LOCUS       pEND-11Y                5522 bp ds-DNA     circular     15-APR-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        274..302
                     /label="P(BAD) promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    315..320
                     /label="EcoRI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    321..350
                     /label="Linker_14"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    351..356
                     /label="SacI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    357..368
                     /label="Spacer 8"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    369..374
                     /label="XhoI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    375..404
                     /label="Linker_0"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    405..410
                     /label="NcoI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    411..500
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    501..700
                     /label="2017mar28_200bpSpacer1* (C->G change to remove AGCAGY)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    623..623
                     /label="C->G change to remove AGCAGY"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    701..706
                     /label="HindIII"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    707..721
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    722..756
                     /label="P(BBa_J23100)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    757..771
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    772..801
                     /label="VL2017mar22_24"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    802..816
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(817..819)
                     /label="PAM"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(817..839)
                     /label="bssgRNA-1 _ ANTISENSE is identical (orthogonal set from Didovyk et al 2015)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(820..839)
                     /label="orthogonal bssgRNA-1 _ ANTISENSE is identical (Didovyk et al 2015)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    840..854
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    855..884
                     /label="VL2017mar22_22"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    885..899
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    900..919
                     /label="Csy4 cut site - Tsai et al 2014"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    905..919
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    919..919
                     /label="Csy4 cuts after this G"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    920..953
                     /label="RBS"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     misc_feature    941..946
                     /label="Shine-Dalgarno ?"
                     /ApEinfo_revcolor="#fdc68a"
                     /ApEinfo_fwdcolor="#fdc68a"
     gene            954..1667
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             954..1733
                     /label="Translation 954-1733"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKMSRRNTDAITIHSILDWIED**"
     misc_feature    1668..1733
                     /label="MarAn20 degradation tag (C-term)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1734..1748
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1749..1754
                     /label="BamHI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1755..1784
                     /label="Linker_10"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1785..1792
                     /label="SbfI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1793..1853
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1854..2053
                     /label="2018mar28_200bpSpacer2.5* (A->T change to remove AGCAGY)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1915..1915
                     /label="A->T change to remove AGCAGY"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2054..2059
                     /label="SalI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2060..2074
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2075..2109
                     /label="P(BBa_J23150) - weak"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2110..2124
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2125..2154
                     /label="VL2017mar22_19"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2155..2169
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(2170..2172)
                     /label="PAM"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(2170..2192)
                     /label="bssgRNA-2 _ ANTISENSE is identical (orthogonal set from Didovyk et al 2015)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(2173..2192)
                     /label="orthogonal bssgRNA-2 _ ANTISENSE is identical (Didovyk et al 2015)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2193..2207
