LOCUS       Exported                9864 bp ds-DNA     circular SYN 25-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 16, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        4..383
                     /label=CMV enhancer
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1677..1695
                     /label=Raichu-F
     primer_bind     1688..1710
                     /label=pCAGGSseq_dir (№49)
     primer_bind     complement(1727..1747)
                     /label=common_ank_r
     misc_feature    1739..1744
                     /label=kozak
     CDS             1745..4261
                     /codon_start=1
                     /label=hTRPV1
                     /translation="MKKWSSTDLGAAADPLQKDTCPDPLDGDPNSRPPPAKPQLSTAKS
                     RTRLFGKGDSEEAFPVDCPHEEGELDSCPTITVSPVITIQRPGDGPTGARLLSQDSVAA
                     STEKTLRLYDRRSIFEAVAQNNCQDLESLLLFLQKSKKHLTDNEFKDPETGKTCLLKAM
                     LNLHDGQNTTIPLLLEIARQTDSLKELVNASYTDSYYKGQTALHIAIERRNMALVTLLV
                     ENGADVQAAAHGDFFKKTKGRPGFYFGELPLSLAACTNQLGIVKFLLQNSWQTADISAR
                     DSVGNTVLHALVEVADNTADNTKFVTSMYNEILILGAKLHPTLKLEELTNKKGMTPLAL
                     AAGTGKIGVLAYILQREIQEPECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEV
                     IAYSSSETPNRHDMLLVEPLNRLLQDKWDRFVKRIFYFNFLVYCLYMIIFTMAAYYRPV
                     DGLPPFKMEKIGDYFRVTGEILSVLGGVYFFFRGIQYFLQRRPSMKTLFVDSYSEMLFF
                     LQSLFMLATVVLYFSHLKEYVASMVFSLALGWTNMLYYTRGFQQMGIYAVMIEKMILRD
                     LCRFMFVYIVFLFGFSTAVVTLIEDGKNDSLPSESTSHRWRGPACRPPDSSYNSLYSTC
                     LELFKFTIGMGDLEFTENYDFKAVFIILLLAYVILTYILLLNMLIALMGETVNKIAQES
                     KNIWKLQRAITILDTEKSFLKCMRKAFRSGKLLQVGYTPDGKDDYRWCFRVDEVNWTTW
                     NTNVGIINEDPGNCEGVKRTLSFSLRSSRVSGRHWKNFALVPLLREASARDRQSAQPEE
                     VYLRQFSGSLKPEDAEVFKSPAASGEK"
     misc_feature    1745..2028
                     /label=1
     misc_feature    2029..2195
                     /label=2
     primer_bind     2075..2099
                     /label=ank1_f
     primer_bind     complement(2078..2106)
                     /label=hTRPAaddBpuR
     primer_bind     2102..2139
                     /label=Hum-N125N_F
     primer_bind     2102..2139
                     /label=Hum-N125S_F
     variation       2117..2119
                     /label=RedStar
     misc_feature    2196..2348
                     /label=3
     primer_bind     2204..2228
                     /label=ank2_f
     primer_bind     complement(2289..2322)
                     /label=Hum-E189Q_R
     primer_bind     complement(2289..2322)
                     /label=Hum-E189D_R
     primer_bind     complement(2296..2322)
                     /label=Hum-E189E_R
     variation       2309..2311
                     /label=YellowStar
     primer_bind     2313..2335
                     /label=hTRPAaddHpaF
     primer_bind     2345..2369
                     /label=ank3_f
     misc_feature    2349..2489
                     /label=4
     primer_bind     complement(2481..2502)
                     /label=hTRPV1_seq3r
     primer_bind     2486..2510
                     /label=ank4_f
     misc_feature    2490..2788
                     /label=5
     primer_bind     complement(2576..2593)
                     /label=-ank5_R
     primer_bind     complement(2720..2740)
                     /label=-ank6_R
     primer_bind     2741..2758
                     /label=-ank5_F
     misc_feature    2789..2968
                     /label=6
     misc_feature    2789..2968
                     /label=TRPV1b GAP
     primer_bind     2822..2838
                     /label=-ank6_F
     primer_bind     2944..2961
                     /label=hTRPV1_seq3f
     misc_feature    2969..3127
                     /label=7
     primer_bind     complement(3094..3114)
                     /label=hTRPV1_seq2r
     misc_feature    3128..3220
                     /label=8
     misc_feature    3221..3291
                     /label=9
     misc_feature    3292..3457
                     /label=10
     misc_feature    3458..3524
                     /label=11
     misc_feature    3525..3847
                     /label=12
     primer_bind     3563..3582
                     /label=hTRPV1_seq4f
     primer_bind     complement(3644..3664)
                     /label=TRPV1-pot_R2
     primer_bind     complement(3653..3674)
                     /label=TRPV1-pot_R3
     primer_bind     complement(3653..3670)
                     /label=TRPV1-pot_R1
     primer_bind     complement(3657..3676)
                     /label=TRPVpore_R
     primer_bind     3678..3694
                     /label=TRPV-M645L_F
     primer_bind     3680..3698
                     /label=TRPV1-pot_F1
     primer_bind     complement(3692..3718)
                     /label=hTRPV1_seq1r
     misc_feature    3848..3975
                     /label=13
     misc_feature    3976..4091
                     /label=14
     misc_feature    4092..4261
                     /label=15
     CDS             4265..4330
                     /codon_start=1
                     /label=P2A
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     primer_bind     4273..4297
                     /label=tdTom_F-Bsp1407I
     CDS             4331..5761
                     /codon_start=1
                     /label=tdTomato
                     /label=td tomato
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(4350..4373)
                     /label=tdTom-Rv2
