LOCUS       Exported               10011 bp ds-DNA     circular SYN 18-APR-2025
DEFINITION  synthetic linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 18, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10011
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(225..246)
                     /label=SMP1803
     promoter        385..662
                     /label=chicken beta-actin promoter
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1622
                     /label=SMP3903
     primer_bind     1633..1650
                     /label=SMP2546
     primer_bind     1640..1659
                     /label=SMP2581
     primer_bind     1640..1656
                     /label=SMP2817
     primer_bind     1644..1659
                     /label=SMP4427
     primer_bind     1681..1698
                     /label=SMP2571
     primer_bind     1720..1734
                     /label=SMP2574
     regulatory      1753..1762
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
     CDS             1768..2868
                     /codon_start=1
                     /label=MBP tag
                     /note="MBP tag"
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     CDS             1768..2868
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1851..1865
                     /label=SMP2573
     primer_bind     2221..2240
                     /label=SMP2591
     misc_feature    2445..2460
                     /label=SMP2548
     primer_bind     2445..2460
                     /label=SMP2548
     primer_bind     complement(2567..2585)
                     /label=SMP3658
     primer_bind     2827..2845
                     /label=malE
     CDS             2887..2910
                     /codon_start=1
                     /label=Strep tag
                     /note="Strep tag"
                     /translation="WSHPQFEK"
     CDS             2887..2910
                     /codon_start=1
                     /label=Strep tag
                     /translation="WSHPQFEK"
     CDS             2947..2970
                     /codon_start=1
                     /label=Strep tag
                     /note="Strep tag"
                     /translation="WSHPQFEK"
     CDS             2947..2970
                     /codon_start=1
                     /label=Strep tag
                     /translation="WSHPQFEK"
     CDS             2977..3000
                     /codon_start=1
                     /label=Flag tag
                     /note="Flag tag"
                     /translation="DYKDDDDK"
     CDS             3007..3027
                     /codon_start=1
                     /label=TEV cleavage site
                     /note="TEV cleavage site"
                     /translation="ENLYFQS"
     primer_bind     3009..3051
                     /label=SMC4840
     misc_feature    3011..3054
                     /label=AgeI-GFP fw
     misc_feature    3028..3033
                     /label=AgeI (2x)
     primer_bind     3029..3053
                     /label=GFP fwd
     CDS             3034..3750
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     3034..3053
                     /label=SMP_2235
     primer_bind     3034..3053
                     /label=SMP_3364
     primer_bind     3037..3054
                     /label=SMP_2824
     primer_bind     complement(3037..3054)
                     /label=SMP_3729
     primer_bind     3040..3057
                     /label=SMP_2016
     primer_bind     complement(3076..3092)
                     /label=PEN1-737R
     primer_bind     complement(3079..3100)
                     /label=SMP_3301
     primer_bind     complement(3081..3101)
                     /label=SMP_919
     primer_bind     complement(3096..3114)
                     /label=SMP_2564
     primer_bind     complement(3107..3127)
                     /label=SMP_2562
     primer_bind     complement(3109..3127)
                     /label=EGFP-N-R+33
     primer_bind     complement(3142..3160)
                     /label=EGFP-N-rev
     primer_bind     complement(3150..3169)
                     /label=SMP_2433
     primer_bind     complement(3165..3183)
                     /label=SMP_3388
     primer_bind     3208..3228
                     /label=SMP_2316
     primer_bind     3282..3301
                     /label=SMP_1001
     primer_bind     3328..3347
                     /label=SMP_1325
     primer_bind     complement(3338..3358)
                     /label=SMP_2969
     primer_bind     complement(3395..3415)
                     /label=SMP_2971
     primer_bind     complement(3398..3414)
                     /label=SMP_2578
     primer_bind     complement(3428..3441)
                     /label=SMP_2969
     primer_bind     3441..3459
                     /label=SMP_444
     primer_bind     3451..3475
                     /label=SMP_1666
     primer_bind     3505..3522
                     /label=SMP_3040
     primer_bind     3505..3522
                     /label=SMP_3104
     primer_bind     3510..3529
                     /label=SMP_1330
     primer_bind     3536..3553
                     /label=SMP_4080
