LOCUS       pXYB033_sfGFP_Te       14189 bp ds-DNA     circular     22-JUN-2023
DEFINITION  .
KEYWORDS    "creator:Xiaohan Yang" "marker:NeoR/KanR, KanR, Modified
            COR47-5'-UTR"
COMMENT     35S::sfGFP (original sequence from the publication of A split
            ribozyme that links detection of a native RNA to orthogonal protein
            outputs)
FEATURES             Location/Qualifiers
     promoter        complement(16..361)
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     primer_bind     738..754
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     promoter        1269..1614
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     misc_feature    1599..1623
                     /label="overlap1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1624..1730
                     /label="Modified COR47-5'-UTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1706..1735
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1706..3002
                     /label="gYL054"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1739..2455
                     /label="sfGFP"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1739..2455
                     /label="sfGFP"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK*"
     misc_feature    2456..3333
                     /label="HSPT878"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2481..2505
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2967..3002
                     /label="gibson overhang"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3334..3354
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3385..3409
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(3395..3411)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    3419..3435
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3443..3449)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3450..3467)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3468..3473)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    3488..3509
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        3700..4377
                     /label="CaMV 35S promoter (enhanced)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: cauliflower mosaic virus 35S promoter with a duplicated enhancer region"
     misc_feature    4381..5307
                     /label="gBlocks for change to Kan"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4445..5242
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-II (or nptII) note: confers resistance to neomycin, kanamycin, and G418 (Geneticin®) product: aminoglycoside phosphotransferase from Tn5 transl_table: 1 translation: MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     polyA_signal    5282..5456
                     /label="CaMV poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: cauliflower mosaic virus polyadenylation signal"
     misc_feature    5534..5558
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: left border repeat from nopaline C58 T-DNA"
     CDS             5983..6777
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aphA-3 note: confers resistance to kanamycin product: aminoglycoside phosphotransferase protein_id: transl_table: 1 translation: MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"
     rep_origin      6864..7452
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     misc_feature    7638..7778
                     /label="bom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: basis of mobility region from pBR322"
     rep_origin      8122..8316
                     /label="pVS1 oriV"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)"
     CDS             complement(8382..9449)
                     /label="pVS1 RepA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 note: product: replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) protein_id: transl_table: 1 translation: GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     CDS             complement(9883..10512)
                     /label="pVS1 StaA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 product: stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) transl_table: 1 translation: MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
     misc_feature    11812..11836
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: right border repeat from nopaline C58 T-DNA"
     terminator      11858..12110
                     /label="NOS terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: nopaline synthase terminator and poly(A) signal"
     CDS             complement(12142..12159)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: 6xHis affinity tag transl_table: 1 translation: HHHHHH"
     CDS             complement(12166..13983)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          13984..14173
                     /label="cat1 intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: castor bean catalase intron, modified"
     CDS             complement(13984..14173)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     CDS             complement(14174..14188)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actCAAACAT TACTCATTCA CAAAACCATC TTAAAGCAAC TACACAAGTC TTGAAATTTT
     1681 CTCATATTTT CTATTTACTA TATAAACTTT TAATCAAATC AAGATTAAAG TTAATTAAAT
     1741 GAGCAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA
     1801 TGTTAATGGG CACAAATTTT CTGTCCGTGG AGAGGGTGAA GGTGATGCTA CAAACGGAAA
     1861 ACTCACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCGTGGC CAACACTTGT
     1921 CACTACTCTG ACCTATGGTG TTCAATGCTT TTCCCGTTAT CCGGATCACA TGAAACGGCA
     1981 TGACTTTTTC AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAACGCACTA TATCTTTCAA
     2041 AGATGACGGG ACCTACAAGA CGCGTGCTGA AGTCAAGTTT GAAGGTGATA CCCTTGTTAA
     2101 TCGTATCGAG TTAAAGGGTA TTGATTTTAA AGAAGATGGA AACATTCTTG GACACAAACT
     2161 CGAGTACAAC TTTAACTCAC ACAATGTATA CATCACGGCA GACAAACAAA AGAATGGAAT
     2221 CAAAGCTAAC TTCAAAATTC GCCACAACGT TGAAGATGGT TCCGTTCAAC TAGCAGACCA
     2281 TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT
     2341 GTCGACACAA TCTGTCCTTT CGAAAGATCC CAACGAAAAG CGTGACCACA TGGTCCTTCT
     2401 TGAGTTTGTA ACTGCTGCTG GGATTACACA TGGCATGGAT GAGCTCTACA AATAAATATG
     2461 AAGATGAAGA TGAAATATTT GGTGTGTCAA ATAAAAAGCT TGTGTGCTTA AGTTTGTGTT
     2521 TTTTTCTTGG CTTGTTGTGT TATGAATTTG TGGCTTTTTC TAATATTAAA TGAATGTAAG
     2581 ATCTCATTAT AATGAATAAA CAAATGTTTC TATAATCCAT TGTGAATGTT TTGTTGGATC