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2208..2237
                     /label="VL2017mar22_17"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2238..2252
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2253..2272
                     /label="Csy4 cut site - Tsai et al 2014"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2258..2272
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2272..2272
                     /label="Csy4 cuts after this G"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2273..2292
                     /label="orthogonal gRNA-1 (Didovyk et al 2015)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2273..2378
                     /label="sgRNA-1 (orthogonal set from Didovyk et al 2015)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2293..2378
                     /label="sgRNA(constant) optimised (Dang et al 2015) without terminator"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2297..2297
                     /label="T->C change to optimise (Dang et al 2015)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2305..2309
                     /label="extended hairpin (Dang et al 2015)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2310..2313
                     /label="loop"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(2314..2318)
                     /label="extended hairpin (Dang et al 2015)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2323..2325
                     /label="not annealing"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(2328..2328)
                     /label="T->C change to optimise (Dang et al 2015)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2379..2398
                     /label="Csy4 cut site - Tsai et al 2014"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2384..2398
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2398..2398
                     /label="Csy4 cuts after this G"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2399..2413
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2414..2419
                     /label="AatII"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2420..2449
                     /label="Linker_11"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2450..2457
                     /label="NotI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2458..2514
                     /label="ECK120033737 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2515..2559
                     /label="Linker 8"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2560..2565
                     /label="KasI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2566..2577
                     /label="Spacer 15"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2578..2584
                     /label="BbvCI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2585..2592
                     /label="AscI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            complement(2933..3748)
                     /label="KanR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     oriT            3858..4493
                     /label="ColA ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            complement(4644..5522)
                     /label="araC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 ACTTTTCATA CTCCCGCCAT TCAGAGAAGA AACCAATTGT CCATATTGCA TCAGACATTG
       61 CCGTCACTGC GTCTTTTACT GGCTCTTCTC GCTAACCAAA CCGGTAACCC CGCTTATTAA
      121 AAGCATTCTG TAACAAAGCG GGACCAAAGC CATGACAAAA ACGCGTAACA AAAGTGTCTA
      181 TAATCACGGC AGAAAAGTCC ACATTGATTA TTTGCACGGC GTCACACTTT GCTATGCCAT
      241 AGCATTTTTA TCCATAAGAT TAGCGGtTCC TACCTGACGC TTTTTATCGC AACTCTCTAC
      301 TGTTTCTCCA TACCGAATTC ATAGGATAGA TTCTGGAAAC TTTACCGTCC GAGCTCAGGC
      361 TTACCTTACT CGAGCAATAA ACAGTTGATA GGGCTTCTCC GTTACCATGG TTCAGCCAAA
      421 AAACTTAAGA CCGCCGGTCT TGTCCACTAC CTTGCAGTAA TGCGGTGGAC AGGATCGGCG
      481 GTTTTCTTTT CTCTTCTCAA TTCTTCTGAC CTGTAACGAA TAATAGATAG TAAAGTAGTC
      541 TCCGATTGAG TTTTCTCTGC CGAGTCCCAC CCAGTTCTGT GATTTCAGTA AGTTGGTAAT
      601 TGATACACTG TTGCGAGAAC TGgTGCCTGG TAGTAGATAG GTTGTTATTG AGTAAGAAGG
      661 TAAAGTGAAC GAAATCCCTG AAACTGAGAC TGTAGAAAAT AAGCTTCAGC CTGCGGTCCG
      721 Gttgacggct agctcagtcc taggtacagt gctagcTCGC TGGGACGCCC GAGATAGCCG
      781 TTACACAGGT GACACTTATT TCAGCCTGCG GTCCGGccaC ACTAGAGTCT AGCTTGAGAT
      841 CGCTGGGACG CCCGTAGAAC TCTGTGAGGA TAAAGTCTCC CTTACAGCCT GCGGTCCGGG
      901 TTCACTGCCG TATAGGCAGT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGCGTA
      961 AAGGCGAAGA ACTGTTTACC GGTGTGGTTC CGATTCTGGT GGAACTGGAC GGCGATGTTA
     1021 ATGGTCATAA ATTCAGTGTT CGCGGCGAAG GTGAAGGCGA TGCGACGAAC GGCAAACTGA
     1081 CCCTGAAATT TATCTGCACC ACGGGTAAAC TGCCGGTCCC GTGGCCGACG CTGGTGACCA
     1141 CGCTGACCTA TGGCGTTCAA TGTTTTGCGC GTTACCCGGA TCACATGAAA CAGCACGACT
     1201 TTTTCAAATC GGCCATGCCG GAAGGCTATG TGCAGGAACG TACGATTAGC TTTAAAGACG
     1261 ATGGTACGTA TAAAACCCGC GCGGAAGTGA AATTCGAAGG CGATACCCTG GTTAACCGTA
     1321 TCGAACTGAA AGGTATCGAT TTCAAAGAAG ACGGCAATAT TCTGGGTCAT AAACTGGAAT
