LOCUS       pEND-ZY                 5686 bp ds-DNA     circular     15-APR-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        274..302
                     /label="P(BAD) promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    315..320
                     /label="EcoRI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    321..350
                     /label="VL2017mar22_14"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    351..356
                     /label="SacI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    357..371
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    372..391
                     /label="Csy4 cut site - Tsai et al 2014"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    377..391
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    391..391
                     /label="Csy4 cuts after this G"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    392..411
                     /label="sgRNA-2 (orthogonal set from Didovyk et al 2015)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    392..497
                     /label="sgRNA-2 (orthogonal set from Didovyk et al 2015)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    412..497
                     /label="sgRNA(constant) optimised (Dang et al 2015) without terminator"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    416..416
                     /label="T->C change to optimise (Dang et al 2015)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    424..428
                     /label="extended hairpin (Dang et al 2015)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    429..432
                     /label="loop"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(433..437)
                     /label="extended hairpin (Dang et al 2015)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    442..444
                     /label="not annealing"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(447..447)
                     /label="T->C change to optimise (Dang et al 2015)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    498..517
                     /label="Csy4 cut site - Tsai et al 2014"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    503..517
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    517..517
                     /label="Csy4 cuts after this G"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    518..532
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    533..538
                     /label="XhoI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    539..568
                     /label="VL2017mar22_0"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    569..574
                     /label="NcoI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    575..664
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    665..864
                     /label="2017mar28_200bpSpacer1* (C->G change to remove AGCAGY)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    787..787
                     /label="C->G change to remove AGCAGY"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    865..870
                     /label="HindIII"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    871..885
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    886..920
                     /label="P(BBa_J23100)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    921..935
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    936..965
                     /label="VL2017mar22_24"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    966..980
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(981..983)
                     /label="PAM"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(981..1003)
                     /label="bssgRNA-1 _ ANTISENSE is identical (orthogonal set from Didovyk et al 2015)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(984..1003)
                     /label="orthogonal bssgRNA-1 _ ANTISENSE is identical (Didovyk et al 2015)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1004..1018
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1019..1048
                     /label="VL2017mar22_22"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1049..1063
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1064..1083
