LOCUS       pMDS1_Addgene           8856 bp    DNA        circular     08-MAY-2025
DEFINITION  Binary vector for Arabidopsis transformation with SapI Golden Gate
            sites and mCherry seed selection.
ACCESSION   .
VERSION     .
KEYWORDS    pGoldenGate-MCY2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6956)
  TITLE     A robust family of Golden Gate Agrobacteirum vectors for plant
            synthetic biology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             4305..5015
                     /locus_tag="mTurquoise2"
                     /label="mTurquoise2"
                     /ApEinfo_label="mTurquoise2"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3..25
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
                     /locus_tag="LB T-DNA repeat"
                     /label="LB T-DNA repeat"
                     /ApEinfo_label="LB T-DNA repeat"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             5016..5270
                     /vntifkey="4"
                     /note="Coding"
                     /locus_tag="N7"
                     /label="N7"
                     /ApEinfo_label="N7"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        424..769
                     /note="strong constitutive promoter from cauliflower
                     mosaic virus"
                     /locus_tag="CaMV 35S promoter"
                     /label="CaMV 35S promoter"
                     /ApEinfo_label="CaMV 35S promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1677..2387
                     /codon_start="1"
                     /product="monomeric derivative of DsRed fluorescent
                     protein (Shaner et al., 2004)"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMEELYR"
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4239..4298
                     /vntifkey="21"
                     /locus_tag="Peptide\2A"
                     /label="Peptide\2A"
                     /ApEinfo_label="Peptide\2A"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      2406..2658
                     /note="nopaline synthase terminator and poly(A) signal"
                     /locus_tag="NOS terminator"
                     /label="NOS terminator"
                     /ApEinfo_label="NOS terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(2756..3079)
                     /codon_start="1"
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTI
                     CSDAA"
                     /locus_tag="lacZ-alpha"
                     /label="lacZ-alpha"
                     /ApEinfo_label="lacZ-alpha"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2989..3005
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 fwd"
                     /label="M13 fwd"
                     /ApEinfo_label="M13 fwd"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3075..3091)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 rev"
                     /label="M13 rev"
                     /ApEinfo_label="M13 rev"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    3099..3115
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /locus_tag="lac operator"
                     /label="lac operator"
                     /ApEinfo_label="lac operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(3123..3153)
                     /note="promoter for the E. coli lac operon"
                     /locus_tag="lac promoter"
                     /label="lac promoter"
                     /ApEinfo_label="lac promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    3168..3189
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /locus_tag="CAP binding site"
                     /label="CAP binding site"
                     /ApEinfo_label="CAP binding site"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3306..3323)
                     /note="L4440 vector, forward primer"
                     /locus_tag="L4440"
                     /label="L4440"
                     /ApEinfo_label="L4440"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    5325..5501
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /locus_tag="CaMV poly(A) signal"
                     /label="CaMV poly(A) signal"
                     /ApEinfo_label="CaMV poly(A) signal"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5565..5585)
                     /note="T3 promoter, forward primer"
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(5565..5583)
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /locus_tag="T3 promoter"
                     /label="T3 promoter"
                     /ApEinfo_label="T3 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5662..5686
                     /note="right border repeat from nopaline C58 T-DNA"
                     /locus_tag="RB T-DNA repeat"
                     /label="RB T-DNA repeat"
                     /ApEinfo_label="RB T-DNA repeat"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(5777..6365)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5857..5876)
                     /note="pBR322 origin, forward primer"
                     /locus_tag="pBR322ori-F"
                     /label="pBR322ori-F"
                     /ApEinfo_label="pBR322ori-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(6456..7244)
                     /codon_start="1"
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /locus_tag="SmR"
                     /label="SmR"
                     /ApEinfo_label="SmR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      8296..8731
                     /note="origin of replication from bacterial plasmid pSa"
                     /locus_tag="pSa ori"