     2641 TCTTCTGCAG CATATAACTA CTGTATGTGC TATGGTATGG ACTATGGAAT ATGATTAAAG
     2701 ATAAGATGGG CTCATAGAGT AAAACGAGGC GAGGGACCTA TAAACCTCCC TTCATCATGC
     2761 TATTTCATGA TCTATTTTAT AAAATAAAGA TGTAGAAAAA AGTAAGCGTA ATAACCGCAA
     2821 AACAAATGAT TTAAAACATG GCACATAATG AGGAGATTAA GTTCGGTTTA CGTTTATTTT
     2881 AGTACTAATT GTAACGTGAG ACTACGTATC GGGAATCGCC TAATTAAAGC ATTAATGCGA
     2941 ACCTGATTAG ATTCACCGAC CCTCCTATCG TGTCGACCTT TCTGTTTCTT AGAATTTTTT
     3001 GGTAGTCTAT GTACTAATAA TGTCAGCTTC GTATTTATTT CATAAGCAAT TTGCATTTGC
     3061 AATTTGTTTT TTACTTTTAT TTTTATTGTA TTGTGGAATG TGGACTCGTA CCAACATGAA
     3121 GTTATATACC ACCAAAAAAA TTACAGTTAG TCAAAAGATT CACGAGTGAG AGCTACTTAT
     3181 GATTGTCTTT TACGTATATG TCTAATTGTC TATTTGCTCA ATAATCTTTG TACTTTCTTT
     3241 TGTCGTTGAT AAAATCACAA AGTTCCAAAA GTAATCGAAT GATTTGCTTT TAAGAAAAGA
     3301 AGAGCTCAAT AATTCAACAT ATATCTGTAC ACAattaccg cctttgagtg agctctagat
     3361 gccacctgac gtctaagaag aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     3421 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3481 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3541 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3601 tgcgtattgg ctagagcagc ttgccaacat ggtggagcac gacactctcg tctactccaa
     3661 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
     3721 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
     3781 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
     3841 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     3901 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg ataacatggt
     3961 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     4021 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     4081 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     4141 tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga
     4201 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     4261 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     4321 ttcgcaagac cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaaat
     4381 caccagtctc tctctacaaa tctatctctc tcgagctttc gcagatcccg gggggcaatg
     4441 agatatgggg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4501 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4561 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4621 tgaactccag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4681 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4741 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4801 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4861 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4921 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     4981 gcccgacggc gaggatctcg tcgtgacaca tggcgatgcc tgcttgccga atatcatggt
     5041 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5101 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5161 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5221 ccttcttgac gagttcttct gaagtagatg ccgaccggat ctgtcgatcg acaagctcga
     5281 gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt cctatagggt
     5341 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     5401 tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatccccc
     5461 gaattaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc
     5521 taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg
     5581 accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg
     5641 tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa
     5701 gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg
     5761 attgtaacga tgacagagcg ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt
     5821 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
     5881 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg
     5941 gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata
     6001 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
     6061 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
     6121 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
     6181 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
     6241 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
     6301 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
     6361 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
     6421 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
     6481 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
     6541 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
     6601 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
     6661 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
     6721 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
     6781 ctagaatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6841 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6901 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6961 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     7021 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7081 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7141 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7201 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7261 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7321 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7381 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7441 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7501 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7561 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7621 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     7681 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     7741 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     7801 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7861 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
     7921 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
     7981 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
     8041 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
     8101 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
     8161 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
     8221 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
     8281 tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt
     8341 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
     8401 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
     8461 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
     8521 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