     primer_bind     3537..3556
                     /label=SMP_437
     primer_bind     complement(3537..3556)
                     /label=SMP_471
     primer_bind     3575..3592
                     /label=SMP_3892
     primer_bind     3588..3607
                     /label=SMP_364
     primer_bind     3637..3669
                     /label=SMP_295
     primer_bind     complement(3637..3669)
                     /label=SMP_296
     primer_bind     3660..3678
                     /label=EGFP-C-F-31
     primer_bind     3669..3685
                     /label=EGFP-for
     primer_bind     3675..3693
                     /label=SMP_564
     primer_bind     3687..3708
                     /label=SMP_3300
     primer_bind     3691..3708
                     /label=EGFP-C-for
     primer_bind     complement(3723..3741)
                     /label=SMP2415
     misc_feature    3729..3772
                     /label=AgeI-GFP rev
     primer_bind     complement(3730..3750)
                     /label=SMP2429
     primer_bind     complement(3730..3749)
                     /label=SMP2596
     primer_bind     complement(3731..3775)
                     /label=SMC4841
     primer_bind     3751..3769
                     /label=GFP fwd
     misc_feature    3751..3756
                     /label=AgeI (2x)
     CDS             3766..6915
                     /codon_start=1
                     /label=hULK1
                     /translation="MEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAV
                     KCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYL
                     HAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKI
                     ADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQ
                     ASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDAS
                     PSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHS
                     SRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHG
                     RTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPR
                     SSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSPQVGTIPERPGW
                     SGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTD
                     PLGAVFSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPK
                     TPSSQNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRSFS
                     TSRLTDLLLKAAFGTQAPDPGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSPVV
                     FTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPELPAPGHGCSFA
                     DPITANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQHVLEIAALKGSASEAA
                     GGPEYQLQESVVADQISLLSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSS
                     TVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQ
                     SAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGI
                     CA"
     primer_bind     complement(3883..3900)
                     /label=SMP3452
     misc_feature    3958..3973
                     /label=SMP2549
     primer_bind     3958..3973
                     /label=SMP2549
     primer_bind     4450..4469
                     /label=SMP2580
     misc_feature    4806..4821
                     /label=SMP2550
     primer_bind     4806..4821
                     /label=SMP2550
     misc_feature    5627..5644
                     /label=SMP2551
     primer_bind     5627..5644
                     /label=SMP2551
     misc_feature    6435..6450
                     /label=SMP2552
     primer_bind     6435..6450
                     /label=SMP2552
     primer_bind     6925..6955
                     /label=SMP3881
     primer_bind     complement(6985..7000)
                     /label=SMP2553
     primer_bind     complement(7081..7096)
                     /label=SMP2818
     polyA_signal    7098..7153
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7468..7507)
                     /label=SMP3862
     misc_feature    7474..7517
                     /label=HindIII rev
     primer_bind     complement(7502..7523)
                     /label=SMP3902
     primer_bind     complement(7514..7530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7514..7530)
                     /label=SMP1770
     primer_bind     complement(7516..7535)
                     /label=SMP993
     primer_bind     complement(7527..7547)
                     /label=SMP547
     primer_bind     complement(7528..7547)
                     /label=LacOp-for
     protein_bind    7538..7554
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7562..7592)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(7562..7581)
                     /label=pUCM13-rev-157
     protein_bind    7607..7628
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     7641..7658
                     /label=SMP4078
     primer_bind     complement(7643..7660)
                     /label=SMP2212
     promoter        7687..7882
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     primer_bind     7717..7736
                     /label=SV40-for
     rep_origin      7733..7868
