LOCUS       Exported               12233 bp ds-DNA     circular SYN 14-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 2, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12233
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..512
                     /label=URA3 5' Homology
     misc_feature    complement(513..532)
                     /label=URA3 5' barcode
     misc_feature    552..576
                     /label=ConS scar
                     /label=ConS scar
     misc_feature    552..555
                     /label=BsmBI
                     /label=BsmBI
     protein_bind    complement(552..555)
                     /label=BsmBI
                     /label=BsmBI(1)
     misc_feature    581..1280
                     /label=ScPGK1 Promoter
                     /label=ScPGK1 Promoter
     misc_feature    complement(1611..1614)
                     /label=Bbs1
                     /label=Bbs1
     misc_feature    complement(1617..1622)
                     /label=Bbs1
                     /label=Bbs1
     misc_feature    1881..1886
                     /label=Bbs1
                     /label=Bbs1
     misc_feature    1889..1892
                     /label=Bbs1
                     /label=Bbs1
     misc_feature    2919..2927
                     /label=PTS1
                     /label=PTS1
     misc_feature    2944..3168
                     /label=ScENO1 Terminator
                     /label=ScENO1 Terminator
     misc_feature    2944..3168
                     /label=Sc tENO1
                     /label=Sc tENO1
     misc_feature    3173..3197
                     /label=Con1 scar
     misc_feature    3173..3176
                     /label=ConR1
                     /label=ConR1
     protein_bind    3173..3176
                     /label=BsmBI
     misc_feature    complement(3173..3176)
                     /label=BsmBI
                     /label=BsmBI
     protein_bind    3198..3201
                     /label=BsaI
     protein_bind    complement(3198..3201)
                     /label=BsaI
                     /label=BsaI(1)
     promoter        3202..3877
                     /label=ScHHF2 Promoter
     misc_feature    3883..3886
                     /label=BsaI
                     /label=BsaI
     misc_feature    3883..3886
                     /label=BsaI
                     /label=BsaI
     protein_bind    complement(3883..3886)
                     /label=BsaI
                     /label=BsaI(1)
     misc_feature    3959..3964
                     /label=Bbs1
                     /label=Bbs1
     misc_feature    3967..3970
                     /label=Bbs1
                     /label=Bbs1
     misc_feature    complement(4750..4753)
                     /label=BsaI
                     /label=BsaI
     misc_feature    complement(4750..4753)
                     /label=BsaI
                     /label=BsaI
     CDS             4760..4786
                     /codon_start=1
                     /label=ePTS1
                     /translation="LGRGRRSKL"
     protein_bind    4799..4802
                     /label=BsaI
     terminator      4803..5027
                     /label=ScADH1 Terminator
     protein_bind    5028..5031
                     /label=BsaI
     protein_bind    complement(5028..5031)
                     /label=BsaI
                     /label=BsaI(1)
     protein_bind    5032..5035
                     /label=BsmBI
     protein_bind    complement(5032..5035)
                     /label=BsmBI
     misc_feature    5036..5056
                     /label=Con2 scar
     protein_bind    5057..5060
                     /label=BsaI
     protein_bind    complement(5057..5060)