     1381 ATAACTTCAA TTCCCACAAC GTGTACATCA CCGCGGATAA ACAGAAAAAC GGCATTAAAG
     1441 CCAATTTCAA AATCCGCCAT AATGTGGAAG ATGGTAGCGT TCAGCTGGCC GACCACTATC
     1501 AGCAAAACAC GCCGATTGGT GATGGCCCGG TCCTGCTGCC GGACAATCAC TACCTGAGTA
     1561 CCCAGTCCGT GCTGTCAAAA GATCCGAACG AAAAACGTGA CCACATGGTC CTGCTGGAAT
     1621 TTGTGACGGC TGCGGGTATC ACCCACGGCA TGGACGAACT GTATAAAATG TCCCGCCGTA
     1681 ATACTGACGC CATCACAATC CACAGCATCC TGGATTGGAT TGAAGACTAA TAATCGCTGG
     1741 GACGCCCGGG ATCCAAGAGA TTTCTACACG ATTGAGCACT GTCTCCTGCA GGCTCGGTAC
     1801 CAAATTCCAG AAAAGAGGCC TCCCGAAAGG GGGGCCTTTT TTCGTTTTGG TCCTAATAGA
     1861 TAAAGGATAG GTCTGGTAGT GTTGTTCGTT CTCGCAGGTA AATCAATAAT ACTCtGCAGT
     1921 TCCGTAGACT TTTCAGTGGG ACAGGGTAGC GATAACAGAT AGATTGTAAT AAGACACAGT
     1981 AGGTGCTCGT AGTTGCGTGA AGAGAACCGC TCAGGAAATC CAGTCAGAAG TATTGGTAAT
     2041 CGTTGAAAAC TCAGTCGACC AGCCTGCGGT CCGGTTTACG GCTAGCTCAG TCCTAGGTAT
     2101 TATGCTAGCT CGCTGGGACG CCCGTGAACA GTTGCTCTGA TTGAAACCAC GATTCAGCCT
     2161 GCGGTCCGGc caAGTACTTA GTACACACTG ATTCGCTGGG ACGCCCGGTA GTGCTTATCA
     2221 GACCCAATAC TGTTGAACAG CCTGCGGTCC GGGTTCACTG CCGTATAGGC AGTCTCAAGC
     2281 TAGACTCTAG TGGTTTcAGA GCTATGCTGG AAACAGCATA GCAAGTTgAA ATAAGGCTAG
     2341 TCCGTTATCA ACTTGAAAAA GTGGCACCGA GTCGGTGCGT TCACTGCCGT ATAGGCAGTC
     2401 GCTGGGACGC CCGGACGTCC TATTACACTC GTCGTTGGAA ACTGAAGATG CGGCCGCgga
     2461 aacacagAAA AAAGCCCGCA CCTGACAGTG CGGGCTTTTT TTTTcgacca aaggTAGCGA
     2521 ACGACGAGTC ACTGTTGAGG ATAAATACTT TCTCTACTAG GCGCCTGTTA CACAGGTCCT
     2581 CAGCGGCGCG CCTTTGTCGG TGAACGCTCT CCTGAGTAGG ACAAATCCGC CGGGAGCGGA
     2641 TTTGAACGTT GTGAAGCAAC GGCCCGGAGG GTGGCGGGCA GGACGCCCGC CATAAACTGC
     2701 CAGGCATCAA ACTAAGCAGA AGGCCATCCT GACGGATGGC CTTTTTGCGT TTCAGATCTA
     2761 CCGGTaaacc agcaatagac ataagcggct atttaacgac cctgccctga accgacgaca
     2821 agctgacgac cgggtctccg caagtggcac ttttcgggga aatgtgcgcg gaacccctat
     2881 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa
     2941 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     3001 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     3061 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     3121 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     3181 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac
     3241 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     3301 cgagacgaaa tacgcggtcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     3361 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     3421 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag
     3481 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     3541 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     3601 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     3661 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc
     3721 aagacgtttc ccgttgaata tggctcatac tcttcctttt tcaatattat tgaagcattt
     3781 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3841 taggcatgct agcgcagaaa cgtcctagaa gatgccagga ggatacttag cagagagaca
     3901 ataaggccgg agcgaagccg tttttccata ggctccgccc ccctgacgaa catcacgaaa
     3961 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4021 cccctgatgg ctccctcttg cgctctcctg ttcccgtcct gcggcgtccg tgttgtggtg
     4081 gaggctttac ccaaatcacc acgtcccgtt ccgtgtagac agttcgctcc aagctgggct
     4141 gtgtgcaaga accccccgtt cagcccgact gctgcgcctt atccggtaac tatcatcttg
     4201 agtccaaccc ggaaagacac gacaaaacgc cactggcagc agccattggt aactgagaat
     4261 tagtggattt agatatcgag agtcttgaag tggtggccta acagaggcta cactgaaagg
     4321 acagtatttg gtatctgcgc tccactaaag ccagttacca ggttaagcag ttccccaact
     4381 gacttaacct tcgatcaaac cgcctcccca ggcggttttt tcgtttacag agcaggagat
     4441 tacgacgatc gtaaaaggat ctcaagaaga tcctttacgg attcccgaca ccatcactct
     4501 agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata
     4561 agttgtaatt ctcatgttag tcatgccccg cgcccaccgg aaggagctga ctgggttgCT
     4621 CCTAgGGTCT GATTCGTTAC CAATTATGAC AACTTGACGG CTACATCATT CACTTTTTCT
     4681 TCACAACCGG CACGGAACTC GCTCGGGCTG GCCCCGGTGC ATTTTTTAAA TACCCGCGAG
     4741 AAATAGAGTT GATCGTCAAA ACCAACATTG CGACCGACGG TGGCGATAGG CATCCGGGTG
     4801 GTGCTCAAAA GCAGCTTCGC CTGGCTGATA CGTTGGTCCT CGCGCCAGCT TAAGACGCTA
     4861 ATCCCTAACT GCTGGCGGAA AAGATGTGAC AGACGCGACG GCGACAAGCA AACATGCTGT
     4921 GCGACGCTGG CGATATCAAA ATTGCTGTCT GCCAGGTGAT CGCTGATGTA CTGACAAGCC
     4981 TCGCGTACCC GATTATCCAT CGGTGGATGG AGCGACTCGT TAATCGCTTC CATGCGCCGC
     5041 AGTAACAATT GCTCAAGCAG ATTTATCGCC AGCAGCTCCG AATAGCGCCC TTCCCCTTGC
     5101 CCGGCGTTAA TGATTTGCCC AAACAGGTCG CTGAAATGCG GCTGGTGCGC TTCATCCGGG
     5161 CGAAAGAACC CCGTATTGGC AAATATTGAC GGCCAGTTAA GCCATTCATG CCAGTAGGCG
     5221 CGCGGACGAA AGTAAACCCA CTGGTGATAC CATTCGCGAG CCTCCGGATG ACGACCGTAG
     5281 TGATGAATCT CTCCTGGCGG GAACAGCAAA ATATCACCCG GTCGGCAAAC AAATTCTCGT
     5341 CCCTGATTTT TCACCACCCC CTGACCGCGA ATGGTGAGAT TGAGAATATA ACCTTTCATT
     5401 CCCAGCGGTC GGTCGATAAA AAAATCGAGA TAACCGTTGG CCTCAATCGG CGTTAAACCC
     5461 GCCACCAGAT GGGCATTAAA CGAGTATCCC GGCAGCAGGG GATCATTTTG CGCTTCAGCC
     5521 AT
//