LOCUS       AAV9_hSyn_HyPer7_mito   5845 bp ds-DNA     circular     15-OCT-2024
DEFINITION  .
KEYWORDS    "marker:AAV2 ITR, AAV2 ITR, AmpR"
FEATURES             Location/Qualifiers
     repeat_region   1..141
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     enhancer        156..459
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus immediate early enhancer"
     promoter        467..914
                     /label="hSyn promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human synapsin I promoter; confers neuron-specific expression (Kügler et al., 2003)"
     CDS             927..1001
                     /label="COX8 presequence"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="EC_number: codon_start: 1 gene: product: mitochondrial presequence of human cytochrome c oxidase subunit VIII (Rizutto et al., 1989) protein_id: transl_table: 1 translation: MSVLTPLLLRGLTGSARRLPVPRAK"
     misc_feature    927..2555
                     /label="mitoHyPer7"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1023..1094
                     /label="COX8 presequence"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="EC_number: codon_start: 1 gene: product: mitochondrial presequence of human cytochrome c oxidase subunit VIII (Rizutto et al., 1989) protein_id: transl_table: 1 translation: SVLTPLLLRGLTGSARRLPVPRAK"
     polyA_signal    2592..3068
                     /label="hGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: human growth hormone polyadenylation signal"
     repeat_region   3108..3248
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     rep_origin      3323..3778
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        4060..4164
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             4165..4233
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             4234..5025
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      5196..5784
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtCGTTA CATAACTTAC GGTAAATGGC
      181 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC
      241 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT
      301 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT
      361 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT
      421 TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGg CTCGAGagtg caagtgggtt
      481 ttaggaccag gatgaggcgg ggtgggggtg cctacctgac gaccgacccc gacccactgg
      541 acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg gggaggggaa
      601 acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca gtgccttcgc
      661 ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg acgtcactcg
      721 ccggtccccc gcaaactccc cttcccggcc accttggtcg cgtccgcgcc gccgccggcc
      781 cagccggacc gcaccacgcg aggcgcgaga taggggggca cgggcgcgac catctgcgct
      841 gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt
      901 gcctgagagc gcaggaattc gccaccATGt ccgtcctgac gccgctgctg ctgcggggct
      961 tgacaggctc ggcccggcgg ctcccagtgc cgcgcgccaa gatccattcg ttgggggatc
     1021 tgtccgtcct gacgccgctg ctgctgcggg gcttgacagg ctcggcccgg cggctcccag
     1081 tgccgcgcgc caagatccat tcgttggggg atACCGGTCA CCTGGCTAAT GAGGAGCAAA
     1141 ACGAATTGGA AGGTGCTTTT AAGCTGGGCT TGATCTTTAC CGTCGCCCCG TACTTGCTGC
     1201 CTAAGTTGAT CGTCAGCCTG CGCCGCACCG CCCCGAAGAT GCCTTTGATG TTGGAGGAGA
     1261 ATTACACCCA CACCCTTACC GAGTCACTGA AACGCGGCGA CGTTGACGCC ATCATTGTGG
     1321 CAGAGCCGTT TCAAGAGCCG GGTATTGTGA CTGAACCTTT GTATGACGAG CCGTTTTTCG
     1381 TTATCGTTCC GAAGGGCCAT AGCTTCGAGG AGTTGGATGC CGTTAGCCCT CGCATGTTGG
     1441 GCGAAGAACA AGTTCTGCTT CTGACCGAGG GCAACTGTAT GCGTGACCAG GTGCTTAGCT
     1501 ccgccggctt caacagcaac aacgtctata tcatggccga caagcagaag aacggcatca
     1561 aggccaactt caagatccgc cacaacgtcg aggacggcag cgtgcagctc gccgaccact
     1621 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1681 gcttccagtc cgtcctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1741 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaac gtggatggcg
     1801 gtagcggtgg caccggcagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1861 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1921 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
     1981 cctggcccac cctcgtgacc accctcagct acggcctgaa gtgcttcgcc cgctaccccg
     2041 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     2101 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     2161 gcgacaccct ggtgaaccgc atcgagctga agggcatcgg cttcaaggag gacggcaaca
     2221 tcctggggca caagctggag tacaacggcc ccAGTTGCAG CGAGCTTGCC GCCAAACAGC
     2281 GCATTCAGGG TCTCACCAAC ACCCTTCAAG GCTCAAGCAT CAATACCATC CGCCACATGG
     2341 TTGCCAGTGG CTTGGCAATC AGCGTCTTGC CTGCCACTGC CCTTACCGAA AACGACCACA
     2401 TGCTGTTTAG CATTATCCCG TTTGAAGGCA CCCCGCCTAG CCGCCGCGTT GTCCTGGCCT
     2461 ATCGCCGCAA CTTTGTCCGC CCTAAGGCCC TTTCAGCCAT GAAAGCCGCA ATTATGCAAA
     2521 GCCAACTGCA TGGTGTTAGC TTCATCTGCG ATTGAaagct tgcctcgagc agcgctgctc
     2581 gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
     2641 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
     2701 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
     2761 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
     2821 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
     2881 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
     2941 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
     3001 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
     3061 ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt
     3121 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     3181 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     3241 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3301 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3361 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     3421 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     3481 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     3541 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     3601 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     3661 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     3721 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     3781 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     3841 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     3901 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     3961 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4021 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4081 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4141 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4201 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4261 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4321 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4381 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4441 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4501 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4561 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4621 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4681 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4741 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4801 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4861 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4921 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4981 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5041 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5101 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5161 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5221 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5281 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5341 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5401 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5461 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5521 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5581 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5641 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5701 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5761 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5821 ttttgctggc cttttgctca catgt
//