LOCUS       pMDS2_Addgene           8892 bp    DNA        circular     08-MAY-2025
DEFINITION  synthetic DNA
ACCESSION   .
VERSION     .
KEYWORDS    NEBuilder
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   .
  TITLE     NEBuilder-generated Construct
  JOURNAL   Exported  23-MAY-2021 from NEBuilder https://nebuilder.neb.com
COMMENT     NEBuilder-generated oligos (UPPERCASE = gene-specific, lowercase =
            overlap)
COMMENT     3HA_fwd: gcggtatcgctcttcatggaTGGCATTAACATCTTTTACC
COMMENT     3HA_fwd 3'Tm: 57.0  3'Ta: 56.2
COMMENT     3HA_rev: gtgatttcagcgtaccgacaTTACTCTTCTTCTTGATCAG
COMMENT     3HA_rev 3'Tm: 55.2  3'Ta: 56.2
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(6346..6362)
                     /note="In lacZ gene. Also called M13-rev"
                     /locus_tag="M13 Reverse"
                     /label="M13 Reverse"
                     /ApEinfo_label="M13 Reverse"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6577..6594)
                     /note="L4440 vector, forward primer"
                     /locus_tag="L4440"
                     /label="L4440"
                     /ApEinfo_label="L4440"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             7614..8324
                     /locus_tag="mTurquoise2"
                     /label="mTurquoise2"
                     /ApEinfo_label="mTurquoise2"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     6259..6276
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21)
                     Forward"
                     /locus_tag="M13 Forward"
                     /label="M13 Forward"
                     /ApEinfo_label="M13 Forward"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6750..6779
                     /locus_tag="G4S4linker"
                     /label="G4S4linker"
                     /ApEinfo_label="G4S4linker"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     6657..6749
                     /locus_tag="3XHA"
                     /label="3XHA"
                     /ApEinfo_label="3XHA"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    77..101
                     /note="right border repeat from nopaline C58 T-DNA"
                     /locus_tag="RB T-DNA repeat"
                     /label="RB T-DNA repeat"
                     /ApEinfo_label="RB T-DNA repeat"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      2711..3146
                     /note="origin of replication from bacterial plasmid pSa"
                     /locus_tag="pSa ori"
                     /label="pSa ori"
                     /ApEinfo_label="pSa ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            6780..7499
                     /note="VENUS"
                     /locus_tag="VENUS"
                     /label="VENUS"
                     /ApEinfo_label="VENUS"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      5677..5929
                     /note="nopaline synthase terminator and poly(A) signal"
                     /locus_tag="NOS terminator"
                     /label="NOS terminator"
                     /ApEinfo_label="NOS terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7548..7607
                     /vntifkey="21"
                     /locus_tag="Peptide\2A"
                     /label="Peptide\2A"
                     /ApEinfo_label="Peptide\2A"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     7573..7590
                     /locus_tag="mTURQ_F_education"
                     /label="mTURQ_F_education"
                     /ApEinfo_label="mTURQ_F_education"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    6370..6386
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /locus_tag="lac operator"
                     /label="lac operator"
                     /ApEinfo_label="lac operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(272..291)
                     /note="pBR322 origin, forward primer"
                     /locus_tag="pBR322ori-F"
                     /label="pBR322ori-F"
                     /ApEinfo_label="pBR322ori-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             8325..8579
                     /vntifkey="4"
                     /note="Coding"
                     /locus_tag="N7"
                     /label="N7"
                     /ApEinfo_label="N7"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(6027..6350)
                     /codon_start="1"
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTI
                     CSDAA"
                     /locus_tag="lacZ-alpha"
                     /label="lacZ-alpha"
                     /ApEinfo_label="lacZ-alpha"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     8078..8097
                     /locus_tag="possible primer"
                     /label="possible primer"
                     /ApEinfo_label="possible primer"
                     /ApEinfo_fwdcolor="#fc7f08"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(6394..6424)
                     /note="promoter for the E. coli lac operon"
                     /locus_tag="lac promoter"
                     /label="lac promoter"
                     /ApEinfo_label="lac promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     6032..6051
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
                     /locus_tag="pRS-marker"
                     /label="pRS-marker"
                     /ApEinfo_label="pRS-marker"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    6439..6460
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /locus_tag="CAP binding site"
                     /label="CAP binding site"
                     /ApEinfo_label="CAP binding site"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(871..1659)
                     /codon_start="1"
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /locus_tag="SmR"
                     /label="SmR"
                     /ApEinfo_label="SmR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(192..780)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3274..3296
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
