LOCUS       pBL108Z_-_[p15a]_PTac_S 3745 bp ds-DNA     circular     06-FEB-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          complement(13..31)
                     /label="DM0112"
                     /note="sequence: cttcctggcatcttccagg"
     primer          complement(55..71)
                     /label="DM0097"
                     /note="sequence: aacggctttgccgcggc"
     prim_transcript 72..72
                     /label="prim_transcript"
     promoter        433..472
                     /label="RNA I Promoter (0.96)"
     -35_signal      438..443
                     /label="-35 -35 signal"
     -10_signal      462..467
                     /label="-10 -10 signal"
     misc_RNA        474..577
                     /label="RNA I"
     primer          534..554
                     /label="TW2019"
                     /note="sequence: CAGAGAACCTTCGAAAAACCG"
     primer          534..554
                     /label="TW2019"
                     /note="sequence: CAGAGAACCTTCGAAAAACCG"
     promoter        complement(584..623)
                     /label="RNA II Promoter (0.92)"
     -10_signal      complement(589..594)
                     /label="-10 -10 signal"
     primer          599..621
                     /label="TW2020"
                     /note="sequence: cagaccaaaacgatctcaagaag"
     primer          599..621
                     /label="TW2020"
                     /note="sequence: cagaccaaaacgatctcaagaag"
     -35_signal      complement(612..617)
                     /label="-35 -35 signal"
     primer          complement(641..663)
                     /label="TW2311"
                     /note="sequence: ATGTCCTCTcgtatatcgtatgg"
     terminator      668..713
                     /label="Modded to remove elements that cause sigma70binding sites to arise with new binding sites"
     primer          complement(696..728)
                     /label="BL0190"
                     /note="sequence: agggtccctaagtctgcaacgaaaggcccagtc"
     USER junction   714..728
                     /label="USER008"
     primer          714..746
                     /label="TW2133"
                     /note="sequence: agacttagggacccTtcactgcccgctttccag"
     CDS             complement(729..1811)
                     /label="lacI"
     primer          complement(750..767)
                     /label="TW2106"
                     /note="sequence: ctggcacgacaggtttcc"
     primer          1176..1193
                     /label="TW2162"
                     /note="sequence: CGGCTGAATTTGATTGCG"
     primer          complement(1181..1207)
                     /label="BL0073"
                     /note="sequence: ataaatatctcactcgcaatcaaattc"
     primer          complement(1379..1396)
                     /label="TW2105"
                     /note="sequence: cctgcactaatgttccgg"
     promoter        complement(1812..1889)
                     /label="lacIq promoter"
     primer          1822..1841
                     /label="TW2163"
                     /note="sequence: CTGAATTGACTCTCTTCCGG"
     primer          complement(1852..1878)
                     /label="JW0063"
                     /note="sequence: atggtgcaaaacctttcgcggtatggc"
     primer          1865..1894
                     /label="JW0062"
                     /note="sequence: aggttttgcaccattcgatggtgtcgcgca"
     primer          complement(1872..1907)
                     /label="TW2134"
                     /note="sequence: acattaattgcgtTgcgcgacaccatcgaatggtgc"
     USER junction   1894..1907
                     /label="USER"
     primer          1894..1919
                     /label="TW2135"
                     /note="sequence: aacgcaattaatgTaagttagctcac"
     primer          2093..2111
                     /label="TW2406"
                     /note="sequence: acatcataacggttctggc"
     promoter        2132..2160
                     /label="tac promoter"
     operator        2164..2186
                     /label="lacO"
     primer          2182..2202
                     /label="TW2427"
                     /note="sequence: caatttcacacaggaaacagc"
     primer          complement(2222..2251)
                     /label="TW2136"
                     /note="sequence: actgcaccTattgttggtgaagtgctcgtg"
     USER junction   2243..2251
                     /label="USER009"
     primer          2243..2263
                     /label="BL0227"
                     /note="sequence: AGGTGCAGTaaggaggaaaaa"
     primer          2243..2269
                     /label="TW2035"
                     /note="sequence: aggtgcagtaaggaggaaaaaaaaATG"
     primer          2243..2269
                     /label="JA0045"
                     /note="sequence: aggtgcagtaaggaggaaaaaaaaatg"
     primer          2243..2269
                     /label="TW2035"
                     /note="sequence: aggtgcagtaaggaggaaaaaaaaATG"
     primer          2243..2284
                     /label="BL0720"
                     /note="sequence: AGGTGCAGTaaggaggaaaaaaaaATGCGTGGTTCGCATCAC"
     misc_feature    complement(2248..2251)
                     /label="gg junction"
