LOCUS       pGP-pcDNA3.1_Puro-CAG-Positron2      7764 bp ds-DNA     circular UNA 
FEATURES             Location/Qualifiers
     intron          1..49
                     /note="chimeric intron"
                     /note="chimera between introns from chicken beta-actin
                     and rabbit beta-globin"
     CDS             215..1873
                     /label="Positron2"
     polyA_signal    1994..2218
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2264..2692
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2706..3035
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      2886..3021
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             3044..3643
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    3803..3924
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     protein_bind    3997..4013
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4021..4051)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4066..4087
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the
                     presence of cAMP."
     rep_origin      complement(4375..4960)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5131..5991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     promoter        complement(5992..6096)
                     /gene="bla"
                     /note="AmpR promoter"
     enhancer        6142..6521
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     intron          6797..7764
                     /note="chimeric intron"
                     /note="chimera between introns from chicken beta-actin
                     and rabbit beta-globin"
ORIGIN
        1 CTCTAGAGCC TCTGCTAACC ATGTTCATGC CTTCTTCTTT TTCCTACAGC TCCTGGGCAA
       61 CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCCTCGAGG AATTCACTCC
      121 TCAGGTGCAG GCTGCCTATC AGAAGGTGGT GGCTGGTGTG GCCAATGCCC TGGCTCACAA
      181 ATACCACTGA GATCTGCATG AGCTAGCCGC CACCATGGCT GACGTGGAAA CCGAGACCGG
      241 TATGATTGCA CAGTGGATTG TCTTTGCTAT TATGGCTGCT GCTGCTATTG CTTTTGGAGT
      301 GGCTGTGCAC TTTCGGCCTT CAGAGCTGAA GAGCGCATAC TATATCAACA TTGCCATCTG
      361 CACTATCGCC GCTACCGCTT ACTATGCAAT GGCCGTGAAC TACCAGGACC TGACAATGAA
      421 TGGTGAAAGG CAGGTGGTCT ACGCAAAATA TATTGACTGG GTGCTGACCA CACCACTGCT
      481 CCTGCTCAAC CTCATCGTCA TGACCAAGAT GGGCGGAGTG ATGATTTCTT GGGTCATCGG
      541 CGCAGACATT TTCATGATCG TGTTTGGTAT TCTGGGCGCC TTCGAGGATG AACACAAGTT
      601 CAAATGGGTG TACTTTATCG CTGGATGTGT GATGCAGGCA GTCCTGACAT ACGGGATGTA
      661 TAACGCCACT TGGAAAGACG ATCTGAAGAA AAGCCCCGAG TACCATAGCT CCTATGTCAG
      721 TCTGCTCGTC TTCCTGTCAA TCCTCTTCGT GTTTTATCCT GTCGTGTGGG CTTTCGGGTC
      781 TGGTAGTGGC GTGCTGTCCG TCGACAATGA GGCCATTCTC ATGGGAATCC TGGATGTGCT
      841 CGCTAAGCCA CTGTTTGGAA TGGGGTGCCT CATTGCCCAT GAGACTATCT TCAAGATCGG
      901 TACCGGCTTT CCATTCGACC CCCATTATGT GGAAGTCCTG GGCGAGCGCA TGCACTACGT
      961 CGATGTTGGT CCGCGCGATG GCACCCCTGT GCTGTTCCTG CACGGTAACC CGACCTCCTC
     1021 CTACGTGTGG CGCAACATCA TCCCGCATGT TGCACCGACC CATCGCTGCA TTGCTCCAGA
     1081 CCTGATCGGT ATGGGCAAAT CCGACAAACC AGACCTGGGT TATTTCTTCG ACGACCACGT
     1141 CCGCTTCATG GATGCCTTCA TCGAAGCCCT GGGTCTGGAA GAGGTCGTCC TGGTCATTCA
     1201 CGACTGGGGC TCCGCTCTGG GTTTCCACTG GGCCAAGCGC AATCCAGAGC GCGTCAAAGG
     1261 TATTGCATTT ATGGAGTTCA TCCGCCCTAT CCCGACCTGG GACGAATGGC CAGAATTTGC
     1321 CCGCGAGACC TTCCAGGCCT TCCGCACCAC CGACGTCGGC CGCAAGCTGA TCATCGATCA
     1381 GAACGTTTTT ATCGAGGGTA CGCTGCCGAT GGGTGTCGTC CGCCCGCTGA CTGAAGTCGA
     1441 GATGGACCAT TACCGCGAGC CGTTCCTGAA TCCTGTTGAC CGCGAGCCAC TGTGGCGCTT
     1501 CCCAAACGAG CTGCCAATCG CCGGTGAGCC AGCGAACATC GTCGCGCTGG TCGAAGAATA
     1561 CATGGACTGG CTGCACCAGT CCCCTGTCCC GAAGCTGCTG TTCTGGGGCA CCCCAGGCGT
     1621 TCTGATCCCA CCGGCCGAAG CCGCTCGCCT GGCCAAAAGC CTGCCTAACT GCAAGGCTGT
     1681 GGACATCGGC CCGGGTCTGA ATCTGCTGCA AGAAGACAAC CCGGACCTGA TCGGCAGCGA
     1741 GATCGCGCGC TGGCTGTCGA CGCTCGAGAT TTCCGGCGAG CCAACCACTA AGAGCAGGAT
     1801 CACCAGCGAG GGCGAGTACA TCCCCCTGGA CCAGATCGAC ATCAACGTGT TCTGCTACGA
     1861 GAACGAGGTG TAATGAAAGC TTCGTACCGA GCTCGGATCC ACTAGTCCAG TGTGGTGGAA
     1921 TTCTGCAGAT ATCCAGCACA GTGGCGGCCG CTCGAGTCTA GAGGGCCCGT TTAAACCCGC
     1981 TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG
     2041 CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT
     2101 GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC
     2161 AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT
     2221 TCTGAGGCGG AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC
     2281 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC
     2341 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC
     2401 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC
     2461 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG
     2521 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT
     2581 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT
     2641 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT
     2701 GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC
     2761 AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG
     2821 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC
     2881 CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA
     2941 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT
     3001 GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCATGACCG AGTACAAGCC