                     /label=BsaI
                     /label=BsaI(1)
     promoter        5061..5760
                     /label=ScCCW12 Promoter
     protein_bind    complement(5766..5769)
                     /label=BsaI
                     /label=BsaI(1)
     CDS             5770..6822
                     /label=ScErg20p(F96W_N127W)
     CDS             6835..6861
                     /codon_start=1
                     /label=ePTS1
                     /translation="LGRGRRSKL"
     protein_bind    6874..6877
                     /label=BsaI
     terminator      6878..7102
                     /label=ScSSA1 Terminator
     protein_bind    7103..7106
                     /label=BsaI
     protein_bind    complement(7103..7106)
                     /label=BsaI
                     /label=BsaI(1)
     misc_feature    7107..7131
                     /label=Con3 scar
     protein_bind    7107..7110
                     /label=BsmBI
     protein_bind    complement(7107..7110)
                     /label=BsmBI
     protein_bind    7132..7135
                     /label=BsaI
     protein_bind    complement(7132..7135)
                     /label=BsaI
                     /label=BsaI(1)
     promoter        7136..7835
                     /label=ScRPL18B Promoter
     protein_bind    7841..7844
                     /label=BsaI
     protein_bind    complement(7841..7844)
                     /label=BsaI
                     /label=BsaI(1)
     CDS             7845..8555
                     /codon_start=1
                     /label=Venus
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     protein_bind    complement(8558..8561)
                     /label=BsaI
                     /label=BsaI(1)
     CDS             8568..8594
                     /codon_start=1
                     /label=ePTS1
                     /translation="LGRGRRSKL"
     protein_bind    8607..8610
                     /label=BsaI
     terminator      8611..8835
                     /label=ScENO2 Terminator
     protein_bind    8836..8839
                     /label=BsaI
     protein_bind    complement(8836..8839)
                     /label=BsaI
                     /label=BsaI(1)
     misc_feature    8840..8864
                     /label=ConE scar
     protein_bind    8840..8843
                     /label=BsmBI
     protein_bind    complement(8840..8843)
                     /label=BsmBI
     promoter        8884..9060
                     /label=URA3 Promoter
     CDS             9061..9864
                     /codon_start=1
                     /label=URA3
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      9865..9942
                     /label=URA3 Terminator
     misc_feature    9947..9966
                     /label=URA3 3' barcode
     misc_feature    9967..10466
                     /label=URA3 3' Homology
     terminator      complement(10479..10505)
                     /label=KanR Terminator
     CDS             complement(10506..11321)
                     /codon_start=1
                     /label=KanR
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(11322..11469)
                     /label=KanR Promoter
     rep_origin      complement(11470..12233)
                     /direction=LEFT
                     /label=ColE1
ORIGIN
        1 gcggccgcca atgctaaatt cgagtgaaac acaggaagat cagaaaatcc tcatttcatc
       61 catattaaca ataatttcaa atgtttattt gcattatttg aaactaggca agacaagcaa
      121 cgaaacgttt ttgaaaattt tgagtatttt caataaattt gtagaggact cagatattga
      181 aaaaaagcta cagcaattaa tacttgataa gaagagtatt gagaagggca acggttcatc
      241 atctcatgga tctgcacatg aacaaacacc agagtcaaac gacgttgaaa ttgaggctac
