LOCUS       pBL098_-_[p15a]_PTac_SD 3529 bp ds-DNA     circular     18-SEP-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          complement(13..31)
                     /label="DM0112"
                     /note="sequence: cttcctggcatcttccagg"
     primer          complement(55..71)
                     /label="DM0097"
                     /note="sequence: aacggctttgccgcggc"
     prim_transcript 72..72
                     /label="prim_transcript"
     promoter        433..472
                     /label="RNA I Promoter (0.96)"
     -35_signal      438..443
                     /label="-35 -35 signal"
     -10_signal      462..467
                     /label="-10 -10 signal"
     misc_RNA        474..577
                     /label="RNA I"
     primer          534..554
                     /label="TW2019"
                     /note="sequence: CAGAGAACCTTCGAAAAACCG"
     primer          534..554
                     /label="TW2019"
                     /note="sequence: CAGAGAACCTTCGAAAAACCG"
     promoter        complement(584..623)
                     /label="RNA II Promoter (0.92)"
     -10_signal      complement(589..594)
                     /label="-10 -10 signal"
     primer          599..621
                     /label="TW2020"
                     /note="sequence: cagaccaaaacgatctcaagaag"
     primer          599..621
                     /label="TW2020"
                     /note="sequence: cagaccaaaacgatctcaagaag"
     -35_signal      complement(612..617)
                     /label="-35 -35 signal"
     primer          complement(641..663)
                     /label="TW2311"
                     /note="sequence: ATGTCCTCTcgtatatcgtatgg"
     terminator      668..713
                     /label="Modded to remove elements that cause sigma70binding sites to arise with new binding sites"
     primer          complement(696..728)
                     /label="BL0190"
                     /note="sequence: agggtccctaagtctgcaacgaaaggcccagtc"
     USER junction   714..728
                     /label="USER008"
     primer          714..746
                     /label="TW2133"
                     /note="sequence: agacttagggacccTtcactgcccgctttccag"
     CDS             complement(729..1811)
                     /label="lacI"
     primer          complement(750..767)
                     /label="TW2106"
                     /note="sequence: ctggcacgacaggtttcc"
     primer          1176..1193
                     /label="TW2162"
                     /note="sequence: CGGCTGAATTTGATTGCG"
     primer          complement(1181..1207)
                     /label="BL0073"
                     /note="sequence: ataaatatctcactcgcaatcaaattc"
     primer          complement(1379..1396)
                     /label="TW2105"
                     /note="sequence: cctgcactaatgttccgg"
     promoter        complement(1812..1889)
                     /label="lacIq promoter"
     primer          1822..1841
                     /label="TW2163"
                     /note="sequence: CTGAATTGACTCTCTTCCGG"
     primer          complement(1872..1907)
                     /label="TW2134"
                     /note="sequence: acattaattgcgtTgcgcgacaccatcgaatggtgc"
     USER junction   1894..1907
                     /label="USER"
     primer          1894..1919
                     /label="TW2135"
                     /note="sequence: aacgcaattaatgTaagttagctcac"
     primer          2093..2111
                     /label="TW2406"
                     /note="sequence: acatcataacggttctggc"
     promoter        2132..2160
                     /label="tac promoter"
     operator        2164..2186
                     /label="lacO"
     primer          2182..2202
                     /label="TW2427"
                     /note="sequence: caatttcacacaggaaacagc"
     primer          complement(2222..2251)
                     /label="TW2136"
                     /note="sequence: actgcaccTattgttggtgaagtgctcgtg"
     USER junction   2243..2251
                     /label="USER009"
     primer          2243..2263
                     /label="BL0227"
                     /note="sequence: AGGTGCAGTaaggaggaaaaa"
     primer          2243..2269
                     /label="JA0045"
                     /note="sequence: aggtgcagtaaggaggaaaaaaaaatg"
     primer          2243..2269
                     /label="TW2035"
                     /note="sequence: aggtgcagtaaggaggaaaaaaaaATG"
     primer          2243..2269
                     /label="TW2035"
                     /note="sequence: aggtgcagtaaggaggaaaaaaaaATG"
     misc_feature    complement(2248..2251)
                     /label="gg junction"
     primer          2249..2287
                     /label="BL0757"
                     /note="sequence: agtAAGGAGGAAAAAAAAatgaaaagcccggaagaacta"
     RBS             2252..2266
                     /label="SD8"
     misc_feature    2267..2536
                     /label="SXkmer-loop YLTIRLM"
     CDS             2267..2536
                     /label="Translation 2267-2536"
                     /translation="MKSPEELKRIFEKYAAKEGDPDQLSKDELKLLIQAEFPSLLKGMGGGYLTIRLMGGGSTLDDLFQELDKDGDGEVSFEEFQVLVKKISQ*"
     primer          2363..2381
                     /label="BL0738"
                     /note="sequence: atccaggccgaatttcctt"
     primer          complement(2387..2404)
                     /label="BL0723"
                     /note="sequence: accgcccatgccctttaa"
     misc_feature    2396..2404
                     /label="USER"
     misc_feature    2408..2428
                     /label="YLTIRLM"
     primer          complement(2485..2502)
                     /label="BL0739"
                     /note="sequence: tcttcgaagctgacctcc"
     primer          complement(2510..2548)
                     /label="BL0570"
                     /note="sequence: agtgccgttaatttactgactaattttcttaaccagcac"
     primer          complement(2520..2548)
                     /label="BL0846"
                     /note="sequence: AGTGCCGTTAATttactgactaattttct"