                     /label="pSa ori"
                     /ApEinfo_label="pSa ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             810..1671
                     /locus_tag="PIP2A (plasma membbrane marker)"
                     /label="PIP2A (plasma membbrane marker)"
                     /ApEinfo_label="PIP2A (plasma membbrane marker)"
                     /ApEinfo_fwdcolor="#fb02fe"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            3402..4121
                     /note="VENUS"
                     /locus_tag="VENUS"
                     /label="VENUS"
                     /ApEinfo_label="VENUS"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     4137..4229
                     /locus_tag="3XHA"
                     /label="3XHA"
                     /ApEinfo_label="3XHA"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3369..3401
                     /locus_tag="G4S4linker"
                     /label="G4S4linker"
                     /ApEinfo_label="G4S4linker"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cttggcagga tatattgtgg tgtaacgttg gcgcgcctac tccaaaaatg tcaaagatac
       61 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaattt cgggaaacct
      121 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
      181 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atgcctctgc
      241 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      301 tccaaccacg tcttcaaagc aagtggattg atgtgacatc tccactgacg taagggatga
      361 cgcacaatcc cacccctact ccaaaaatgt caaagataca gtctcagaag accaaagggc
      421 tattgagact tttcaacaaa gggtaatttc gggaaacctc ctcggattcc attgcccagc
      481 tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca
      541 ttgcgataaa ggaaaggcta tcattcaaga tgcctctgcc gacagtggtc ccaaagatgg
      601 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      661 agtggattga tgtgacatct ccactgacgt aagggatgac gcacaatccc actatccttc
      721 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacag cccagatcaa
      781 ctagtcttaa gtccggagct agctctagaa tggcaaagga tgtggaagcc gttcccggag
      841 aaggatttca gacaagagac tatcaagatc cgccaccagc tccgtttatt gatggagcgg
      901 agctaaagaa gtggtctttc tacagagcag ttatcgcaga gttcgtagcc actctcctct
      961 tcttatacat caccgttttg acagtcatcg gttacaagat tcagtccgat actgatgccg
     1021 gtggcgtaga ttgcggcgga gttggaatcc tcggtatcgc ttgggccttt ggtggtatga
     1081 tcttcatcct cgtctactgc accgccggta tctctggtgg tcacattaac ccagcggtga
     1141 catttgggct attcttggca cgtaaagtgt cgttacctag ggccctattg tacataatcg
     1201 ctcagtgttt gggtgcgatt tgtggagttg gttttgtcaa agccttccaa agctcttact
     1261 acacccgtta cggaggtgga gccaactctc tagccgatgg ctacagcaca gggaccggtc
     1321 tagccgcaga gatcattggt actttcgttc ttgtctacac cgtcttctct gccactgacc
     1381 ccaaacgtag tgccagagac tcccacgttc cggtgttggc gccacttcca atcggatttg
     1441 ccgtgttcat ggtacatttg gctaccattc ccattaccgg aaccggaatt aacccggcaa
     1501 ggagtttcgg agctgccgta atctacaaca agagcaagcc atgggatgac cactggatat
     1561 tttgggttgg accattcatt ggagctgcga tagctgcatt ctaccaccaa ttcgttctaa
     1621 gagcttcagg ttctaagtct cttggatcat tcagaagtgc tgccaacgtc ggatccatgg
     1681 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     1741 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     1801 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     1861 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     1921 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     1981 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     2041 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     2101 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     2161 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     2221 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     2281 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     2341 ccgagggccg ccactccacc ggcggcatgg aggagctgta caggtaaagc ggccgcccgg
     2401 ctgcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     2461 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     2521 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     2581 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     2641 catctatgtt actagatcgg ggcgcgccct cgagagtaga agagccggat gccgggagca
     2701 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     2761 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     2821 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     2881 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     2941 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     3001 ccagtgaatt cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaggc
     3061 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3121 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3181 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3241 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3301 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcgctc
     3361 ttcatggaTC CGGAGGTGGT GGTTCTGGTG GGGGTGGCTC Tgtgagcaag ggcgaggagc
     3421 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3481 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
     3541 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
     3601 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3661 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     3721 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3781 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     3841 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     3901 tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc
     3961 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc
     4021 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     4081 ccgggatcac tctcggcatg gacgagctgt acaagcatga tggcattaac atcttttacc
     4141 catacgatgt tcctgactat gcgggctatc cctatgacgt cccggactat gcaggatcct
     4201 atccatatga cgttccagat tacgctTCCG GCTCgggcca gctgttgaat tttgaccttc
     4261 ttaagcttgc gggagacgtc gagtccaacc ctgggccccc cgggGTGAGC AAGGGCGAGG
     4321 AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA
     4381 AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT
     4441 TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGTCCT
     4501 GGGGCGTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT
     4561 CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT
     4621 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA
     4681 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACT
     4741 TTAGCGACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA
     4801 AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA
     4861 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCA
     4921 AGCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG
     4981 CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACattgc tgcagcggcc gaattcaagc
     5041 gtgaGgagca agcaaggaaa gctaaggtga acaatgagaa aaagacggaa atagtgaaac
     5101 cagagagttg tagcaatgaa ggagatgtca aggatctgaa aagaaaggac tctgaggatg
     5161 gaaacgaggg tgaggaagaa gaagcttctt cgaaaccgaa aaagccaaaa gttgctcttt
     5221 ctcatcttca ggacattgac gacacagaag ctgatcaaga agaagagtaa GGtgtcggta
     5281 cgctgaaatc accagtctct ctctacaaat ctatctctct ctattttctc cataaataat
     5341 gtgtgagtag tttcccgata agggaaatta gggttcttat agggtttcgc tcatgtgttg
     5401 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
     5461 tctaattcct aaaaccaaaa tccagtacta aaatccagat cgatgggcta gagcggacta
     5521 gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     5581 attgcgcgct agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5641 caggaaagaa catgaaggcc ttgacaggat atattggcgg gtaaactaag tcgctgtatg
     5701 tgtttgtttg agatctcatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5761 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5821 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5881 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5941 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6001 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6061 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6121 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6181 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6241 tgctgaagcc agttaccttc ggaagaagag ttggtagctc ttgatccggc aaacaaacca
     6301 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6361 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6421 gttaagggat tttggtcatg aattcttgtt agacattatt tgccgactac cttggtgatc
     6481 tcgcctttca cgtagtgaac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
     6541 tcttgtccaa gataagcctg cctagcttca agtatgacgg gctgatactg ggccggcagg
     6601 cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg
     6661 taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc
     6721 gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga
     6781 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc
     6841 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca
     6901 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg
     6961 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa
     7021 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg
     7081 gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag
     7141 ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc
     7201 tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt taactcctga
     7261 attaagccgc gccgcgaagc ggtgtggctt gaatgaattg ttaggcgtca tcctgtgctc
     7321 ccgagaacca gtaccagtac atcgctgttt cgttcgagac ttgaggtcta gttttatacg
     7381 tgaacaggtc aatgccgccg agagtaaagc cacatttgcg tacaaattgc aggcaggtac
     7441 attgttcgtt tgtgtctcta atcgtatgcc aaggagctgt ctgcttagtg cccacttttt
     7501 cgcaaaattc gatgagactg tgcgcgactc ctttgcctcg gtgcgtgtgc gacacaacaa
     7561 tgtgttcgat agaggctaga tcgttcccat gttgagttga gttcaatctt cccgacaagc
     7621 tcttggtcga tgaatgtgcc atagcaagca gagtcttcat cagagtcatc atccgagatg
     7681 taatccttcc ggtaggggct cacacttctg gtagatagtt caaagccttg gtcggatagg
     7741 tgcacatcga acacttcacg aacaatgaaa tggttctcag catccaatgt ttccgccacc
     7801 tgctcaggga tcaccgaaat cttcatatga cgcctaacgc ctggcacagc ggatcgcaaa
     7861 cctggcgcgg cttttggcac aaaaggcgtg acaggtttgc gaatccgttg ctgccacttg
     7921 tttaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7981 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggatctc gcggtatcat
     8041 tgcagcactg ccatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     8101 acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc
     8161 atcttcccga caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt
     8221 ccacctacaa caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg
     8281 cgattcaggc gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag
     8341 cctgtggata gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc
     8401 acggggcttt ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg
     8461 aaatcgctaa tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa
     8521 aggcacgtga gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc
     8581 ggcaagtagt tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc
     8641 aattattggt cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga
     8701 caaccgcaag cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca
     8761 acagatcgtt ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc
     8821 tcgggcttct ggatttccga tccccggaat tagact  
//
