LOCUS       pEND-Z11                5344 bp ds-DNA     circular     15-APR-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        274..302
                     /label="P(BAD) promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    315..320
                     /label="EcoRI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    321..350
                     /label="VL2017mar22_14"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    351..356
                     /label="SacI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    357..371
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    372..391
                     /label="Csy4 cut site - Tsai et al 2014"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    377..391
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    391..391
                     /label="Csy4 cuts after this G"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    392..411
                     /label="sgRNA-2 (orthogonal set from Didovyk et al 2015)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    392..497
                     /label="sgRNA-2 (orthogonal set from Didovyk et al 2015)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    412..497
                     /label="sgRNA(constant) optimised (Dang et al 2015) without terminator"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    416..416
                     /label="T->C change to optimise (Dang et al 2015)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    424..428
                     /label="extended hairpin (Dang et al 2015)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    429..432
                     /label="loop"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(433..437)
                     /label="extended hairpin (Dang et al 2015)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    442..444
                     /label="not annealing"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(447..447)
                     /label="T->C change to optimise (Dang et al 2015)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    498..517
                     /label="Csy4 cut site - Tsai et al 2014"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    503..517
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    517..517
                     /label="Csy4 cuts after this G"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    518..532
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    533..538
                     /label="XhoI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    539..568
                     /label="VL2017mar22_0"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    569..574
                     /label="NcoI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    575..664
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    665..864
                     /label="2017mar28_200bpSpacer1* (C->G change to remove AGCAGY)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    787..787
                     /label="C->G change to remove AGCAGY"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    865..870
                     /label="HindIII"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    871..885
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    886..920
                     /label="P(BBa_J23100)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    921..935
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    936..965
                     /label="VL2017mar22_24"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    966..980
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(981..983)
                     /label="PAM"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(981..1003)
                     /label="bssgRNA-1 _ ANTISENSE is identical (orthogonal set from Didovyk et al 2015)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(984..1003)
                     /label="orthogonal bssgRNA-1 _ ANTISENSE is identical (Didovyk et al 2015)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1004..1018
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1019..1048
                     /label="VL2017mar22_22"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1049..1063
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1064..1083
                     /label="Csy4 cut site - Tsai et al 2014"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1069..1083
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1083..1083
                     /label="Csy4 cuts after this G"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1084..1117
                     /label="RBS"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     misc_feature    1105..1110
