LOCUS       Exported                7668 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nc5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nc5K3
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..3989
                     /label=NLRX1
                     /note="NLRX1"
     misc_feature    3996..4010
                     /label=linker
                     /note="linker"
     misc_feature    4011..4520
                     /label=NanoLuc
                     /note="NanoLuc"
     polyA_signal    4657..4878
                     /label=Sv40 late polyadenylation signal
     promoter        4977..5395
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        5403..5469
                     /label=EM7 bacterial promoter
     CDS             5483..6277
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    6341..6389
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      6625..6661
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgagg tggggccacc
     1081 atttgcccag ggcctcttgg ggctctggtt ttagaagagc actccagcga ccagatgatc
     1141 gtatcccctt cctgatccac tggagttggc cccttcaagg ggagcgtccc tttgggcccc
     1201 ctagggcctt tatacgccac cacggaagct cggtagatag cgctccccca cccgggaggc
     1261 atggacggct gttccccagc gcctctgcaa ctgaagctat acagcggcac cgccggaacc
     1321 tggctgagtg gttcagccgg ctgcccaggg aggagcgcca gtttggccca acctttgccc
     1381 tagacacggt ccacgttgac cctgtgatcc gcgagagtac ccctgatgag ctacttcgcc
     1441 cacccgcgga gctggccctg gagcatcagc caccccaggc cgggctcccc ccactggcct
     1501 tgtctcagct ctttaacccg gatgcctgtg ggcgccgggt gcagacagtg gtgctgtatg
     1561 ggacagtggg cacaggcaag agcacgctgg tgcgcaagat ggttctggac tggtgttatg
     1621 ggcggctgcc ggccttcgag ctgctcatcc ccttctcctg tgaggacctg tcatccctgg
     1681 gccctgcccc agcctccctg tgccaacttg tggcccagcg ctacacgccc ctgaaggagg
     1741 ttctgcccct gatggctgct gctgggtccc acctcctctt tgtgctccat ggcttagagc
     1801 atctcaacct cgacttccgg ctggcaggca cgggactttg tagtgacccg gaggaaccgc
     1861 aggaaccagc tgctatcatc gtcaacctgc tgcgcaaata catgctgcct caggccagca
     1921 ttctggtgac cactcggccc tctgccattg gccgtatccc cagcaagtac gtgggccgct
     1981 atggtgagat ctgcggtttc tctgatacca acctgcagaa gctctacttc cagctccgcc
     2041 tcaaccagcc gtactgcggg tatgccgttg gcggttcagg tgtctctgcc acaccagctc
     2101 agcgtgacca cctggtgcag atgctctccc ggaacctgga ggggcaccac cagatagccg
     2161 ctgcctgctt cctgccgtcc tattgctggc tcgtttgtgc caccttgcac ttcctgcatg
     2221 cccccacgcc tgctgggcag acccttacaa gcatctatac cagcttcctg cgcctcaact
     2281 tcagcgggga aaccctggac agcactgacc cctccaattt gtccctgatg gcctatgcag
     2341 cccgaaccat gggcaagttg gcctatgagg gggtgtcctc ccgcaagacc tacttctctg
     2401 aagaggatgt ctgtggctgc ctggaggctg gcatcaggac ggaggaggag tttcagctgc
     2461 tgcacatctt ccgtcgggat gccctgaggt ttttcctggc cccatgtgtg gagccagggc
     2521 gtgcaggcac cttcgtgttc accgtgcccg ccatgcagga atacctggct gccctctaca
     2581 ttgtgctggg tttgcgcaag acgaccctgc aaaaggtggg caaggaagtg gctgagctcg
     2641 tgggccgtgt tggggaggac gtcagcctgg tactgggcat catggccaag ctgctgcctc
     2701 tgcgggctct gcctctgctc ttcaacctga tcaaggtggt tccacgagtg tttgggcgca
     2761 tggtgggtaa aagccgggag gcggtggctc aggccatggt gctggagatg tttcgagagg
     2821 aggactacta caacgatgat gttctggacc agatgggcgc cagtatcctg ggcgtggagg
     2881 gcccccggcg ccacccagat gagccccctg aggatgaagt cttcgagctc ttccccatgt
     2941 tcatgggggg gcttctctct gcccacaacc gagctgtgct agctcagctt ggctgcccca
     3001 tcaagaacct ggatgccctg gagaatgccc aggccatcaa gaagaagctg ggcaagctgg
     3061 gccggcaggt gctgccccca tcagagctcc ttgaccacct cttcttccac tatgagttcc
     3121 agaaccagcg cttctccgct gaggtgctca gctccctgcg tcagctcaac ctggcaggtg
     3181 tgcgcatgac accagtcaag tgcacagtgg tggcagctgt gctgggcagc ggaaggcatg
     3241 ccctggatga ggtgaacttg gcctcctgcc agctagatcc tgctgggctg cgcacactcc
     3301 tgcctgtctt cctgcgtgcc cggaagctgg gcttgcaact caacagcctg ggccctgagg
     3361 cctgcaagga cctccgagac ctgttgctgc atgaccagtg ccaaattacc acactgcggc
     3421 tgtccaacaa cccgctgacg gcggcaggtg ttgccgtgct aatggagggg ctggcaggaa
     3481 acacctcagt gacgcacctg tccctgctgc acacgggcct tggggacgaa ggcctggagc
     3541 tgctggctgc ccagctggac cgcaaccggc agctgcagga gctgaacgtg gcgtacaacg
     3601 gtgctggtga cacagcggcc ctggccctgg ccagagctgc ccgggagcac ccttccctgg
     3661 aactgctaca cctctacttc aatgagctga gctcagaggg ccgccaggtc ttgcgagact
     3721 tggggggtgc tgctgaaggt ggtgcccggg tggtggtgtc actgacagag gggacggcgg