     RBS             2252..2266
                     /label="SD8"
     misc_feature    2267..2752
                     /label="DesABO"
     CDS             2267..2752
                     /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDPKLEVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIGWVRRAPGKGEEWVASIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCAAGSESAFGRAEEEAAAWGQGTLVTVSSGT*"
     primer          complement(2347..2369)
                     /label="BL1010"
                     /note="sequence: ACTTTGGATCTTTGTCATCGTCG"
     primer          2359..2391
                     /label="BL1009"
                     /note="sequence: AGATCCAAAGTTGGAGGTACAGTTAGTGGAGTC"
     primer          2371..2430
                     /label="BL1005"
                     /note="sequence: GGAGGTACAGTTAGTGGAGTCCGGTGGGGGCCTGGTTCAGCCAGGGGGGTCCTTGCGTCT"
     primer          2420..2437
                     /label="BL1034"
                     /note="sequence: TCCTTGCGTCTGAGTTGC"
     misc_feature    2462..2476
                     /label="CDR1"
     primer          2471..2489
                     /label="BL1074"
                     /note="sequence: ATCGGCTGGGTACGTCGTG"
     misc_feature    2477..2518
                     /label="FR2"
     misc_feature    2519..2569
                     /label="CDR2"
     primer          complement(2549..2596)
                     /label="BL1006"
                     /note="sequence: GGATGTGTCAGCGCTAATAGTGAAACGACCTTTGACAGAGTCGGCATA"
     primer          complement(2571..2591)
                     /label="BL1035"
                     /note="sequence: TGTCAGCGCTAATAGTGAAAC"
     primer          2575..2616
                     /label="BL1011"
                     /note="sequence: CACTATTAGCGCTGACACATCCAAGAACACCGCGTATCTTCA"
     primer          2576..2606
                     /label="tr2"
                     /note="sequence: ACTATTAGCGCTGACACATCCAAGAACACCG"
     primer          complement(2576..2606)
                     /label="trash412"
                     /note="sequence: CGGTGTTCTTGGATGTGTCAGCGCTAATAGT"
     primer          2588..2607
                     /label="tr3"
                     /note="sequence: GACACATCCAAGAACACCGC"
     primer          2594..2612
                     /label="BL1036"
                     /note="sequence: TCCAAGAACACCGCGTATC"
     primer          2597..2643
                     /label="BL1007"
                     /note="sequence: AAGAACACCGCGTATCTTCAAATGAACAGCTTACGTGCCGAGGATAC"
     primer          2602..2623
                     /label="Trash412321412"
                     /note="sequence: CACCGCGTATCTTCAAATGAAC"
     primer          complement(2654..2671)
                     /label="BL0787"
                     /note="sequence: CGACCCGGCCGCGCAGTA"
     primer          complement(2663..2680)
                     /label="BL0790"
                     /note="sequence: GGCACTTTCCGACCCGGC"
     misc_feature    2672..2692
                     /label="CDR3"
     primer          complement(2693..2752)
                     /label="BL1008"
                     /note="sequence: TTATGTTCCCGAAGAAACAGTTACTAAAGTGCCTTGGCCCCATGCGGCTGCTTCCTCTTC"
     primer          complement(2693..2752)
                     /label="tr5"
                     /note="sequence: TTATGTTCCCGAAGAAACAGTTACTAAAGTGCCTTGGCCCCATGCGGCTGCTTCCTCTTC"
     primer          2701..2717
                     /label="BL1076"
                     /note="sequence: AGCAGCCGCATGGGGCC"
     primer          complement(2715..2735)
                     /label="BL1037"
                     /note="sequence: CAGTTACTAAAGTGCCTTGGC"
     primer          complement(2729..2764)
                     /label="BL0721"
                     /note="sequence: AGTGCCGTTAATTTATGTTCCCGAAGAAACAGTTAC"
     misc_feature    2753..2756
                     /label="gg junction"
     USER junction   2753..2764
                     /label="USER002"
     primer          2753..2774
                     /label="TW2007"
                     /note="sequence: ATTAACGGCACTGCGAAAAAAC"
     primer          2753..2774
                     /label="TW2007"
                     /note="sequence: ATTAACGGCACTGCGAAAAAAC"
     terminator      complement(2765..2798)
                     /label="BBa_B1002 terminator"
     terminator      complement(2799..2840)
                     /label="tetA/orfL bidirectional terminator"
     primer          complement(2833..2853)
                     /label="JA0006"
                     /note="sequence: acgccatgggcattaaaaaat"
     USER junction   2841..2853
                     /label="USER005"
     primer          2841..2857
                     /label="TW2309"
                     /note="sequence: ATGCCCATGGCGTacgg"
     primer          2841..2860
                     /label="Trash4124"
                     /note="sequence: atgcccatggcgtacggatc"
     primer_bind     2841..2887
                     /label="TW0738"
     CDS             complement(2861..3676)
                     /label="KanR"
     primer          complement(2987..3006)
                     /label="TW2018"