                     /label="Shine-Dalgarno ?"
                     /ApEinfo_revcolor="#fdc68a"
                     /ApEinfo_fwdcolor="#fdc68a"
     gene            1118..1831
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1118..1897
                     /label="Translation 1118-1897"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKMSRRNTDAITIHSILDWIED**"
     misc_feature    1832..1897
                     /label="MarAn20 degradation tag (C-term)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1898..1912
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1913..1918
                     /label="BamHI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1919..1948
                     /label="Linker_10"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1949..1956
                     /label="SbfI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1957..2017
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2018..2217
                     /label="2018mar28_200bpSpacer2.5* (A->T change to remove AGCAGY)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2079..2079
                     /label="A->T change to remove AGCAGY"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2218..2223
                     /label="SalI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2224..2235
                     /label="Spacer 5"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2236..2241
                     /label="AatII"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2242..2271
                     /label="Linker_11"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2272..2279
                     /label="NotI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2280..2336
                     /label="ECK120033737 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2337..2381
                     /label="Linker 8"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2382..2387
                     /label="KasI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2388..2399
                     /label="Spacer 15"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2400..2406
                     /label="BbvCI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2407..2414
                     /label="AscI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            complement(2755..3570)
                     /label="KanR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     oriT            3680..4315
                     /label="ColA ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            complement(4466..5344)
                     /label="araC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 ACTTTTCATA CTCCCGCCAT TCAGAGAAGA AACCAATTGT CCATATTGCA TCAGACATTG
       61 CCGTCACTGC GTCTTTTACT GGCTCTTCTC GCTAACCAAA CCGGTAACCC CGCTTATTAA
      121 AAGCATTCTG TAACAAAGCG GGACCAAAGC CATGACAAAA ACGCGTAACA AAAGTGTCTA
      181 TAATCACGGC AGAAAAGTCC ACATTGATTA TTTGCACGGC GTCACACTTT GCTATGCCAT
      241 AGCATTTTTA TCCATAAGAT TAGCGGtTCC TACCTGACGC TTTTTATCGC AACTCTCTAC
      301 TGTTTCTCCA TACCGAATTC ATAGGATAGA TTCTGGAAAC TTTACCGTCC GAGCTCCAGC
      361 CTGCGGTCCG GGTTCACTGC CGTATAGGCA GATCAGTGTG TACTAAGTAC TGTTTcAGAG
      421 CTATGCTGGA AACAGCATAG CAAGTTgAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG
      481 TGGCACCGAG TCGGTGCGTT CACTGCCGTA TAGGCAGTCG CTGGGACGCC CGCTCGAGCA
      541 ATAAACAGTT GATAGGGCTT CTCCGTTACC ATGGTTCAGC CAAAAAACTT AAGACCGCCG
      601 GTCTTGTCCA CTACCTTGCA GTAATGCGGT GGACAGGATC GGCGGTTTTC TTTTCTCTTC
      661 TCAATTCTTC TGACCTGTAA CGAATAATAG ATAGTAAAGT AGTCTCCGAT TGAGTTTTCT
      721 CTGCCGAGTC CCACCCAGTT CTGTGATTTC AGTAAGTTGG TAATTGATAC ACTGTTGCGA
      781 GAACTGgTGC CTGGTAGTAG ATAGGTTGTT ATTGAGTAAG AAGGTAAAGT GAACGAAATC
      841 CCTGAAACTG AGACTGTAGA AAATAAGCTT CAGCCTGCGG TCCGGttgac ggctagctca
      901 gtcctaggta cagtgctagc TCGCTGGGAC GCCCGAGATA GCCGTTACAC AGGTGACACT
      961 TATTTCAGCC TGCGGTCCGG ccaCACTAGA GTCTAGCTTG AGATCGCTGG GACGCCCGTA
     1021 GAACTCTGTG AGGATAAAGT CTCCCTTACA GCCTGCGGTC CGGGTTCACT GCCGTATAGG
     1081 CAGTAATTTT GTTTAACTTT AAGAAGGAGA TATACATATG CGTAAAGGCG AAGAACTGTT
     1141 TACCGGTGTG GTTCCGATTC TGGTGGAACT GGACGGCGAT GTTAATGGTC ATAAATTCAG
     1201 TGTTCGCGGC GAAGGTGAAG GCGATGCGAC GAACGGCAAA CTGACCCTGA AATTTATCTG
     1261 CACCACGGGT AAACTGCCGG TCCCGTGGCC GACGCTGGTG ACCACGCTGA CCTATGGCGT
     1321 TCAATGTTTT GCGCGTTACC CGGATCACAT GAAACAGCAC GACTTTTTCA AATCGGCCAT
     1381 GCCGGAAGGC TATGTGCAGG AACGTACGAT TAGCTTTAAA GACGATGGTA CGTATAAAAC
     1441 CCGCGCGGAA GTGAAATTCG AAGGCGATAC CCTGGTTAAC CGTATCGAAC TGAAAGGTAT
     1501 CGATTTCAAA GAAGACGGCA ATATTCTGGG TCATAAACTG GAATATAACT TCAATTCCCA
