LOCUS       Exported                8285 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nn5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nn5K3
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1065..1577
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    1578..1589
                     /label=linker
                     /note="linker"
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     CDS             1599..5240
                     /label=DGKD sequence
     misc_feature    5241..5248
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    5400..5621
                     /label=Sv40 late polyadenylation signal
     promoter        5720..6138
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        6146..6212
                     /label=EM7 bacterial promoter
     CDS             6226..7020
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    7094..7142
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    7185..8107
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    7362
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat ggcggcggcg gcgggcgccc
     1621 ctccgccggg tcccccgcaa ccgcctccgc cgccgccgcc cgaggagtcg tccgacagcg
     1681 agcccgaggc ggagcccggc tccccacaga agctcatccg caaggtgtcc acgtcgggtc
     1741 agatccgaca gaagaccatc atcaaagagg ggatgctgac caaacagaac aattcattcc
     1801 agcgatcaaa aaggagatac tttaagcttc gagggcgaac gctttactat gccaaaacgg
     1861 caaagtcaat catatttgat gaggtggatc tgacagatgc cagcgtagct gaatccagta
     1921 ccaaaaacgt caacaacagt tttacggtca taactccatg caggaagctc atcttgtgtg
     1981 ctgataacag aaaagaaatg gaagattgga ttgcagcatt aaagactgtg cagaacaggg
     2041 agcactttga gcccacccag tacagcatgg accacttctc agggatgcac aattggtacg
     2101 cctgttccca cgcgaggccg acctactgca atgtgtgccg tgaggctctg tctggggtca
     2161 cgtcgcacgg gctgtcctgc gaggtgtgca aatttaaggc ccacaagcgc tgtgctgtgc
     2221 gtgcaaccaa taactgcaag tggaccacac tggcctcgat cgggaaggac atcattgaag
     2281 atgcagatgg gattgcaatg ccccaccagt ggttggaagg aaacctacct gtgagcgcca
     2341 agtgcactgt gtgcgacaag acctgtggca gtgtgctgcg cctgcaggac tggcgctgcc
     2401 tctggtgcaa ggccatggtt cacacatcgt gtaaagaatc cttgctgacc aagtgcccac
     2461 ttggcctgtg caaagtgtca gtcatcccac ccacggctct caacagcatc gactccgatg
     2521 ggttctggaa ggccagctgt cctccttctt gcacaagccc actgttggtc ttcgtcaatt
     2581 caaaaagtgg ggacaaccag ggtgtgaagt tcctcagaag attcaaacag ctactaaacc
     2641 ccgcccaggt cttcgacctc atgaacggag gcccacacct cggcttacgg ttattccaga
     2701 agtttgacac attccggatt ctggtttgtg gcggggatgg aagtgttggc tgggtcctct
     2761 ccgaaatcga cagcctcaac cttcataaac agtgtcagct gggagtgctg ccgctcggca
     2821 cagggaacga cttggcccga gtactgggct ggggctcagc ctgcgatgac gacacccagc
     2881 tcccccagat cttggagaag ttggagagag ccagcaccaa gatgctggac aggtggagcg
     2941 tcatggcata cgaggccaag ctcccccggc aggcctcctc ctctaccgtc accgaagact
     3001 tcagcgagga ttccgaggta cagcagattc tcttctatga agactcggtt gcagcccacc
     3061 tttctaaaat cctcacctcg gaccagcact cggtggtcat ctcctcggcc aaagtgctct
     3121 gtgagacggt gaaggacttc gtggcacggg tggggaaggc ctatgagaag acgaccgaga
     3181 gctcggagga gtcagaggtc atggccaaga agtgctctgt cctgaaagag aagctggatt
     3241 cccttctcaa gaccttggac gatgagtccc aggcctcgtc ctctctgccc aacccgcccc
     3301 ccaccattgc cgaggaggct gaagatggag atgggtcggg cagcatctgc ggttccaccg
     3361 gagaccgctt ggtggcatca gcttgcccgg cccggccgca gatattccgg cctcgagaac
     3421 agctcatgct gagagccaac agcctgaaga aagcaattcg tcagatcata gaacacacag
     3481 aaaaagctgt cgatgagcag aatgcccaga cccaggagca ggagggcttc gtcctgggcc
     3541 tctctgagtc agaggagaag atggaccaca gagtgtgccc accactgtcc cacagcgaga
     3601 gcttcggggt ccccaagggg aggagccagc gcaaagtgtc gaaatctccg tgtgaaaagc
     3661 tgatcagcaa agggagtctg tccctaggca gttctgcttc ccttccgccc cagccgggaa
     3721 gccgggacgg cctgcctgcg ctcaacacca agatcctgta cccaaatgtc cgggctggaa
     3781 tgtctggttc cttacccggt ggctcagtca tcagtcgcct gttaattaat gctgatccct
     3841 tcaactctga accagaaacc ctagagtatt acacggagaa atgtgtcatg aacaactatt
     3901 ttggcattgg cctggatgcg aagatatccc tggactttaa caacaagcgc gatgagcacc
     3961 cagagaagtg caggagccga accaagaaca tgatgtggta tggagttctt ggaaccaaag
     4021 agttgctgca cagaacctac aagaacctgg agcaaaaggt cttgctggag tgtgacgggc
     4081 gacccatccc actccccagt cttcagggaa ttgctgtcct taacattccc agctatgccg
     4141 gaggaaccaa cttctggggg ggtaccaagg aagatgatac tttcgcagct ccatcattcg
     4201 atgacaagat tctggaggtg gtcgccgtgt tcggcagcat gcagatggcc gtctctcgag
     4261 tcatcaggct acagcatcat cggatcgccc agtgtcgcac ggtgaagatc tccatccttg
     4321 gggatgaggg cgtgcctgtg caggtggacg gagaggcctg ggtccagccg ccagggtaca