     primer_bind     complement(5078..5099)
                     /label=tdTom-Rv2
     intron          5787..5920
                     /label=chimeric intron
     misc_feature    5929..6517
                     /label=WPRE
     CDS             complement(6400..6411)
                     /codon_start=1
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6550..6661
                     /label=bGH poly(A) signal
     polyA_site      6953..7008
                     /label=beta-globin poly(A) signal
     primer_bind     complement(7367..7383)
                     /label=M13 rev
     protein_bind    7391..7407
                     /label=lac operator
     promoter        complement(7415..7421)
                     /label=lac promoter
     promoter        complement(7422..7439)
                     /label=lac promoter
     promoter        complement(7440..7445)
                     /label=lac promoter
     protein_bind    7460..7481
                     /label=CAP binding site
     promoter        7540..7735
                     /label=SV40 promoter
     rep_origin      7586..7721
                     /label=SV40 ori
     polyA_signal    7741..7875
                     /label=SV40 poly(A) signal
     rep_origin      complement(8114..8702)
                     /direction=LEFT
                     /label=ori
     CDS             complement(8873..9664)
                     /codon_start=1
                     /label=AmpR
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMS
                     TFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITM
                     SDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMAT
                     TLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIA
                     ALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             complement(9665..9733)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFA"
     promoter        complement(9734..9838)
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg ggcgagggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcggtacc gcgggcccgc
     1741 caccatgaag aaatggagca gcacagactt gggggcagct gcggacccac tccaaaagga
     1801 cacctgccca gaccccctgg atggagaccc taactccagg ccacctccag ccaagcccca
     1861 gctctccacg gccaagagcc gcacccggct ctttgggaag ggtgactcgg aggaggcttt
     1921 cccggtggat tgccctcacg aggaaggtga gctggactcc tgcccgacca tcacagtcag
     1981 ccctgttatc accatccaga ggccaggaga cggccccacc ggtgccaggc tgctgtccca
     2041 ggactctgtc gccgccagca ccgagaagac cctcaggctc tatgatcgca ggagtatctt
     2101 tgaagccgtt gctcagaata actgccagga tctggagagc ctgctgctct tcctgcagaa
     2161 gagcaagaag cacctcacag acaacgagtt caaagaccct gagacaggga agacctgtct
     2221 gctgaaagcc atgctcaacc tgcatgacgg acagaacacc accatccccc tgctcctgga
     2281 gatcgcgcgg caaacggaca gcctgaagga gcttgtcaac gccagctaca cggacagcta
     2341 ctacaagggc cagacagcac tgcacatcgc catcgagaga cgcaacatgg ccctggtgac
     2401 cctcctggtg gagaacggag cagacgtcca ggctgcggcc catggggact tctttaagaa
     2461 aaccaaaggg cggcctggat tctacttcgg tgaactgccc ctgtccctgg ccgcgtgcac
     2521 caaccagctg ggcatcgtga agttcctgct gcagaactcc tggcagacgg ccgacatcag
     2581 cgccagggac tcggtgggca acacggtgct gcacgccctg gtggaggtgg ccgacaacac
     2641 ggccgacaac acgaagtttg tgacgagcat gtacaatgag attctgatcc tgggggccaa
     2701 actgcacccg acgctgaagc tggaggagct caccaacaag aagggaatga cgccgctggc
     2761 tctggcagct gggaccggga agatcggggt cttggcctat attctccagc gggagatcca
     2821 ggagcccgag tgcaggcacc tgtccaggaa gttcaccgag tgggcctacg ggcccgtgca
     2881 ctcctcgctg tacgacctgt cctgcatcga cacctgcgag aagaactcgg tgctggaggt
     2941 gatcgcctac agcagcagcg agacccctaa tcgccacgac atgctcttgg tggagccgct
     3001 gaaccgactc ctgcaggaca agtgggacag attcgtcaag cgcatcttct acttcaactt
     3061 cctggtctac tgcctgtaca tgatcatctt caccatggct gcctactaca ggcccgtgga
     3121 tggcttgcct ccctttaaga tggaaaaaat tggagactat ttccgagtta ctggagagat
     3181 cctgtctgtg ttaggaggag tctacttctt tttccgaggg attcagtatt tcctgcagag
     3241 gcggccgtcg atgaagaccc tgtttgtgga cagctacagt gagatgcttt tctttctgca
     3301 gtcactgttc atgctggcca ccgtggtgct gtacttcagc cacctcaagg agtatgtggc
     3361 ttccatggta ttctccctgg ccttgggctg gaccaacatg ctctactaca cccgcggttt
     3421 ccagcagatg ggcatctatg ccgtcatgat agagaagatg atcctgagag acctgtgccg
     3481 tttcatgttt gtctacatcg tcttcttgtt cgggttttcc acagcggtgg tgacgctgat
     3541 tgaagacggg aagaatgact ccctgccgtc tgagtccacg tcgcacaggt ggcgggggcc
     3601 tgcctgcagg ccccccgata gctcctacaa cagcctgtac tccacctgcc tggagctgtt
     3661 caagttcacc atcggcatgg gcgacctgga gttcactgag aactatgact tcaaggctgt
     3721 cttcatcatc ctgctgctgg cctatgtaat tctcacctac atcctcctgc tcaacatgct
     3781 catcgccctc atgggtgaga ctgtcaacaa gatcgcacag gagagcaaga acatctggaa
     3841 gctgcagaga gccatcacca tcctggacac ggagaagagc ttccttaagt gcatgaggaa
     3901 ggccttccgc tcaggcaagc tgctgcaggt ggggtacaca cctgatggca aggacgacta
     3961 ccggtggtgc ttcagggtgg acgaggtgaa ctggaccacc tggaacacca acgtgggcat
     4021 catcaacgaa gacccgggca actgtgaggg cgtcaagcgc accctgagct tctccctgcg
     4081 gtcaagcaga gtttcaggca gacactggaa gaactttgcc ctggtccccc ttttaagaga
     4141 ggcaagtgct cgagataggc agtctgctca gcccgaggaa gtttatctgc gacagttttc
     4201 agggtctctg aagccagagg acgctgaggt cttcaagagt cctgccgctt ccggggagaa
     4261 ggcgggaagc ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa
     4321 ccctggacct atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt
     4381 gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
     4441 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