     1561 CAACGTGTAC ATCACCGCGG ATAAACAGAA AAACGGCATT AAAGCCAATT TCAAAATCCG
     1621 CCATAATGTG GAAGATGGTA GCGTTCAGCT GGCCGACCAC TATCAGCAAA ACACGCCGAT
     1681 TGGTGATGGC CCGGTCCTGC TGCCGGACAA TCACTACCTG AGTACCCAGT CCGTGCTGTC
     1741 AAAAGATCCG AACGAAAAAC GTGACCACAT GGTCCTGCTG GAATTTGTGA CGGCTGCGGG
     1801 TATCACCCAC GGCATGGACG AACTGTATAA AATGTCCCGC CGTAATACTG ACGCCATCAC
     1861 AATCCACAGC ATCCTGGATT GGATTGAAGA CTAATAATCG CTGGGACGCC CGGGATCCAA
     1921 GAGATTTCTA CACGATTGAG CACTGTCTCC TGCAGGCTCG GTACCAAATT CCAGAAAAGA
     1981 GGCCTCCCGA AAGGGGGGCC TTTTTTCGTT TTGGTCCTAA TAGATAAAGG ATAGGTCTGG
     2041 TAGTGTTGTT CGTTCTCGCA GGTAAATCAA TAATACTCtG CAGTTCCGTA GACTTTTCAG
     2101 TGGGACAGGG TAGCGATAAC AGATAGATTG TAATAAGACA CAGTAGGTGC TCGTAGTTGC
     2161 GTGAAGAGAA CCGCTCAGGA AATCCAGTCA GAAGTATTGG TAATCGTTGA AAACTCAGTC
     2221 GACGCACTTA CTGAAGACGT CCTATTACAC TCGTCGTTGG AAACTGAAGA TGCGGCCGCg
     2281 gaaacacagA AAAAAGCCCG CACCTGACAG TGCGGGCTTT TTTTTTcgac caaaggTAGC
     2341 GAACGACGAG TCACTGTTGA GGATAAATAC TTTCTCTACT AGGCGCCTGT TACACAGGTC
     2401 CTCAGCGGCG CGCCTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGGGAGCG
     2461 GATTTGAACG TTGTGAAGCA ACGGCCCGGA GGGTGGCGGG CAGGACGCCC GCCATAAACT
     2521 GCCAGGCATC AAACTAAGCA GAAGGCCATC CTGACGGATG GCCTTTTTGC GTTTCAGATC
     2581 TACCGGTaaa ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga
     2641 caagctgacg accgggtctc cgcaagtggc acttttcggg gaaatgtgcg cggaacccct
     2701 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa
     2761 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     2821 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     2881 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     2941 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     3001 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     3061 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     3121 agcgagacga aatacgcggt cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     3181 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     3241 taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg
     3301 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     3361 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     3421 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     3481 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga
     3541 gcaagacgtt tcccgttgaa tatggctcat actcttcctt tttcaatatt attgaagcat
     3601 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3661 aataggcatg ctagcgcaga aacgtcctag aagatgccag gaggatactt agcagagaga
     3721 caataaggcc ggagcgaagc cgtttttcca taggctccgc ccccctgacg aacatcacga
     3781 aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3841 tccccctgat ggctccctct tgcgctctcc tgttcccgtc ctgcggcgtc cgtgttgtgg
     3901 tggaggcttt acccaaatca ccacgtcccg ttccgtgtag acagttcgct ccaagctggg
     3961 ctgtgtgcaa gaaccccccg ttcagcccga ctgctgcgcc ttatccggta actatcatct
     4021 tgagtccaac ccggaaagac acgacaaaac gccactggca gcagccattg gtaactgaga
     4081 attagtggat ttagatatcg agagtcttga agtggtggcc taacagaggc tacactgaaa
     4141 ggacagtatt tggtatctgc gctccactaa agccagttac caggttaagc agttccccaa
     4201 ctgacttaac cttcgatcaa accgcctccc caggcggttt tttcgtttac agagcaggag
     4261 attacgacga tcgtaaaagg atctcaagaa gatcctttac ggattcccga caccatcact
     4321 ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata
     4381 taagttgtaa ttctcatgtt agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     4441 CTCCTAgGGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA TTCACTTTTT
     4501 CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA AATACCCGCG
     4561 AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA GGCATCCGGG
     4621 TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG CTTAAGACGC
     4681 TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG CAAACATGCT
     4741 GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG TACTGACAAG
     4801 CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT TCCATGCGCC
     4861 GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC CCTTCCCCTT
     4921 GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC GCTTCATCCG
     4981 GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA TGCCAGTAGG
     5041 CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA TGACGACCGT
     5101 AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA ACAAATTCTC
     5161 GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA TAACCTTTCA
     5221 TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC GGCGTTAAAC
     5281 CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT TGCGCTTCAG
     5341 CCAT
//