LOCUS       VB200922-1491fbs        6096 bp    DNA     circular SYN 21-APR-2025
DEFINITION  
ACCESSION   VB200922-1491fbs
VERSION     VB200922-1491fbs
KEYWORDS    .
SOURCE      
  ORGANISM  .
            
FEATURES             Location/Qualifiers
     source          1..6096
     ITR             1..141
                     /application_notes="Allows rescue of virus from recombinant
                     plasmid and replication of the viral genome; this ITR is
                     identical to that of the wild-type AAV2 genome."
                     /description="AAV 5' inverted terminal repeat (functional
                     equivalent of wild-type 5' ITR)"
                     /label="5' ITR"
                     /note="color: #05696d; direction: RIGHT"
                     /official_designation="5' ITR"
                     /uuid="5f14ec1a645a407dab2725d71bd1c86a"
     Promoter        169..1901
                     /application_notes="Strong promoter."
                     /description="CMV early enhancer fused to modified chicken
                     beta-actin promoter"
                     /full_name="Cytomegalovirus early enhancer fused with
                     chicken beta-actin promoter"
                     /label="CAG"
                     /note="color: #f54c6b; direction: RIGHT"
                     /official_designation="CAG"
                     /uuid="420ea94873db443a902370384d17a441"
     Miscellaneous   1926..1959
                     /application_notes="Recognition site of Cre recombinase;
                     can't work with other types of Lox site."
                     /description="Mutated Lox site with two base substitutions
                     of LoxP"
                     /label="Lox2272"
                     /note="color: #944603; direction: RIGHT"
                     /official_designation="Lox2272"
                     /uuid="a19dd1e203df40eb846e5102df13ffb4"
     Miscellaneous   1992..2025
                     /application_notes="Recognition site of Cre recombinase."
                     /description="Locus of X(cross)-over in P1"
                     /label="LoxP"
                     /note="color: #ce0084; direction: RIGHT"
                     /official_designation="LoxP"
                     /uuid="b48e25a55648467f8c98b3f68faf613f"
     CDS             complement(2038..2373)
                     /application_notes=""
                     /label="{Beta-Endorphin}"
                     /note="color: #8ccc27; direction: LEFT"
                     /official_designation="{Beta-Endorphin}"
                     /uuid="6697e3ddc3164cf5b8e5e8c3f72fab10"
     Miscellaneous   complement(2374..2379)
                     /application_notes="Facilitates translation initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /description="Kozak translation initiation sequence"
                     /full_name="Kozak"
                     /label="Kozak"
                     /note="color: #6d3ed8; direction: LEFT"
                     /official_designation="Kozak"
                     /uuid="ba4f837cfff042e38e7561244b24cfa6"
     Miscellaneous   complement(2386..2419)
                     /application_notes="Recognition site of Cre recombinase;
                     can't work with other types of Lox site."
                     /description="Mutated Lox site with two base substitutions
                     of LoxP"
                     /label="Lox2272"
                     /note="color: #0375bb; direction: LEFT"
                     /official_designation="Lox2272"
                     /uuid="991e0551b2034abd9c3ad4e364260ba6"
     Miscellaneous   complement(2452..2485)
                     /application_notes="Recognition site of Cre recombinase."
                     /description="Locus of X(cross)-over in P1"
                     /label="LoxP"
                     /note="color: #a949ca; direction: LEFT"
                     /official_designation="LoxP"
                     /uuid="f4dc79ba31354e87bba6736ab88dbb23"
     Miscellaneous   2516..3113
                     /application_notes="Enhances virus stability in packaging
                     cells, leading to higher titer of packaged virus; enhances
                     higher expression of transgenes."
                     /description="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /full_name="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /label="WPRE"
                     /note="color: #946a00; direction: RIGHT"
                     /official_designation="WPRE"
                     /uuid="0aedff8308474b369c622433538d7836"
     PolyA_signal    3144..3351
                     /application_notes="Allows transcription termination and
                     polyadenylation of mRNA transcribed by Pol II RNA
                     polymerase."
