LOCUS       Exported                6448 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nn5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nn5K3
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1065..1577
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    1578..1589
                     /label=linker
                     /note="linker"
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     CDS             1599..3413
                     /label=PPM1D
     misc_feature    1599..2858
                     /label=Untitled
     misc_feature    3414..3421
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    3573..3794
                     /label=Sv40 late polyadenylation signal
     promoter        3893..4311
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4319..4385
                     /label=EM7 bacterial promoter
     CDS             4399..5193
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5257..5305
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    5348..6270
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    5525
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat ggcggggctg tactcgctgg
     1621 gagtgagcgt cttctccgac cagggcggga ggaagtacat ggaggacgtt actcaaatcg
     1681 ttgtggagcc cgaaccgacg gctgaagaaa agccctcgcc gcggcggtcg ctgtctcagc
     1741 cgttgcctcc gcggccgtcg ccggccgccc ttcccggcgg cgaagtctcg gggaaaggcc
     1801 cagcggtggc agcccgagag gctcgcgacc ctctcccgga cgccggggcc tcgccggcac
     1861 ctagccgctg ctgccgccgc cgttcctccg tggccttttt cgccgtgtgc gacgggcacg
     1921 gcgggcggga ggcggcacag tttgcccggg agcacttgtg gggtttcatc aagaagcaga
     1981 agggtttcac ctcgtccgag ccggctaagg tttgcgctgc catccgcaaa ggctttctcg
     2041 cttgtcacct tgccatgtgg aagaaactgg cggaatggcc aaagactatg acgggtcttc
     2101 ctagcacatc agggacaact gccagtgtgg tcatcattcg gggcatgaag atgtatgtag
     2161 ctcacgtagg tgactcaggg gtggttcttg gaattcagga tgacccgaag gatgactttg
     2221 tcagagctgt ggaggtgaca caggaccata agccagaact tcccaaggaa agagaacgaa
     2281 tcgaaggact tggtgggagt gtaatgaaca agtctggggt gaatcgtgta gtttggaaac
     2341 gacctcgact cactcacaat ggacctgtta gaaggagcac agttattgac cagattcctt
     2401 ttctggcagt agcaagagca cttggtgatt tgtggagcta tgatttcttc agtggtgaat
     2461 ttgtggtgtc acctgaacca gacacaagtg tccacactct tgaccctcag aagcacaagt
     2521 atattatatt ggggagtgat ggactttgga atatgattcc accacaagat gccatctcaa
     2581 tgtgccagga ccaagaggag aaaaaatacc tgatgggtga gcatggacaa tcttgtgcca
     2641 aaatgcttgt gaatcgagca ttgggccgct ggaggcagcg tatgctccga gcagataaca
     2701 ctagtgccat agtaatctgc atctctccag aagtggacaa tcagggaaac tttaccaatg
     2761 aagatgagtt atacctgaac ctgactgaca gcccttccta taatagtcaa gaaacctgtg
     2821 tgatgactcc ttccccatgt tctacaccac cagtcaagtc actggaggag gatccatggc
     2881 caagggtgaa ttctaaggac catatacctg ccctggttcg tagcaatgcc ttctcagaga
     2941 attttttaga ggtttcagct gagatagctc gagagaatgt ccaaggtgta gtcataccct
     3001 caaaagatcc agaaccactt gaagaaaatt gcgctaaagc cctgacttta aggatacatg
     3061 attctttgaa taatagcctt ccaattggcc ttgtgcctac taattcaaca aacactgtca
     3121 tggaccaaaa aaatttgaag atgtcaactc ctggccaaat gaaagcccaa gaaattgaaa
     3181 gaacccctcc aacaaacttt aaaaggacat tagaagagtc caattctggc cccctgatga
     3241 agaagcatag acgaaatggc ttaagtcgaa gtagtggtgc tcagcctgca agtctcccca
     3301 caacctcaca gcgaaagaac tctgttaaac tcaccatgcg acgcagactt aggggccaga
     3361 agaaaattgg aaatccttta cttcatcaac acaggaaaac tgtttgtgtt tgcgtttaaa
     3421 cgaattcggg ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagctgat
     3481 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     3541 ctagcataac cccttggggc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     3601 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     3661 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     3721 tcattttatg tttcaggttc agggggagat gtgggaggtt tttttaagca agtaaaacct
     3781 ctacaaatgt ggtaaaatcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
     3841 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca
     3901 ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag
     3961 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4021 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4081 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4141 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4201 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     4261 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct taattaactg
     4321 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     4381 aaacccagga ggcagatcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     4441 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4501 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     4561 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     4621 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     4681 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     4741 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     4801 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     4861 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     4921 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     4981 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5041 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5101 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5161 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     5221 accaagcgac gcccaacctg ccatcacgat ggccgcaata aaatatcttt attttcatta
     5281 catctgtgtg ttggtttttt gtgtgaatcg atagcgataa ggatccgctc atgcgatgat
     5341 aagctgtcaa acatgagaat tacaacttat atcgtatggg gctgacttca ggtgctacat
     5401 ttgaagagat aaattgcact gaaatctaga aatattttat ctgattaata agatgatctt
     5461 cttgagatcg ttttggtctg cgcgtaatct cttgctctga aaacgaaaaa accgccttgc
     5521 agggcggttt ttcgaaggtt ctctgagcta ccaactcttt gaaccgaggt aactggcttg
     5581 gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc ttaaccggcg catgacttca
     5641 agactaactc ctctaaatca attaccagtg gctgctgcca gtggtgcttt tgcatgtctt
     5701 tccgggttgg actcaagacg atagttaccg gataaggcgc agcggtcgga ctgaacgggg
     5761 ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag tgtcaggcgt
     5821 ggaatgagac aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac
     5881 aggagagcgc acgagggagc cgccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5941 gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct
     6001 atggaaaaac ggctttgccg cggccctctc acttccctgt taagtatctt cctggcatct
     6061 tccaggaaat ctccgccccg ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag
     6121 cgtagcgagt cagtgagcga ggaagcggaa tatatcctgt atcacatatt ctgctgacgc
     6181 accggtgcag ccttttttct cctgccacat gaagcacttc actgacaccc tcatcagtgc
     6241 caacatagta agccagtata cactccgcta gcgctgatgt ccggcggtgc ttttgccgtt
     6301 acgcaccacc ccgtcatgag attacgcgtt ttagcggtgc gtacaattaa gggattatgg
     6361 taaatccact tactgtctgc cctcgtagcc atcgagataa accgcagtac tccggccacg
     6421 atgcgtccgg cgtagaggat cgagatct
//