     3061 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC TCGCCGCCGC
     3121 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA TCGAGCGGGT
     3181 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
     3241 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC
     3301 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA
     3361 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC
     3421 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC
     3481 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG
     3541 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA
     3601 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGATTCGAAA TGACCGACCA
     3661 AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT
     3721 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT
     3781 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG
     3841 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT
     3901 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT
     3961 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
     4021 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT
     4081 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT
     4141 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC
     4201 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA
     4261 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
     4321 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
     4381 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     4441 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     4501 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     4561 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     4621 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     4681 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     4741 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     4801 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     4861 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT
     4921 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
     4981 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
     5041 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
     5101 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
     5161 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
     5221 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG
     5281 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
     5341 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
     5401 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
     5461 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
     5521 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
     5581 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
     5641 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
     5701 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
     5761 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
     5821 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
     5881 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
     5941 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
     6001 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     6061 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
     6121 TGACGTCGAC GGATCGGGGT CGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA
     6181 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG
     6241 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC
     6301 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA
     6361 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA
     6421 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA
     6481 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTCGAGGTG AGCCCCACGT
     6541 TCTGCTTCAC TCTCCCCATC TCCCCCCCCT CCCCACCCCC AATTTTGTAT TTATTTATTT
     6601 TTTAATTATT TTGTGCAGCG ATGGGGGCGG GGGGGGGGGG CGCGCGCCAG GCGGGGCGGG
     6661 GCGGGGCGAG GGGCGGGGCG GGGCGAGGCG GAGAGGTGCG GCGGCAGCCA ATCAGAGCGG
     6721 CGCGCTCCGA AAGTTTCCTT TTATGGCGAG GCGGCGGCGG CGGCGGCCCT ATAAAAAGCG
     6781 AAGCGCGCGG CGGGCGGGAG TCGCTGCGCG CTGCCTTCGC CCCGTGCCCC GCTCCGCCGC
     6841 CGCCTCGCGC CGCCCGCCCC GGCTCTGACT GACCGCGTTA CTCCCACAGG TGAGCGGGCG
     6901 GGACGGCCCT TCTCCTCCGG GCTGTAATTA GCGCTTGGTT TAATGACGGC TTGTTTCTTT
     6961 TCTGTGGCTG CGTGAAAGCC TTGAGGGGCT CCGGGAGGGC CCTTTGTGCG GGGGGAGCGG
     7021 CTCGGGGGGT GCGTGCGTGT GTGTGTGCGT GGGGAGCGCC GCGTGCGGCT CCGCGCTGCC
     7081 CGGCGGCTGT GAGCGCTGCG GGCGCGGCGC GGGGCTTTGT GCGCTCCGCA GTGTGCGCGA
     7141 GGGGAGCGCG GCCGGGGGCG GTGCCCCGCG GTGCGGGGGG GGCTGCGAGG GGAACAAAGG
     7201 CTGCGTGCGG GGTGTGTGCG TGGGGGGGTG AGCAGGGGGT GTGGGCGCGT CGGTCGGGCT
     7261 GCAACCCCCC CTGCACCCCC CTCCCCGAGT TGCTGAGCAC GGCCCGGCTT CGGGTGCGGG
     7321 GCTCCGTACG GGGCGTGGCG CGGGGCTCGC CGTGCCGGGC GGGGGGTGGC GGCAGGTGGG
     7381 GGTGCCGGGC GGGGCGGGGC CGCCTCGGGC CGGGGAGGGC TCGGGGGAGG GGCGCGGCGG
     7441 CCCCCGGAGC GCCGGCGGCT GTCGAGGCGC GGCGAGCCGC AGCCATTGCC TTTTATGGTA
     7501 ATCGTGCGAG AGGGCGCAGG GACTTCCTTT GTCCCAAATC TGTGCGGAGC CGAAATCTGG
     7561 GAGGCGCCGC CGCACCCCCT CTAGCGGGCG CGGGGCGAAG CGGTGCGGCG CCGGCAGGAA
     7621 GGAAATGGGC GGGGAGGGCC TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC TCCCTCTCCA
     7681 GCCTCGGGGC TGTCCGCGGG GGGACGGCTG CCTTCGGGGG GGACGGGGCA GGGCGGGGTT
     7741 CGGCTTCTGG CGTGTGACCG GCGG
//
