LOCUS       1406_CDK11Bp58_G        9055 bp DNA     circular SYN 21-APR-2025
DEFINITION  1406_CDK11Bp58_G579S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 21, 2025 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
COMMENT     Sequence Label: pLenti CMV CDK11B, G579S-p58 Expression Clone
FEATURES             Location/Qualifiers
     source          1..9055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 913 .. 918 / #ffffff / -35
                        2: 919 .. 936 / #ffffff
                        3: 937 .. 943 / #ffffff / -10"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     enhancer        2998..3301
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        3302..3505
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    complement(4904..5028)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #31849b; direction: LEFT"
     misc_feature    5059..5647
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(5530..5541)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 5529"
                     /translation="IEGR"
     promoter        5703..6032
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5883..6018
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        6080..6127
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6146..6544
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /note="color: #ccffcc"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6631..6864
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    6936..7057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      7097..7232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        complement(7253..7271)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(7281..7297)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      7439..7894
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        7920..8024
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             join(8025..8093,8094..8885)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8025 .. 8093 / #ccffcc / signal sequence
                        2: 8094 .. 8885 / #ccffcc
                     Cleavage site after base 8093"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaattcaaa attttatcga taagcttggg agttccgcgt
     3001 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     3061 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3121 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     3181 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     3241 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     3301 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     3361 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     3421 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     3481 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     3541 tccacgctgt tttgacctcc atagaagaca ccgactcatg agtgaagatg aagaacgaga
     3601 aaatgaaaac cacctcttgg ttgttccaga gtcacggttc gaccgagatt ccggggagag
     3661 tgaagaagca gaggaagaag tgggtgaggg aacgccgcag agcagcgccc tgacagaggg
     3721 cgactatgtg cccgactccc ctgccctgtc gcccatcgag ctcaagcagg agctgcccaa
     3781 gtacctgccg gccctgcagg gctgccggag cgtcgaggag ttccagtgcc tgaacaggat
     3841 cgaggagggc acctatggag tggtctacag agcaaaagac aagaaaacag atgaaattgt
     3901 ggctctaaag cggctgaaga tggagaagga gaaggagggc ttcccgatca cgtcgctgag
     3961 ggagatcaac accatcctca aggcccagca tcccaacatc gtcaccgtta gagagattgt
     4021 ggtgggcagc aacatggaca agatctacat cgtgatgaac tatgtggagc acgacctcaa
     4081 gagcctgatg gagaccatga aacagccctt cctgccaggg gaggtgaaga ccctgatgat
     4141 ccagctgctg cgtggggtga aacacctgca cgacaactgg atcctgcacc gtgacctcaa
     4201 gacgtccaac ctgctgctga gccacgccgg catcctcaag gtgagtgact tcgggctggc
     4261 gcgggagtac ggatcccctc tgaaggccta caccccggtc gtggtgaccc tgtggtaccg
     4321 cgccccagag ctgctgcttg gtgccaagga atactccacg gccgtggaca tgtggtcagt
     4381 gggttgcatc ttcggggagc tgctgactca gaagcctctg ttccccggga agtcagaaat
     4441 cgatcagatc aacaaggtgt tcaaggatct ggggacccct agtgagaaaa tctggcccgg
     4501 ctacagcgag ctcccagcag tcaagaagat gaccttcagc gagcacccct acaacaacct
     4561 ccgcaagcgc ttcggggctc tgctctcaga ccagggcttc gacctcatga acaagttcct
     4621 gacctacttc cccgggagga ggatcagcgc tgaggacggc ctcaagcatg agtatttccg
     4681 cgagaccccc ctccccatcg acccctccat gttccccacg tggcccgcca agagcgagca
     4741 gcagcgtgtg aagcggggca ccagcccgag gccccctgag ggaggcctgg gctacagcca
     4801 gctgggtgac gacgacctga aggagacggg cttccacctt accaccacga accagggggc
     4861 ctctgccgcg ggccccggct tcagcctcaa gttctgaatc gaccatagtg actggatatg
     4921 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     4981 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg atatccagca
     5041 cagtggcggc cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5101 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5161 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5221 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5281 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5341 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5401 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     5461 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5521 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5581 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5641 tccccgcctg gaattctgca gatatccggt tagtaatgag tttggaatta attctgtgga
     5701 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5761 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     5821 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5881 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5941 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     6001 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     6061 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     6121 atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag aatccaccct
     6181 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     6241 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     6301 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     6361 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     6421 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     6481 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     6541 ctaagcacaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     6601 ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc caacgaagac
     6661 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6721 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6781 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6841 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     6901 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     6961 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7021 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     7081 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7141 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7201 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     7261 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     7321 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     7381 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     7441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7621 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7741 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7801 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7861 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     7921 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7981 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8041 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8101 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8161 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8221 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     8281 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8341 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8401 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8461 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8521 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8581 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8641 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8701 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8761 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8821 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8881 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