      301 tgcgccaatt gatgacaata cagacgatga taacaaaccg aagttatctg atgtagaaaa
      361 ggattaaaga tgctaagaga tagtgatgat atttcataaa taatgtaatt ctatatatgt
      421 taattacctt ttttgcgagg catatttatg gtgaaggata agttttgacc atcaaagaag
      481 gttaatgtgg ctgtggtttc agggtccata aagcccacat ggataacatt acccctgaat
      541 tcgcatctag actgaactgg ccgataattg cagacgaacg gtgagtaagg aaagagtgag
      601 gaactatcgc atacctgcat ttaaagatgc cgatttgggc gcgaatcctt tattttggct
      661 tcaccctcat actattatca gggccagaaa aaggaagtgt ttccctcctt cttgaattga
      721 tgttaccctc ataaagcacg tggcctctta tcgagaaaga aattaccgtc gctcgtgatt
      781 tgtttgcaaa aagaacaaaa ctgaaaaaac ccagacacgc tcgacttcct gtcatcctat
      841 tgattgcagc ttccaatttc gtcacacaac aaggtcctag cgacggctca caggttttgt
      901 aacaagcaat cgaaggttct ggaatggcgg gaaagggttt agtaccacat gctatgatgc
      961 ccactgtgat ctccagagca aagttcgttc gatcgtactg ttactctctc tctttcaaac
     1021 agaattgtcc gaatcgtgtg acaacaacag cctgttctca cacactcttt tcttctaacc
     1081 aagggggtgg tttagtttag tagaacctcg tgaaacttac atttacatat atataaactt
     1141 gcataaattg gtcaatgcaa gaaatacata tttggtcttt tctaattcgt agtttttcaa
     1201 gttcttagat gctttctttt tctctttttt acagatcatc aaggaagtaa ttatctactt
     1261 tttacaacaa atataaaaca agatctatga tcgctgcccc attagttaga gataacggtt
     1321 cagcattgaa cttcttccca caagccccac aagtagaaat cgatgagtca tcaagaataa
     1381 tggagttagt tgaagcaacc aggagaactt taagaaatga atcatctgat tctactgaga
     1441 aaatgagatt gatcgactct ttgcaaagat taggtttgaa ccatcacttt gaacaagata
     1501 taaaagaaat gttacaggat tttgcaaacg aacacaagaa taccaatcaa gatttgttca
     1561 ctacatcttt gagatttagg ttgttaagac acaatggttt caatgtaaca ccagacgtct
     1621 tcaataaatt taccgaggaa aatggtaagt tcaaggaatc attaggtgag gatacaatcg
     1681 gaatattgtc tttatacgaa gcctcttatt taggaggtaa aggtgaagag attttatctg
     1741 aggctatgaa gttttctgaa tctaaattga gggaatcttc tggacacgta gcaccacata
     1801 tcagaaggca aatatttcag tcattggaat tgccaaggca tttgaggatg gctagattag
     1861 aatcaagaag gtacatcgaa gaagactact ctaacgaaat cggagccgat tcatcattat
     1921 tagagttagc taaattggat ttcaattctg ttcaagcatt gcatcaaatg gaattaactg
     1981 agattagtag atggtggaag cagttaggtt tgtctgataa attacctttc gcaagagaca
     2041 gacctttgga gtgcttctta tggactgtag gattattacc agaaccaaaa tactcaggtt
     2101 gcagaatcga gttggccaag actattgctg ttttgttggt tattgacgac atctttgaca
     2161 cctatggatc ttatgatcag ttgatattat ttacaaacgc tataagacgt tgggatttag
     2221 acgccatgga cgagttgcct gaatatatga aaatctgtta catggcatta tacaacacta
     2281 caaatgagat atgttataaa gttttgaaag aaaatggatg gtctgtatta ccttacttag
     2341 agagaacctg gattgacatg gttgagggtt ttatgttaga ggctaaatgg ttaaactcag
     2401 gtgagcaacc aaatttagaa gcttatatag aaaacggtgt aaccacagct ggatcataca
     2461 tggctcttgt tcatctcttc tttcttatag gtgacggagt taatgatgaa aacgtaaaat
     2521 tgttattgga cccatatcca aagttattct catctgccgg tagaattttg agattatggg
     2581 acgatttagg aactgcaaag gaagagcaag aaaggggtga cgtatcatct tcaatccaat
     2641 tatacatgaa ggagaagaat gttaggtcag aatctgaagg aagggagggt atagtcgaga
     2701 ttatttacaa tttgtggaaa gatatgaatg gtgagttaat tggttctaat gctttgccac
     2761 aagccattat tgagacatct tttaacatgg cgagaacttc tcaagtagtc taccaacacg
     2821 aggatgacac atacttttct tctgttgata attacgtcca atcattgttc ttcacaccag
     2881 tatctgtttc tgttggatcc ttgggaagag gtagaagatc caaattgtaa taactcgagt
     2941 ggcagctttt gattaagcct tctagtccaa aaaacacgtt tttttgtcat ttatttcatt
     3001 ttcttagaat agtttagttt attcatttta tagtcacgaa tgttttatga ttctatatag
     3061 ggttgcaaac aagcattttt cattttatgt taaaacaatt tcaggtttac cttttattct
     3121 gcttgtggtg acgcgtgtat ccgcccgctc ttttggtcac ccatgtatgc tgccaaacca
     3181 gatgtcaaca cagctacaac gtgtggagtg tttgcttgga ttctttagta aaaggggaag
