LOCUS       Exported               10674 bp ds-DNA     circular SYN 12-DEC-2021
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...el-bho8z5c
VERSION     .
KEYWORDS    FUGW Puro_P2A_Cox8A_Timer
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 2, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Alias: FUW-linker(digested by EcoRI, BamHI)Fragtment2-Puro T2A no 
            KOZAK- LORDmitotimer
FEATURES             Location/Qualifiers
     source          1..10674
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4549..5346
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /label=pRS-marker
     promoter        212..816
                     /standard_name="CMV\promoter/enhancer"
                     /label=CMV promoter/enhancer promoter
     misc_feature    238..617
                     /label=CMV enhancer
     promoter        619..817
                     /standard_name="CMV promoter"
                     /label=CMV promoter
                     /note="CMV promoter; human cytomegalovirus (CMV) immediate
                     early  promoter"
     primer_bind     772..792
                     /label=CMV-F
     misc_feature    835..1509
                     /label=5' HIVR-U5
     LTR             835..1015
                     /standard_name="5' LTR (truncated)"
                     /label=5' LTR (truncated)
                     /note="5' LTR (truncated); truncated 5' long terminal
                     repeat (LTR) from HIV-1"
     primer_bind     1017..1034
                     /standard_name="primer_bind"
     misc_feature    1062..1187
                     /label=HIV-1 psi
     primer_bind     1289..1311
                     /standard_name="GAGfor"
                     /label=GAGfor
     primer_bind     complement(1345..1369)
                     /standard_name="GAGrev"
                     /label=GAGrev
     misc_feature    1680..1913
                     /standard_name="RRE"
                     /label=RRE
                     /note="RRE; The Rev response element (RRE) of HIV-1 allows
                     for Rev-dependent mRNA export from the nucleus to 
                     thecytoplasm."
     CDS             2098..2445
                     /codon_start=1
                     /label=gp41 peptide
                     /translation="KNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLRIVF
                     AVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVNGSALRAPI
                     LQTNGSIHPQF"
     primer_bind     2321..2340
                     /standard_name="Promotorseqfor"
                     /label=Promotorseqfor
     primer_bind     2401..2420
                     /standard_name="cppt; xxxxxx"
                     /label=cppt
     misc_feature    2440..2557
                     /standard_name="cPPT/CTS"
                     /label=cPPT/CTS
                     /note="cPPT/CTS; central polypurine tract and central 
                     termination  sequence of HIV-1"
     promoter        2622..3833
                     /standard_name="UbC promoter"
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     promoter        2622..3144
                     /standard_name="human\ubiquitin\promoter/enhancer"
                     /label=human ubiquitin promoter/enhancer promoter
     primer_bind     complement(2709..2726)
                     /standard_name="UBI seq rev"
                     /label=UBI seq rev
     primer_bind     complement(2837..2855)
                     /standard_name="UBC seq reverse to look for insert"
                     /label=UBC seq reverse to look for insert
     intron          3145..3833
                     /standard_name="human\ubiquitin\intron"
                     /label=human ubiquitin intron
     ligation        3869..3885
                     /standard_name="Ligation"
     CDS             3886..4482
                     /codon_start=1
                     /standard_name="puro"
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     3886..3905
                     /standard_name="Puro T2A no KOZAK (1 F) - FUW-linker
                     (digested by EcoRI, BamHI) Fragment 2"
     primer_bind     4031..4050
                     /label=Primer 1
     primer_bind     complement(4202..4220)
                     /label=Primer 2
     CDS             4483..4548
                     /codon_start=1
                     /standard_name="2A"
                     /label=P2A
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     primer_bind     complement(4530..4548)
                     /standard_name="Puro T2A no KOZAK (663 R) - LORD
                     mitotimer"
                     /label=Puro T2A no KOZAK (663 R)
     primer_bind     4549..4572
                     /standard_name="LORD mitotimer (1 F) - Puro T2A no KOZAK"
                     /label=Puro T2A no KOZAK (1F)
     CDS             4561..4647
