc cgtgtcatct
     4861 tcaagtgtgg ggacgacctt cgccaggaca tgctaacgct gcagatgatt cgcatcatga
     4921 gcaagatctg ggtccaggag gggctggaca tgcgcatggt catcttccgc tgcttctcca
     4981 ccggccgggg cagagggatg gtggagatga tccctaatgc tgagaccctg cgtaagatcc
     5041 aggtggagca tggggtgacc ggctcgttca aggaccggcc cctggcagac tggctgcaga
     5101 aacacaaccc tggggaggac gagtatgaga aggctgtgga gaactttatc tactcctgcg
     5161 ctggctgctg cgtggccacg tacgtcttgg gcatctgtga ccgacataat gacaacatca
     5221 tgctgaagac cactggtcac atgttccaca ttgattttgg ccgcttcctg ggccatgccc
     5281 agatgtttgg caacatcaag cgggaccgtg ccccctttgt cttcacctcg gacatggcgt
     5341 atgtcatcaa cgggggtgac aagccttcca gccgcttcca tgattttgtt gacctttgct
     5401 gccaagccta caacctcatt cgcaagcaca cccacctctt cctcaacctt ctgggcctga
     5461 tgttgtcctg tgggatccct gaactctcag acctggagga cctcaagtat gtgtacgatg
     5521 ccctgaggcc tcaggataca gaggccaatg ccactaccta cttcactagg ttgattgagt
     5581 ccagcctggg cagtgtagcc acaaagctca attttttcat ccataatctg gctcagatga
     5641 agttcacggg ctcagatgac cggctgaccc tctcctttgc ctcccgaaca cacactctca
     5701 agagctctgg ccgaatcagt gatgttttcc tctgccgcca tgagaagatc ttccacccca
     5761 acaaaggcta tatatatgtg gtaaaggtga tgcgagagaa cactcacgag gccacctaca
     5821 tccagcggac ctttgaggag ttccaggaat tacacaataa gttgcggctg ctcttccctt
     5881 cttcccactt gcccagcttc cctagtcgct tcgtgatcgg ccgctcccgg ggagaggcgg
     5941 tggccgagcg gcggagggag gagctaaacg gttacatctg gcacttgatc cacgcacccc
     6001 ctgaggtggc cgagtgtgat ttggtgtaca ccttcttcca cccactgccc cgggatgaga
     6061 aggctatggg caccagccca gctcctaagt cctcagatgg cacatgggcc cggcccgtcg
     6121 gaaaggtggg aggggaggtg aagctgtcca tctcctacaa aaacaataaa ctcttcatca
     6181 tggtgatgca tattcggggc ttgcaactgc tccaggatgg aaatgaccct gacccctatg
     6241 tgaaaattta cctccttcct gaccctcaga aaaccactaa gaggaaaacc aaagtggccc
     6301 ggaaaacctg caatcctacc tacaatgaga tgttggtata tgatgggatc cccaagggtg
     6361 acctgcagca gcgggagctc cagctgagcg tgctgagtga gcagggattc tgggagaacg
     6421 tcctcctcgg tgaggtgaac atccgcctgc gagagctgga cctggctcag gagaagaccg
     6481 gctggttcgc cctgggatct cgaagtcatg gcaccttgta cgtagtttaa acgaattcgg
     6541 gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagctga tccggctgct
     6601 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
     6661 ccccttgggg cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     6721 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     6781 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     6841 gtttcaggtt cagggggaga tgtgggaggt ttttttaagc aagtaaaacc tctacaaatg
     6901 tggtaaaatc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     6961 ccttacgcat ctgtgcggta tttcacaccg catacgcgga tctgcgcagc accatggcct
     7021 gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg
     7081 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7141 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     7201 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     7261 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7321 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7381 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttaattaact gttgacaatt
     7441 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaacccagg
     7501 aggcagatca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     7561 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     7621 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     7681 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     7741 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     7801 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     7861 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     7921 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     7981 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     8041 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     8101 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     8161 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     8221 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     8281 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     8341 cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt acatctgtgt
     8401 gttggttttt tgtgtgaatc gatagcgata aggatccgct catgcgatga taagctgtca
     8461 aacatgagaa ttacaactta tatcgtatgg ggctgacttc aggtgctaca tttgaagaga
     8521 taaattgcac tgaaatctag aaatatttta tctgattaat aagatgatct tcttgagatc
     8581 gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt
     8641 tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc
     8701 agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact
     8761 cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg
     8821 gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc
     8881 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
     8941 caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
     9001 cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     9061 ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa
     9121 cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa
     9181 tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag
     9241 tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg caccggtgca
     9301 gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg ccaacatagt
     9361 aagccagtat acactccgct agcgctgatg tccggcggtg cttttgccgt tacgcaccac
     9421 cccgtcatga gattacgcgt tttagcggtg cgtacaatta agggattatg gtaaatccac
     9481 ttactgtctg ccctcgtagc catcgagata aaccgcagta ctccggccac gatgcgtccg
     9541 gcgtagagga tcgagatct
//
