LOCUS       BIRC3-Nn5K2             6581 bp ds-DNA     circular     01-MAY-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            ABL1(H396P)-Nn5K2.gb Current Name: pF-Nn5K2 - ABL1 Sequence
            'ABL1(H396P)-Nn5K2' generated by SeqNinja on Jun 22, 2022 at
            8:15:56 AM CDT. Equivalent SeqNinja expression: "G:\Public\1810DFK
            Investigation\Plasmids to Upload to Benchling\A
            Mutants\ABL1(H396P)-Nn5K2.sbd"#"ABL1(H396P)-Nn5K2"
FEATURES             Location/Qualifiers
     promoter        1..742
                     /label="CMV immediate-early enhancer/promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="note: Has EcoICRI site removed. Named changed from """"CMVPromoter"""" to """"CMV immediate-early enhancer/promoter""""3/21/2011 and J Hartnet's request to fit Sci Com'snaming. dnas_type: CMV immediate-early enhancer/promoter"
     intron          857..989
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="note: Note that this is the actual sequence spliced out.The 5' donor site includes a 5' GA and the 3' acceptorsite adds a 3'G. dnas_type: chimeric intron"
     promoter        1033..1052
                     /label="T7 RNA Polymerase promoter (-17 to +3)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="dnas_type: T7 RNA Polymerase promoter"
     CDS             1065..1577
                     /label="NanoLuc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="dnas_type: NanoLuc"
     CDS             1065..1577
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILA"
     misc_feature    1578..1589
                     /label="N Terminal NanoLuc linker"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="dnas_type: N Terminal NanoLuc linker"
     misc_feature    1590..1597
                     /label="SgfI Site"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="note: Used in Flexi Vector Cloning dnas_type: SgfI Site"
     CDS             1599..3410
                     /label="BIRC3 sequence"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="dnas_type: BIRC3 sequence"
     CDS             1599..3410
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MNIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSVNNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRSSYHCAMNNENARLLTFQTWPLTFLSPTNLAKAGFYYIGPGDRVACFACGGKLSNWEPKDNAMSEHLRHFPKCPFIENQLQDTSRYTVSNLSMQTHAARFKTFFNWPSSVLVNPEQLASAGFYYVGNSDDVKCFCCDGGLRCWESGDDPWVQHAKWFPRCEYLIRIKGQEFIRQVQASYPHLLEQLLSTSDSPGDENAESSIIHFEPGEDHSEDAIMMNTPVINAAVEMGFSRSLVKQTVQRKILATGENYRLVNDLVLDLLNAEDEIREEERERATEEKESNDLLLIRKNRMALFQHLTCVIPILDSLLTAGIINEQEHDVIKQKTQTSLQARELIDTILVKGNIAATVFRNSLQEAEAVLYEHLFVQQDIKYIPTEDVSDLPVEEQLRRLQEERTCKVCMDKEVSIVFIPCGHLVVCKDCAPSLRKCPICRSTIKGTVRTFLS"
     misc_feature    3411..3418
                     /label="PmeI site"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="note: Used in Flexi Vector Cloning dnas_type: PmeI site"
     misc_feature    3415..3548
                     /label="Transfer Efficiency Cassette"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="note: Feature changed from""""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGC"""" at Jim Hartnet's request12/18/2007 to include the complete cassette. dnas_type: Transfer Efficiency Cassette"
     polyA_signal    3570..3791
                     /label="Sv40 late polyadenylation signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="dnas_type: Sv40 late polyadenylation signal"
     promoter        3890..4308
                     /label="SV40 enhancer and early promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="note: Name changed frm """"SV40 early enhancer/promoterregion"""" to """"SV40 enhancer and early promoter"""" on3/21/2011 at Jim Hartnet's request to fit Sci Com'snaming. dnas_type: SV40 enhancer and early promoter"
     enhancer        complement(3963..4199)
                     /label="SV40 Enhancer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="note: Feature was found in the reverse orientation. dnas_type: SV40 Enhancer"
     rep_origin      4206..4271
                     /label="Sv40 Min Ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="dnas_type: Sv40 Min Ori"
     promoter        4316..4382
                     /label="EM7 bacterial promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="dnas_type: EM7 bacterial promoter"
     CDS             4396..5190