     3241 aacagttgga agggccaaag tggaagtcac aaaacagtgg tcctatataa aagaacaaga
     3301 aaaagattat ttatatacaa ctgcggtcac aagaagcaac gcgagagagc acaacacgct
     3361 gttatcacgc aaactatgtt ttgacaccga gccatagccg tgattgtgcg tcacattggg
     3421 cgataatgaa cgctaaatga ccaactccca tccgtaggag ccccttaggg cgtgccaata
     3481 gtttcacgcg cttaatgcga agtgctcgga acggacaact gtggtcgttt ggcaccggga
     3541 aagtggtact agaccgagag tttcgcattt gtatggcagg acgttctggg agcttcgcgt
     3601 ctaaagcttt ttcgggcgcg aaatgcagac cagaccagaa caaaacaact gacaagaagg
     3661 cgtttaattt aatatgttgt tcactcgcgc ctgggctgtt gttattcggc tagatacata
     3721 cgtgtttgtg cgtatgtagt tatatcatat ataagtatat taggatgagg cggtgaaaga
     3781 gatttttttt ttttcgctta atttattctt ttctctatct tttttcctac atcttgttca
     3841 aaagagtagc aaaaacaaca atcaatacaa taaaataaga tctatgactg ccgacaacaa
     3901 tagtatgccc catggtgcag tatctagtta cgccaaatta gtgcaaaacc aaacacctga
     3961 agacattttg gaagagtttc ctgaaattat tccattacaa caaagaccta atacccgatc
     4021 tagtgaaacg tcaaatgacg aaagcggaga aacatgtttt tctggtcatg atgaggagca
     4081 aattaagtta atgaatgaaa attgtattgt tttggattgg gacgataatg ctattggtgc
     4141 cggtaccaag aaagtttgtc atttaatgga aaatattgaa aagggtttac tacatcgtgc
     4201 attctccgtc tttattttca atgaacaagg tgaattactt ttacaacaaa gagccactga
     4261 aaaaataact ttccctgatc tttggactaa cacatgctgc tctcatccac tatgtattga
     4321 tgacgaatta ggtttgaagg gtaagctaga cgataagatt aagggcgcta ttactgcggc
     4381 ggtgagaaaa ctagatcatg aattaggtat tccagaagat gaaactaaga caaggggtaa
     4441 gtttcacttt ttaaacagaa tccattacat ggcaccaagc aatgaaccat ggggtgaaca
     4501 tgaaattgat tacatcctat tttataagat caacgctaaa gaaaacttga ctgtcaaccc
     4561 aaacgtcaat gaagttagag acttcaaatg ggtttcacca aatgatttga aaactatgtt
     4621 tgctgaccca agttacaagt ttacgccttg gtttaagatt atttgcgaga attacttatt
     4681 caactggtgg gagcaattag atgacctttc tgaagtggaa aatgacaggc aaattcatag
     4741 aatgctagga tccggttcat tgggaagagg tagaagatcc aaattgtaat aactcgagtg
     4801 gcgcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
     4861 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
     4921 ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca
     4981 cctctaccgg catgccgagc aaatgcctgc aaatcgctcc ccatttcgct ggatgcacac
     5041 actggcttaa gatgacaacg cacccatgaa ccacacggtt agtccaaaag gggcagttca
     5101 gattccagat gcgggaatta gcttgctgcc accctcacct cactaacgct gcggtgtgcg
     5161 gatacttcat gctatttata gacgcgcgtg tcggaatcag cacgcgcaag aaccaaatgg
     5221 gaaaatcgga atgggtccag aactgctttg agtgctggct attggcgtct gatttccgtt
     5281 ttgggaatcc tttgccgcgc gcccctctca aaactccgca caagtcccag aaagcgggaa
     5341 agaaataaaa cgccaccaaa aaaaaaaaaa taaaagccaa tcctcgaagc gtgggtggta
     5401 ggccctggat tatcccgtac aagtatttct caggagtaaa aaaaccgttt gttttggaat
     5461 ttcccatttc gcggccacct acgccgctat ctttgcaaca actatctgcg ataactcagc
     5521 aaattttgca tattcgtgtt gcagtattgc gataatggga gtcttacttc caacataacg
     5581 gcagaaagaa atgtgagaaa attttgcatc ctttgcctcc gttcaagtat ataaagtcgg
     5641 catgcttgat aatctttctt tccatcctac attgttctaa ttattcttat tctcctttat
     5701 tctttcctaa cataccaaga aattaatctt ctgtcattcg cttaaacact atatcaataa
     5761 agatctatgg cttcagaaaa agaaattagg agagagagat tcttgaacgt tttccctaaa
     5821 ttagtagagg aattgaacgc atcgcttttg gcttacggta tgcctaagga agcatgtgac
     5881 tggtatgccc actcattgaa ctacaacact ccaggcggta agctaaatag aggtttgtcc
     5941 gttgtggaca cgtatgctat tctctccaac aagaccgttg aacaattggg gcaagaagaa
     6001 tacgaaaagg ttgccattct aggttggtgc attgagttgt tgcaggctta ctggttggtc
     6061 gccgatgata tgatggacaa gtccattacc agaagaggcc aaccatgttg gtacaaggtt
