LOCUS       2E11                    6906 bp    DNA     circular SYN 01-MAY-2025
DEFINITION  2E11.
ACCESSION   2E11
VERSION     2E11
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             5876..6736
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(1017..1208)
                     /note="pLannotate"
                     /label="rop"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(775..915)
                     /note="pLannotate"
                     /label="bom"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             4609..5706
                     /note="pLannotate"
                     /label="MBP"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="99.7"
                     /fragment="False"
                     /other="CDS"
     promoter        5771..5875
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      complement(3507..3593)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      complement(1909..1995)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             complement(2433..3146)
                     /note="pLannotate"
                     /label="Superfolder GFP"
                     /database="fpbase"
                     /identity="98.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      complement(1790..1817)
                     /note="pLannotate"
                     /label="rrnB T2 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     ncRNA           complement(1997..2112)
                     /note="pLannotate"
                     /label="5S rRNA"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="97.5"
                     /fragment="False"
                     /other="ncRNA"
     CDS             3944..4534
                     /note="pLannotate"
                     /label="toxR (fragment)"
                     /database="swissprot"
                     /identity="93.4"
                     /match_length="67.0"
                     /fragment="True"
                     /other="CDS"
     CDS             1225..1605
                     /note="pLannotate"
                     /label="YPB1_ECOLX"
                     /database="swissprot"
                     /identity="99.2"
                     /match_length="96.2"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(3147..3175)
                     /note="pLannotate"
                     /label="cat promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="28.2"
                     /fragment="True"
                     /other="promoter"
     misc_feature    5707..5726
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.1"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(3326..3388)
                     /note="pLannotate"
                     /label="zot (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="5.3"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
     1081 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
     1141 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
     1201 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg
     1261 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa
     1321 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag
     1381 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
     1441 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt
     1501 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
     1561 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
     1621 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag
     1681 gacccaacgc tgcccgactg ctttcctgat gcaaaaacga ggctagttta ccgtatctgt
     1741 ggggggatgg cttgtagata tgacgacagg aagagtttgt agaaacgcaa aaaggccatc
     1801 cgtcaggatg gccttctgct taatttgatg cctggcagtt tatggcgggc gtcctgcccg
     1861 ccaccctccg ggccgttgct tcgcaacgtt caaatccgct cccggcggat ttgtcctact
     1921 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc
     1981 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac
     2041 catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc
     2101 tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat ttaatctgta
     2161 tcaggctgaa aatcttctct catccgccaa aacagccgag atgcgccgcg tgcggctgct
     2221 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct
     2281 ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgcct
     2341 cgacgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc aaccaggcgt
     2401 ttaagggcac caataactgc cttaaaaact catttgtaca gttcatccat accatgcgtg
     2461 atgcccgctg cggttacgaa ctccagcaga accatgtgat cgcgtttctc gttcggatct
     2521 ttagacagaa cgctttgcgt gctcagatag tgattgtctg gcagcagaac aggaccatca
     2581 ccgattggag tgttttgctg gtagtgatca gccagctgca cgctgccatc ctccacgttg
     2641 tggcgaattt taaaattcgc tttaatgcca tttttttgtt tatcggcggt gatgtaaaca
     2701 ttgtggctgt taaaattgta ttccagctta tggcccagga tattgccgtc ttctttaaag
     2761 tcaatgcctt tcagctcaat gcggtttacc agggtatcgc cttcaaattt cacttccgca
     2821 cgcgttttgt acgtgccgtc atccttaaag gaaatcgtgc gttcctgcac atagccttcc
     2881 ggcatggcgg acttgaagaa gtcatgctgc ttcatgtggt ccggataacg agcaaagcac
     2941 tgaacaccat aagtcagcgt cgttaccaga gtcggccaag gtaccggcag tttaccagta
     3001 gtacagatga acttcagcgt cagtttacca ttagttgcgt caccttcacc ctcgccacgc
     3061 acggaaaact tatgaccgtt gacatcacca tccagttcca ccagaatagg gacgacacca
     3121 gtgaacagct cttcgccttt gcccatttta gcttccttag ctcctgaaaa tctcgtcgaa
     3181 gctcggcgga tttgtcctac tcaagcttgc tccctttgtt taacagaaaa ataattgatc
     3241 aaaacaatta aataagtaaa taaatttgta ttatttgaaa tcaaaaatca aaaatcaaaa
     3301 atcaaaaatc aaaaatcaaa aatcaaaata tactatttag tcctttttta tcattttctg
     3361 tttttatcgg taaaccccgt ttcacttcta agcttggctg caggtcgacg gatcgatccc
     3421 cgggaattcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
     3481 gcaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
     3541 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg
     3601 gggaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     3661 ataataatgg tttcttaggg gcgcgtttct ttattagtgg ttgcagtctc gctcataatc
     3721 gctccgttta cttctgttcc aaacaattga tccattgaga ctcaatggaa ttaccttgat
     3781 gtgcaagtga gatatggaca aaaaatgtaa attcaaggtc aaaactcata aaaacactgt
     3841 tttttgatcg agattggatt attctaagtc tgcattttta tcaaagaaga taaaaaaacc
     3901 agtaaagtct gagtgttggg acagggagat actgggacat tagatgttcg gattaggaca
     3961 caactcaaaa gagatttcga tgagtcatat tggtactaaa ttcattcttg ctgaaaaatt
     4021 taccttcgat cccctaagca atactctgat tgacaaagaa gatagtgaag agatcattcg
     4081 attaggcagc aacgaaagcc gaattctttg gctgctggcc caacgtccaa acgaggtgat
     4141 ttctcgcaat gatttgcatg actttgtttg gcgagagcaa ggttttgaag tcgatgattc
     4201 cagcttaacc caagccattt cgactctgcg caaaatgctc aaagattcga caaagtcccc
     4261 acaatacgtc aaaacggttc cgaagcgcgg ttaccaattg atcgcccgag tggaaacggt
     4321 tgaagaagag atggctcgcg aaaacgaagc tgctcatgac atctctcagc cagaatctgt
     4381 caatgaatac gcagaatcaa gcagtgtgcc ttcatcagcc actgtagtga acacaccgca
     4441 gccagccaat gtcgtggcga ataaatcggc tccaaacttg gggaatcgag ctagctatat
     4501 tgcgattggc attgacgtgg gcctgctgct gctgggcttt ctggtgattt ttattgcttg
     4561 atcctgatca acccaagcca atccagcttt aaacccctaa cggatatcaa aatcgaagaa
     4621 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
     4681 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
     4741 gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac
     4801 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
     4861 ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt
     4921 gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac
     4981 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
     5041 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
     5101 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
     5161 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
     5221 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
     5281 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
     5341 acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca
     5401 ggtattaacg ccgccagtcc gaacaaagag ctggcgaaag agttcctcga aaactatctg
     5461 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
     5521 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccaccat ggaaaacgcc
     5581 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt
     5641 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
     5701 cagactaatt cgagctcggt acccggccgg ggatcttaat ctagcaggtg gcacttttcg
     5761 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5821 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5881 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5941 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6001 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6061 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     6121 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6181 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6241 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6301 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6361 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     6421 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6481 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6541 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6601 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6661 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6721 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6781 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6841 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6901 atcttc
//
