LOCUS       Exported                7292 bp ds-DNA     circular SYN 19-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 16, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     150..181
                     /label=Юра*F_CamkII_Mlu
     misc_feature    156..1448
                     /label=a-CaMKII(1.3)
                     /note="/vntifkey=21"
     primer_bind     442..459
                     /label=Юра*F_CamK
     primer_bind     1411..1427
                     /label=Лена*pLenti-CamK-F
     primer_bind     complement(1431..1454)
                     /label=Юра*R_CamkII_Nhe
     primer_bind     1449..1484
                     /label=TRPV1_Kpn(Nhe)_F
     misc_feature    1455..1460
                     /label=Kozak
     CDS             1461..3977
                     /codon_start=1
                     /label=hTRPV1
                     /translation="MKKWSSTDLGAAADPLQKDTCPDPLDGDPNSRPPPAKPQLSTAKS
                     RTRLFGKGDSEEAFPVDCPHEEGELDSCPTITVSPVITIQRPGDGPTGARLLSQDSVAA
                     STEKTLRLYDRRSIFEAVAQNNCQDLESLLLFLQKSKKHLTDNEFKDPETGKTCLLKAM
                     LNLHDGQNTTIPLLLEIARQTDSLKELVNASYTDSYYKGQTALHIAIERRNMALVTLLV
                     ENGADVQAAAHGDFFKKTKGRPGFYFGELPLSLAACTNQLGIVKFLLQNSWQTADISAR
                     DSVGNTVLHALVEVADNTADNTKFVTSMYNEILILGAKLHPTLKLEELTNKKGMTPLAL
                     AAGTGKIGVLAYILQREIQEPECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEV
                     IAYSSSETPNRHDMLLVEPLNRLLQDKWDRFVKRIFYFNFLVYCLYMIIFTMAAYYRPV
                     DGLPPFKMEKIGDYFRVTGEILSVLGGVYFFFRGIQYFLQRRPSMKTLFVDSYSEMLFF
                     LQSLFMLATVVLYFSHLKEYVASMVFSLALGWTNMLYYTRGFQQMGIYAVMIEKMILRD
                     LCRFMFVYIVFLFGFSTAVVTLIEDGKNDSLPSESTSHRWRGPACRPPDSSYNSLYSTC
                     LELFKFTIGMGDLEFTENYDFKAVFIILLLAYVILTYILLLNMLIALMGETVNKIAQES
                     KNIWKLQRAITILDTEKSFLKCMRKAFRSGKLLQVGYTPDGKDDYRWCFRVDEVNWTTW
                     NTNVGIINEDPGNCEGVKRTLSFSLRSSRVSGRHWKNFALVPLLREASARDRQSAQPEE
                     VYLRQFSGSLKPEDAEVFKSPAASGEK"
     primer_bind     1791..1815
                     /label=ank1_f
     primer_bind     complement(1794..1822)
                     /label=hTRPAaddBpuR
     variation       1833..1835
                     /label=RedStar
     primer_bind     1920..1944
                     /label=ank2_f
     variation       2025..2027
                     /label=YellowStar
     primer_bind     2029..2051
                     /label=hTRPAaddHpaF
     primer_bind     2061..2085
                     /label=ank3_f
     primer_bind     complement(2197..2218)
                     /label=hTRPV1_seq3r
     primer_bind     2202..2226
                     /label=ank4_f
     primer_bind     complement(2292..2309)
                     /label=-ank5_R
     primer_bind     complement(2436..2456)
                     /label=-ank6_R
     primer_bind     2457..2474
                     /label=-ank5_F
     primer_bind     2538..2554
                     /label=-ank6_F
     primer_bind     2660..2677
                     /label=hTRPV1_seq3f
     primer_bind     complement(2810..2830)
                     /label=hTRPV1_seq2r
     primer_bind     complement(2810..2830)
                     /label=hTRPV1_seq2ra
     primer_bind     3279..3298
                     /label=hTRPV1_seq4f(Tm=61.2)
     primer_bind     3279..3298
                     /label=hTRPV1_seq4f
     primer_bind     complement(3408..3434)
                     /label=hTRPV1_seq1r
     CDS             3978..4001
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    4039..4515
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4530..4547)
                     /label=Юра*R1_aft_PolyA
     repeat_region   4555..4695
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4770..5225
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5507..5611
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5612..6472
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6643..7231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7226..7244
                     /label=Юра_F2_after_ori
     primer_bind     7258..7276
                     /label=Юра*F_after_ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt
      181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt
      241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt
      301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact
      361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca
      421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc
      481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat
      541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc
      601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat
      661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct
      721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc
      781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga
      841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac
      901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc
      961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa
     1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat
     1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
     1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
     1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
     1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
     1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
     1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
     1441 ggggcagcgc tagcgccacc atgaagaaat ggagcagcac agacttgggg gcagctgcgg
     1501 acccactcca aaaggacacc tgcccagacc ccctggatgg agaccctaac tccaggccac
     1561 ctccagccaa gccccagctc tccacggcca agagccgcac ccggctcttt gggaagggtg
     1621 actcggagga ggctttcccg gtggattgcc ctcacgagga aggtgagctg gactcctgcc
     1681 cgaccatcac agtcagccct gttatcacca tccagaggcc aggagacggc cccaccggtg
     1741 ccaggctgct gtcccaggac tctgtcgccg ccagcaccga gaagaccctc aggctctatg
     1801 atcgcaggag tatctttgaa gccgttgctc agaataactg ccaggatctg gagagcctgc