     6121 cctgaagttg gggaaattgc catctgggac gcattcatgt tagaggctgc tatctacaag
     6181 cttttgaaat ctcacttcag aaacgaaaaa tactacatag atatcaccga attgttccat
     6241 gaggtcacct tccaaaccga attgggccaa ttgatggact taatcactgc acctgaagac
     6301 aaagtcgact tgagtaagtt ctccctaaag aagcactcct tcatagttac tttcaagact
     6361 gcttactatt ctttctactt gcctgtcgca ttggccatgt acgttgccgg tatcacggat
     6421 gaaaaggatt tgaaacaagc cagagatgtc ttgattccat tgggtgaata cttccaaatt
     6481 caagatgact acttagactg cttcggtacc ccagaacaga tcggtaagat cggtacagat
     6541 atccaagata acaaatgttc ttgggtaatc aacaaggcat tggaacttgc ttccgcagaa
     6601 caaagaaaga ctttagacga aaattacggt aagaaggact cagtcgcaga agccaaatgc
     6661 aaaaagattt tcaatgactt gaaaattgaa cagctatacc acgaatatga agagtctatt
     6721 gccaaggatt tgaaggccaa aatttctcag gtcgatgagt ctcgtggctt caaagctgat
     6781 gtcttaactg cgttcttgaa caaagtttac aagagaagca aaggatccgg ttcattggga
     6841 agaggtagaa gatccaaatt gtaataactc gagtggcgcc aattggtgcg gcaattgata
     6901 ataacgaaaa tgtcttttaa tgatctgggt ataatgagga attttccgaa cgtttttact
     6961 ttatatatat atatacatgt aacatatatt ctatacgcta tagagaaagg aaatttttca
     7021 attaaaaaaa aaatagagaa agagtttcac ttcttgatta tcgctaacac taatggttga
     7081 agtactgcta ctttaatttt atgctggttc tgtatgggca cagacaacct aaacgaagag
     7141 gatgtccaat atttttttta aggaataagg atacttcaag actagattcc cccctgcatt
     7201 cccatcagaa ccgtaaacct tggcgctttc cttgggaagt attcaagaag tgccttgtcc
     7261 ggtttctgtg gctcacaaac cagcgcgccc gatatggctt tcttttcact tatgaatgta
     7321 ccagtacggg acaattagaa cgctcctgta acaatctctt tgcaaatgtg gggttacatt
     7381 ctaaccatgt cacactgctg acgaaattca aagtaaaaaa aaatgggacc acgtcttgag
     7441 aacgatagat tttctttatt ttacattgaa cagtcgttgt ctcagcgcgc tttatgtttt
     7501 cattcatact tcatattata aaataacaaa agaagaattt catattcacg cccaagaaat
     7561 caggctgctt tccaaatgca attgacactt cattagccat cacacaaaac tctttcttgc
     7621 tggagcttct tttaaaaaag acctcagtac accaaacacg ttacccgacc tcgttatttt
     7681 acgacaacta tgataaaatt ctgaagaaaa aataaaaaaa ttttcatact tcttgctttt
     7741 atttaaacca ttgaatgatt tcttttgaac aaaactacct gtttcaccaa aggaaataga
     7801 aagaaaaaat caattagaag aaaacaaaaa acaaaagatc tatgtctaaa ggtgaagaat
     7861 tattcactgg tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat
     7921 tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg taaattgacc ttaaaattga
     7981 tttgtactac tggtaaattg ccagttccat ggccaacctt agtcactact ttaggttatg
     8041 gtttgcaatg ttttgctaga tacccagatc atatgaaaca acatgacttt ttcaagtctg
     8101 ccatgccaga aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca
     8161 agaccagagc tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag
     8221 gtattgattt taaagaaggt ggtaacattt taggtcacaa attggaatac aactataact
     8281 ctcacaatgt ttacatcact gctgacaaac aaaagaatgg tatcaaagct aacttcaaaa
     8341 ttagacacaa cattgaagat ggtggtgttc aattagctga ccattatcaa caaaatactc
     8401 caattggtga tggtccagtc ttgttaccag acaaccatta cttatcctat caatctgcct
     8461 tatccaaaga tccaaacgaa aagagagatc acatggtctt gttagaattt gttactgctg
     8521 ctggtattac ccatggtatg gatgaattgt acaaaggatc cggttcattg ggaagaggta
     8581 gaagatccaa attgtaataa ctcgagtggc agtgctttta actaagaatt attagtcttt
     8641 tctgcttatt ttttcatcat agtttagaac actttatatt aacgaatagt ttatgaatct
     8701 atttaggttt aaaaattgat acagttttat aagttacttt ttcaaagact cgtgctgtct
     8761 attgcataat gcactggaag gggaaaaaaa aggtgcacac gcgtggcttt ttcttgaatt
     8821 tgcagtttga aaaatgctga gcagttacag agatgttacg aaccactagt gcactgcagt
     8881 acacggtttc cttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg
     8941 aaggagcaca gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat
