LOCUS       Exported                5807 bp ds-DNA     circular SYN 26-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 26, 2021 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
COMMENT     LOCUS       pF-32Kp-MAP2K2      5807 bp    DNA   circular           
            5/31/2016

            DEFINITION  Current Name: pF-32Kp - MAP2K2
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1060..2268
                     /label=MAP2K2 insert
     misc_feature    2265..2271
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    2271..2285
                     /label=C Terminal NanoLuc linker
     misc_feature    2274..2285
                     /label=N Terminal NanoLuc linker
     CDS             2286..2795
                     /label=C terminal NanoLuc
     polyA_signal    2932..3153
                     /label=Sv40 late polyadenylation signal
     promoter        3252..3670
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(3325..3561)
                     /label=SV40 Enhancer
                     /note="Sequence shown is from SV40 genome. Our vectors 
                     contain sequences with one or two point mutations in first 
                     few bases. These apparently do not affect function. The 
                     sequences present in our vectors are shown to the right."
     rep_origin      3568..3633
                     /label=Sv40 Min Ori
     promoter        3678..3744
                     /label=EM7 bacterial promoter
     CDS             3758..4552
                     /label=Neomycin phosphotransferase coding
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4616..4664
                     /label=Synthetic PolyAdenylation Signal
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgctg gcccggagga
     1081 agccggtgct gccggcgctc accatcaacc ctaccatcgc cgagggccca tcccctacca
     1141 gcgagggcgc ctccgaggca aacctggtgg acctgcagaa gaagctggag gagctggaac
     1201 ttgacgagca gcagaagaag cggctggaag cctttctcac ccagaaagcc aaggtcggcg
     1261 aactcaaaga cgatgacttc gaaaggatct cagagctggg cgcgggcaac ggcggggtgg
     1321 tcaccaaagt ccagcacaga ccctcgggcc tcatcatggc caggaagctg atccaccttg
     1381 agatcaagcc ggccatccgg aaccagatca tccgcgagct gcaggtcctg cacgaatgca
     1441 actcgccgta catcgtgggc ttctacgggg ccttctacag tgacggggag atcagcattt
     1501 gcatggaaca catggacggc ggctccctgg accaggtgct gaaagaggcc aagaggattc
     1561 ccgaggagat cctggggaaa gtcagcatcg cggttctccg gggcttggcg tacctccgag
     1621 agaagcacca gatcatgcac cgagatgtga agccctccaa catcctcgtg aactctagag
     1681 gggagatcaa gctgtgtgac ttcggggtga gcggccagct catagactcc atggccaact
     1741 ccttcgtggg cacgcgctcc tacatggctc cggagcggtt gcagggcaca cattactcgg
     1801 tgcagtcgga catctggagc atgggcctgt ccctggtgga gctggccgtc ggaaggtacc
     1861 ccatcccccc gcccgacgcc aaagagctgg aggccatctt tggccggccc gtggtcgacg
     1921 gggaagaagg agagcctcac agcatctcgc ctcggccgag gccccccggg cgccccgtca
     1981 gcggtcacgg gatggatagc cggcctgcca tggccatctt tgaactcctg gactatattg
     2041 tgaacgagcc acctcctaag ctgcccaacg gtgtgttcac ccccgacttc caggagtttg
     2101 tcaataaatg cctcatcaag aacccagcgg agcgggcgga cctgaagatg ctcacaaacc
     2161 acaccttcat caagcggtcc gaggtggaag aagtggattt tgccggctgg ttgtgtaaaa
     2221 ccctgcggct gaaccagccc ggcacaccca cgcgcaccgc cgtggtttct ctcggctcga
     2281 gcggcgtctt cacactcgaa gatttcgttg gggactggcg acagacagcc ggctacaacc
     2341 tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt tcagaatctc ggggtgtccg
     2401 taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg gctgaagatc gacatccatg
     2461 tcatcatccc gtatgaaggt ctgagcggcg accaaatggg ccagatcgaa aaaattttta
     2521 aggtggtgta ccctgtggat gatcatcact ttaaggtgat cctgcactat ggcacactgg
     2581 taatcgacgg ggttacgccg aacatgatcg actatttcgg acggccgtat gaaggcatcg
     2641 ccgtgttcga cggcaaaaag atcactgtaa cagggaccct gtggaacggc aacaaaatta
     2701 tcgacgagcg cctgatcaac cccgacggct ccctgctgtt ccgagtaacc atcaacggag
     2761 tgaccggctg gcggctgtgc gaacgcattc tggcgtaagg ccgcgactct agagtcgacc
     2821 tgcaggcatg caagctgatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     2881 tgccaccgct gagcaataac tagcataacc ccttggggcg gccgcttcga gcagacatga
     2941 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     3001 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     3061 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
     3121 ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatcga attttaacaa aatattaacg
     3181 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3241 tacgcggatc tgcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg
     3301 taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3361 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3421 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3481 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3541 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3601 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3661 caaaaagctt aattaactgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     3721 tacgacaagg tgaggaacta aacccaggag gcagatcatg attgaacaag atggattgca
     3781 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     3841 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     3901 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     3961 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     4021 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     4081 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     4141 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     4201 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     4261 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     4321 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     4381 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     4441 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     4501 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     4561 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgatg gccgcaataa
     4621 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagcgataag
     4681 gatccgctca tgcgatgata agctgtcaaa catgagaatt acaacttata tcgtatgggg
     4741 ctgacttcag gtgctacatt tgaagagata aattgcactg aaatctagaa atattttatc
     4801 tgattaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa
     4861 aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg
     4921 aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct
     4981 taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag
     5041 tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5101 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc
     5161 ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg
     5221 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt
     5281 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct
     5341 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt
     5401 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg
     5461 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta
     5521 tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca
     5581 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgatgtc
     5641 cggcggtgct tttgccgtta cgcaccaccc cgtcatgaga ttacgcgttt tagcggtgcg
     5701 tacaattaag ggattatggt aaatccactt actgtctgcc ctcgtagcca tcgagataaa
     5761 ccgcagtact ccggccacga tgcgtccggc gtagaggatc gagatct
//
