LOCUS       Exported                5324 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nc5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nc5K3
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..1781
                     /label=BCL2
                     /note="Recieved from Kazusa in Nov 2009. Entered into 
                     database semi automatically by Ethan in Jan 2010. (note 
                     added in Ethan Strauss by 2/1/2010 10:15:22 AM"
     misc_feature    1788..1802
                     /label=linker
                     /note="linker"
     misc_feature    1803..2312
                     /label=NanoLuc
                     /note="NanoLuc"
     polyA_signal    2449..2670
                     /label=Sv40 late polyadenylation signal
     promoter        2769..3187
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        3195..3261
                     /label=EM7 bacterial promoter
     CDS             3275..4069
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4133..4181
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    4224..5146
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    4401
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggcg cacgctggga
     1081 gaacagggta cgataaccgg gagatagtga tgaagtacat ccattataag ctgtcgcaga
     1141 ggggctacga gtgggatgcg ggagatgtgg gcgccgcgcc cccgggggcc gcccccgcac
     1201 cgggcatctt ctcctcccag cccgggcaca cgccccatcc agccgcatcc cgggacccgg
     1261 tcgccaggac ctcgccgctg cagaccccgg ctgcccccgg cgccgccgcg gggcctgcgc
     1321 tcagcccggt gccacctgtg gtccacctga ccctccgcca ggccggcgac gacttctccc
     1381 gccgctaccg ccgcgacttc gccgagatgt ccagccagct gcacctgacg cccttcaccg
     1441 cgcggggacg ctttgccacg gtggtggagg agctcttcag ggacggggtg aactggggga
     1501 ggattgtggc cttctttgag ttcggtgggg tcatgtgtgt ggagagcgtc aaccgggaga
     1561 tgtcgcccct ggtggacaac atcgccctgt ggatgactga gtacctgaac cggcacctgc
     1621 acacctggat ccaggataac ggaggctggg atgcctttgt ggaactgtac ggccccagca
     1681 tgcggcctct gtttgatttc tcctggctgt ctctgaagac tctgctcagt ttggccctgg
     1741 tgggagcttg catcaccctg ggtgcctatc tgggccacaa ggtttctctc ggctcgagcg
     1801 gcgtcttcac actcgaagat ttcgttgggg actggcgaca gacagccggc tacaacctgg
     1861 accaagtcct tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa
     1921 ctccgatcca aaggattgtc ctgagcggtg aaaatgggct gaagatcgac atccatgtca
     1981 tcatcccgta tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa atttttaagg
     2041 tggtgtaccc tgtggatgat catcacttta aggtgatcct gcactatggc acactggtaa
     2101 tcgacggggt tacgccgaac atgatcgact atttcggacg gccgtatgaa ggcatcgccg
     2161 tgttcgacgg caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg
     2221 acgagcgcct gatcaacccc gacggctccc tgctgttccg agtaaccatc aacggagtga
     2281 ccggctggcg gctgtgcgaa cgcattctgg cgtaaggccg cgactctaga gtcgacctgc
     2341 aggcatgcaa gctgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     2401 caccgctgag caataactag cataacccct tggggcggcc gcttcgagca gacatgataa
     2461 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     2521 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     2581 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
     2641 taagcaagta aaacctctac aaatgtggta aaatcgaatt ttaacaaaat attaacgctt
     2701 acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     2761 gcggatctgc gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac
     2821 cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     2881 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     2941 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3001 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3061 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3121 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3181 aaagcttaat taactgttga caattaatca tcggcatagt atatcggcat agtataatac
     3241 gacaaggtga ggaactaaac ccaggaggca gatcatgatt gaacaagatg gattgcacgc
     3301 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     3361 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     3421 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     3481 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     3541 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     3601 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     3661 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     3721 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     3781 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     3841 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     3901 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     3961 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     4021 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     4081 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgatggcc gcaataaaat
     4141 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag cgataaggat
     4201 ccgctcatgc gatgataagc tgtcaaacat gagaattaca acttatatcg tatggggctg
     4261 acttcaggtg ctacatttga agagataaat tgcactgaaa tctagaaata ttttatctga
     4321 ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac
     4381 gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac
     4441 cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa
     4501 ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg
     4561 tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4621 gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga
     4681 actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa
     4741 accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc
     4801 tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt
     4861 caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag
     4921 tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg
     4981 cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca
     5041 catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg
     5101 acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgatgtccgg
     5161 cggtgctttt gccgttacgc accaccccgt catgagatta cgcgttttag cggtgcgtac
     5221 aattaaggga ttatggtaaa tccacttact gtctgccctc gtagccatcg agataaaccg
     5281 cagtactccg gccacgatgc gtccggcgta gaggatcgag atct
//
