LOCUS       AAV9_GFAP_HyPer7_mito   7005 bp ds-DNA     circular     15-OCT-2024
DEFINITION  .
KEYWORDS    "marker:AAV2 ITR, AAV2 ITR, AmpR"
FEATURES             Location/Qualifiers
     repeat_region   1..141
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     misc_feature    156..2074
                     /label="GFAP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             2087..2161
                     /label="COX8 presequence"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="EC_number: codon_start: 1 gene: product: mitochondrial presequence of human cytochrome c oxidase subunit VIII (Rizutto et al., 1989) protein_id: transl_table: 1 translation: MSVLTPLLLRGLTGSARRLPVPRAK"
     misc_feature    2087..3715
                     /label="mitoHyPer7"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             2183..2254
                     /label="COX8 presequence"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="EC_number: codon_start: 1 gene: product: mitochondrial presequence of human cytochrome c oxidase subunit VIII (Rizutto et al., 1989) protein_id: transl_table: 1 translation: SVLTPLLLRGLTGSARRLPVPRAK"
     polyA_signal    3752..4228
                     /label="hGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: human growth hormone polyadenylation signal"
     repeat_region   4268..4408
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     rep_origin      4483..4938
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        5220..5324
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             5325..5393
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             5394..6185
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      6356..6944
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtCTTAA GGCTGGGAGG TTGAGGCTGC
      181 AGTGAGTCGT GGTTGCGCCA CTGCACTCCA GCCTGGGCAA CAGTGAGACC CTGTCTCAAA
      241 AGACAAAAAA AAAAAAAAAA AAAAAAAGAA CATATCCTGG TGTGGAGTAG GGGACGCTGC
      301 TCTGACAGAG GCTCGGGGGC CTGAGCTGGC TCTGTGAGCT GGGGAGGAGG CAGACAGCCA
      361 GGCCTTGTCT GCAAGCAGAC CTGGCAGCAT TGGGCTGGCC GCCCCCCAGG GCCTCCTCTT
      421 CATGCCCAGT GAATGACTCA CCTTGGCACA GACACAATGT TCGGGGTGGG CACAGTGCCT
      481 GCTTCCCGCC GCACCCCAGC CCCCCTCAAA TGCCTTCCGA GAAGCCCATT GAGCAGGGGG
      541 CTTGCATTGC ACCCCAGCCT GACAGCCTGG CATCTTGGGA TAAAAGCAGC ACAGCCCCCT
      601 AGGGGCTGCC CTTGCTGTGT GGCGCCACCG GCGGTGGAGA ACAAGGCTCT ATTCAGCCTG
      661 TGCCCAGGAA AGGGGATCAG GGGATGCCCA GGCATGGACA GTGGGTGGCA GGGGGGGAGA
      721 GGAGGGCTGT CTGCTTCCCA GAAGTCCAAG GACACAAATG GGTGAGGGGA CTGGGCAGGG
      781 TTCTGACCCT GTGGGACCAG AGTGGAGGGC GTAGATGGAC CTGAAGTCTC CAGGGACAAC
      841 AGGGCCCAGG TCTCAGGCTC CTAGTTGGGC CCAGTGGCTC CAGCGTTTCC AAACCCATCC
      901 ATCCCCAGAG GTTCTTCCCA TCTCTCCAGG CTGATGTGTG GGAACTCGAG GAAATAAATC
      961 TCCAGTGGGA GACGGAGGGG TGGCCAGGGA AACGGGGCGC TGCAGGAATA AAGACGAGCC
     1021 AGCACAGCCA GCTCATGTGT AACGGCTTTG TGGAGCTGTC AAGGCCTGGT CTCTGGGAGA
     1081 GAGGCACAGG GAGGCCAGAC AAGGAAGGGG TGACCTGGAG GGACAGATCC AGGGGCTAAA
     1141 GTCCTGATAA GGCAAGAGAG TGCCGGCCCC CTCTTGCCCT ATCAGGACCT CCACTGCCAC
     1201 ATAGAGGCCA TGATTGACCC TTAGACAAAG GGCTGGTGTC CAATCCCAGC CCCCAGCCCC
     1261 AGAACTCCAG GGAATGAATG GGCAGAGAGC AGGAATGTGG GACATCTGTG TTCAAGGGAA
     1321 GGACTCCAGG AGTCTGCTGG GAATGAGGCC TAGTAGGAAA TGAGGTGGCC CTTGAGGGTA
     1381 CAGAACAGGT TCATTCTTCG CCAAATTCCC AGCACCTTGC AGGCACTTAC AGCTGAGTGA
     1441 GATAATGCCT GGGTTATGAA ATCAAAAAGT TGGAAAGCAG GTCAGAGGTC ATCTGGTACA
     1501 GCCCTTCCTT CCCTTTTTTT TTTTTTTTTT TTGTGAGACA AGGTCTCTCT CTGTTGCCCA
     1561 GGCTGGAGTG GCGCAAACAC AGCTCACTGC AGCCTCAACC TACTGGGCTC AAGCAATCCT
     1621 CCAGCCTCAG CCTCCCAAAG TGCTGGGATT ACAAGCATGA GCCACCCCAC TCAGCCCTTT
     1681 CCTTCCTTTT TAATTGATGC ATAATAATTG TAAGTATTCA TCATGGTCCA ACCAACCCTT
     1741 TCTTGACCCA CCTTCCTAGA GAGAGGGTCC TCTTGCTTCA GCGGTCAGGG CCCCAGACCC
     1801 ATGGTCTGGC TCCAGGTACC ACCTGCCTCA TGCAGGAGTT GGCGTGCCCA GGAAGCTCTG
     1861 CCTCTGGGCA CAGTGACCTC AGTGGGGTGA GGGGAGCTCT CCCCATAGCT GGGCTGCGGC
     1921 CCAaccccac cccctcaggc tatgccaggg ggtgttgcca ggggcacccg ggcatcgcca
     1981 gtctagccca ctccttcata aagccctcgc atcccaggag cgagcagagc cagagcaggt
     2041 tggagaggag acgcatcacc tccgctgctc gcGGgaattc gccaccATGt ccgtcctgac
     2101 gccgctgctg ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc cgcgcgccaa
     2161 gatccattcg ttgggggatc tgtccgtcct gacgccgctg ctgctgcggg gcttgacagg
     2221 ctcggcccgg cggctcccag tgccgcgcgc caagatccat tcgttggggg atACCGGTCA
     2281 CCTGGCTAAT GAGGAGCAAA ACGAATTGGA AGGTGCTTTT AAGCTGGGCT TGATCTTTAC
     2341 CGTCGCCCCG TACTTGCTGC CTAAGTTGAT CGTCAGCCTG CGCCGCACCG CCCCGAAGAT
     2401 GCCTTTGATG TTGGAGGAGA ATTACACCCA CACCCTTACC GAGTCACTGA AACGCGGCGA
     2461 CGTTGACGCC ATCATTGTGG CAGAGCCGTT TCAAGAGCCG GGTATTGTGA CTGAACCTTT