                     /label="Csy4 cut site - Tsai et al 2014"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1069..1083
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1083..1083
                     /label="Csy4 cuts after this G"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1084..1117
                     /label="RBS"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     misc_feature    1105..1110
                     /label="Shine-Dalgarno ?"
                     /ApEinfo_revcolor="#fdc68a"
                     /ApEinfo_fwdcolor="#fdc68a"
     gene            1118..1831
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1118..1897
                     /label="Translation 1118-1897"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKMSRRNTDAITIHSILDWIED**"
     misc_feature    1832..1897
                     /label="MarAn20 degradation tag (C-term)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1898..1912
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1913..1918
                     /label="BamHI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1919..1948
                     /label="Linker_10"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1949..1956
                     /label="SbfI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1957..2017
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2018..2217
                     /label="2018mar28_200bpSpacer2.5* (A->T change to remove AGCAGY)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2079..2079
                     /label="A->T change to remove AGCAGY"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2218..2223
                     /label="SalI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2224..2238
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2239..2273
                     /label="P(BBa_J23150) - weak"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2274..2288
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2289..2318
                     /label="VL2017mar22_19"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2319..2333
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(2334..2336)
                     /label="PAM"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(2334..2356)
                     /label="bssgRNA-2 _ ANTISENSE is identical (orthogonal set from Didovyk et al 2015)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(2337..2356)
                     /label="orthogonal bssgRNA-2 _ ANTISENSE is identical (Didovyk et al 2015)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2357..2371
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2372..2401
                     /label="VL2017mar22_17"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2402..2416
                     /label="Prefix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2417..2436
                     /label="Csy4 cut site - Tsai et al 2014"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2422..2436
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2436..2436
                     /label="Csy4 cuts after this G"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2437..2456
                     /label="orthogonal gRNA-1 (Didovyk et al 2015)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2437..2542
                     /label="sgRNA-1 (orthogonal set from Didovyk et al 2015)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2457..2542
                     /label="sgRNA(constant) optimised (Dang et al 2015) without terminator"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2461..2461
                     /label="T->C change to optimise (Dang et al 2015)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2469..2473
                     /label="extended hairpin (Dang et al 2015)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2474..2477
                     /label="loop"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(2478..2482)
                     /label="extended hairpin (Dang et al 2015)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2487..2489
                     /label="not annealing"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(2492..2492)
                     /label="T->C change to optimise (Dang et al 2015)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2543..2562