     misc_feature    2537..2540
                     /label="gg junction"
     USER junction   2537..2548
                     /label="USER002"
     primer          2537..2558
                     /label="TW2007"
                     /note="sequence: ATTAACGGCACTGCGAAAAAAC"
     primer          2537..2558
                     /label="TW2007"
                     /note="sequence: ATTAACGGCACTGCGAAAAAAC"
     terminator      complement(2549..2582)
                     /label="BBa_B1002 terminator"
     terminator      complement(2583..2624)
                     /label="tetA/orfL bidirectional terminator"
     primer          complement(2617..2637)
                     /label="JA0006"
                     /note="sequence: acgccatgggcattaaaaaat"
     USER junction   2625..2637
                     /label="USER005"
     primer          2625..2641
                     /label="TW2309"
                     /note="sequence: ATGCCCATGGCGTacgg"
     primer_bind     2625..2671
                     /label="TW0738"
     CDS             complement(2645..3460)
                     /label="KanR"
     primer          complement(2771..2790)
                     /label="TW2018"
                     /note="sequence: ACCGATACCAGGATCTTGCC"
     primer          complement(2771..2790)
                     /label="TW2018"
                     /note="sequence: ACCGATACCAGGATCTTGCC"
     primer          complement(2892..2914)
                     /label="TW2025"
                     /note="sequence: cataaacttttgccattctcacc"
     primer          complement(2892..2914)
                     /label="TW2025"
                     /note="sequence: cataaacttttgccattctcacc"
     promoter        complement(3461..3511)
                     /label="KanR promoter"
     misc_feature    3477..3521
                     /label="pr"
     primer          3512..16
                     /label="TW2156"
                     /note="sequence: agagaaaccTacggatcggggcggagatttcctg"
     USER junction   3512..3521
                     /label="USER013"
ORIGIN
        1 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
       61 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
      121 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
      181 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      241 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
      301 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
      361 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
      421 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
      481 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
      541 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
      601 gaccaaaacg atctcaagaa gatcatctta ttaaggagcc ccatacgata tacgagagga
      661 catccgggca aatgcaacga aaggctcagt cgaaagactg ggcctttcgt tgcagactta
      721 gggacccttc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      781 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc
      841 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
      901 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
      961 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca
     1021 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
     1081 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
     1141 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
     1201 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
     1261 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
     1321 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
     1381 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
     1441 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
     1501 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
     1561 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
     1621 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
     1681 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
     1741 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
     1801 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
     1861 cgaaaggttt tgcaccattc gatggtgtcg cgcaacgcaa ttaatgtaag ttagctcact
     1921 cattaggcac aattctcatg tttgacagct tatcatcgac tgcacggtgc accaatgctt
     1981 ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat
     2041 aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata
     2101 acggttctgg caaatattct gaaatgagct gttgacaatt aatcatcggc tcgtataatg
     2161 tgtggaattg tgagcggata acaatttcac acaggaaaca gccagtccgt ttaggtgttt
     2221 tcacgagcac ttcaccaaca atAGGTGCAG Taaggaggaa aaaaaaatga aaagcccgga
     2281 agaactaaaa cgcatcttcg aaaaatatgc ggcaaaggag ggtgacccgg atcaactttc
     2341 gaaagatgaa ctgaaactgc tgatccaggc cgaatttcct tcactcttaa agggcatggg
     2401 cggtggctac ctgaccattc gtcttatggg aggtggctct acgttggacg atttatttca
     2461 ggagctggac aaagatggcg atggggaggt cagcttcgaa gaatttcaag tgctggttaa
     2521 gaaaattagt cagtaaATTA ACGGCACTgc gaaaaaaccc cgccgaagcg gggttttttg
     2581 cgaaatataa tgaccctctt gataacccaa gagggcattt tttaatgccc atggcgtacg
     2641 gatcttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     2701 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     2761 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     2821 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     2881 cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag
     2941 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     3001 attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     3061 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     3121 gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg
     3181 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     3241 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     3301 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
     3361 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     3421 gcggcctaga gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt
     3481 ttatgtaagc agacagtttt attgttcatg aagagaaacc tacggatcg
//