     9001 tgcccagtat tcttaaccca actgcacaga acaaaaacgt gcaggaaacg aagataaatc
     9061 atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag
     9121 ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc
     9181 accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca
     9241 catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta
     9301 tccgccaagt acaatttttt actcttcgag gacagaaaat ttgctgacat tggtaataca
     9361 gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat
     9421 gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta
     9481 acaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct
     9541 actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc
     9601 ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg
     9661 acacccggtg tgggtttaga tgacaaggga gatgcattgg gtcaacagta tagaaccgtg
     9721 gatgatgtgg tttctacagg atctgacatt attattgttg gaagaggact atttgcaaag
     9781 ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg
     9841 agaagatgcg gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac
     9901 tcacaaatta gagcttcaat ttaattatat cagttattac ccgagtagag cacttgaatc
     9961 cactgccccg ggaatctcgg tcgtaatgat ttctataatg acgaaaaaaa aaaaattgga
    10021 aagaaaaagc ttcatggcct ttataaaaag gaactatcca atacctcgcc agaaccaagt
    10081 aacagtattt tacggggcac aaatcaagaa caataagaca ggactgtaaa gatggacgca
    10141 ttgaactcca aagaacaaca agagttccaa aaagtagtgg aacaaaagca aatgaaggat
    10201 ttcatgcgtt tgtactctaa tctggtagaa agatgtttca cagactgtgt caatgacttc
    10261 acaacatcaa agctaaccaa taaggaacaa acatgcatca tgaagtgctc agaaaagttc
    10321 ttgaagcata gcgaacgtgt agggcagcgt ttccaagaac aaaacgctgc cttgggacaa
    10381 ggcttgggcc gataaggtgt actggcgtat atatatctaa ttatgtatct ctggtgtagc
    10441 ccatttttag catgtaaata taaagaccga gcggccgcgt gttacaacca attaaccaat
    10501 tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
    10561 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
    10621 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
    10681 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
    10741 acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca
    10801 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
    10861 gattgcgcct gagcgaggcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
    10921 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
    10981 ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat
    11041 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
    11101 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
    11161 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
    11221 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
    11281 cgcggcctgg agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg
    11341 tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa
    11401 catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg
    11461 aaggatcagt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    11521 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    11581 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    11641 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    11701 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    11761 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    11821 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    11881 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11941 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    12001 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    12061 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    12121 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    12181 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgt
//