     2521 GTATGACGAG CCGTTTTTCG TTATCGTTCC GAAGGGCCAT AGCTTCGAGG AGTTGGATGC
     2581 CGTTAGCCCT CGCATGTTGG GCGAAGAACA AGTTCTGCTT CTGACCGAGG GCAACTGTAT
     2641 GCGTGACCAG GTGCTTAGCT ccgccggctt caacagcaac aacgtctata tcatggccga
     2701 caagcagaag aacggcatca aggccaactt caagatccgc cacaacgtcg aggacggcag
     2761 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2821 gcccgacaac cactacctga gcttccagtc cgtcctgagc aaagacccca acgagaagcg
     2881 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2941 gctgtacaac gtggatggcg gtagcggtgg caccggcagc aagggcgagg agctgttcac
     3001 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     3061 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac
     3121 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctcagct acggcctgaa
     3181 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     3241 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     3301 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcgg
     3361 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacggcc ccAGTTGCAG
     3421 CGAGCTTGCC GCCAAACAGC GCATTCAGGG TCTCACCAAC ACCCTTCAAG GCTCAAGCAT
     3481 CAATACCATC CGCCACATGG TTGCCAGTGG CTTGGCAATC AGCGTCTTGC CTGCCACTGC
     3541 CCTTACCGAA AACGACCACA TGCTGTTTAG CATTATCCCG TTTGAAGGCA CCCCGCCTAG
     3601 CCGCCGCGTT GTCCTGGCCT ATCGCCGCAA CTTTGTCCGC CCTAAGGCCC TTTCAGCCAT
     3661 GAAAGCCGCA ATTATGCAAA GCCAACTGCA TGGTGTTAGC TTCATCTGCG ATTGAaagct
     3721 tgcctcgagc agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
     3781 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     3841 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     3901 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     3961 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     4021 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     4081 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     4141 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     4201 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag
     4261 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     4321 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     4381 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     4441 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     4501 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4561 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4621 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4681 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4741 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4801 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     4861 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4921 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4981 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5041 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5101 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5161 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5221 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5281 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5341 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5401 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5461 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5521 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5581 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5641 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5701 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5761 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5821 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5881 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5941 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6001 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6061 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6121 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6181 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6241 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6301 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6361 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6421 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6481 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6541 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6601 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6661 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6721 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6781 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6841 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6901 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6961 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//