     8581 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
     8641 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
     8701 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
     8761 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
     8821 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
     8881 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
     8941 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
     9001 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
     9061 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
     9121 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
     9181 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
     9241 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
     9301 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
     9361 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
     9421 atttgtaccg ggccggatgg tttgcgaccg tcacgccgat tcctcgggct tgggggttcc
     9481 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
     9541 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
     9601 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
     9661 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
     9721 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
     9781 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
     9841 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
     9901 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
     9961 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
    10021 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    10081 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
    10141 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
    10201 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
    10261 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
    10321 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
    10381 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
    10441 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
    10501 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
    10561 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
    10621 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
    10681 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
    10741 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    10801 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    10861 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    10921 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    10981 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    11041 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    11101 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    11161 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    11221 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    11281 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    11341 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    11401 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    11461 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    11521 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    11581 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    11641 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    11701 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt
    11761 ttcacgccct tttaaatatc cgttattcta ataaacgctc ttttctctta ggtttacccg
    11821 ccaatatatc ctgtcaaaca ctgatagttt aattcccgat ctagtaacat agatgacacc
    11881 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
    11941 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
    12001 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
    12061 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc ggggaaattc
    12121 gagctggtca ccaattcaca cgtgatggtg atggtgatgg ctagcgttct tgtagccgaa
    12181 atctggaatg ttggtccagc gctcgcgaaa gacgtgcgcg gcgagcttcg gcttgcggtc
    12241 acgagtgaac acgcccttct tgtttccttg gacgcgcatc acgccctgag aggtcgcgaa
    12301 gtccgcgaag ttccacgctt gctcacccac gaagttctca aactcatcga acacgacgtg
    12361 gttcgcctgg tagtactcga cttgatattc ctcggtgaac atcactggat caatgtcgtg
    12421 aaagcccgca acggtgtctg cgccgtactc agtgatcatg atcggctttc ctgggcaacg
    12481 cttgttccac gcgtgaaatt cctggcggag atggactttg gccgcttcga gatcaccgcc
    12541 atcgaagtac catccgttat agcgattgag cgcgatgacg tcaatcagtt cggcgacttt
    12601 gtccgtctcc ggggtagcca tcacaaacag cacgatcgtg accggacgct tctgtgggtc
    12661 gagttccttg gtcagctcca ccaacggctt gaagtactcg tacgcgccct cttcctcagt
    12721 cgccgcctcg ttggcgatgc tccacatcac gacgcttgga tggttcttgt cacgagacac
    12781 cagttcacgg agaacgtctt gatggtgctc aaacgtccga atcttctccc aggtactgac
    12841 gcgctcgctg ccttcgccga gtcccgtggt ggccatgaag ttgaggtgca cgccaactgc
    12901 cggagtctcg tcgatcacga ccagaccctc gcgatccgca agacgcatca actcttcaga
    12961 gtacggatag tgtgcggtcc ggaagctgtt ggcgccgatc catttgagga tattgaaatc
    13021 catcacattg ctcgcttcgt taaagccacg gccgttgata ggagtgtcct catgtttgcc
    13081 aaagcccttg aagtagaacg gtttgttgtt gatgaggaac ttgccgtcgt tgacttccac
    13141 ggtccgcacg ccgaacggct cttcatagac atcgatggtc agtccgtcgt tcaccagttc
    13201 cactttgatc tggtagagat acgtgttcag tggttcccag aggatgacat tcggaatctc
    13261 cacgttaccg ctcaggccct cggtgcttgc gaccactttg ccttcctcat ccacgaccga
    13321 cactttcacg gtctcggctt tgccttgaaa gtccaccgta taggtcacag tcccggttgg
    13381 gccattgaag tcggtcacaa ccgagatgtc ctcgacgtac gtaaacgggg tcgtgtagat
    13441 tttcaccgga cggtgcaggc ctgcatagtt gaagaagtcg aagttcggct tgttacgaat
    13501 gacttttccg aggccctctt cgtggcgctc gctgtacagc cccaccggga gggtgctatc
    13561 gtcgaggatg ttgtccacgg cgacggtgac gcgattcatg ccatcacgca gcgagttgtt
    13621 gatttccgct tcgaatggca ggaatccgcc cttgtgctcc acgaccagct caccattgac
    13681 atagacaatt gctttgtgag ttgcagagcc gaagcggagc acgatacgct gatccttcag
    13741 ataggccggc accgtgaact cacgttcgta ccagacatat ccgatatggt tgcggatttc
    13801 cttggtcacg ccaatgtcat tgtaactgct tgggacggcc atactaatag tgtcggtcag
    13861 cttgctttcg taccacttct cttccagtcc tttcccgtag tccagcttga agttccagac
    13921 gccattgagg tcgaagacgc cacgggtctc ggtgttgatc gggtacagac tagttcgtcg
    13981 gttctgtaac tatcatcatc atcatagaca cacgaaataa agtaatcaga ttatcagtta
    14041 aagctatgta atatttacac cataaccaat caattaaaaa atagatcagt ttaaagaaag
    14101 atcaaagctc aaaaaaataa aaagagaaaa gggtcctaac caagaaaatg aaggagaaaa
    14161 actagaaatt taccctcaga tctaccatg
//