LOCUS       pAAV_GFAP_Sypher3S_cyto 6783 bp ds-DNA     circular     29-OCT-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      23..611
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     repeat_region   673..813
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     misc_feature    828..2746
                     /label="GFAP"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    2759..4165
                     /label="Sypher3S"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
     polyA_signal    4202..4678
                     /label="hGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: human growth hormone polyadenylation signal"
     repeat_region   4718..4858
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     rep_origin      4933..5388
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        5670..5774
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             5775..5843
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             5844..6635
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa
      721 agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag
      781 agggagtggc caactccatc actaggggtt cctgcggccg cacgcgtCTT AAGGCTGGGA
      841 GGTTGAGGCT GCAGTGAGTC GTGGTTGCGC CACTGCACTC CAGCCTGGGC AACAGTGAGA
      901 CCCTGTCTCA AAAGACAAAA AAAAAAAAAA AAAAAAAAAG AACATATCCT GGTGTGGAGT
      961 AGGGGACGCT GCTCTGACAG AGGCTCGGGG GCCTGAGCTG GCTCTGTGAG CTGGGGAGGA
     1021 GGCAGACAGC CAGGCCTTGT CTGCAAGCAG ACCTGGCAGC ATTGGGCTGG CCGCCCCCCA
     1081 GGGCCTCCTC TTCATGCCCA GTGAATGACT CACCTTGGCA CAGACACAAT GTTCGGGGTG
     1141 GGCACAGTGC CTGCTTCCCG CCGCACCCCA GCCCCCCTCA AATGCCTTCC GAGAAGCCCA
     1201 TTGAGCAGGG GGCTTGCATT GCACCCCAGC CTGACAGCCT GGCATCTTGG GATAAAAGCA
     1261 GCACAGCCCC CTAGGGGCTG CCCTTGCTGT GTGGCGCCAC CGGCGGTGGA GAACAAGGCT
     1321 CTATTCAGCC TGTGCCCAGG AAAGGGGATC AGGGGATGCC CAGGCATGGA CAGTGGGTGG
     1381 CAGGGGGGGA GAGGAGGGCT GTCTGCTTCC CAGAAGTCCA AGGACACAAA TGGGTGAGGG
     1441 GACTGGGCAG GGTTCTGACC CTGTGGGACC AGAGTGGAGG GCGTAGATGG ACCTGAAGTC
     1501 TCCAGGGACA ACAGGGCCCA GGTCTCAGGC TCCTAGTTGG GCCCAGTGGC TCCAGCGTTT
     1561 CCAAACCCAT CCATCCCCAG AGGTTCTTCC CATCTCTCCA GGCTGATGTG TGGGAACTCG
     1621 AGGAAATAAA TCTCCAGTGG GAGACGGAGG GGTGGCCAGG GAAACGGGGC GCTGCAGGAA
     1681 TAAAGACGAG CCAGCACAGC CAGCTCATGT GTAACGGCTT TGTGGAGCTG TCAAGGCCTG
     1741 GTCTCTGGGA GAGAGGCACA GGGAGGCCAG ACAAGGAAGG GGTGACCTGG AGGGACAGAT
     1801 CCAGGGGCTA AAGTCCTGAT AAGGCAAGAG AGTGCCGGCC CCCTCTTGCC CTATCAGGAC
     1861 CTCCACTGCC ACATAGAGGC CATGATTGAC CCTTAGACAA AGGGCTGGTG TCCAATCCCA
     1921 GCCCCCAGCC CCAGAACTCC AGGGAATGAA TGGGCAGAGA GCAGGAATGT GGGACATCTG
     1981 TGTTCAAGGG AAGGACTCCA GGAGTCTGCT GGGAATGAGG CCTAGTAGGA AATGAGGTGG
     2041 CCCTTGAGGG TACAGAACAG GTTCATTCTT CGCCAAATTC CCAGCACCTT GCAGGCACTT
     2101 ACAGCTGAGT GAGATAATGC CTGGGTTATG AAATCAAAAA GTTGGAAAGC AGGTCAGAGG
     2161 TCATCTGGTA CAGCCCTTCC TTCCCTTTTT TTTTTTTTTT TTTTGTGAGA CAAGGTCTCT
     2221 CTCTGTTGCC CAGGCTGGAG TGGCGCAAAC ACAGCTCACT GCAGCCTCAA CCTACTGGGC
     2281 TCAAGCAATC CTCCAGCCTC AGCCTCCCAA AGTGCTGGGA TTACAAGCAT GAGCCACCCC
     2341 ACTCAGCCCT TTCCTTCCTT TTTAATTGAT GCATAATAAT TGTAAGTATT CATCATGGTC
     2401 CAACCAACCC TTTCTTGACC CACCTTCCTA GAGAGAGGGT CCTCTTGCTT CAGCGGTCAG
     2461 GGCCCCAGAC CCATGGTCTG GCTCCAGGTA CCACCTGCCT CATGCAGGAG TTGGCGTGCC
     2521 CAGGAAGCTC TGCCTCTGGG CACAGTGACC TCAGTGGGGT GAGGGGAGCT CTCCCCATAG
     2581 CTGGGCTGCG GCCCAacccc accccctcag gctatgccag ggggtgttgc caggggcacc
     2641 cgggcatcgc cagtctagcc cactccttca taaagccctc gcatcccagg agcgagcaga
     2701 gccagagcag gttggagagg agacgcatca cctccgctgc tcgcGGgaat tcgccaccAT
     2761 GTCCGGACCG CTGCACATTG GTTTGATTCC CACAGTTGGA CCGTACCTGC TACCGCATAT
     2821 TATCCCTATG CTGCACCAGA CCTTTCCAAA GCTGGAAATG TATCTGCATG AAGCACAGAC
     2881 CCACCAGTTA CTGGCGCAAC TGGACAGCGG CAAACTCGAT TGCGTGATCC TCGCGCTGGT
     2941 GAAAGAGAGC GAAGCATTCA TTGAAGTGCC GTTGTTTGAT GAGCCAATGT TGCTGGCTAT
     3001 CTATGAAGAT CACCCGTGGG CGAACCGCGA ATGCGTACCG ATGGCCGATC TGGCAGGGGA
