LOCUS       pAAV9_hSyn1_SypHer3s_mi 5798 bp ds-DNA     circular     30-OCT-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      283..965
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        1153..1456
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        1464..1911
                     /label="hSyn promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human synapsin I promoter; confers neuron-specific expression (Kügler et al., 2003)"
     CDS             1927..1998
                     /label="COX8 presequence"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="EC_number: codon_start: 1 gene: product: mitochondrial presequence of human cytochrome c oxidase subunit VIII (Rizutto et al., 1989) protein_id: transl_table: 1 translation: SVLTPLLLRGLTGSARRLPVPRAK"
     misc_feature    1999..2019
                     /label="linker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             2020..2091
                     /label="COX8 presequence"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="EC_number: codon_start: 1 gene: product: mitochondrial presequence of human cytochrome c oxidase subunit VIII (Rizutto et al., 1989) protein_id: transl_table: 1 translation: SVLTPLLLRGLTGSARRLPVPRAK"
     misc_feature    2113..3516
                     /label="SypHer3s"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     polyA_signal    3553..4029
                     /label="hGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: human growth hormone polyadenylation signal"
     repeat_region   4069..4209
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     primer_bind     complement(4756..4775)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     4875..4897
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4935..4953)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward primer"
     promoter        5021..5125
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             5126..5194
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             5324..185
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG
       61 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC
      121 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC
      181 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT
      241 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT
      301 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT
      361 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG
      421 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA
      481 GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA
      541 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG
      601 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG
      661 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT
      721 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA
      781 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC
      841 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG
      901 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG
      961 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTCC TGCAGGCAGC
     1021 TGCGCGCTCG CTCGCTCACT GAGGCCGCCC GGGCGTCGGG CGACCTTTGG TCGCCCGGCC
     1081 TCAGTGAGCG AGCGAGCGCG CAGAGAGGGA GTGGCCAACT CCATCACTAG GGGTTCCTGC
     1141 GGCCGCACGC GTCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA
     1201 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT
     1261 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT
     1321 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA
     1381 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT
     1441 CATCGCTATT ACCATGGCTC GAGAGTGCAA GTGGGTTTTA GGACCAGGAT GAGGCGGGGT
     1501 GGGGGTGCCT ACCTGACGAC CGACCCCGAC CCACTGGACA AGCACCCAAC CCCCATTCCC
     1561 CAAATTGCGC ATCCCCTATC AGAGAGGGGG AGGGGAAACA GGATGCGGCG AGGCGCGTGC
     1621 GCACTGCCAG CTTCAGCACC GCGGACAGTG CCTTCGCCCC CGCCTGGCGG CGCGCGCCAC
     1681 CGCCGCCTCA GCACTGAAGG CGCGCTGACG TCACTCGCCG GTCCCCCGCA AACTCCCCTT
     1741 CCCGGCCACC TTGGTCGCGT CCGCGCCGCC GCCGGCCCAG CCGGACCGCA CCACGCGAGG
     1801 CGCGAGATAG GGGGGCACGG GCGCGACCAT CTGCGCTGCG GCGCCGGCGA CTCAGCGCTG
     1861 CCTCAGTCTG CGGTGGGCAG CGGAGGAGTC GTGTCGTGCC TGAGAGCGCA GGAATTCGCC
     1921 ACCATGTCCG TCCTGACGCC GCTGCTGCTG CGGGGCTTGA CAGGCTCGGC CCGGCGGCTC
     1981 CCAGTGCCGC GCGCCAAGAT CCATTCGTTG GGGGATCTGT CCGTCCTGAC GCCGCTGCTG
     2041 CTGCGGGGCT TGACAGGCTC GGCCCGGCGG CTCCCAGTGC CGCGCGCCAA GATCCATTCG
     2101 TTGGGGGATC CATCCGGACC GCTGCACATT GGTTTGATTC CCACAGTTGG ACCGTACCTG
     2161 CTACCGCATA TTATCCCTAT GCTGCACCAG ACCTTTCCAA AGCTGGAAAT GTATCTGCAT
     2221 GAAGCACAGA CCCACCAGTT ACTGGCGCAA CTGGACAGCG GCAAACTCGA TTGCGTGATC
     2281 CTCGCGCTGG TGAAAGAGAG CGAAGCATTC ATTGAAGTGC CGTTGTTTGA TGAGCCAATG
     2341 TTGCTGGCTA TCTATGAAGA TCACCCGTGG GCGAACCGCG AATGCGTACC GATGGCCGAT
     2401 CTGGCAGGGG AAAAACTGCT GATGCTGGAA GATGGTCACT CTTTGCGCGA TCAGGCAATG
     2461 TCCGCCGGCT TCAACAGCGA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
     2521 AAGGCCAACT TCAAGATCCG CCACAACGTC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
     2581 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
     2641 AGCTTCCAGT CCGTCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCAG
     2701 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA CGTGGATGGC
     2761 GGTAGCGGTG GCACCGGCAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG
     2821 GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC
     2881 GATGCCACCT ACGGCAAGCT GACCCTGAAG CTGATCTGCA CCACCGGCAA GCTGCCCGTG
     2941 CCCTGGCCCA CCCTCGTGAC CACCCTCGGC TACGGCCTGA AGTGCTTCGC CCGCTACCCC
     3001 GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG
     3061 CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG
     3121 GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG GCTTCAAGGA GGACGGCAAC
     3181 ATCCTGGGGC ACAAGCTGGA GTACAACGGC ACCGGTTTCT GTTTTGAAGC CGGGGCGGAT
     3241 GAAGATACAC ACTTCCGCGC GACCAGCCTG GAAACTCTGC GCAACATGGT GGCGGCAGGT
     3301 AGCGGGATCA CTTTACTGCC AGCGCTGGCT GTGCCGCCGG AGCGCAAACG CGATGGGGTT
     3361 GTTTATCTGC CGTGCATTAA GCCGGAACCA CGCCGCACTA TTGGCCTGGT TTATCGTCCT
     3421 GGCTCACCGC TGCGCAGCCG CTATGAGCAG CTGGCAGAGG CCATCCGCGC AAGAATGGAT
     3481 GGCCATTTCG ATAAAGTTTT AAAACAGGCG GTTTAAAAGC TTGCCTCGAG CAGCGCTGCT
     3541 CGAGAGATCT ACGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA
     3601 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT
     3661 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA
     3721 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG
     3781 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG
     3841 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT
     3901 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG
     3961 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC
     4021 CCTGTCCTTC TGATTTTGTA GGTAACCACG TGCGGACCGA GCGGCCGCAG GAACCCCTAG
     4081 TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA
     4141 AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCT
     4201 GCCTGCAGGG GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC
     4261 GCATACGTCA AAGCAACCAT AGTACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT
     4321 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC TTAGCGCCCG CTCCTTTCGC
     4381 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG
     4441 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTT
     4501 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT
     4561 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACTCTAT
     4621 CTCGGGCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGTCTATT GGTTAAAAAA
     4681 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTT
     4741 ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC
     4801 GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA
     4861 AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG
     4921 CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT
     4981 GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT
     5041 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT
     5101 TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC
     5161 CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA
     5221 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG
     5281 TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT
     5341 TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG
     5401 CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC
     5461 GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC
     5521 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA
     5581 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC
     5641 AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT
     5701 AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA
     5761 TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGG
//