LOCUS       p4919                   9207 bp    DNA     circular SYN 03-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 28, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    61..410
                     /label=alpha to beta Tub inter ORF
     CDS             411..2690
                     /codon_start=1
                     /label=Os CUL1/CDC53
                     /translation="MATHERKTIDLEQGWEFMQKGITKLKNILEGKPEPQFSSEDYMML
                     YTTIYNMCTQKPPHDYSQQLYEKYRESFEEYITSMVLPSLREKHDEFMLRELVKRWSNH
                     KVMVRWLSRFFHYLDRYFISRRSLPQLSEVGLSCFRDLVYQEIKGKVKSAVISLIDQER
                     EGEQIDRALLKNVLDIFVEIGLTSMDYYENDFEDFLLKDTADYYSIKAQTWILEDSCPD
                     YMLKAEECLKREKERVAHYLHSSSEQKLLEKVQHELLTQYASQLLEKEHSGCHALLRDD
                     KVDDLSRMYRLFSRITRGLEPVSQIFKQHVTNEGTALVKQAEDAASNKKPEKKEIVGLQ
                     EQVFVRKIIELHDKYVAYVTDCFQGHTLFHKALKEAFEVFCNKGVSGSSSAELLATFCD
                     NILKKGGSEKLSDEAIEDTLEKVVRLLAYISDKDLFAEFYRKKLARRLLFDKSANDEHE
                     RSILTKLKQQCGGQFTSKMEGMVTDLTVARDHQAKFEEFISTHSELNPGIALAVTVLTT
                     GFWPSYKSFDINLPAEMVKCVEVFKEFYQTRTKHRKLTWIYSLGTCNINAKFEAKTIEL
                     IVTTYQAALLLLFNGVDRLSYSEIVTQLNLSDDDVVRLLHSLSCAKYKILSKEPNNRSI
                     SPNDVFEFNSKFTDKLRRLKIPLPPVDEKKKVVEDVDKDRRYAIDASIVRIMKSRKVLG
                     HQQLVMECVEQLGRMFKPDFKAIKKRIEDLITRDYLERDKDNPNVYRYLAGSGSSEVHT
                     NQDPLD"
     misc_feature    2691..3097
                     /label=PFR1 inter ORF
     CDS             3098..3664
                     /codon_start=1
                     /label=Os SKP1
                     /translation="MAAEGEKKMITLKSSDGEEFEVEEAVAMESQTIRHMIEDDCADNG
                     IPLPNVNSKILSKVIEYCNKHVHAAAAAASKAADDAASAAAAVPPPSGEDLKNWDADFV
                     KVDQATLFDLILAANYLNIKGLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEE
                     IRRENQWAFEGSGSSEVHTNQDPLD"
     misc_feature    3665..4064
                     /label=PFR1 inter ORF
     CDS             4065..4454
                     /codon_start=1
                     /label=Os RBX1
                     /translation="MDKGDVAVAVPPSIAGASSSGAKKGKRFEIKKWNAVSLWAWDIVV
                     DNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDN
                     SEWEFQKYGHGSGSSEVHTNQDPLD"
     misc_feature    4455..4849
                     /label=PFR1 inter ORF
     CDS             4851..5647
                     /codon_start=1
                     /label=G418 R
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    5649..6292
                     /label=beta to alpha tubulin inter ORF
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggccgcggga ttttaattaa
       61 tagaaagtgt gacaacgtcg caccatgtgt aggttttcat ttatgttctt tctttctttt
      121 ttttgtgaat ttgttttctg tctcaaatgt ttttaattcg cttgggacct atgtttttct
      181 tgtttttttg ctcacccttt gtgtaggagg caccctgtca cgtctgtggt tgcgtgtatg
      241 ccttccttcc ccttattcgc ttcttcctgt cgtgtcacac ctctttctct ctctcccttt
      301 ccgccttttc tttcaatctt gttttctcga ccagccctac tagaggagaa agaatagtaa
      361 ccctttcatc aaagaaaata gttcaaacga attcataagc ttccgcaaca atggcaacgc
      421 acgagcgcaa gacgattgac ctcgagcaag gatgggagtt catgcaaaag ggaattacga
      481 agctcaagaa cattctcgag ggaaagcccg agccccaatt cagcagcgag gactacatga
      541 tgctctacac gacgatttac aacatgtgca cgcaaaagcc cccccacgac tacagccaac
      601 aactctacga gaagtaccgc gagagcttcg aggagtacat tacgagcatg gtgctcccca
      661 gcctccgcga gaagcacgac gagttcatgc tccgcgagct cgtgaagcgc tggagcaacc
      721 acaaggtgat ggtgcgctgg ctcagccgct tcttccacta cctcgaccgc tacttcatta
      781 gccgccgcag cctcccccaa ctcagcgagg tgggactcag ctgcttccgc gacctcgtgt
      841 accaagagat taagggaaag gtgaagagcg cagtgattag cctcattgac caagagcgcg
      901 agggagagca aattgaccgc gcactcctca agaacgtgct cgacattttc gtggagattg
      961 gactcacgag catggactac tacgagaacg acttcgagga cttcctcctc aaggacacgg
     1021 cagactacta cagcattaag gcacaaacgt ggattctcga ggacagctgc cccgactaca
     1081 tgctcaaggc agaggagtgc ctcaagcgcg agaaggagcg cgtggcacac tacctccaca
     1141 gcagcagcga gcaaaagctc ctcgagaagg tgcaacacga gctcctcacg caatacgcaa
     1201 gccaactcct cgagaaggag cacagcggat gccacgcact cctccgcgac gacaaggtgg
     1261 acgacctcag ccgcatgtac cgcctcttca gccgcattac gcgcggactc gagcccgtga
     1321 gccaaatttt caagcaacac gtgacgaacg agggaacggc actcgtgaag caagcagagg
     1381 acgcagcaag caacaagaag cccgagaaga aggagattgt gggactccaa gagcaagtgt