     3061 AAAACTGCTG ATGCTGGAAG ATGGTCACTC TTTGCGCGAT CAGGCAATGT CCGCCGGCTT
     3121 CAACAGCGAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGCCAACTT
     3181 CAAGATCCGC CACAACGTCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA
     3241 CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCTTCCAGTC
     3301 CGTCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCaGG AGTTCGTGAC
     3361 CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAC GTGGATGGCG GTAGCGGTGG
     3421 CACCGGCAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
     3481 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA
     3541 CGGCAAGCTG ACCCTGAAGC TGATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
     3601 CCTCGTGACC ACCCTCGGCT ACGGCCTGAA GTGCTTCGCC CGCTACCCCG ACCACATGAA
     3661 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT
     3721 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
     3781 GGTGAACCGC ATCGAGCTGA AGGGCATCGG CTTCAAGGAG GACGGCAACA TCCTGGGGCA
     3841 CAAGCTGGAG TACAACGGCA CCGGTTTCTG TTTTGAAGCC GGGGCGGATG AAGATACACA
     3901 CTTCCGCGCG ACCAGCCTGG AAACTCTGCG CAACATGGTG GCGGCAGGTA GCGGGATCAC
     3961 TTTACTGCCA GCGCTGGCTG TGCCGCCGGA GCGCAAACGC GATGGGGTTG TTTATCTGCC
     4021 GTGCATTAAG CCGGAACCAC GCCGCACTAT TGGCCTGGTT TATCGTCCTG GCTCACCGCT
     4081 GCGCAGCCGC TATGAGCAGC TGGCAGAGGC CATCCGCGCA AGAATGGATG GCCATTTCGA
     4141 TAAAGTTTTA AAACAGGCGG TTTAAaagct tgcctcgagc agcgctgctc gagagatcta
     4201 cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc
     4261 agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc
     4321 cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac
     4381 aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg
     4441 gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt
     4501 gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg
     4561 gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc
     4621 ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct
     4681 gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     4741 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     4801 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     4861 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     4921 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4981 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     5041 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     5101 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     5161 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5221 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     5281 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5341 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     5401 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     5461 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     5521 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     5581 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     5641 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5701 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5761 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5821 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5881 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5941 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6001 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6061 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6121 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6181 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6241 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6301 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6361 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6421 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6481 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6541 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6601 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6661 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6721 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6781 ccg
//