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7738..7754
                     /label=SV40-for
     polyA_signal    7888..8022
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7888..7905)
                     /label=EGFP-C-Rev
     primer_bind     7925..7946
                     /label=pCI-rev
     primer_bind     complement(7929..7948)
                     /label=SMP3314
     primer_bind     complement(7966..7987)
                     /label=SMP3893
     primer_bind     7979..7998
                     /label=EBV-rev
     rep_origin      complement(8261..8849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9020..9880)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9070..9089)
                     /label=AmpStop
     primer_bind     9636..9653
                     /label=SMP4076
     primer_bind     9816..9835
                     /label=AmpStart
     promoter        complement(9881..9985)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9966..9986)
                     /label=SMP646
     primer_bind     9966..9986
                     /label=SMP654
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcctcgatc
     1741 gacggtatcg atgccaccat ggctagcatg aaaatcgaag aaggtaaact ggtaatctgg
     1801 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1861 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1921 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1981 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     2041 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     2101 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     2161 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     2221 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2281 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2341 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2401 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2461 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2521 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2581 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2641 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2701 ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     2761 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2821 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2881 gctagctgga gccacccgca gttcgagaaa ggtggaggtg cccgaggtgg atcgggaggt
     2941 ggatcgtgga gccacccgca gttcgaaaaa ggattcgatt acaaggatga cgatgacaag
     3001 ggtaccgaaa acctgtactt ccaatccacc ggtatggtga gcaagggcga ggagctgttc
     3061 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     3121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     3181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     3241 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     3301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     3361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     3421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     3481 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     3541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     3601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
     3661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     3721 atcactctcg gcatggacga gctgtacaag accggtggtc ccgggatgga acctggcaga
     3781 ggaggaacag aaaccgtcgg aaaattcgag tttagcagaa aggacctgat tggacatgga
     3841 gcattcgcag tcgtgttcaa gggtcgtcac cgggaaaaac atgacctgga ggtggccgtc
     3901 aagtgcatca acaagaaaaa tctggctaaa tcccagaccc tgctcggcaa ggaaatcaaa
     3961 attctgaagg agctcaaaca cgaaaacatt gtggccctgt acgacttcca ggaaatggct
     4021 aactctgtgt acctggtcat ggagtattgc aatggcggag acctggctga ttatctccat
     4081 gcaatgagaa ccctgagcga ggacactatc cgtctgttcc tccagcagat tgctggtgca
     4141 atgcgtctgc tccactccaa gggcatcatt catcgggatc tgaaacccca gaacatcctg
     4201 ctctccaatc ctgctggccg cagggcaaac ccaaattcta tcagggtgaa gattgccgac
     4261 ttcggatttg ctagatacct gcagtctaac atgatggccg ctacactgtg cggttcacct
     4321 atgtacatgg ctccagaggt cattatgtca cagcactatg acggaaaggc cgatctgtgg
     4381 agtatcggta ctattgtgta ccagtgtctg acaggcaaag cccctttcca ggctagctcc
     4441 ccacaggacc tgcgcctctt ttatgaaaag aacaaaaccc tggtgccaac tatcccccgt
     4501 gagacctctg cacctctgcg tcagctgctc ctggcactcc tgcagagaaa tcacaaggac
     4561 cgtatggact tcgatgagtt ctttcaccat ccctttctgg atgcttcacc tagtgtgagg
     4621 aaatcccccc ctgtgcctgt cccaagctac ccatcttcag gttccggcag tagctcctct
     4681 tcaagtagca cctctcacct ggcatcccca ccatctctcg gagagatgca gcagctgcag
     4741 aagaccctcg cctcacccgc tgacactgca ggattcctgc attcctctag agattcaggt
     4801 ggcagtaaag actcaagttg cgatactgac gatttcgtga tggtccccgc tcagtttcct
     4861 ggagacctgg tcgccgaagc tcctagcgcc aagcctccac ccgattccct gatgtgttct
     4921 ggtagctccc tggtggcatc tgcaggtctc gagtcacacg gtcgtacccc aagcccttcc
     4981 cctccatgct cttcaagtcc atctccctca ggacgggcag gtccattcag ctcctctcgc
     5041 tgtggcgcct ctgtgcctat cccagtcccc acacaggtgc agaactacca gcggattgaa
     5101 cgcaatctgc agagcccaac acagttccag accccccgtt caagtgctat cagacgtagt
     5161 ggcagcacct ccccactggg atttgctcgt gcatctccat caccacctgc acacgctgag