                     /locus_tag="LB T-DNA repeat"
                     /label="LB T-DNA repeat"
                     /ApEinfo_label="LB T-DNA repeat"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        3695..4040
                     /note="strong constitutive promoter from cauliflower
                     mosaic virus"
                     /locus_tag="CaMV 35S promoter"
                     /label="CaMV 35S promoter"
                     /ApEinfo_label="CaMV 35S promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4948..5658
                     /codon_start="1"
                     /product="monomeric derivative of DsRed fluorescent
                     protein (Shaner et al., 2004)"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMEELYR"
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    8632..8808
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /locus_tag="CaMV poly(A) signal"
                     /label="CaMV poly(A) signal"
                     /ApEinfo_label="CaMV poly(A) signal"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(8872..8892)
                     /note="T3 promoter, forward primer"
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(8872..8890)
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /locus_tag="T3 promoter"
                     /label="T3 promoter"
                     /ApEinfo_label="T3 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7533..7537
                     /locus_tag="G4S4linker(1)"
                     /label="G4S4linker(1)"
                     /ApEinfo_label="G4S4linker"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7500..7532
                     /locus_tag="G4S4linker(2)"
                     /label="G4S4linker(2)"
                     /ApEinfo_label="G4S4linker"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gcgctagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
       61 aagaacatga aggccttgac aggatatatt ggcgggtaaa ctaagtcgct gtatgtgttt
      121 gtttgagatc tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      181 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      241 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      301 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      361 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      421 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      481 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      541 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      601 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      661 aagccagtta ccttcggaag aagagttggt agctcttgat ccggcaaaca aaccaccgct
      721 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
      781 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
      841 gggattttgg tcatgaattc ttgttagaca ttatttgccg actaccttgg tgatctcgcc
      901 tttcacgtag tgaacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttg
      961 tccaagataa gcctgcctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
     1021 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
     1081 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
     1141 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
     1201 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
     1261 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
     1321 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
     1381 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
     1441 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
     1501 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
     1561 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
     1621 tagttgagtc gatacttcgg cgatcaccgc ttccctcatg atgtttaact cctgaattaa
     1681 gccgcgccgc gaagcggtgt ggcttgaatg aattgttagg cgtcatcctg tgctcccgag
     1741 aaccagtacc agtacatcgc tgtttcgttc gagacttgag gtctagtttt atacgtgaac
     1801 aggtcaatgc cgccgagagt aaagccacat ttgcgtacaa attgcaggca ggtacattgt
     1861 tcgtttgtgt ctctaatcgt atgccaagga gctgtctgct tagtgcccac tttttcgcaa
     1921 aattcgatga gactgtgcgc gactcctttg cctcggtgcg tgtgcgacac aacaatgtgt
     1981 tcgatagagg ctagatcgtt cccatgttga gttgagttca atcttcccga caagctcttg
     2041 gtcgatgaat gtgccatagc aagcagagtc ttcatcagag tcatcatccg agatgtaatc
     2101 cttccggtag gggctcacac ttctggtaga tagttcaaag ccttggtcgg ataggtgcac
     2161 atcgaacact tcacgaacaa tgaaatggtt ctcagcatcc aatgtttccg ccacctgctc
     2221 agggatcacc gaaatcttca tatgacgcct aacgcctggc acagcggatc gcaaacctgg
     2281 cgcggctttt ggcacaaaag gcgtgacagg tttgcgaatc cgttgctgcc acttgtttaa
     2341 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2401 gctggtttat tgctgataaa tctggagccg gtgagcgtgg atctcgcggt atcattgcag
     2461 cactgccatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     2521 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt
     2581 cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc
     2641 tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt
     2701 caggcgatcc ccatccaaca gcccgccgtc gagcgggctt ttttatcccc ggaagcctgt
     2761 ggatagaggg tagttatcca cgtgaaaccg ctaatgcccc gcaaagcctt gattcacggg
     2821 gctttccggc ccgctccaaa aactatccac gtgaaatcgc taatcagggt acgtgaaatc
     2881 gctaatcgga gtacgtgaaa tcgctaataa ggtcacgtga aatcgctaat caaaaaggca
     2941 cgtgagaacg ctaatagccc tttcagatca acagcttgca aacacccctc gctccggcaa
     3001 gtagttacag caagtagtat gttcaattag cttttcaatt atgaatatat atatcaatta
     3061 ttggtcgccc ttggcttgtg gacaatgcgc tacgcgcacc ggctccgccc gtggacaacc
     3121 gcaagcggtt gcccaccgtc gagcgccttt gcccacaacc cggcggccgg ccgcaacaga
     3181 tcgttttata aatttttttt tttgaaaaag aaaaagcccg aaaggcggca acctctcggg
     3241 cttctggatt tccgatcccc ggaattagac tcttggcagg atatattgtg gtgtaacgtt
     3301 ggcgcgccta ctccaaaaat gtcaaagata cagtctcaga agaccaaagg gctattgaga
     3361 cttttcaaca aagggtaatt tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     3421 acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat cattgcgata
     3481 aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     3541 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     3601 gatgtgacat ctccactgac gtaagggatg acgcacaatc ccacccctac tccaaaaatg