                     /label="Neomycin phosphotransferase coding region"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: Name changed frm """"Neomycin phosphotransferase""""to """"Neomycin phosphotransferase coding region"""" on3/21/2011 at Jim Hartnet's request to fit Sci Com'snaming. dnas_type: Neomycin phosphotransferase coding"
     CDS             4396..5190
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     polyA_signal    5254..5302
                     /label="Synthetic PolyAdenylation Signal Sequence (frompCiNeo vectors)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="dnas_type: Synthetic PolyAdenylation Signal"
     rep_origin      5538..5574
                     /label="ColE1-derived plasmid replication origin"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="note: The actual origin is the first base. dnas_type: ColE1-derived plasmid replication origin"
     misc_feature    6245..6530
                     /label="Cer Region"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="note: Prevents multimerization dnas_type: Cer Region"
ORIGIN
        1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA ATATTGGCTA
       61 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC
      121 AATATGACCG CCATGTTGGC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG
      181 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC
      241 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
      301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC
      361 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA
      421 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG
      481 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC
      541 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT
      601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAATAACCC
      661 CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC
      721 TGGTTTAGTG AACCGTCAGA TCACTAGAAG CTTTATTGCG GTAGTTTATC ACAGTTAAAT
      781 TGCTAACGCA GTCAGTGCTT CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC
      841 GTGAGGCACT GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA
      901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA TTGGTCTTAC
      961 TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC CAGTTCAATT ACAGCTCTTA
     1021 AGGCTAGAGT ATTAATACGA CTCACTATAG GGCTAGCGCT CACCATGGTC TTCACACTCG
     1081 AAGATTTCGT TGGGGACTGG CGACAGACAG CCGGCTACAA CCTGGACCAA GTCCTTGAAC
     1141 AGGGAGGTGT GTCCAGTTTG TTTCAGAATC TCGGGGTGTC CGTAACTCCG ATCCAAAGGA
     1201 TTGTCCTGAG CGGTGAAAAT GGGCTGAAGA TCGACATCCA TGTCATCATC CCGTATGAAG
     1261 GTCTGAGCGG CGACCAAATG GGCCAGATCG AAAAAATTTT TAAGGTGGTG TACCCTGTGG
     1321 ATGATCATCA CTTTAAGGTG ATCCTGCACT ATGGCACACT GGTAATCGAC GGGGTTACGC
     1381 CGAACATGAT CGACTATTTC GGACGGCCGT ATGAAGGCAT CGCCGTGTTC GACGGCAAAA
     1441 AGATCACTGT AACAGGGACC CTGTGGAACG GCAACAAAAT TATCGACGAG CGCCTGATCA
     1501 ACCCCGACGG CTCCCTGCTG TTCCGAGTAA CCATCAACGG AGTGACCGGC TGGCGGCTGT
     1561 GCGAACGCAT TCTGGCGGGC TCGAGCGGCG CGATCGCCAT GAACATAGTA GAAAACAGCA
     1621 TATTCTTATC AAATTTGATG AAAAGCGCCA ACACGTTTGA ACTGAAATAC GACTTGTCAT
     1681 GTGAACTGTA CCGAATGTCT ACGTATTCCA CTTTTCCTGC TGGGGTTCCT GTCTCAGAAA
     1741 GGAGTCTTGC TCGTGCTGGT TTCTATTACA CTGGTGTGAA TGACAAGGTC AAATGCTTCT
     1801 GTTGTGGCCT GATGCTGGAT AACTGGAAAA GAGGAGACAG TCCTACTGAA AAGCATAAAA
     1861 AGTTGTATCC TAGCTGCAGA TTCGTTCAGA GTCTAAATTC CGTTAACAAC TTGGAAGCTA
     1921 CCTCTCAGCC TACTTTTCCT TCTTCAGTAA CAAATTCCAC ACACTCATTA CTTCCGGGTA
     1981 CAGAAAACAG TGGATATTTC CGTGGCTCTT ATTCAAACTC TCCATCAAAT CCTGTAAACT
     2041 CCAGAGCAAA TCAAGATTTT TCTGCCTTGA TGAGAAGTTC CTACCACTGT GCAATGAATA
     2101 ACGAAAATGC CAGATTACTT ACTTTTCAGA CATGGCCATT GACTTTTCTG TCGCCAACAA
     2161 ATCTGGCAAA AGCAGGCTTT TACTACATAG GACCTGGAGA CAGAGTGGCT TGCTTTGCCT
     2221 GTGGTGGAAA ATTGAGCAAT TGGGAACCGA AGGATAATGC TATGTCAGAA CACCTGAGAC
     2281 ATTTTCCCAA ATGCCCATTT ATAGAAAATC AGCTTCAAGA CACTTCAAGA TACACAGTTT
     2341 CTAATCTGAG CATGCAGACA CATGCAGCCC GCTTTAAAAC ATTCTTTAAC TGGCCCTCTA
     2401 GTGTTCTAGT TAATCCTGAG CAGCTTGCAA GTGCGGGTTT TTATTATGTG GGTAACAGTG
     2461 ATGATGTCAA ATGCTTTTGC TGTGATGGTG GACTCAGGTG TTGGGAATCT GGAGATGATC
     2521 CATGGGTTCA ACATGCCAAG TGGTTTCCAA GGTGTGAGTA CTTGATAAGA ATTAAAGGAC
     2581 AGGAGTTCAT CCGTCAAGTT CAAGCCAGTT ACCCTCATCT ACTTGAACAG CTGCTATCCA
     2641 CATCAGACAG CCCAGGAGAT GAAAATGCAG AGTCATCAAT TATCCATTTT GAACCTGGAG
     2701 AAGACCATTC AGAAGATGCA ATCATGATGA ATACTCCTGT GATTAATGCT GCCGTGGAAA
     2761 TGGGCTTTAG TAGAAGCCTG GTAAAACAGA CAGTTCAGAG AAAAATCCTA GCAACTGGAG
