LOCUS       Exported                6260 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-32Kp-EIF2AK2      6260 bp    DNA   circular          
             5/31/2016

            DEFINITION  Current Name: pF-32Kp - EIF2AK2
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1060..2721
                     /label=EIF2AK2 insert
     misc_feature    complement(2214..2220)
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    2718..2724
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    2724..2738
                     /label=C Terminal NanoLuc linker
     misc_feature    2727..2738
                     /label=N Terminal NanoLuc linker
     CDS             2739..3248
                     /label=C terminal NanoLuc
     polyA_signal    3385..3606
                     /label=Sv40 late polyadenylation signal
     promoter        3705..4123
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(3778..4014)
                     /label=SV40 Enhancer
                     /note="Sequence shown is from SV40 genome. Our vectors 
                     contain sequences with one or two point mutations in first 
                     few bases. These apparently do not affect function. The 
                     sequences present in our vectors are shown to the right."
     rep_origin      4021..4086
                     /label=Sv40 Min Ori
     promoter        4131..4197
                     /label=EM7 bacterial promoter
     CDS             4211..5005
                     /label=Neomycin phosphotransferase coding
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5069..5117
                     /label=Synthetic PolyAdenylation Signal
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggct ggtgatcttt
     1081 cagcaggttt cttcatggag gaacttaata cataccgtca gaagcaggga gtagtactta
     1141 aatatcaaga actgcctaat tcaggacctc cacatgatag gaggtttaca tttcaagtta
     1201 taatagatgg aagagaattt ccagaaggtg aaggtagatc aaagaaggaa gcaaaaaatg
     1261 ccgcagccaa attagctgtt gagatactta ataaggaaaa gaaggcagtt agtcctttat
     1321 tattgacaac aacgaattct tcagaaggat tatccatggg gaattacata ggccttatca
     1381 atagaattgc ccagaagaaa agactaactg taaattatga acagtgtgca tcgggggtgc
     1441 atgggccaga aggatttcat tataaatgca aaatgggaca gaaagaatat agtattggta
     1501 caggttctac taaacaggaa gcaaaacaat tggccgctaa acttgcatat cttcagatat
     1561 tatcagaaga aacctcagtg aaatctgact acctgtcctc tggttctttt gctactacgt
     1621 gtgagtccca aagcaactct ttagtgacca gcacactcgc ttctgaatca tcatctgaag
     1681 gtgacttctc agcagataca tcagagataa attctaacag tgacagtttg aacagttctt
     1741 cgttgcttat gaatggtctc agaaataatc aaaggaaggc aaaaagatct ttggcaccca
     1801 gatttgacct tcctgacatg aaagaaacaa agtatactgt ggacaagagg tttggcatgg
     1861 attttaaaga aatagaatta attggctcag gtggatttgg ccaagttttc aaagcaaaac
     1921 acagaattga cggaaagact tacgttatta aacgtgttaa atataataac gagaaggcgg
     1981 agcgtgaagt aaaagcattg gcaaaacttg atcatgtaaa tattgttcac tacaatggct
     2041 gttgggatgg atttgattat gatcctgaga ccagtgatga ttctcttgag agcagtgatt
     2101 atgatcctga gaacagcaaa aatagttcaa ggtcaaagac taagtgcctt ttcatccaaa
     2161 tggaattctg tgataaaggg accttggaac aatggattga aaaaagaaga ggcgagaaac
     2221 tagacaaagt tttggctttg gaactctttg aacaaataac aaaaggggtg gattatatac
     2281 attcaaaaaa attaattcat agagatctta agccaagtaa tatattctta gtagatacaa
     2341 aacaagtaaa gattggagac tttggacttg taacatctct gaaaaatgat ggaaagcgaa
     2401 caaggagtaa gggaactttg cgatacatga gcccagaaca gatttcttcg caagactatg
     2461 gaaaggaagt ggacctctac gctttggggc taattcttgc tgaacttctt catgtatgtg
     2521 acactgcttt tgaaacatca aagtttttca cagacctacg ggatggcatc atctcagata
     2581 tatttgataa aaaagaaaaa actcttctac agaaattact ctcaaagaaa cctgaggatc
     2641 gacctaacac atctgaaata ctaaggacct tgactgtgtg gaagaaaagc ccagagaaaa
     2701 atgaacgaca cacatgtgtt tctctcggct cgagcggcgt cttcacactc gaagatttcg
     2761 ttggggactg gcgacagaca gccggctaca acctggacca agtccttgaa cagggaggtg
     2821 tgtccagttt gtttcagaat ctcggggtgt ccgtaactcc gatccaaagg attgtcctga
     2881 gcggtgaaaa tgggctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg
     2941 gcgaccaaat gggccagatc gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc
     3001 actttaaggt gatcctgcac tatggcacac tggtaatcga cggggttacg ccgaacatga
     3061 tcgactattt cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg
     3121 taacagggac cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg
     3181 gctccctgct gttccgagta accatcaacg gagtgaccgg ctggcggctg tgcgaacgca
     3241 ttctggcgta aggccgcgac tctagagtcg acctgcaggc atgcaagctg atccggctgc
     3301 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     3361 accccttggg gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     3421 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     3481 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     3541 tgtttcaggt tcagggggag atgtgggagg tttttttaag caagtaaaac ctctacaaat
     3601 gtggtaaaat cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc
     3661 tccttacgca tctgtgcggt atttcacacc gcatacgcgg atctgcgcag caccatggcc
     3721 tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct
     3781 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3841 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     3901 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3961 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4021 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4081 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttaattaac tgttgacaat
     4141 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaacccag
     4201 gaggcagatc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4261 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4321 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     4381 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     4441 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     4501 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4561 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4621 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4681 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     4741 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     4801 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     4861 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     4921 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     4981 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     5041 acgcccaacc tgccatcacg atggccgcaa taaaatatct ttattttcat tacatctgtg
     5101 tgttggtttt ttgtgtgaat cgatagcgat aaggatccgc tcatgcgatg ataagctgtc
     5161 aaacatgaga attacaactt atatcgtatg gggctgactt caggtgctac atttgaagag
     5221 ataaattgca ctgaaatcta gaaatatttt atctgattaa taagatgatc ttcttgagat
     5281 cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt
     5341 ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct tggaggagcg
     5401 cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac
     5461 tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt
     5521 ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg
     5581 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
     5641 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
     5701 gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5761 accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa
     5821 acggctttgc cgcggccctc tcacttccct gttaagtatc ttcctggcat cttccaggaa
     5881 atctccgccc cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga
     5941 gtcagtgagc gaggaagcgg aatatatcct gtatcacata ttctgctgac gcaccggtgc
     6001 agcctttttt ctcctgccac atgaagcact tcactgacac cctcatcagt gccaacatag
     6061 taagccagta tacactccgc tagcgctgat gtccggcggt gcttttgccg ttacgcacca
     6121 ccccgtcatg agattacgcg ttttagcggt gcgtacaatt aagggattat ggtaaatcca
     6181 cttactgtct gccctcgtag ccatcgagat aaaccgcagt actccggcca cgatgcgtcc
     6241 ggcgtagagg atcgagatct
//