                     /note="sequence: ACCGATACCAGGATCTTGCC"
     primer          complement(2987..3006)
                     /label="TW2018"
                     /note="sequence: ACCGATACCAGGATCTTGCC"
     primer          complement(3108..3130)
                     /label="TW2025"
                     /note="sequence: cataaacttttgccattctcacc"
     primer          complement(3108..3130)
                     /label="TW2025"
                     /note="sequence: cataaacttttgccattctcacc"
     promoter        complement(3677..3727)
                     /label="KanR promoter"
     misc_feature    3693..3737
                     /label="pr"
     primer          3728..16
                     /label="TW2156"
                     /note="sequence: agagaaaccTacggatcggggcggagatttcctg"
     USER junction   3728..3737
                     /label="USER013"
ORIGIN
        1 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
       61 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
      121 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
      181 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      241 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
      301 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
      361 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
      421 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
      481 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
      541 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
      601 gaccaaaacg atctcaagaa gatcatctta ttaaggagcc ccatacgata tacgagagga
      661 catccgggca aatgcaacga aaggctcagt cgaaagactg ggcctttcgt tgcagactta
      721 gggacccttc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      781 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc
      841 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
      901 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
      961 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca
     1021 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
     1081 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
     1141 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
     1201 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
     1261 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
     1321 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
     1381 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
     1441 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
     1501 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
     1561 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
     1621 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
     1681 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
     1741 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
     1801 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
     1861 cgaaaggttt tgcaccattc gatggtgtcg cgcaacgcaa ttaatgtaag ttagctcact
     1921 cattaggcac aattctcatg tttgacagct tatcatcgac tgcacggtgc accaatgctt
     1981 ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat
     2041 aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata
     2101 acggttctgg caaatattct gaaatgagct gttgacaatt aatcatcggc tcgtataatg
     2161 tgtggaattg tgagcggata acaatttcac acaggaaaca gccagtccgt ttaggtgttt
     2221 tcacgagcac ttcaccaaca atAGGTGCAG Taaggaggaa aaaaaaATGC GTGGTTCGCA
     2281 TCACCATCAT CATCACGGTA TGGCATCAAT GACGGGCGGT CAGCAAATGG GTCGTGATTT
     2341 GTATGACGAC GATGACAAAG ATCCAAAGTT GGAGGTACAG TTAGTGGAGT CCGGTGGGGG
     2401 CCTGGTTCAG CCAGGGGGGT CCTTGCGTCT GAGTTGCGCG GCATCCGGCT TCAACATCAA
     2461 AGACACTTAC ATCGGCTGGG TACGTCGTGC TCCTGGGAAG GGCGAGGAGT GGGTAGCCTC
     2521 TATTTACCCC ACTAATGGGT ACACTCGTTA TGCCGACTCT GTCAAAGGTC GTTTCACTAT
     2581 TAGCGCTGAC ACATCCAAGA ACACCGCGTA TCTTCAAATG AACAGCTTAC GTGCCGAGGA
     2641 TACAGCGGTT TACTACTGCG CGGCCGGGTC GGAAAGTGCC TTTGGACGCG CTGAAGAGGA
     2701 AGCAGCCGCA TGGGGCCAAG GCACTTTAGT AACTGTTTCT TCGGGAACAT AAATTAACGG
     2761 CACTgcgaaa aaaccccgcc gaagcggggt tttttgcgaa atataatgac cctcttgata
     2821 acccaagagg gcatttttta atgcccatgg cgtacggatc ttagaaaaac tcatcgagca
     2881 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     2941 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     3001 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     3061 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     3121 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     3181 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     3241 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     3301 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     3361 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     3421 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     3481 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     3541 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     3601 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc
     3661 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     3721 ttcatgaaga gaaacctacg gatcg
//