LOCUS       pNA-011                 2601 bp ds-DNA     circular     15-APR-2025
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: 2 pCDF-MCS.gbk.txt
FEATURES             Location/Qualifiers
     misc_feature    184..189
                     /label="EcoRI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    190..219
                     /label="Linker_14"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    220..225
                     /label="SacI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    226..237
                     /label="Spacer 8"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    238..243
                     /label="XhoI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    244..273
                     /label="Linker_0"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    274..279
                     /label="NcoI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    280..369
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    370..569
                     /label="2017mar28_200bpSpacer1* (C->G change to remove AGCAGY)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    492..492
                     /label="C->G change to remove AGCAGY"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    570..575
                     /label="HindIII"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            complement(991..1779)
                     /label="SpecR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(1919..2601)
                     /label="cloned fragment (expanded ori ???)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(1937..2525)
                     /label="pUC ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 ttttctactg aaccgctcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
       61 gtcagcccca tacgatataa gttgtaattc tcatgttagt catgccccgc gcccaccgga
      121 aggagctgac tgggttgCTC CTAGGATGCA GTGACTCGca catttccccg aaaagtgcca
      181 cctGAATTCA TAGGATAGAT TCTGGAAACT TTACCGTCCG AGCTCAGGCT TACCTTACTC
      241 GAGCAATAAA CAGTTGATAG GGCTTCTCCG TTACCATGGT TCAGCCAAAA AACTTAAGAC
      301 CGCCGGTCTT GTCCACTACC TTGCAGTAAT GCGGTGGACA GGATCGGCGG TTTTCTTTTC
      361 TCTTCTCAAT TCTTCTGACC TGTAACGAAT AATAGATAGT AAAGTAGTCT CCGATTGAGT
      421 TTTCTCTGCC GAGTCCCACC CAGTTCTGTG ATTTCAGTAA GTTGGTAATT GATACACTGT
      481 TGCGAGAACT GgTGCCTGGT AGTAGATAGG TTGTTATTGA GTAAGAAGGT AAAGTGAACG
      541 AAATCCCTGA AACTGAGACT GTAGAAAATA AGCTTTGCTG CCACCGCTGA GCAATAACTA
      601 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCCAGG CATCAAATAA
      661 AACGAAAGGC TCAGTCGGAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG
      721 CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGTGAAG CAACGGCCCG
      781 GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAACTAAG CAGAAGGCCA
      841 TCCTGACGGA TGGCCTTTTT GCGTTTCAGA TCTaccggta aaccagcaat agacataagc
      901 ggctatttaa cgaccctgcc ctgaaccgac gaccgggtca tcgtggccgg atcttgcggc
      961 ccctcggctt gaacgaattg ttagacatta tttgccgact accttggtga tctcgccttt
     1021 cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg
     1081 tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
     1141 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
     1201 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
     1261 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
     1321 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
     1381 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
     1441 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
     1501 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
     1561 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
     1621 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
     1681 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
     1741 tagttgagtc gatacttcgg cgatcaccgc ttccctcata ctcttccttt ttcaatatta
     1801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     1861 aaataaacaa atagctagct cactcggtcg ctacgctccg ggcgtgagac tgcggcggGG
     1921 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC
     1981 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
     2041 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT
     2101 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG
     2161 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
     2221 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC
     2281 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT
     2341 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC
     2401 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA
     2461 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
     2521 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC
     2581 GTTAAGGGAT TTTGGTCATG A
//