                     /label="Csy4 cut site - Tsai et al 2014"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2548..2562
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2562..2562
                     /label="Csy4 cuts after this G"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2563..2577
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2578..2583
                     /label="AatII"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2584..2613
                     /label="Linker_11"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2614..2621
                     /label="NotI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2622..2678
                     /label="ECK120033737 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2679..2723
                     /label="Linker 8"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2724..2729
                     /label="KasI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2730..2741
                     /label="Spacer 15"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2742..2748
                     /label="BbvCI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2749..2756
                     /label="AscI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            complement(3097..3912)
                     /label="KanR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     oriT            4022..4657
                     /label="ColA ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            complement(4808..5686)
                     /label="araC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 ACTTTTCATA CTCCCGCCAT TCAGAGAAGA AACCAATTGT CCATATTGCA TCAGACATTG
       61 CCGTCACTGC GTCTTTTACT GGCTCTTCTC GCTAACCAAA CCGGTAACCC CGCTTATTAA
      121 AAGCATTCTG TAACAAAGCG GGACCAAAGC CATGACAAAA ACGCGTAACA AAAGTGTCTA
      181 TAATCACGGC AGAAAAGTCC ACATTGATTA TTTGCACGGC GTCACACTTT GCTATGCCAT
      241 AGCATTTTTA TCCATAAGAT TAGCGGtTCC TACCTGACGC TTTTTATCGC AACTCTCTAC
      301 TGTTTCTCCA TACCGAATTC ATAGGATAGA TTCTGGAAAC TTTACCGTCC GAGCTCCAGC
      361 CTGCGGTCCG GGTTCACTGC CGTATAGGCA GATCAGTGTG TACTAAGTAC TGTTTcAGAG
      421 CTATGCTGGA AACAGCATAG CAAGTTgAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG
      481 TGGCACCGAG TCGGTGCGTT CACTGCCGTA TAGGCAGTCG CTGGGACGCC CGCTCGAGCA
      541 ATAAACAGTT GATAGGGCTT CTCCGTTACC ATGGTTCAGC CAAAAAACTT AAGACCGCCG
      601 GTCTTGTCCA CTACCTTGCA GTAATGCGGT GGACAGGATC GGCGGTTTTC TTTTCTCTTC
      661 TCAATTCTTC TGACCTGTAA CGAATAATAG ATAGTAAAGT AGTCTCCGAT TGAGTTTTCT
      721 CTGCCGAGTC CCACCCAGTT CTGTGATTTC AGTAAGTTGG TAATTGATAC ACTGTTGCGA
      781 GAACTGgTGC CTGGTAGTAG ATAGGTTGTT ATTGAGTAAG AAGGTAAAGT GAACGAAATC
      841 CCTGAAACTG AGACTGTAGA AAATAAGCTT CAGCCTGCGG TCCGGttgac ggctagctca
      901 gtcctaggta cagtgctagc TCGCTGGGAC GCCCGAGATA GCCGTTACAC AGGTGACACT
      961 TATTTCAGCC TGCGGTCCGG ccaCACTAGA GTCTAGCTTG AGATCGCTGG GACGCCCGTA
     1021 GAACTCTGTG AGGATAAAGT CTCCCTTACA GCCTGCGGTC CGGGTTCACT GCCGTATAGG
     1081 CAGTAATTTT GTTTAACTTT AAGAAGGAGA TATACATATG CGTAAAGGCG AAGAACTGTT
     1141 TACCGGTGTG GTTCCGATTC TGGTGGAACT GGACGGCGAT GTTAATGGTC ATAAATTCAG
     1201 TGTTCGCGGC GAAGGTGAAG GCGATGCGAC GAACGGCAAA CTGACCCTGA AATTTATCTG
     1261 CACCACGGGT AAACTGCCGG TCCCGTGGCC GACGCTGGTG ACCACGCTGA CCTATGGCGT
     1321 TCAATGTTTT GCGCGTTACC CGGATCACAT GAAACAGCAC GACTTTTTCA AATCGGCCAT
     1381 GCCGGAAGGC TATGTGCAGG AACGTACGAT TAGCTTTAAA GACGATGGTA CGTATAAAAC
     1441 CCGCGCGGAA GTGAAATTCG AAGGCGATAC CCTGGTTAAC CGTATCGAAC TGAAAGGTAT
     1501 CGATTTCAAA GAAGACGGCA ATATTCTGGG TCATAAACTG GAATATAACT TCAATTCCCA
     1561 CAACGTGTAC ATCACCGCGG ATAAACAGAA AAACGGCATT AAAGCCAATT TCAAAATCCG
     1621 CCATAATGTG GAAGATGGTA GCGTTCAGCT GGCCGACCAC TATCAGCAAA ACACGCCGAT
     1681 TGGTGATGGC CCGGTCCTGC TGCCGGACAA TCACTACCTG AGTACCCAGT CCGTGCTGTC
     1741 AAAAGATCCG AACGAAAAAC GTGACCACAT GGTCCTGCTG GAATTTGTGA CGGCTGCGGG
     1801 TATCACCCAC GGCATGGACG AACTGTATAA AATGTCCCGC CGTAATACTG ACGCCATCAC
     1861 AATCCACAGC ATCCTGGATT GGATTGAAGA CTAATAATCG CTGGGACGCC CGGGATCCAA
     1921 GAGATTTCTA CACGATTGAG CACTGTCTCC TGCAGGCTCG GTACCAAATT CCAGAAAAGA
     1981 GGCCTCCCGA AAGGGGGGCC TTTTTTCGTT TTGGTCCTAA TAGATAAAGG ATAGGTCTGG
     2041 TAGTGTTGTT CGTTCTCGCA GGTAAATCAA TAATACTCtG CAGTTCCGTA GACTTTTCAG
