LOCUS       pLS413                  3181 bp    DNA     circular SYN 12-MAR-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Lorenzo Scutteri
  TITLE     Direct Submission
  JOURNAL   Exported Mar 12, 2025 from SnapGene 8.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3181
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        1..64
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             65..925
                     /codon_start=1
                     /label=AmpR
                     /label=ampicillin resistance
                     /label=ampicillin\resistance
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    926..947
                     /label=AmpR ter
                     /note="color: #ffffff"
     rep_origin      948..1536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    1537..2040
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
                     /note="color: #454f59"
     primer_bind     2041..2057
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    2058..2113
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
                     /note="color: #a6acb3"
     primer_bind     complement(2114..2130)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     promoter        2131..2318
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 pr
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2319..2981
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /note="color: #ff6600"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     misc_feature    2982..3181
                     /label=HIS3 3'-UTR
                     /note="color: #ffff99"
ORIGIN
        1 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
       61 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgcagaag atcagttggg
      181 tgcgcgagtg ggttacatcg aactggacct caacagtggt aagatactag agagttttcg
      241 ccccgaagaa cgattcccaa tgatgtccac ttttaaggtt ctgctatgtg gcgcggtatt
      301 atcccgtatt gacgcggggc aagagcaact aggtcgccgc atacactatt ctcagaatga
      361 cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      421 attatgtagt gctgccataa cgatgagtga taacacggcg gcgaacttac ttctgacaac
      481 gattggagga ccaaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      541 ccttgatcgt tgggaaccag agctgaatga agccatacca aacgacgagc gtgacaccac
      601 gatgcctgtt gctatggcaa caaccttgcg gaaactatta actggcgaac tacttactct
      661 agcttcccgg caacagttaa tagactggat ggaggctgat aaagttgcag gaccacttct
      721 gcgctctgcg cttccggctg gctggtttat tgctgataaa tctggggccg gagagcgtgg
      781 gtcacgcggt atcatagcag cactagggcc agatggtaag ccctcccgta tcgtagttat
      841 ctacacgacg gggagccagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      901 tgcgtcactg attaagcatt ggtagtagaa aagatcaaag gatcttcttg agatcctttt
      961 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1021 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1081 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1141 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1501 ttgtgatgct cgtcaggggg gcggagccta tggaaaatca cgtgctataa aaataattat
     1561 aatttaaatt ttttaatata aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa
     1621 agaaaaaata gtttttgttt tccgaagatg taaaagactc tagggggatc gccaacaaat
     1681 actacctttt atcttgctct tcctgctctc aggtattaat gccgaattgt ttcatcttgt
     1741 ctgtgtagaa gaccacacac gaaaatcctg tgattttaca ttttacttat cgttaatcga
     1801 atgtatatct atttaatctg cttttcttgt ctaataaata tatatgtaaa gtacgctttt
     1861 tgttgaaatt ttttaaacct ttgtttattt ttttttcttc attccgtaac tcttctacct
     1921 tctttattta ctttctaaaa tccaaataca aaacataaaa ataaataaac acagagtaaa
     1981 ttcccaaatt attccatcat taaaagatac gaggcgcgtg taagttacag gcaagcgatc
     2041 caggaaacag ctatgacgcg cgcgagctcg gtacccggga tcctctagag tcgacctgca
     2101 ggcatgccct aggactggcc gtcgttttac ctagtacact ctatattttt ttatgcctcg
     2161 gtaatgattt tcattttttt ttttccacct agcggatgac tctttttttt tcttagcgat
     2221 tggcattatc acataatgaa ttatacatta tataaagtaa tgtgatttct tcgaagaata
     2281 tactaaaaaa tgagcaggca agataaacga aggcaaagat gacagagcag aaagccctag
     2341 taaagcgtat tacaaatgaa accaagattc agattgcgat ttctttaaag ggtggtcccc
     2401 tagcgataga acactcgatc ttcccagaaa aagaggcaga agcagtagca gaacaggcca
     2461 cacaatcgca agtgattaac gtccacacag gtatagggtt tctggaccac atgattcatg
     2521 ctctcgccaa gcactccggc tggtcgctaa tcgttgagtg cattggtgac ttacacatag
     2581 acgaccatca caccactgag gactgcggga ttgctctggg tcaggctttt aaagaggctc
     2641 taggggccgt gcgtggagta aaaaggtttg gatcaggatt tgcgcctttg gatgaggcac
     2701 tttccagggc ggttgttgat ctttcgaaca ggccatacgc agttgtcgaa cttggtttgc
     2761 aaagggagaa agtaggtgat ctctcttgcg agatgatccc gcattttctt gaaagttttg
     2821 cagaggcaag cagaattacc ctccacgttg attgtctgcg aggcaagaat gaccatcacc
     2881 gtagcgagag tgcgttcaag gctcttgcgg ttgccataag agaagccacc tcgcccaatg
     2941 gaaccaacga tgttccctcc accaaaggtg ttcttatgta gtgacaccga ttatttaaag
     3001 ctgcatcata cgatatatat atatgtgtat atatgtatat ctatgaatgt cagtaagtat
     3061 gtatatgaac agtatgatac tgaaaatgac aaggtaatac atcattctat atgtgtcatt
     3121 ctgaacgagg cgcgttttcc ttttttcttt ttgctttttc tttttttttc tcttgaactc
     3181 g
//
