LOCUS       VB200922-1492hxt        6174 bp    DNA     circular SYN 21-APR-2025
DEFINITION  
ACCESSION   VB200922-1492hxt
VERSION     VB200922-1492hxt
KEYWORDS    .
SOURCE      
  ORGANISM  .
            
FEATURES             Location/Qualifiers
     source          1..6174
     ITR             1..141
                     /application_notes="Allows rescue of virus from recombinant
                     plasmid and replication of the viral genome; this ITR is
                     identical to that of the wild-type AAV2 genome."
                     /description="AAV 5' inverted terminal repeat (functional
                     equivalent of wild-type 5' ITR)"
                     /label="5' ITR"
                     /note="color: #e4b930; direction: RIGHT"
                     /official_designation="5' ITR"
                     /uuid="5f14ec1a645a407dab2725d71bd1c86a"
     Promoter        169..1901
                     /application_notes="Strong promoter."
                     /description="CMV early enhancer fused to modified chicken
                     beta-actin promoter"
                     /full_name="Cytomegalovirus early enhancer fused with
                     chicken beta-actin promoter"
                     /label="CAG"
                     /note="color: #7a00b5; direction: RIGHT"
                     /official_designation="CAG"
                     /uuid="420ea94873db443a902370384d17a441"
     Miscellaneous   1926..1959
                     /application_notes="Recognition site of Cre recombinase;
                     can't work with other types of Lox site."
                     /description="Mutated Lox site with two base substitutions
                     of LoxP"
                     /label="Lox2272"
                     /note="color: #05696d; direction: RIGHT"
                     /official_designation="Lox2272"
                     /uuid="a19dd1e203df40eb846e5102df13ffb4"
     Miscellaneous   1992..2025
                     /application_notes="Recognition site of Cre recombinase."
                     /description="Locus of X(cross)-over in P1"
                     /label="LoxP"
                     /note="color: #f54c6b; direction: RIGHT"
                     /official_designation="LoxP"
                     /uuid="b48e25a55648467f8c98b3f68faf613f"
     CDS             complement(2038..2451)
                     /application_notes=""
                     /label="{Alpha-MSH}"
                     /note="color: #0c9894; direction: LEFT"
                     /official_designation="{Alpha-MSH}"
                     /uuid="9b708c76f28a48f19a8e43cea6d98343"
     Miscellaneous   complement(2452..2457)
                     /application_notes="Facilitates translation initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /description="Kozak translation initiation sequence"
                     /full_name="Kozak"
                     /label="Kozak"
                     /note="color: #944603; direction: LEFT"
                     /official_designation="Kozak"
                     /uuid="ba4f837cfff042e38e7561244b24cfa6"
     Miscellaneous   complement(2464..2497)
                     /application_notes="Recognition site of Cre recombinase;
                     can't work with other types of Lox site."
                     /description="Mutated Lox site with two base substitutions
                     of LoxP"
                     /label="Lox2272"
                     /note="color: #ce0084; direction: LEFT"
                     /official_designation="Lox2272"
                     /uuid="991e0551b2034abd9c3ad4e364260ba6"
     Miscellaneous   complement(2530..2563)
                     /application_notes="Recognition site of Cre recombinase."
                     /description="Locus of X(cross)-over in P1"
                     /label="LoxP"
                     /note="color: #6d3ed8; direction: LEFT"
                     /official_designation="LoxP"
                     /uuid="f4dc79ba31354e87bba6736ab88dbb23"
     Miscellaneous   2594..3191
                     /application_notes="Enhances virus stability in packaging
                     cells, leading to higher titer of packaged virus; enhances
                     higher expression of transgenes."
                     /description="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /full_name="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /label="WPRE"
                     /note="color: #0375bb; direction: RIGHT"
                     /official_designation="WPRE"
                     /uuid="0aedff8308474b369c622433538d7836"
     PolyA_signal    3222..3429
                     /application_notes="Allows transcription termination and
                     polyadenylation of mRNA transcribed by Pol II RNA
                     polymerase."