     3661 tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaattt
     3721 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
     3781 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag
     3841 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     3901 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgacatc tccactgacg
     3961 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     4021 catttcattt ggagaggaca gcccagatca actagtctta agtccggagc tagctctaga
     4081 atggcaaagg atgtggaagc cgttcccgga gaaggatttc agacaagaga ctatcaagat
     4141 ccgccaccag ctccgtttat tgatggagcg gagctaaaga agtggtcttt ctacagagca
     4201 gttatcgcag agttcgtagc cactctcctc ttcttataca tcaccgtttt gacagtcatc
     4261 ggttacaaga ttcagtccga tactgatgcc ggtggcgtag attgcggcgg agttggaatc
     4321 ctcggtatcg cttgggcctt tggtggtatg atcttcatcc tcgtctactg caccgccggt
     4381 atctctggtg gtcacattaa cccagcggtg acatttgggc tattcttggc acgtaaagtg
     4441 tcgttaccta gggccctatt gtacataatc gctcagtgtt tgggtgcgat ttgtggagtt
     4501 ggttttgtca aagccttcca aagctcttac tacacccgtt acggaggtgg agccaactct
     4561 ctagccgatg gctacagcac agggaccggt ctagccgcag agatcattgg tactttcgtt
     4621 cttgtctaca ccgtcttctc tgccactgac cccaaacgta gtgccagaga ctcccacgtt
     4681 ccggtgttgg cgccacttcc aatcggattt gccgtgttca tggtacattt ggctaccatt
     4741 cccattaccg gaaccggaat taacccggca aggagtttcg gagctgccgt aatctacaac
     4801 aagagcaagc catgggatga ccactggata ttttgggttg gaccattcat tggagctgcg
     4861 atagctgcat tctaccacca attcgttcta agagcttcag gttctaagtc tcttggatca
     4921 ttcagaagtg ctgccaacgt cggatccatg gtgagcaagg gcgaggagga taacatggcc
     4981 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     5041 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     5101 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     5161 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     5221 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     5281 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     5341 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     5401 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     5461 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     5521 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     5581 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     5641 gaggagctgt acaggtaaag cggccgcccg gctgcagatc gttcaaacat ttggcaataa
     5701 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     5761 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     5821 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     5881 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg gggcgcgccc
     5941 tcgagAgtag aagagccgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     6001 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     6061 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     6121 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     6181 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     6241 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcgagctcgg tacccgggga
     6301 tcctctagag tcgacctgca ggcatgcagg cttggcgtaa tcatggtcat agctgtttcc
     6361 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6421 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6481 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6541 gagaggcggt ttgcgtattg ggcgctcAtc cgcttcctcg ctcactgact cgctgcgctc
     6601 ggtcgttcgg ctgcggcgaG cggtatcgct cttcATGgat gggcattaac atcttttacc
     6661 catacgatgt tcctgactat gcgggctatc cctatgacgt cccggactat gcaggatcct
     6721 atccatatga cgttccagat tacgctTCCG GAGGTGGTGG TTCTGGTGGG GGTGGCTCTg
     6781 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     6841 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     6901 agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     6961 tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     7021 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     7081 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     7141 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     7201 tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
     7261 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc
     7321 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     7381 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     7441 tggagttcgt gaccgccgcc gggatcactc tcggcatggA cgagctgtac aagcatgatT
     7501 CCGGAGGTGG TGGTTCTGGT GGGGGTGGCT CctcaggTGG CTCgggccag ctgttgaatt
     7561 ttgaccttct taagcttgcg ggagacgtcg agtccaaccc tgggcccccc gggGTGAGCA
     7621 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
     7681 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
     7741 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
     7801 CCCTGTCCTG GGGCGTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT
     7861 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
     7921 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
     7981 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
     8041 ACAACTACTT TAGCGACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG
     8101 CCAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC GACCACTACC
     8161 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
     8221 CCCAGTCCAA GCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
     8281 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACattgct gcagcggccg
     8341 aattcaagcg tgaGgagcaa gcaaggaaag ctaaggtgaa caatgagaaa aagacggaaa
     8401 tagtgaaacc agagagttgt agcaatgaag gagatgtcaa ggatctgaaa agaaaggact
     8461 ctgaggatgg aaacgagggt gaggaagaag aagcttcttc gaaaccgaaa aagccaaaag
     8521 ttgctctttc tcatcttcag gacattgacg acacagaagc tgatcaagaa gaagagtaat
     8581 gtcggtacgc tgaaatcacc agtctctctc tacaaatcta tctctctcta ttttctccat
     8641 aaataatgtg tgagtagttt cccgataagg gaaattaggg ttcttatagg gtttcgctca
     8701 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     8761 taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatcga tgggctagag
     8821 cggactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg
     8881 agggttaatt gc    
//