     2821 AGAATTATAG ACTAGTCAAT GATCTTGTGT TAGACTTACT CAATGCAGAA GATGAAATAA
     2881 GGGAAGAGGA GAGAGAAAGA GCAACTGAGG AAAAAGAATC AAATGATTTA TTATTAATCC
     2941 GGAAGAATAG AATGGCACTT TTTCAACATT TGACTTGTGT AATTCCAATC CTGGATAGTC
     3001 TACTAACTGC CGGAATTATT AATGAACAAG AACATGATGT TATTAAACAG AAGACACAGA
     3061 CGTCTTTACA AGCAAGAGAA CTGATTGATA CGATTTTAGT AAAAGGAAAT ATTGCAGCCA
     3121 CTGTATTCAG AAACTCTCTG CAAGAAGCTG AAGCTGTGTT ATATGAGCAT TTATTTGTGC
     3181 AACAGGACAT AAAATATATT CCCACAGAAG ATGTTTCAGA TCTACCAGTG GAAGAACAAT
     3241 TGCGGAGACT ACAAGAAGAA AGAACATGTA AAGTGTGTAT GGACAAAGAA GTGTCCATAG
     3301 TGTTTATTCC TTGTGGTCAT CTAGTAGTAT GCAAAGATTG TGCTCCTTCT TTAAGAAAGT
     3361 GTCCTATTTG TAGGAGTACA ATCAAGGGTA CAGTTCGTAC ATTTCTTTCA GTTTAAACGA
     3421 ATTCGGGCTC GGTACCCGGG GATCCTCTAG AGTCGACCTG CAGGCATGCA AGCTGATCCG
     3481 GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA
     3541 GCATAACCCC TTGGGGCGGC CGCTTCGAGC AGACATGATA AGATACATTG ATGAGTTTGG
     3601 ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT
     3661 TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA
     3721 TTTTATGTTT CAGGTTCAGG GGGAGATGTG GGAGGTTTTT TTAAGCAAGT AAAACCTCTA
     3781 CAAATGTGGT AAAATCGAAT TTTAACAAAA TATTAACGCT TACAATTTCC TGATGCGGTA
     3841 TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA CGCGGATCTG CGCAGCACCA
     3901 TGGCCTGAAA TAACCTCTGA AAGAGGAACT TGGTTAGGTA CCTTCTGAGG CGGAAAGAAC
     3961 CAGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA
     4021 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC
     4081 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC
     4141 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC
     4201 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG
     4261 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTTAA TTAACTGTTG
     4321 ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA
     4381 CCCAGGAGGC AGATCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG
     4441 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC
     4501 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT
     4561 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT
     4621 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC
     4681 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC
     4741 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC
     4801 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG
     4861 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG
     4921 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT
     4981 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG
     5041 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA
     5101 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC
     5161 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC
     5221 AAGCGACGCC CAACCTGCCA TCACGATGGC CGCAATAAAA TATCTTTATT TTCATTACAT
     5281 CTGTGTGTTG GTTTTTTGTG TGAATCGATA GCGATAAGGA TCCTCTTTGC GCTTGCGTTT
     5341 TCCCTTGTCC AGATAGCCCA GTAGCTGACA TTCATCCGGG GTCAGCACCG TTTCTGCGGA
     5401 CTGGCTTTCT ACCCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA
     5461 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA
     5521 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
     5581 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
     5641 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC
     5701 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT
     5761 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC
     5821 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG
     5881 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA
     5941 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC
     6001 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA
     6061 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA
     6121 GGATTTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     6181 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     6241 TAGTCCGGAA ATACAGGAAC GCACGCTGGA TGGCCCTTCG CTGGGATGGT GAAACCATGA
     6301 AAAATGGCAG CTTCAGTGGA TTAAGTGGGG GTAATGTGGC CTGTACCCTC TGGTTGCATA
     6361 GGTATTCATA CGGTTAAAAT TTATCAGGCG CGATTGCGGC AGTTTTTCGG GTGGTTTGTT
     6421 GCCATTTTTA CCTGTCTGCT GCCGTGATCG CGCTGAACGC GTTTTAGCGG TGCGTACAAT
     6481 TAAGGGATTA TGGTAAATCC ACTTACTGTC TGCCCTCGTA GCCATCGAGA TAAACCGCAG
     6541 TACTCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC T
//