     5221 catggaggtg tcctggccag gaagatgagt ctcggcggag gtagacccta tacacctagc
     5281 ccacaagtgg gtaccattcc tgaaaggcca ggctggtcag gaacccccag tcctcagggt
     5341 gctgagatga ggggcggaag atccccacgt ccaggtagct ccgcaccaga gcacagccca
     5401 cgtacttccg gactgggttg caggctccac agtgctccta acctgagcga cctccatgtg
     5461 gtccgtccca agctgcctaa accacccacc gatcccctgg gagcagtgtt ctcccctcca
     5521 caggcatctc cacctcagcc ttcacatgga ctgcagagtt gtcggaacct ccgcggtagc
     5581 ccaaagctgc ccgacttcct ccagagaaat cccctgccac ccatcctcgg atcccccaca
     5641 aaggccgtcc cttctttcga ttttcctaaa accccatctt cacagaacct cctggccctc
     5701 ctggctcgtc agggtgtggt catgacacct ccacgcaata ggaccctgcc agacctctcc
     5761 gaagtgggtc ctttccacgg acagccactg ggtcctggac tccgtccagg cgaggatcca
     5821 aagggtccct tcggccggag ttttagcact tcccgcctga cagacctcct gctcaaagca
     5881 gccttcggta cacaggcccc tgatccaggc agcaccgagt ctctgcagga gaagccaatg
     5941 gagatcgcac cctctgccgg ctttggtggt tcactgcacc caggagcaag ggcaggaggt
     6001 accagtagcc cctcacctgt ggtcttcact gtgggaagtc ccccttctgg ttcaactcca
     6061 ccccagggcc ctagaacacg tatgtttagt gctggaccaa ctggtagcgc atcctcttca
     6121 gccagacacc tggtcccagg accctgcagc gaagctcctg caccagagct gccagcacct
     6181 ggtcatggtt gttccttcgc agaccctatc actgccaacc tggaaggcgc tgtgacattt
     6241 gaggcacctg atctgccaga ggaaaccctc atggagcagg aacacactga gatcctgagg
     6301 ggtctcagat tcaccctgct ctttgtgcag catgtcctgg aaattgctgc actcaagggt
     6361 agtgctagcg aggcagctgg tggaccagaa taccagctgc aggagagcgt ggtcgccgac
     6421 cagatctctc tgctctcaag ggaatggggc ttcgctgagc agctggtcct ctatctgaag
     6481 gtggccgaac tgctcagtag cggcctgcag tccgccatcg atcagattag agctggaaag
     6541 ctctgcctgt cctctactgt gaaacaggtg gtccggcgcc tgaacgagct ctacaaggcc
     6601 agtgtggtca gctgtcaggg actctctctg cgtctccagc ggttctttct ggacaaacag
     6661 cgcctgctcg ataggatcca ctcaattaca gctgaacgcc tgatcttcag ccatgcagtg
     6721 cagatggtcc agtccgcagc cctggacgaa atgtttcagc acagggaggg atgcgtcccc
     6781 agataccata aggctctgct cctgctcgag ggtctgcagc acatgctcag cgaccaggcc
     6841 gatatcgaaa atgtgactaa gtgcaaactg tgtattgaga ggagactctc cgctctgctc
     6901 acaggcatct gtgcataacc cgggtgatat cttaattaat taactcgagg aattcactcc
     6961 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     7021 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     7081 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     7141 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     7201 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     7261 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     7321 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     7381 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     7441 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
     7501 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7561 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7621 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7681 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7741 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7801 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7861 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
     7921 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7981 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg
     8041 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8101 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8161 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8221 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8281 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8341 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8401 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     8461 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8521 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8581 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8641 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8701 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8761 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8881 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8941 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9001 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9061 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9121 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9181 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9241 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9301 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9361 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9421 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9481 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9541 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9601 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9661 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9721 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9781 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9841 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9901 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9961 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
