LOCUS       Exported                5306 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nc5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nc5K3
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..1763
                     /label=BCL2L1
                     /note="Created by Kazusa. Available from Kazusa. Data 
                     recieved from Kazusa 11/2008. Entered by Ethan 12/2008.  
                     End definitions corrected 5/2010. Name changed from 
                     KIBB8901 to BCL2L1 by Ethan 5/17/2010. (note added in 
                     estrauss by 5/17/2010 3:20:48 PM"
     misc_feature    1770..1784
                     /label=linker
                     /note="linker"
     misc_feature    1785..2294
                     /label=NanoLuc
                     /note="NanoLuc"
     polyA_signal    2431..2652
                     /label=Sv40 late polyadenylation signal
     promoter        2751..3169
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        3177..3243
                     /label=EM7 bacterial promoter
     CDS             3257..4051
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4115..4163
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    4206..5128
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    4383
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgtct cagagcaacc
     1081 gggagctggt ggttgacttt ctctcctaca agctttccca gaaaggatac agctggagtc
     1141 agtttagtga tgtggaagag aacaggactg aggccccaga agggactgaa tcggagatgg
     1201 agacccccag tgccatcaat ggcaacccat cctggcacct ggcagacagc cccgcggtga
     1261 atggagccac tggccacagc agcagtttgg atgcccggga ggtgatcccc atggcagcag
     1321 taaagcaagc gctgagggag gcaggcgacg agtttgaact gcggtaccgg cgggcattca
     1381 gtgacctgac atcccagctc cacatcaccc cagggacagc atatcagagc tttgaacagg
     1441 tagtgaatga actcttccgg gatggggtaa actggggtcg cattgtggcc tttttctcct
     1501 tcggcggggc actgtgcgtg gaaagcgtag acaaggagat gcaggtattg gtgagtcgga
     1561 tcgcagcttg gatggccact tacctgaatg accacctaga gccttggatc caggagaacg
     1621 gcggctggga tacttttgtg gaactctatg ggaacaatgc agcagccgag agccgaaagg
     1681 gccaggaacg cttcaaccgc tggttcctga cgggcatgac tgtggccggc gtggttctgc
     1741 tgggctcact cttcagtcgg aaagtttctc tcggctcgag cggcgtcttc acactcgaag
     1801 atttcgttgg ggactggcga cagacagccg gctacaacct ggaccaagtc cttgaacagg
     1861 gaggtgtgtc cagtttgttt cagaatctcg gggtgtccgt aactccgatc caaaggattg
     1921 tcctgagcgg tgaaaatggg ctgaagatcg acatccatgt catcatcccg tatgaaggtc
     1981 tgagcggcga ccaaatgggc cagatcgaaa aaatttttaa ggtggtgtac cctgtggatg
     2041 atcatcactt taaggtgatc ctgcactatg gcacactggt aatcgacggg gttacgccga
     2101 acatgatcga ctatttcgga cggccgtatg aaggcatcgc cgtgttcgac ggcaaaaaga
     2161 tcactgtaac agggaccctg tggaacggca acaaaattat cgacgagcgc ctgatcaacc
     2221 ccgacggctc cctgctgttc cgagtaacca tcaacggagt gaccggctgg cggctgtgcg
     2281 aacgcattct ggcgtaaggc cgcgactcta gagtcgacct gcaggcatgc aagctgatcc
     2341 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     2401 agcataaccc cttggggcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     2461 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     2521 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     2581 attttatgtt tcaggttcag ggggagatgt gggaggtttt tttaagcaag taaaacctct
     2641 acaaatgtgg taaaatcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt
     2701 attttctcct tacgcatctg tgcggtattt cacaccgcat acgcggatct gcgcagcacc
     2761 atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa
     2821 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     2881 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     2941 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     3001 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3061 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3121 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta attaactgtt
     3181 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     3241 acccaggagg cagatcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3301 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     3361 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     3421 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     3481 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     3541 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     3601 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     3661 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     3721 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     3781 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     3841 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     3901 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     3961 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4021 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     4081 caagcgacgc ccaacctgcc atcacgatgg ccgcaataaa atatctttat tttcattaca
     4141 tctgtgtgtt ggttttttgt gtgaatcgat agcgataagg atccgctcat gcgatgataa
     4201 gctgtcaaac atgagaatta caacttatat cgtatggggc tgacttcagg tgctacattt
     4261 gaagagataa attgcactga aatctagaaa tattttatct gattaataag atgatcttct
     4321 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag
     4381 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga
     4441 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag
     4501 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc
     4561 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg
     4621 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
     4681 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
     4741 gagagcgcac gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4801 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat
     4861 ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc
     4921 caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg
     4981 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac
     5041 cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca
     5101 acatagtaag ccagtataca ctccgctagc gctgatgtcc ggcggtgctt ttgccgttac
     5161 gcaccacccc gtcatgagat tacgcgtttt agcggtgcgt acaattaagg gattatggta
     5221 aatccactta ctgtctgccc tcgtagccat cgagataaac cgcagtactc cggccacgat
     5281 gcgtccggcg tagaggatcg agatct
//
