LOCUS       Exported                6578 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nn5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nn5K3
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1065..1577
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    1578..1589
                     /label=linker
                     /note="linker"
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1599..3407
                     /label=PLK1-31K
                     /note="PLK1-31K"
     misc_feature    3408..3415
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    3567..3788
                     /label=Sv40 late polyadenylation signal
     promoter        3887..4305
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4313..4379
                     /label=EM7 bacterial promoter
     CDS             4393..5187
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5251..5299
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      5535..5571
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat gagtgctgca gtgactgcag
     1621 ggaagctggc acgggcaccg gccgaccctg ggaaagccgg ggtccccgga gttgcagctc
     1681 ccggagctcc ggcggcggct ccaccggcga aagagatccc ggaggtccta gtggacccac
     1741 gcagccggcg gcgctatgtg cggggccgct ttttgggcaa gggcggcttt gccaagtgct
     1801 tcgagatctc ggacgcggac accaaggagg tgttcgcggg caagattgtg cctaagtctc
     1861 tgctgctcaa gccgcaccag agggagaaga tgtccatgga aatatccatt caccgcagcc
     1921 tcgcccacca gcacgtcgta ggattccacg gctttttcga ggacaacgac ttcgtgttcg
     1981 tggtgttgga gctctgccgc cggaggtctc tcctggagct gcacaagagg aggaaagccc
     2041 tgactgagcc tgaggcccga tactacctac ggcaaattgt gcttggctgc cagtacctgc
     2101 accgaaaccg agttattcat cgagacctca agctgggcaa ccttttcctg aatgaagatc
     2161 tggaggtgaa aataggggat tttggactgg caaccaaagt cgaatatgac ggggagagga
     2221 agaagaccct gtgtgggact cctaattaca tagctcccga ggtgctgagc aagaaagggc
     2281 acagtttcga ggtggatgtg tggtccattg ggtgtatcat gtataccttg ttagtgggca
     2341 aaccaccttt tgagacttct tgcctaaaag agacctacct ccggatcaag aagaatgaat
     2401 acagtattcc caagcacatc aaccccgtgg ccgcctccct catccagaag atgcttcaga
     2461 cagatcccac tgcccgccca accattaacg agctgcttaa tgacgagttc tttacttctg
     2521 gctatatccc tgcccgtctc cccatcacct gcctgaccat tccaccaagg ttttcgattg
     2581 ctcccagcag cctggacccc agcaaccgga agcccctcac agtcctcaat aaaggcttgg
     2641 agaaccccct gcctgagcgt ccccgggaaa aagaagaacc agtggttcga gagacaggtg
     2701 aggtggtcga ctgccacctc agtgacatgc tgcagcagct gcacagtgtc aatgcctcca
     2761 agccctcgga gcgtgggctg gtcaggcaag aggaggctga ggatcctgcc tgcatcccca
     2821 tcttctgggt cagcaagtgg gtggactatt cggacaagta cggccttggg tatcagctct
     2881 gtgataacag cgtgggggtg ctcttcaatg actcaacacg cctcatcctc tacaatgatg
     2941 gtgacagcct gcagtacata gagcgtgacg gcactgagtc ctacctcacc gtgagttccc
     3001 atcccaactc cttgatgaag aagatcaccc tccttaaata tttccgcaat tacatgagcg
     3061 agcacttgct gaaggcaggt gccaacatca cgccgcgcga aggtgatgag ctcgcccggc
     3121 tgccctacct acggacctgg ttccgcaccc gcagcgccat catcctgcac ctcagcaacg
     3181 gcagcgtgca gatcaacttc ttccaggatc acaccaagct catcttgtgc ccactgatgg
     3241 cagccgtgac ctacatcgac gagaagcggg acttccgcac ataccgcctg agtctcctgg
     3301 aggagtacgg ctgctgcaag gagctggcca gccggctccg ctacgcccgc actatggtgg
     3361 acaagctgct gagctcacgc tcggccagca accgtctcaa ggcctccgtt taaacgaatt
     3421 cgggctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc tgatccggct
     3481 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     3541 taaccccttg gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     3601 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     3661 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     3721 tatgtttcag gttcaggggg agatgtggga ggttttttta agcaagtaaa acctctacaa
     3781 atgtggtaaa atcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt
     3841 tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg
     3901 cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg aaagaaccag
     3961 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4021 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4081 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4141 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4201 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     4261 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttaatta actgttgaca
     4321 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaaccc
     4381 aggaggcaga tcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4441 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4501 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4561 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4621 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4681 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     4741 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4801 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4861 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4921 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     4981 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     5041 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     5101 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     5161 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     5221 cgacgcccaa cctgccatca cgatggccgc aataaaatat ctttattttc attacatctg
     5281 tgtgttggtt ttttgtgtga atcgatagcg ataaggatcc tctttgcgct tgcgttttcc
     5341 cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg
     5401 gctttctacc cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5461 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5521 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5581 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5641 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5701 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5761 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5821 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5881 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5941 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     6001 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6061 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6121 tttcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6181 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttatag
     6241 tccggaaata caggaacgca cgctggatgg cccttcgctg ggatggtgaa accatgaaaa
     6301 atggcagctt cagtggatta agtgggggta atgtggcctg taccctctgg ttgcataggt
     6361 attcatacgg ttaaaattta tcaggcgcga ttgcggcagt ttttcgggtg gtttgttgcc
     6421 atttttacct gtctgctgcc gtgatcgcgc tgaacgcgtt ttagcggtgc gtacaattaa
     6481 gggattatgg taaatccact tactgtctgc cctcgtagcc atcgagataa accgcagtac
     6541 tccggccacg atgcgtccgg cgtagaggat cgagatct
//