                     /description="Bovine growth hormone polyadenylation signal"
                     /full_name="Bovine growth hormone polyadenylation"
                     /label="BGH pA"
                     /note="color: #512bbd; direction: RIGHT"
                     /official_designation="BGH pA"
                     /uuid="4a09734ca6c745b3aadc757c1638b651"
     ITR             complement(3359..3499)
                     /application_notes="Allows rescue of virus from recombinant
                     plasmid and replication of the viral genome; this ITR is
                     identical to that of the wild-type AAV2 genome."
                     /description="AAV 3' inverted terminal repeat"
                     /label="3' ITR"
                     /note="color: #d84e4e; direction: LEFT"
                     /official_designation="3' ITR"
                     /uuid="5b8101593b634e64aa9eef9434216f71"
     CDS             4416..5276
                     /application_notes="Allows E. coli to be resistant to
                     ampiciIIin."
                     /description="AmpiciIIin resistance gene"
                     /full_name="Ampicillin resistance gene"
                     /label="AmpiciIIin"
                     /note="color: #1f36a9; direction: RIGHT"
                     /official_designation="Ampicillin"
                     /uuid="5bcc09480c0e4c07b95ddccd8242d769"
     Rep_origin      5447..6035
                     /application_notes="Facilitates plasmid replication in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /description="pUC origin of replication"
                     /full_name="pUC origin of replication"
                     /label="pUC ori"
                     /note="color: #db6901; direction: RIGHT"
                     /official_designation="pUC ori"
                     /uuid="b978ad4ff785460db448caf63b728237"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttctagacaa ctttgtatag aaaagttgct cgacattgat
      181 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      241 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      301 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      361 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      421 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      481 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      541 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
      601 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
      661 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
      721 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
      781 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg
      841 cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
      901 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
      961 attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg
     1021 ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt
     1081 gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg
     1141 gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc
     1201 cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
     1261 ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc
     1321 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     1381 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     1441 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     1501 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     1561 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     1621 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     1681 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
     1741 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     1801 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     1861 tgctggttat tgtgctgtct catcattttg gcaaagaatt gcaagtttgt acaaaaaagc
     1921 aggctataac ttcgtatagg atactttata cgaagttatc ctcgagtacc ggtcacgcgt
     1981 acgcaagctt gataacttcg tatagcatac attatacgaa gttatcctag ggccacctca
     2041 ctggcccttc ttgtgcgcgt tcttgatgat ggcgttcttg aagagcgtca ccaggggcgt
     2101 ctggctcttc tcggaggtca tgaagccacc gtaacgcttg tccttgggcg ggttgttttc
     2161 agtcaggggc tgttcatctc cgttgccagg aaacacgggc gtctccagcg agaggtcgag
     2221 tttgcaagcc cggatgcaag ccagcaggtt gctctccgtg gtgaggtcct ggcactggct
     2281 gctctccagg caccagctcc acacatctat ggaggtctga agcaggaggg ccagcaacag
     2341 ggcccctgag cgactgtagc agaatctcgg catggtggct ctagaataac ttcgtataaa
     2401 gtatcctata cgaagttata ggtacctgag ctcaacatcg atagcactag tataacttcg
     2461 tataatgtat gctatacgaa gttataccca gctttcttgt acaaagtggg aattccgata
     2521 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2581 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2641 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2701 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2761 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2821 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2881 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     2941 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3001 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3061 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgggaattcc
     3121 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3181 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3241 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     3301 gcaggacagc aagggggagg attgggaaga gaatagcagg catgctgggg agggccgcag
     3361 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     3421 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     3481 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     3541 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     3601 cggcgggggt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
     3661 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3721 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3781 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3841 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3901 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
     3961 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     4021 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     4081 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4141 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     4201 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     4261 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4321 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4381 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4441 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4501 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4561 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4621 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4681 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4741 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4801 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4861 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4921 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4981 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5041 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5101 ttgctgataa atctggagcc ggtgagcgtg gaagccgcgg tatcattgca gcactggggc
     5161 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5221 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5281 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5341 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5401 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5461 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5521 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5581 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5641 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5701 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5761 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5821 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5881 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5941 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6001 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6061 ggttcctggc cttttgctgg ccttttgctc acatgt
//
