LOCUS       Exported                5849 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-32Kp-STK17A      5849 bp    DNA   circular           
            5/31/2016

            DEFINITION  Current Name: pF-32Kp - STK17A
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..2306
                     /label=STK17A
     misc_feature    2307..2327
                     /label=linker
     CDS             2328..2837
                     /label=NanoLuc
     polyA_signal    2974..3195
                     /label=Sv40 late polyadenylation signal
     promoter        3294..3712
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        3720..3786
                     /label=EM7 bacterial promoter
     CDS             3800..4594
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4658..4706
                     /label=Synthetic PolyAdenylation Signal
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgatc cctttggaga
     1081 agccaggcag cggcggctcc tccccaggcg ccacctcagg ctcgggccgg gcaggccggg
     1141 gtctgagcgg gccgtgccgg ccgccgccgc cgccccaggc ccgcgggctg ttgacagaga
     1201 tacgcgccgt ggtgcgcacc gagcccttcc aggacggcta cagcctgtgc ccgggccggg
     1261 agctgggcag ggggaaattt gcagtggtga gaaaatgtat aaagaaagat tctgggaaag
     1321 aatttgctgc aaagttcatg agaaaaagaa gaaaaggcca agattgtcgg atggaaataa
     1381 ttcatgagat tgctgtactt gaactagcac aagacaatcc ttgggtcatt aatttacatg
     1441 aagtttatga gactgcatca gaaatgatct tagttctgga atatgctgct gggggtgaaa
     1501 tctttgacca gtgtgttgca gacagagaag aagcctttaa agaaaaagat gttcaaagac
     1561 ttatgcgaca gattttagaa ggtgttcact ttttacacac tcgtgatgta gttcatcttg
     1621 atttgaagcc tcagaatatt ctgttgacaa gtgaatctcc attgggtgac attaagattg
     1681 ttgattttgg cctttcaaga atattgaaga acagtgaaga gctccgagaa attatgggta
     1741 cccctgaata tgtggctcct gaaattctta gttatgatcc tataagcatg gcaacagata
     1801 tgtggagcat tggagtgtta acatatgtca tgcttacagg aatatcacct ttcttaggca
     1861 atgataaaca agaaacattc ttaaacatct cacagatgaa tttaagttat tctgaggaag
     1921 aatttgatgt tttgtctgag tcggctgttg atttcatcag gacactttta gttaagaaac
     1981 ctgaagatcg agccactgct gaagaatgtc taaagcaccc ctggttgaca cagagcagta
     2041 ttcaagagcc ttctttcagg atggaaaagg cactagaaga agcaaatgcc ctccaagaag
     2101 gtcattctgt gcctgaaatt aattcggata ccgacaaatc agaaaccgag gaatccattg
     2161 taaccgaaga gttaattgta gttacttcat atactctagg acaatgcaga cagtctgaaa
     2221 aagagaaaat ggagcaaaag gccatttcca aacgatttaa atttgaggaa cctttgctac
     2281 aagaaattcc aggagaattt atctacgttt ctctcggctc gagcggcgtc ttcacactcg
     2341 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     2401 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     2461 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     2521 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     2581 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     2641 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     2701 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     2761 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     2821 gcgaacgcat tctggcgtaa ggccgcgact ctagagtcga cctgcaggca tgcaagctga
     2881 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     2941 actagcataa ccccttgggg cggccgcttc gagcagacat gataagatac attgatgagt
     3001 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     3061 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     3121 ttcattttat gtttcaggtt cagggggaga tgtgggaggt ttttttaagc aagtaaaacc
     3181 tctacaaatg tggtaaaatc gaattttaac aaaatattaa cgcttacaat ttcctgatgc
     3241 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgcgga tctgcgcagc
     3301 accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa
     3361 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3421 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3481 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     3541 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3601 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3661 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttaattaact
     3721 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     3781 taaacccagg aggcagatca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     3841 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     3901 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     3961 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     4021 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     4081 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     4141 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     4201 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     4261 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     4321 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     4381 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     4441 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     4501 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     4561 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     4621 gaccaagcga cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt
     4681 acatctgtgt gttggttttt tgtgtgaatc gatagcgata aggatccgct catgcgatga
     4741 taagctgtca aacatgagaa ttacaactta tatcgtatgg ggctgacttc aggtgctaca
     4801 tttgaagaga taaattgcac tgaaatctag aaatatttta tctgattaat aagatgatct
     4861 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
     4921 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
     4981 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
     5041 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
     5101 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
     5161 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
     5221 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
     5281 caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
     5341 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
     5401 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
     5461 ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
     5521 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
     5581 caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
     5641 ccaacatagt aagccagtat acactccgct agcgctgatg tccggcggtg cttttgccgt
     5701 tacgcaccac cccgtcatga gattacgcgt tttagcggtg cgtacaatta agggattatg
     5761 gtaaatccac ttactgtctg ccctcgtagc catcgagata aaccgcagta ctccggccac
     5821 gatgcgtccg gcgtagagga tcgagatct
//