     1441 tcgtgcgcaa gattattgag ctccacgaca agtacgtggc atacgtgacg gactgcttcc
     1501 aaggacacac gctcttccac aaggcactca aggaggcatt cgaggtgttc tgcaacaagg
     1561 gagtgagcgg aagcagcagc gcagagctcc tcgcaacgtt ctgcgacaac attctcaaga
     1621 agggaggaag cgagaagctc agcgacgagg caattgagga cacgctcgag aaggtggtgc
     1681 gcctcctcgc atacattagc gacaaggacc tcttcgcaga gttctaccgc aagaagctcg
     1741 cacgccgcct cctcttcgac aagagcgcaa acgacgagca cgagcgcagc attctcacga
     1801 agctcaagca acaatgcgga ggacaattca cgagcaagat ggagggaatg gtgacggacc
     1861 tcacggtggc acgcgaccac caagcaaagt tcgaggagtt cattagcacg cacagcgagc
     1921 tcaaccccgg aattgcactc gcagtgacgg tgctcacgac gggattctgg cccagctaca
     1981 agagcttcga cattaacctc cccgcagaga tggtgaagtg cgtggaggtg ttcaaggagt
     2041 tctaccaaac gcgcacgaag caccgcaagc tcacgtggat ttacagcctc ggaacgtgca
     2101 acattaacgc aaagttcgag gcaaagacga ttgagctcat tgtgacgacg taccaagcag
     2161 cactcctcct cctcttcaac ggagtggacc gcctcagcta cagcgagatt gtgacgcaac
     2221 tcaacctcag cgacgacgac gtggtgcgcc tcctccacag cctcagctgc gcaaagtaca
     2281 agattctcag caaggagccc aacaaccgca gcattagccc caacgacgtg ttcgagttca
     2341 acagcaagtt cacggacaag ctccgccgcc tcaagattcc cctccccccc gtggacgaga
     2401 agaagaaggt ggtggaggac gtggacaagg accgccgcta cgcaattgac gcaagcattg
     2461 tgcgcattat gaagagccgc aaggtgctcg gacaccaaca actcgtgatg gagtgcgtgg
     2521 agcaactcgg acgcatgttc aagcccgact tcaaggcaat taagaagcgc attgaggacc
     2581 tcattacgcg cgactacctc gagcgcgaca aggacaaccc caacgtgtac cgctacctcg
     2641 caggttccgg atcttccgag gtccacacca accaggaccc gctcgactag tgtggccgca
     2701 attattatgt aaattgagtg caactagtta tttgatgcca atgtcttgtg cagtcttttc
     2761 ttattcttgt actagatgta ataaaggccc aactgggtcg gtatatgtag gtcaccatga
     2821 tgcgttttcg agcatccgct ctgttattgt gccaaaccga cggcttccgg caccaccact
     2881 tgcctgtgta gctttaacat tgcactgatg ctcacacttt tcttcatcat cttgtacgcg
     2941 tccggcaaac agtttttcgc aatagcttgt aggaaagcaa ggaataagat ctagaaacat
     3001 cacgtaagaa cttcaagcac cagacagaaa tcacacaact ttcaacacaa gaaatcgatt
     3061 atccttaata aaagagtgct accggatccc cgcaacaatg gctgccgagg gcgagaaaaa
     3121 gatgattacg ctcaagagct ccgacggtga ggagttcgag gtggaggagg ccgtcgctat
     3181 ggagagccag accatccgcc acatgattga ggacgactgc gcagacaacg gaatccccct
     3241 ccccaacgtc aactccaaga ttctcagcaa ggtgatcgag tattgcaaca agcacgtcca
     3301 cgctgccgcc gctgccgcat ccaaagcagc cgacgacgct gcttccgccg cagccgccgt
     3361 gcccccgccg agcggcgagg acctcaagaa ctgggacgct gacttcgtca aggtcgacca
     3421 ggctacactc ttcgacctca tcctcgctgc aaactatctc aacattaaag gactcctcga
     3481 cctcacgtgc cagacggtgg cagacatgat caagggaaaa acaccggagg agattcgtaa
     3541 gacgttcaac attaagaacg acttcacacc ggaggaggag gaggagatcc gtcgtgagaa
     3601 ccagtgggca ttcgagggtt ccggatcttc cgaggtccac accaaccagg acccgctcga
     3661 ctagtgtggc cgcaattatt atgtaaattg agtgcaacta gttatttgat gccaatgtct
     3721 tgtgcagtct tttcttattc ttgtactaga tgtaataaag gcccaactgg gtcggtatat
     3781 gtaggtcacc atgatgcgtt ttcgagcatc cgctctgtta ttgtgccaaa ccgacggctt
     3841 ccggcaccac cacttgcctg tgtagcttta acattgcact gatgctcaca cttttcttca
     3901 tcatcttgta cgcgtccggc aaacagtttt tcgcaatagc ttgtaggaaa gcaaggaata
     3961 agatctagaa acatcacgta agaacttcaa gcaccagaca gaaatcacac aactttcaac
     4021 acaagaaatc gattatcctt aataaaagag tgctacccgc aacaatggac aagggagacg
     4081 tggcagtggc agtgcccccc agcattgcag gagcaagcag cagcggagca aagaagggaa
     4141 agcgcttcga gattaagaag tggaacgcag tgagcctctg ggcatgggac attgtggtgg
     4201 acaactgcgc aatttgccgc aaccacatta tggacctctg cattgagtgc caagcaaacc
     4261 aagcaagcgc aacgagcgag gagtgcacgg tggcatgggg agtgtgcaac cacgcattcc
     4321 acttccactg cattagccgc tggctcaaga cgcgccaagt gtgccccctc gacaacagcg
     4381 agtgggagtt ccaaaagtac ggacacggtt ccggatcttc cgaggtccac accaaccagg
     4441 acccgctcga ctagtgtggc cgcaattatt atgtaaattg agtgcaacta gttatttgat
     4501 gccaatgtct tgtgcagtct tttcttattc ttgtactaga tgtaataaag gcccaactgg
     4561 gtcggtatat gtaggtcacc atgatgcgtt ttcgagcatc cgctctgtta ttgtgccaaa
     4621 ccgacggctt ccggcaccac cacttgcctg tgtagcttta acattgcact gatgctcaca
     4681 cttttcttca tcatcttgta cgcgtccggc aaacagtttt tcgcaatagc ttgtaggaaa
     4741 gcaaggaata agatctagaa acatcacgta agaacttcaa gcaccagaca gaaatcacac
     4801 aactttcaac acaagaaatc gattatcctt aataaaagag tgctacccat atgattgaac