     2101 TGGGACAGGG TAGCGATAAC AGATAGATTG TAATAAGACA CAGTAGGTGC TCGTAGTTGC
     2161 GTGAAGAGAA CCGCTCAGGA AATCCAGTCA GAAGTATTGG TAATCGTTGA AAACTCAGTC
     2221 GACCAGCCTG CGGTCCGGTT TACGGCTAGC TCAGTCCTAG GTATTATGCT AGCTCGCTGG
     2281 GACGCCCGTG AACAGTTGCT CTGATTGAAA CCACGATTCA GCCTGCGGTC CGGccaAGTA
     2341 CTTAGTACAC ACTGATTCGC TGGGACGCCC GGTAGTGCTT ATCAGACCCA ATACTGTTGA
     2401 ACAGCCTGCG GTCCGGGTTC ACTGCCGTAT AGGCAGTCTC AAGCTAGACT CTAGTGGTTT
     2461 cAGAGCTATG CTGGAAACAG CATAGCAAGT TgAAATAAGG CTAGTCCGTT ATCAACTTGA
     2521 AAAAGTGGCA CCGAGTCGGT GCGTTCACTG CCGTATAGGC AGTCGCTGGG ACGCCCGGAC
     2581 GTCCTATTAC ACTCGTCGTT GGAAACTGAA GATGCGGCCG Cggaaacaca gAAAAAAGCC
     2641 CGCACCTGAC AGTGCGGGCT TTTTTTTTcg accaaaggTA GCGAACGACG AGTCACTGTT
     2701 GAGGATAAAT ACTTTCTCTA CTAGGCGCCT GTTACACAGG TCCTCAGCGG CGCGCCTTTG
     2761 TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGTGAAG
     2821 CAACGGCCCG GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAACTAAG
     2881 CAGAAGGCCA TCCTGACGGA TGGCCTTTTT GCGTTTCAGA TCTACCGGTa aaccagcaat
     2941 agacataagc ggctatttaa cgaccctgcc ctgaaccgac gacaagctga cgaccgggtc
     3001 tccgcaagtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3061 atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa
     3121 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     3181 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     3241 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     3301 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     3361 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     3421 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     3481 gtcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     3541 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     3601 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     3661 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     3721 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     3781 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     3841 atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg
     3901 aatatggctc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3961 catgagcgga tacatatttg aatgtattta gaaaaataaa caaataggca tgctagcgca
     4021 gaaacgtcct agaagatgcc aggaggatac ttagcagaga gacaataagg ccggagcgaa
     4081 gccgtttttc cataggctcc gcccccctga cgaacatcac gaaatctgac gctcaaatca
     4141 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg atggctccct
     4201 cttgcgctct cctgttcccg tcctgcggcg tccgtgttgt ggtggaggct ttacccaaat
     4261 caccacgtcc cgttccgtgt agacagttcg ctccaagctg ggctgtgtgc aagaaccccc
     4321 cgttcagccc gactgctgcg ccttatccgg taactatcat cttgagtcca acccggaaag
     4381 acacgacaaa acgccactgg cagcagccat tggtaactga gaattagtgg atttagatat
     4441 cgagagtctt gaagtggtgg cctaacagag gctacactga aaggacagta tttggtatct
     4501 gcgctccact aaagccagtt accaggttaa gcagttcccc aactgactta accttcgatc
     4561 aaaccgcctc cccaggcggt tttttcgttt acagagcagg agattacgac gatcgtaaaa
     4621 ggatctcaag aagatccttt acggattccc gacaccatca ctctagattt cagtgcaatt
     4681 tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg
     4741 ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgCTCCTAgG GTCTGATTCG
     4801 TTACCAATTA TGACAACTTG ACGGCTACAT CATTCACTTT TTCTTCACAA CCGGCACGGA
     4861 ACTCGCTCGG GCTGGCCCCG GTGCATTTTT TAAATACCCG CGAGAAATAG AGTTGATCGT
     4921 CAAAACCAAC ATTGCGACCG ACGGTGGCGA TAGGCATCCG GGTGGTGCTC AAAAGCAGCT
     4981 TCGCCTGGCT GATACGTTGG TCCTCGCGCC AGCTTAAGAC GCTAATCCCT AACTGCTGGC
     5041 GGAAAAGATG TGACAGACGC GACGGCGACA AGCAAACATG CTGTGCGACG CTGGCGATAT
     5101 CAAAATTGCT GTCTGCCAGG TGATCGCTGA TGTACTGACA AGCCTCGCGT ACCCGATTAT
     5161 CCATCGGTGG ATGGAGCGAC TCGTTAATCG CTTCCATGCG CCGCAGTAAC AATTGCTCAA
     5221 GCAGATTTAT CGCCAGCAGC TCCGAATAGC GCCCTTCCCC TTGCCCGGCG TTAATGATTT
     5281 GCCCAAACAG GTCGCTGAAA TGCGGCTGGT GCGCTTCATC CGGGCGAAAG AACCCCGTAT
     5341 TGGCAAATAT TGACGGCCAG TTAAGCCATT CATGCCAGTA GGCGCGCGGA CGAAAGTAAA
     5401 CCCACTGGTG ATACCATTCG CGAGCCTCCG GATGACGACC GTAGTGATGA ATCTCTCCTG
     5461 GCGGGAACAG CAAAATATCA CCCGGTCGGC AAACAAATTC TCGTCCCTGA TTTTTCACCA
     5521 CCCCCTGACC GCGAATGGTG AGATTGAGAA TATAACCTTT CATTCCCAGC GGTCGGTCGA
     5581 TAAAAAAATC GAGATAACCG TTGGCCTCAA TCGGCGTTAA ACCCGCCACC AGATGGGCAT
     5641 TAAACGAGTA TCCCGGCAGC AGGGGATCAT TTTGCGCTTC AGCCAT
//