LOCUS       pNC-011                 2440 bp ds-DNA     circular     15-APR-2025
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: 2 pCDF-MCS.gbk.txt
FEATURES             Location/Qualifiers
     misc_feature    184..189
                     /label="EcoRI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    190..217
                     /label="Truncated (3') Spacer 2.5"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    218..223
                     /label="SalI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    224..235
                     /label="Spacer 5"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    236..241
                     /label="AatII"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    242..271
                     /label="Linker_11"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    272..279
                     /label="NotI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    280..336
                     /label="ECK120033737 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    337..381
                     /label="Linker 8"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    382..387
                     /label="KasI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    388..399
                     /label="Spacer 15"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    400..406
                     /label="BbvCI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    407..414
                     /label="AscI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            complement(830..1618)
                     /label="SpecR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(1758..2440)
                     /label="cloned fragment (expanded ori ???)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(1776..2364)
                     /label="pUC ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 ttttctactg aaccgctcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
       61 gtcagcccca tacgatataa gttgtaattc tcatgttagt catgccccgc gcccaccgga
      121 aggagctgac tgggttgCTC CTAGGATGCA GTGACTCGca catttccccg aaaagtgcca
      181 cctGAATTCA GAAGTATTGG TAATCGTTGA AAACTCAGTC GACGCACTTA CTGAAGACGT
      241 CCTATTACAC TCGTCGTTGG AAACTGAAGA TGCGGCCGCg gaaacacagA AAAAAGCCCG
      301 CACCTGACAG TGCGGGCTTT TTTTTTcgac caaaggTAGC GAACGACGAG TCACTGTTGA
      361 GGATAAATAC TTTCTCTACT AGGCGCCTGT TACACAGGTC CTCAGCGGCG CGCCTGCTGC
      421 CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT
      481 TTTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGGAAG ACTGGGCCTT TCGTTTTATC
      541 TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC CGCCGGGAGC GGATTTGAAC
      601 GTTGTGAAGC AACGGCCCGG AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGCAT
      661 CAAACTAAGC AGAAGGCCAT CCTGACGGAT GGCCTTTTTG CGTTTCAGAT CTaccggtaa
      721 accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcat
      781 cgtggccgga tcttgcggcc cctcggcttg aacgaattgt tagacattat ttgccgacta
      841 ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg
      901 ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat
      961 actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc
     1021 cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag
     1081 cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct
     1141 gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt
     1201 ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac
     1261 ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac
     1321 gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc
     1381 tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt
     1441 catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc
     1501 catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga
     1561 tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatac
     1621 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     1681 tatttgaatg tatttagaaa aataaacaaa tagctagctc actcggtcgc tacgctccgg
     1741 gcgtgagact gcggcggGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
     1801 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
     1861 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
     1921 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC
     1981 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
     2041 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
     2101 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
     2161 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA
     2221 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT
     2281 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
     2341 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     2401 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
//