     4861 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4921 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4981 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     5041 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5101 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5161 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5221 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5281 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5341 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     5401 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5461 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5521 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5581 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5641 tctgattcga atagacgcgg acggggcatt tcccgttcgt cattagcagt aggtaatgaa
     5701 gatgtttgtt tctcgtcccc tttctccttc gtccttctgt cattttgttc ttttgtgttt
     5761 atgttttgtt gttgttttct ttaatttttt ttttcttcca cgtttgtgta catccgcgcg
     5821 ccactctatt cagagagcca cggatagtag aggaggtggg aagggtatat gagggacacg
     5881 cgtaccatga tgtgggatgt attggggtcc ctgtctgtcc ttacgtgact atgtatgaac
     5941 cgtcacgtgt aagatgagct agtgagatca acagtacaac tcatcaacac gccttcttct
     6001 cgttaaatgt acacaatctt gatcctccac ctttatgggt cccattgttt gcctcttccg
     6061 ctgtgtggag tgcgcctaca cgcacttctc acttcgtaag tggtggtggc gtaagtattg
     6121 cctaatgttg actctatatt ctcctctcct caccccctcg cggtgctgat ttctgacaga
     6181 tcttcaaaca ctagtttaag caaaggacta ttcatccgtt tatattagca acagtaggta
     6241 ctagcaccac taacaacaac aacaaagcac ttctatttat ttatcatgtt aattaaaatc
     6301 actagtgcgg ccgcctgcat agcttgagta ttctatagtg tcacctaaat agcttggcgt
     6361 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6421 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6481 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6541 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6601 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6661 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6721 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6781 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6841 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6901 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6961 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7021 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7081 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7141 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7201 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7261 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7321 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7381 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7441 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7501 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7561 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7621 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7681 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7741 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7801 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7861 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7921 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7981 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8041 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8101 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8161 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8221 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8281 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8341 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8401 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8461 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8521 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     8581 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     8641 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     8701 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     8761 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     8821 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     8881 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     8941 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     9001 cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga
     9061 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     9121 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     9181 cagtgaattg taatacgact cactata
//