                     /description="Bovine growth hormone polyadenylation signal"
                     /full_name="Bovine growth hormone polyadenylation"
                     /label="BGH pA"
                     /note="color: #a949ca; direction: RIGHT"
                     /official_designation="BGH pA"
                     /uuid="4a09734ca6c745b3aadc757c1638b651"
     ITR             complement(3437..3577)
                     /application_notes="Allows rescue of virus from recombinant
                     plasmid and replication of the viral genome; this ITR is
                     identical to that of the wild-type AAV2 genome."
                     /description="AAV 3' inverted terminal repeat"
                     /label="3' ITR"
                     /note="color: #946a00; direction: LEFT"
                     /official_designation="3' ITR"
                     /uuid="5b8101593b634e64aa9eef9434216f71"
     CDS             4494..5354
                     /application_notes="Allows E. coli to be resistant to
                     ampiciIIin."
                     /description="AmpiciIIin resistance gene"
                     /full_name="Ampicillin resistance gene"
                     /label="AmpiciIIin"
                     /note="color: #512bbd; direction: RIGHT"
                     /official_designation="Ampicillin"
                     /uuid="5bcc09480c0e4c07b95ddccd8242d769"
     Rep_origin      5525..6113
                     /application_notes="Facilitates plasmid replication in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /description="pUC origin of replication"
                     /full_name="pUC origin of replication"
                     /label="pUC ori"
                     /note="color: #d84e4e; direction: RIGHT"
                     /official_designation="pUC ori"
                     /uuid="b978ad4ff785460db448caf63b728237"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttctagacaa ctttgtatag aaaagttgct cgacattgat
      181 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      241 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      301 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      361 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      421 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      481 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      541 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
      601 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
      661 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
      721 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
      781 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg
      841 cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
      901 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
      961 attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg
     1021 ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt
     1081 gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg
     1141 gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc
     1201 cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
     1261 ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc
     1321 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     1381 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     1441 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     1501 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     1561 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     1621 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     1681 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
     1741 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     1801 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     1861 tgctggttat tgtgctgtct catcattttg gcaaagaatt gcaagtttgt acaaaaaagc
     1921 aggctataac ttcgtatagg atactttata cgaagttatc ctcgagtacc ggtcacgcgt
     1981 acgcaagctt gataacttcg tatagcatac attatacgaa gttatcctag ggccacctca
     2041 gcccaccggc ttgccccagc ggaagtgctc catggagtag gagcgcttgc cctcgcgtgg
     2101 actcggctct ggactgccat ctccccacac cgcctcttcc tccgcacgcc tctgcgccgc
     2161 gctgccagca ctgctgctgt tcctggggcc gaagcggtcc cagcggaagt gacccatgac
     2221 gtacttccgg gggttttcag tcaggggctg ttcatctccg ttgccaggaa acacgggcgt
     2281 ctccagcgag aggtcgagtt tgcaagcccg gatgcaagcc agcaggttgc tctccgtggt
     2341 gaggtcctgg cactggctgc tctccaggca ccagctccac acatctatgg aggtctgaag
     2401 caggagggcc agcaacaggg cccctgagcg actgtagcag aatctcggca tggtggctct
     2461 agaataactt cgtataaagt atcctatacg aagttatagg tacctgagct caacatcgat
     2521 agcactagta taacttcgta taatgtatgc tatacgaagt tatacccagc tttcttgtac
     2581 aaagtgggaa ttccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     2641 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2701 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2761 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2821 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2881 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2941 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     3001 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     3061 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     3121 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     3181 ccccgcatcg ggaattccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     3241 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3301 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3361 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga atagcaggca
     3421 tgctggggag ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     3481 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     3541 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     3601 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     3661 gtagcggcgc attaagcgcg gcgggggtgg tggttacgcg cagcgtgacc gctacacttg
     3721 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3781 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3841 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     3901 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3961 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
     4021 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4081 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     4141 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4201 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4261 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     4321 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4381 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4441 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4501 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4561 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4621 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4681 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4741 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4801 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4861 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4921 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4981 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5041 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5101 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5161 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta
     5221 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5281 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5341 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5401 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5461 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5521 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5581 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5641 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5701 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5761 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5821 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5881 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5941 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6001 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6061 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6121 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
