LOCUS       Exported                4710 bp ds-DNA     circular SYN 30-DEC-2024
DEFINITION  TSN20241016-0571-00013_764_20241017-BAN40_A01_A01_i14.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 29, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      24..159
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             208..1002
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    1234..1281
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1610..2198
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        2373..2676
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2677..2880
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2932..3018
                     /codon_start=1
                     /label=cytochrome p450
                     /translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGKL"
     regulatory      3064..3073
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3070..3759
                     /codon_start=1
                     /label=mScarlet3-H
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     polyA_signal    3882..4003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4010..4465)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4492..4596
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(4665..4710,1..165)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        join(4687..4710,1..173)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
ORIGIN
        1 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
       61 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
      121 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
      181 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
      241 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
      301 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
      361 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
      421 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
      481 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
      541 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
      601 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
      661 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
      721 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
      781 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
      841 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
      901 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
      961 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     1021 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     1081 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     1141 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
     1201 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     1261 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     1321 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     1381 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     1441 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1501 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1561 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1621 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1681 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1741 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1801 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1861 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1921 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1981 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2041 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2101 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2161 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2221 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2281 attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg
     2341 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     2401 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     2461 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     2521 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     2581 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     2641 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     2701 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     2761 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     2821 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     2881 ggtttagtga accgtcagat ccgctagcgc taccggactc agatctccac catggatcct
     2941 gtggtggtgc tggggctctg tctctcctgt ttgcttctcc tttcactctg gaaacagagc
     3001 tatgggggag ggaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg
     3061 gtcgccacca tggatagcac cgaggcagtg atcaaggagt tcatgcggtt caaggtgcac
     3121 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     3181 tacgagggca cccagaccgc caagctgagg gtgaccaagg gtggccccct gcccttctcc
     3241 tgggacatcc tgtcccctca gttcatgtac ggctccaggg ccttcacgaa gcaccccgcc
     3301 gacatccccg actactggaa gcagtccttc cccgagggct tcaagtggga gcgcgtgatg
     3361 aacttcgagg acggcggcgc cgtgtccgtg gcccaggaca cctccctgga ggacggcacc
     3421 ctgatctaca aggtgaagct ccgcggcacc aacttccctc ctgacggccc cgtaatgcag
     3481 aagaagacaa tgggctggga agcatccacc gagcggttgt accccgagga cgtcgtgctg
     3541 aagggcgaca ttaagcatgc cctgcgcctg aaggacggcg gccggtacct ggcggacttc
     3601 aagaccacct acagggccaa gaagcccgtg cagatgcccg gcgccttcaa catcgaccgc
     3661 aagttggaca tcacatccca caacgaggac tacaccgtgg tggaacagta cgaacgctcc
     3721 gtggcccgcc actccaccgg cggctccggt ggctcctaaa gcggccgcga ctctagatca
     3781 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     3841 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     3901 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3961 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc
     4021 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     4081 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     4141 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     4201 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     4261 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     4321 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     4381 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     4441 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4501 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4561 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt
     4621 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4681 aaagcatgca tctcaattag tcagcaacca
//