     4501 cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc
     4561 cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     4621 gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg
     4681 cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat
     4741 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     4801 gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga
     4861 gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga
     4921 caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
     4981 ctccgagggc cgccaccacc tgttcctggg gcatggcacc ggcagcaccg gcagcggcag
     5041 ctccggcacc gcctcctccg aggacaacaa catggccgtc atcaaagagt tcatgcgctt
     5101 caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
     5161 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
     5221 gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa
     5281 gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga
     5341 gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca
     5401 ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc
     5461 cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga
     5521 cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct
     5581 ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta
     5641 cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     5701 cgagcgctcc gagggccgcc accacctgtt cctgtacggc atggacgagc tgtacaagta
     5761 aagcggcccc ggactcgagg ccgcaggtaa gtatcaaggt tacaagacag gtttaaggag
     5821 accacataga aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct
     5881 attggtctta ctgacatcca ctttgccttt ctctccacag gtgtcgacaa tcaacctctg
     5941 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6001 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6061 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6121 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6181 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6241 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6301 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     6361 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6421 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6481 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacgat
     6541 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6601 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6661 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     6721 gggaggattg ggaagacaat agcaggccag cttttgttcc ctttagggtt aattgcgcgc
     6781 ttggcgctaa tcatggtcat agctgtttcc actcctcagg tgcaggctgc ctatcagaag
     6841 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
     6901 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     6961 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     7021 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     7081 tatgccatat gctggctgcc atgaacaaag gtggctataa agaggtcatc agtatatgaa
     7141 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     7201 tttttatatt ttgtttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     7261 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     7321 cttatgaaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc
     7381 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7441 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7501 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac
     7561 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7621 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7681 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7741 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7801 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7861 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7921 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7981 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8041 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8221 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8281 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     8341 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8401 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8461 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8521 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8581 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8641 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8701 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8761 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8821 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8881 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8941 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9001 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9061 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9121 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9181 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9241 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9301 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9361 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9421 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9481 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9541 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9601 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9661 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9721 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9781 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9841 catttccccg aaaagtgcca cctg
//
