LOCUS       Exported                5332 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nn5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nn5K3
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1065..1577
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    1578..1589
                     /label=linker
                     /note="linker"
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1599..2297
                     /label=BCL2L1
                     /note="Created by Kazusa. Available from Kazusa. Data 
                     recieved from Kazusa 11/2008. Entered by Ethan 12/2008.  
                     End definitions corrected 5/2010. Name changed from 
                     KIBB8901 to BCL2L1 by Ethan 5/17/2010. (note added in 
                     estrauss by 5/17/2010 3:20:48 PM"
     misc_feature    2298..2305
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    2457..2678
                     /label=Sv40 late polyadenylation signal
     promoter        2777..3195
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        3203..3269
                     /label=EM7 bacterial promoter
     CDS             3283..4077
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4141..4189
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    4232..5154
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    4409
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat gtctcagagc aaccgggagc
     1621 tggtggttga ctttctctcc tacaagcttt cccagaaagg atacagctgg agtcagttta
     1681 gtgatgtgga agagaacagg actgaggccc cagaagggac tgaatcggag atggagaccc
     1741 ccagtgccat caatggcaac ccatcctggc acctggcaga cagccccgcg gtgaatggag
     1801 ccactggcca cagcagcagt ttggatgccc gggaggtgat ccccatggca gcagtaaagc
     1861 aagcgctgag ggaggcaggc gacgagtttg aactgcggta ccggcgggca ttcagtgacc
     1921 tgacatccca gctccacatc accccaggga cagcatatca gagctttgaa caggtagtga
     1981 atgaactctt ccgggatggg gtaaactggg gtcgcattgt ggcctttttc tccttcggcg
     2041 gggcactgtg cgtggaaagc gtagacaagg agatgcaggt attggtgagt cggatcgcag
     2101 cttggatggc cacttacctg aatgaccacc tagagccttg gatccaggag aacggcggct
     2161 gggatacttt tgtggaactc tatgggaaca atgcagcagc cgagagccga aagggccagg
     2221 aacgcttcaa ccgctggttc ctgacgggca tgactgtggc cggcgtggtt ctgctgggct
     2281 cactcttcag tcggaaagtt taaacgaatt cgggctcggt acccggggat cctctagagt
     2341 cgacctgcag gcatgcaagc tgatccggct gctaacaaag cccgaaagga agctgagttg
     2401 gctgctgcca ccgctgagca ataactagca taaccccttg gggcggccgc ttcgagcaga
     2461 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2521 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2581 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
     2641 ggttttttta agcaagtaaa acctctacaa atgtggtaaa atcgaatttt aacaaaatat
     2701 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     2761 ccgcatacgc ggatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg
     2821 ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     2881 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2941 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3001 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3061 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3121 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3181 ttttgcaaaa agcttaatta actgttgaca attaatcatc ggcatagtat atcggcatag
     3241 tataatacga caaggtgagg aactaaaccc aggaggcaga tcatgattga acaagatgga
     3301 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     3361 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     3421 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     3481 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     3541 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     3601 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     3661 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     3721 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     3781 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     3841 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     3901 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     3961 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     4021 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     4081 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgatggccgc
     4141 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagcg
     4201 ataaggatcc gctcatgcga tgataagctg tcaaacatga gaattacaac ttatatcgta
     4261 tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc tagaaatatt
     4321 ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta atctcttgct
     4381 ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga gctaccaact
     4441 ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc ctttcagttt
     4501 agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc agtggctgct
     4561 gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt accggataag
     4621 gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc
     4681 tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac
     4741 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag ggggaaacgc
     4801 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg
     4861 atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc tctcacttcc
     4921 ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta agccatttcc
     4981 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc
     5041 ctgtatcaca tattctgctg acgcaccggt gcagcctttt ttctcctgcc acatgaagca
     5101 cttcactgac accctcatca gtgccaacat agtaagccag tatacactcc gctagcgctg
     5161 atgtccggcg gtgcttttgc cgttacgcac caccccgtca tgagattacg cgttttagcg
     5221 gtgcgtacaa ttaagggatt atggtaaatc cacttactgt ctgccctcgt agccatcgag
     5281 ataaaccgca gtactccggc cacgatgcgt ccggcgtaga ggatcgagat ct
//