     4381 ttcggattgt ccacaagaac cgggcacaga cactgaccag agacagggca tttgagagca
     4441 ccctgaagtc ctgggaagac aagcagaagt gcgagctgcc ccgccctcca tcctgttccc
     4501 tgcacccgga gatgctgtcc gaggaggagg ccacccagat ggaccagttt gggcaggcag
     4561 caggggtcct cattcacagt atccgagaaa tagctcagtc tcaccgggac atggagcagg
     4621 aactggccca cgccgtcaat gccagctcca agtccatgga ccgtgtgtat ggcaagccca
     4681 gaaccacaga ggggctcaac tgcagcttcg tcctggaaat ggtgaataac ttcagagctc
     4741 tgcgcagtga gacggagctg ctgctgtctg ggaagatggc cctgcagctg gatccgcctc
     4801 agaaggagca gctggggagt gctcttgccg agatggaccg acagctcagg aggctggcag
     4861 acaccccgtg gctctgccag tccgcagagc ccggcgacga agagagtgtg atgctggatc
     4921 ttgccaagcg cagtcgcagt ggtaaattcc gcctcgtgac caagtttaaa aaggagaaaa
     4981 acaacaagaa caaagaagct cacagtagcc tgggagcccc ggttcacctc tgggggacag
     5041 aggaggttgc tgcctggctg gagcacctca gtctctgtga gtataaggac atcttcacac
     5101 ggcacgacat ccggggctct gagctcctgc acctggagcg gagggacctc aaggacctgg
     5161 gcgtgaccaa ggtgggccac atgaagagga tcctgtgtgg catcaaggag ctgagccgca
     5221 gcgcccccgc cgtcgaggcc gtttaaacga attcgggctc ggtacccggg gatcctctag
     5281 agtcgacctg caggcatgca agctgatccg gctgctaaca aagcccgaaa ggaagctgag
     5341 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcggc cgcttcgagc
     5401 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     5461 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     5521 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     5581 ggaggttttt ttaagcaagt aaaacctcta caaatgtggt aaaatcgaat tttaacaaaa
     5641 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5701 acaccgcata cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact
     5761 tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     5821 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5881 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5941 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     6001 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6061 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6121 ggcttttgca aaaagcttaa ttaactgttg acaattaatc atcggcatag tatatcggca
     6181 tagtataata cgacaaggtg aggaactaaa cccaggaggc agatcatgat tgaacaagat
     6241 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6301 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6361 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     6421 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6481 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6541 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6601 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6661 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6721 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     6781 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6841 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6901 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6961 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     7021 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgatggc
     7081 cgdgkgtnrt nmcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     7141 tgaatcgata gcgataagga tccgctcatg cgatgataag ctgtcaaaca tgagaattac
     7201 aacttatatc gtatggggct gacttcaggt gctacatttg aagagataaa ttgcactgaa
     7261 atctagaaat attttatctg attaataaga tgatcttctt gagatcgttt tggtctgcgc
     7321 gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc
     7381 tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc accaaaactt
     7441 gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc taaatcaatt
     7501 accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact caagacgata
     7561 gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac agtccagctt
     7621 ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata
     7681 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccgc
     7741 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
     7801 gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg
     7861 ccctctcact tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc
     7921 gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
     7981 agcggaatat atcctgtatc acatattctg ctgacgcacc ggtgcagcct tttttctcct
     8041 gccacatgaa gcacttcact gacaccctca tcagtgccaa catagtaagc cagtatacac
     8101 tccgctagcg ctgatgtccg gcggtgcttt tgccgttacg caccaccccg tcatgagatt
     8161 acgcgtttta gcggtgcgta caattaaggg attatggtaa atccacttac tgtctgccct
     8221 cgtagccatc gagataaacc gcagtactcc ggccacgatg cgtccggcgt agaggatcga
     8281 gatct
//
