LOCUS       Exported                6308 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-32Kp-PIK3C2B      9533 bp    DNA   circular          
             5/18/2017

            DEFINITION  Current Name: pF-32Kp - PIK3C2B
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     CDS             1065..2765
                     /label=DGKE sequence
     misc_feature    2766..2772
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    2773..2786
                     /label=C Terminal NanoLuc linker
     CDS             2787..3296
                     /label=C terminal NanoLuc
     polyA_signal    3433..3654
                     /label=Sv40 late polyadenylation signal
     promoter        3753..4171
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4179..4245
                     /label=EM7 bacterial promoter
     CDS             4259..5053
                     /label=Neo-Kan Resistance
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5117..5165
                     /label=Synthetic PolyAdenylation Signal
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggaa gcggagaggc
     1081 ggccggcgcc gggctcgccc tccgagggcc tgtttgcgga cgggcacctg atcttgtgga
     1141 cgctgtgctc ggtcctgctg ccggtgttca tcaccttctg gtgtagcctc cagcggtcgc
     1201 gccggcagct gcaccgcagg gacatcttcc gcaagagcaa gcacgggtgg cgcgacacgg
     1261 acctgttcag ccagcccacc tactgctgcg tgtgcgcgca gcacattctg cagggcgcct
     1321 tctgcgactg ctgcgggctc cgcgtggacg agggctgcct caggaaggcc gacaagcgct
     1381 tccagtgcaa ggagattatg ctcaagaatg acaccaaggt cctggacgcc atgccccacc
     1441 actggatccg gggcaacgtg cccctgtgca gttactgtat ggtttgcaag cagcagtgtg
     1501 gctgtcaacc caagctttgc gattacaggt gcatttggtg ccagaaaaca gtacatgatg
     1561 agtgcatgaa aaatagttta aagaatgaaa aatgtgattt tggagaattc aaaaacctaa
     1621 tcattccacc aagttattta acctccatta atcagatgcg taaagacaaa aaaacagatt
     1681 atgaagtgct agcctctaag cttggaaagc agtggacccc attaataatc ctggccaact
     1741 ctcgtagtgg aactaatatg ggagaaggac tgttgggaga atttaggatc ttgttgaatc
     1801 cagtccaggt ttttgatgta actaaaactc ctcctatcaa agccctacaa ctctgtactc
     1861 ttctcccata ttattcagct cgagtacttg tttgtggagg ggatgggact gtagggtggg
     1921 tcctggatgc agttgatgac atgaagatta agggacaaga aaagtacatt ccacaagttg
     1981 cagttttgcc tctgggaaca ggcaacgatc tatccaatac attgggttgg ggtacaggtt
     2041 atgctggaga aattccagtt gcgcaggttt tgcgaaatgt aatggaagca gatggaatta
     2101 aactagatcg atggaaagtt caagtaacaa ataaaggata ctacaactta agaaaaccca
     2161 aggaattcac aatgaacaac tatttttctg ttggacctga tgctctcatg gctctcaatt
     2221 ttcatgctca tcgtgagaag gcaccatctc tgttttctag cagaattctt aataaggcgg
     2281 tttacttatt ctatggaacc aaagattgtt tagtgcaaga atgtaaagat ttgaataaaa
     2341 aagttgagct agaactggat ggtgagcgag tagcactgcc cagcttggaa ggtattatag
     2401 ttctgaacat cggatactgg ggcggtggct gcagactatg ggaagggatg ggggacgaga
     2461 cttaccctct agccaggcat gacgatggtc tgctggaagt cgttggagta tatgggtctt
     2521 tccactgtgc tcagattcaa gtaaaactgg ctaatccttt tcgaatagga caggcacata
     2581 cagtgaggct gattttgaag tgctccatga tgccaatgca ggtggatggg gagccttggg
     2641 cccaagggcc ctgcactgtc accataactc acaagacaca tgcaatgatg ttatatttct
     2701 ctggagaaca aacagatgat gacatctcta gtacttcgga tcaagaagat ataaaggcga
     2761 ctgaagtttc tctcggctcg agcggcgtct tcacactcga agatttcgtt ggggactggc
     2821 gacagacagc cggctacaac ctggaccaag tccttgaaca gggaggtgtg tccagtttgt
     2881 ttcagaatct cggggtgtcc gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg
     2941 ggctgaagat cgacatccat gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg
     3001 gccagatcga aaaaattttt aaggtggtgt accctgtgga tgatcatcac tttaaggtga
     3061 tcctgcacta tggcacactg gtaatcgacg gggttacgcc gaacatgatc gactatttcg
     3121 gacggccgta tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta acagggaccc
     3181 tgtggaacgg caacaaaatt atcgacgagc gcctgatcaa ccccgacggc tccctgctgt
     3241 tccgagtaac catcaacgga gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaag
     3301 gccgcgactc tagagtcgac ctgcaggcat gcaagctgat ccggctgcta acaaagcccg
     3361 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     3421 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     3481 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3541 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3601 agggggagat gtgggaggtt tttttaagca agtaaaacct ctacaaatgt ggtaaaatcg
     3661 aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
     3721 tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca ccatggcctg aaataacctc
     3781 tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg
     3841 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3901 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3961 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4021 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4081 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4141 ttttggaggc ctaggctttt gcaaaaagct taattaactg ttgacaatta atcatcggca
     4201 tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcat
     4261 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     4321 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     4381 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     4441 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4501 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4561 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4621 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4681 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     4741 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     4801 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4861 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     4921 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     4981 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     5041 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     5101 ccatcacgat ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     5161 gtgtgaatcg atagcgataa ggatccgctc atgcgatgat aagctgtcaa acatgagaat
     5221 tacaacttat atcgtatggg gctgacttca ggtgctacat ttgaagagat aaattgcact
     5281 gaaatctaga aatattttat ctgattaata agatgatctt cttgagatcg ttttggtctg
     5341 cgcgtaatct cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt
     5401 ctctgagcta ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa
     5461 cttgtccttt cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca
     5521 attaccagtg gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg
     5581 atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag
     5641 cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc
     5701 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc
     5761 cgccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg
     5821 agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg
     5881 cggccctctc acttccctgt taagtatctt cctggcatct tccaggaaat ctccgccccg
     5941 ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga
     6001 ggaagcggaa tatatcctgt atcacatatt ctgctgacgc accggtgcag ccttttttct
     6061 cctgccacat gaagcacttc actgacaccc tcatcagtgc caacatagta agccagtata
     6121 cactccgcta gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcatgag
     6181 attacgcgtt ttagcggtgc gtacaattaa gggattatgg taaatccact tactgtctgc
     6241 cctcgtagcc atcgagataa accgcagtac tccggccacg atgcgtccgg cgtagaggat
     6301 cgagatct
//
