LOCUS       Exported                5350 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nn5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nn5K3
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1065..1577
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    1578..1589
                     /label=linker
                     /note="linker"
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1599..2315
                     /label=BCL2
                     /note="Recieved from Kazusa in Nov 2009. Entered into 
                     database semi automatically by Ethan in Jan 2010. (note 
                     added in Ethan Strauss by 2/1/2010 10:15:22 AM"
     misc_feature    2316..2323
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    2475..2696
                     /label=Sv40 late polyadenylation signal
     promoter        2795..3213
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        3221..3287
                     /label=EM7 bacterial promoter
     CDS             3301..4095
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4159..4207
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    4250..5172
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    4427
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat ggcgcacgct gggagaacag
     1621 ggtacgataa ccgggagata gtgatgaagt acatccatta taagctgtcg cagaggggct
     1681 acgagtggga tgcgggagat gtgggcgccg cgcccccggg ggccgccccc gcaccgggca
     1741 tcttctcctc ccagcccggg cacacgcccc atccagccgc atcccgggac ccggtcgcca
     1801 ggacctcgcc gctgcagacc ccggctgccc ccggcgccgc cgcggggcct gcgctcagcc
     1861 cggtgccacc tgtggtccac ctgaccctcc gccaggccgg cgacgacttc tcccgccgct
     1921 accgccgcga cttcgccgag atgtccagcc agctgcacct gacgcccttc accgcgcggg
     1981 gacgctttgc cacggtggtg gaggagctct tcagggacgg ggtgaactgg gggaggattg
     2041 tggccttctt tgagttcggt ggggtcatgt gtgtggagag cgtcaaccgg gagatgtcgc
     2101 ccctggtgga caacatcgcc ctgtggatga ctgagtacct gaaccggcac ctgcacacct
     2161 ggatccagga taacggaggc tgggatgcct ttgtggaact gtacggcccc agcatgcggc
     2221 ctctgtttga tttctcctgg ctgtctctga agactctgct cagtttggcc ctggtgggag
     2281 cttgcatcac cctgggtgcc tatctgggcc acaaggttta aacgaattcg ggctcggtac
     2341 ccggggatcc tctagagtcg acctgcaggc atgcaagctg atccggctgc taacaaagcc
     2401 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     2461 gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     2521 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     2581 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2641 tcagggggag atgtgggagg tttttttaag caagtaaaac ctctacaaat gtggtaaaat
     2701 cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     2761 tctgtgcggt atttcacacc gcatacgcgg atctgcgcag caccatggcc tgaaataacc
     2821 tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct gtggaatgtg
     2881 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2941 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     3001 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     3061 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3121 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     3181 ttttttggag gcctaggctt ttgcaaaaag cttaattaac tgttgacaat taatcatcgg
     3241 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaacccag gaggcagatc
     3301 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     3361 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     3421 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     3481 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     3541 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     3601 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     3661 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     3721 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     3781 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     3841 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     3901 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     3961 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     4021 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     4081 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     4141 tgccatcacg atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     4201 ttgtgtgaat cgatagcgat aaggatccgc tcatgcgatg ataagctgtc aaacatgaga
     4261 attacaactt atatcgtatg gggctgactt caggtgctac atttgaagag ataaattgca
     4321 ctgaaatcta gaaatatttt atctgattaa taagatgatc ttcttgagat cgttttggtc
     4381 tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg
     4441 ttctctgagc taccaactct ttgaaccgag gtaactggct tggaggagcg cagtcaccaa
     4501 aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac tcctctaaat
     4561 caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt ggactcaaga
     4621 cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc
     4681 agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg
     4741 ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga
     4801 gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt
     4861 tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggctttgc
     4921 cgcggccctc tcacttccct gttaagtatc ttcctggcat cttccaggaa atctccgccc
     4981 cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc
     5041 gaggaagcgg aatatatcct gtatcacata ttctgctgac gcaccggtgc agcctttttt
     5101 ctcctgccac atgaagcact tcactgacac cctcatcagt gccaacatag taagccagta
     5161 tacactccgc tagcgctgat gtccggcggt gcttttgccg ttacgcacca ccccgtcatg
     5221 agattacgcg ttttagcggt gcgtacaatt aagggattat ggtaaatcca cttactgtct
     5281 gccctcgtag ccatcgagat aaaccgcagt actccggcca cgatgcgtcc ggcgtagagg
     5341 atcgagatct
//