     3781 tgtcagaata ctggtcagtg atcctcagtg aagtccagcg gaacctcaat agctgggatc
     3841 gggcccgggt tcagcgacac cttgagctcc tactgcggga tctggaagat agccggggtg
     3901 ccacccttaa tccttggcgc aaggcccagc tgctgcgagt ggagggcgag gtcagggccc
     3961 tcctggagca gctgggaagc tctggaagcg tttctctcgg ctcgagcggc gtcttcacac
     4021 tcgaagattt cgttggggac tggcgacaga cagccggcta caacctggac caagtccttg
     4081 aacagggagg tgtgtccagt ttgtttcaga atctcggggt gtccgtaact ccgatccaaa
     4141 ggattgtcct gagcggtgaa aatgggctga agatcgacat ccatgtcatc atcccgtatg
     4201 aaggtctgag cggcgaccaa atgggccaga tcgaaaaaat ttttaaggtg gtgtaccctg
     4261 tggatgatca tcactttaag gtgatcctgc actatggcac actggtaatc gacggggtta
     4321 cgccgaacat gatcgactat ttcggacggc cgtatgaagg catcgccgtg ttcgacggca
     4381 aaaagatcac tgtaacaggg accctgtgga acggcaacaa aattatcgac gagcgcctga
     4441 tcaaccccga cggctccctg ctgttccgag taaccatcaa cggagtgacc ggctggcggc
     4501 tgtgcgaacg cattctggcg taaggccgcg actctagagt cgacctgcag gcatgcaagc
     4561 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     4621 ataactagca taaccccttg gggcggccgc ttcgagcaga catgataaga tacattgatg
     4681 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     4741 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     4801 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttta agcaagtaaa
     4861 acctctacaa atgtggtaaa atcgaatttt aacaaaatat taacgcttac aatttcctga
     4921 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc
     4981 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg
     5041 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5101 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5161 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     5221 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5281 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5341 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttaatta
     5401 actgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     5461 aactaaaccc aggaggcaga tcatgattga acaagatgga ttgcacgcag gttctccggc
     5521 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5581 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5641 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     5701 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     5761 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5821 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5881 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5941 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     6001 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     6061 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     6121 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     6181 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6241 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6301 accgaccaag cgacgcccaa cctgccatca cgatggccgc aataaaatat ctttattttc
     6361 attacatctg tgtgttggtt ttttgtgtga atcgatagcg ataaggatcc tctttgcgct
     6421 tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt
     6481 ctgcggactg gctttctacc cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6541 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6601 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6661 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6721 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6781 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6841 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6901 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6961 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7021 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     7081 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7141 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7201 aaaaaaagga tttcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7261 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7321 ccttttatag tccggaaata caggaacgca cgctggatgg cccttcgctg ggatggtgaa
     7381 accatgaaaa atggcagctt cagtggatta agtgggggta atgtggcctg taccctctgg
     7441 ttgcataggt attcatacgg ttaaaattta tcaggcgcga ttgcggcagt ttttcgggtg
     7501 gtttgttgcc atttttacct gtctgctgcc gtgatcgcgc tgaacgcgtt ttagcggtgc
     7561 gtacaattaa gggattatgg taaatccact tactgtctgc cctcgtagcc atcgagataa
     7621 accgcagtac tccggccacg atgcgtccgg cgtagaggat cgagatct
//
