LOCUS       Exported                6344 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nn5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nn5K3
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1065..1577
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    1578..1589
                     /label=linker
                     /note="linker"
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     CDS             1599..3299
                     /label=DGKE sequence
     misc_feature    3300..3307
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    3459..3680
                     /label=Sv40 late polyadenylation signal
     promoter        3779..4197
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4205..4271
                     /label=EM7 bacterial promoter
     CDS             4285..5079
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5153..5201
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    5244..6166
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    5421
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat ggaagcggag aggcggccgg
     1621 cgccgggctc gccctccgag ggcctgtttg cggacgggca cctgatcttg tggacgctgt
     1681 gctcggtcct gctgccggtg ttcatcacct tctggtgtag cctccagcgg tcgcgccggc
     1741 agctgcaccg cagggacatc ttccgcaaga gcaagcacgg gtggcgcgac acggacctgt
     1801 tcagccagcc cacctactgc tgcgtgtgcg cgcagcacat tctgcagggc gccttctgcg
     1861 actgctgcgg gctccgcgtg gacgagggct gcctcaggaa ggccgacaag cgcttccagt
     1921 gcaaggagat tatgctcaag aatgacacca aggtcctgga cgccatgccc caccactgga
     1981 tccggggcaa cgtgcccctg tgcagttact gtatggtttg caagcagcag tgtggctgtc
     2041 aacccaagct ttgcgattac aggtgcattt ggtgccagaa aacagtacat gatgagtgca
     2101 tgaaaaatag tttaaagaat gaaaaatgtg attttggaga attcaaaaac ctaatcattc
     2161 caccaagtta tttaacctcc attaatcaga tgcgtaaaga caaaaaaaca gattatgaag
     2221 tgctagcctc taagcttgga aagcagtgga ccccattaat aatcctggcc aactctcgta
     2281 gtggaactaa tatgggagaa ggactgttgg gagaatttag gatcttgttg aatccagtcc
     2341 aggtttttga tgtaactaaa actcctccta tcaaagccct acaactctgt actcttctcc
     2401 catattattc agctcgagta cttgtttgtg gaggggatgg gactgtaggg tgggtcctgg
     2461 atgcagttga tgacatgaag attaagggac aagaaaagta cattccacaa gttgcagttt
     2521 tgcctctggg aacaggcaac gatctatcca atacattggg ttggggtaca ggttatgctg
     2581 gagaaattcc agttgcgcag gttttgcgaa atgtaatgga agcagatgga attaaactag
     2641 atcgatggaa agttcaagta acaaataaag gatactacaa cttaagaaaa cccaaggaat
     2701 tcacaatgaa caactatttt tctgttggac ctgatgctct catggctctc aattttcatg
     2761 ctcatcgtga gaaggcacca tctctgtttt ctagcagaat tcttaataag gcggtttact
     2821 tattctatgg aaccaaagat tgtttagtgc aagaatgtaa agatttgaat aaaaaagttg
     2881 agctagaact ggatggtgag cgagtagcac tgcccagctt ggaaggtatt atagttctga
     2941 acatcggata ctggggcggt ggctgcagac tatgggaagg gatgggggac gagacttacc
     3001 ctctagccag gcatgacgat ggtctgctgg aagtcgttgg agtatatggg tctttccact
     3061 gtgctcagat tcaagtaaaa ctggctaatc cttttcgaat aggacaggca catacagtga
     3121 ggctgatttt gaagtgctcc atgatgccaa tgcaggtgga tggggagcct tgggcccaag
     3181 ggccctgcac tgtcaccata actcacaaga cacatgcaat gatgttatat ttctctggag
     3241 aacaaacaga tgatgacatc tctagtactt cggatcaaga agatataaag gcgactgaag
     3301 tttaaacgaa ttcgggctcg gtacccgggg atcctctaga gtcgacctgc aggcatgcaa
     3361 gctgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
     3421 caataactag cataacccct tggggcggcc gcttcgagca gacatgataa gatacattga
     3481 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     3541 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3601 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt taagcaagta
     3661 aaacctctac aaatgtggta aaatcgaatt ttaacaaaat attaacgctt acaatttcct
     3721 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gcggatctgc
     3781 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc
     3841 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3901 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     3961 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4021 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4081 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4141 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttaat
     4201 taactgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     4261 ggaactaaac ccaggaggca gatcatgatt gaacaagatg gattgcacgc aggttctccg
     4321 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     4381 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     4441 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     4501 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4561 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     4621 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     4681 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     4741 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4801 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     4861 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     4921 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     4981 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     5041 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     5101 tgaccgacca agcgacgccc aacctgccat cacgatggcc gdgkgtnrtn mcaataaaat
     5161 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag cgataaggat
     5221 ccgctcatgc gatgataagc tgtcaaacat gagaattaca acttatatcg tatggggctg
     5281 acttcaggtg ctacatttga agagataaat tgcactgaaa tctagaaata ttttatctga
     5341 ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac
     5401 gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac
     5461 cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa
     5521 ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg
     5581 tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5641 gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga
     5701 actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa
     5761 accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc
     5821 tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt
     5881 caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag
     5941 tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg
     6001 cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca
     6061 catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg
     6121 acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgatgtccgg
     6181 cggtgctttt gccgttacgc accaccccgt catgagatta cgcgttttag cggtgcgtac
     6241 aattaaggga ttatggtaaa tccacttact gtctgccctc gtagccatcg agataaaccg
     6301 cagtactccg gccacgatgc gtccggcgta gaggatcgag atct
//