     1861 tgctcttcct gcagaagagc aagaagcacc tcacagacaa cgagttcaaa gaccctgaga
     1921 cagggaagac ctgtctgctg aaagccatgc tcaacctgca tgacggacag aacaccacca
     1981 tccccctgct cctggagatc gcgcggcaaa cggacagcct gaaggagctt gtcaacgcca
     2041 gctacacgga cagctactac aagggccaga cagcactgca catcgccatc gagagacgca
     2101 acatggccct ggtgaccctc ctggtggaga acggagcaga cgtccaggct gcggcccatg
     2161 gggacttctt taagaaaacc aaagggcggc ctggattcta cttcggtgaa ctgcccctgt
     2221 ccctggccgc gtgcaccaac cagctgggca tcgtgaagtt cctgctgcag aactcctggc
     2281 agacggccga catcagcgcc agggactcgg tgggcaacac ggtgctgcac gccctggtgg
     2341 aggtggccga caacacggcc gacaacacga agtttgtgac gagcatgtac aatgagattc
     2401 tgatcctggg ggccaaactg cacccgacgc tgaagctgga ggagctcacc aacaagaagg
     2461 gaatgacgcc gctggctctg gcagctggga ccgggaagat cggggtcttg gcctatattc
     2521 tccagcggga gatccaggag cccgagtgca ggcacctgtc caggaagttc accgagtggg
     2581 cctacgggcc cgtgcactcc tcgctgtacg acctgtcctg catcgacacc tgcgagaaga
     2641 actcggtgct ggaggtgatc gcctacagca gcagcgagac ccctaatcgc cacgacatgc
     2701 tcttggtgga gccgctgaac cgactcctgc aggacaagtg ggacagattc gtcaagcgca
     2761 tcttctactt caacttcctg gtctactgcc tgtacatgat catcttcacc atggctgcct
     2821 actacaggcc cgtggatggc ttgcctccct ttaagatgga aaaaattgga gactatttcc
     2881 gagttactgg agagatcctg tctgtgttag gaggagtcta cttctttttc cgagggattc
     2941 agtatttcct gcagaggcgg ccgtcgatga agaccctgtt tgtggacagc tacagtgaga
     3001 tgcttttctt tctgcagtca ctgttcatgc tggccaccgt ggtgctgtac ttcagccacc
     3061 tcaaggagta tgtggcttcc atggtattct ccctggcctt gggctggacc aacatgctct
     3121 actacacccg cggtttccag cagatgggca tctatgccgt catgatagag aagatgatcc
     3181 tgagagacct gtgccgtttc atgtttgtct acatcgtctt cttgttcggg ttttccacag
     3241 cggtggtgac gctgattgaa gacgggaaga atgactccct gccgtctgag tccacgtcgc
     3301 acaggtggcg ggggcctgcc tgcaggcccc ccgatagctc ctacaacagc ctgtactcca
     3361 cctgcctgga gctgttcaag ttcaccatcg gcatgggcga cctggagttc actgagaact
     3421 atgacttcaa ggctgtcttc atcatcctgc tgctggccta tgtaattctc acctacatcc
     3481 tcctgctcaa catgctcatc gccctcatgg gtgagactgt caacaagatc gcacaggaga
     3541 gcaagaacat ctggaagctg cagagagcca tcaccatcct ggacacggag aagagcttcc
     3601 ttaagtgcat gaggaaggcc ttccgctcag gcaagctgct gcaggtgggg tacacacctg
     3661 atggcaagga cgactaccgg tggtgcttca gggtggacga ggtgaactgg accacctgga
     3721 acaccaacgt gggcatcatc aacgaagacc cgggcaactg tgagggcgtc aagcgcaccc
     3781 tgagcttctc cctgcggtca agcagagttt caggcagaca ctggaagaac tttgccctgg
     3841 tccccctttt aagagaggca agtgctcgag ataggcagtc tgctcagccc gaggaagttt
     3901 atctgcgaca gttttcaggg tctctgaagc cagaggacgc tgaggtcttc aagagtcctg
     3961 ccgcttccgg ggagaaggat tacaaggacg acgacgataa gtaagaattc ctcgaggagc
     4021 gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
     4081 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
     4141 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
     4201 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
     4261 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
     4321 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
     4381 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
     4441 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
     4501 cccttccctg tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac
     4561 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     4621 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     4681 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     4741 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     4801 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4861 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4921 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4981 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5041 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5101 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5161 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     5221 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     5281 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     5341 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     5401 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5461 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5521 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5581 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5641 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5701 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5761 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5821 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5881 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5941 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     6001 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     6061 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6121 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6181 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6241 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6301 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6361 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6421 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6481 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6541 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6601 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6661 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6721 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6781 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6841 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6901 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6961 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7021 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7081 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7141 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7201 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7261 cctggccttt tgctggcctt ttgctcacat gt
//