                     /codon_start=1
                     /label=COX8A Sig
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAKIHSL"
     CDS             4669..5346
                     /codon_start=1
                     /standard_name="Timer"
                     /product="green-to-red fluorescent timer derivative of
                     DsRed (Terskikh et al., 2000)"
                     /label=Timer
                     /note="mammalian codon-optimized"
                     /translation="VRSSKNVIKESMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(5327..5346)
                     /standard_name="LORD mitotimer (798 R) - FUW-linker
                     (digested by EcoRI, BamHI) Fragment 2"
     ligation        5347..5363
                     /standard_name="Ligation"
     misc_feature    5366..5954
                     /standard_name="WRE"
                     /label=WPRE
     primer_bind     5366..5385
                     /standard_name="Digestion of TET CMV  U Puro T2a M2 -
                     Fragment 1 - WPRE (1 F) - F2A Neomycin use CPF1 VPR as 
                     template; Digestion of TET CMV  U Puro T2a M2 - Fragment 1 
                     - WPRE (1 F) - Ligation of AAVS1-SA-2A-NEO-CAG-RTTA3 
                     extraction - Digestion of TET CMV  U Puro T2a M2 - Fragment
                     1 - WPRE into Digestion of Ligation of G1211: CAG-human 
                     dLbCpf1(D832A)-NLS-3xHA-VPR extraction into Digestion of 
                     TET CMV  U Puro T2a M2 - Fragment 2 - 
                     AAVS1-SA-2A-NEO-CAG-RTTA3 extraction (concatenated sequence
                     2); Digestion of TET CMV  U Puro T2a M2 - Fragment 1 - WPRE
                     (1 F) - Neomycin from any vector maybe AAVSI vector or 
                     reamplify old PCR in box"
     primer_bind     complement(5419..5439)
                     /label=WPRE-R
     primer_bind     complement(5476..5495)
                     /standard_name="PDL38seqPrimer"
                     /label=PDL38seqPrimer
     primer_bind     complement(5630..5649)
                     /standard_name="WPRE rev"
                     /label=WPRE rev
     primer_bind     5661..5680
                     /label=Primer 3
     CDS             complement(5837..5848)
                     /codon_start=1
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5957..5973)
                     /standard_name="KS primer"
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5958..5974)
                     /label=pBlueScriptKS
     misc_feature    5970..6665
                     /label=HIV delU3RdelU5
     misc_feature    6479..6659
                     /label=5' LTR (truncated)
     primer_bind     complement(6685..6702)
                     /label=BGH-R
     polyA_signal    6691..6884
                     /note="bGH poly(A) signal; bovine growth hormone
                     polyadenylation signal"
                     /note="/standard_name=bGH poly(A) signal"
     rep_origin      6961..7389
                     /label=f1 origin
     primer_bind     complement(7048..7067)
                     /label=F1ori-R
     primer_bind_reversecomplement(7232..7251)
                     /direction=LEFT
                     /standard_name="1,909 R"
     primer_bind     7258..7279
                     /label=F1ori-F
     primer_bind     7321..7340
                     /standard_name="F1ORIREPSEQ"
                     /label=F1ORIREPSEQ
     primer_bind     complement(7398..7418)
                     /label=pBABE 3'
     promoter        7403..7732
                     /standard_name="SV40 promoter"
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter; SV40 promoter"
     primer_bind     7567..7586
                     /standard_name="SV40-Promoter"
                     /label=SV40-Promoter
     rep_origin      7583..7718
                     /label=SV40 ori
     primer_bind     7645..7664
                     /label=SV40pro-F
     promoter        7778..7845
                     /standard_name="EM7 prom"
                     /label=EM7 prom promoter
     CDS             7846..8220
                     /codon_start=1
                     /standard_name="ZEO"
                     /label=BleoR
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    8350..8471
                     /label=SV40 poly(A) signal
     primer_bind     complement(8387..8406)
                     /label=SV40pA-R
     primer_bind     8441..8460
                     /label=EBV-RP
     primer_bind     complement(8520..8536)
                     /label=M13 rev
     primer_bind     complement(8533..8554)
                     /label=M13/pUC Reverse
     misc_feature    8544..8560
                     /label=lac operator
     promoter        complement(8568..8598)
                     /standard_name="lac promoter"
                     /label=lac promoter
                     /note="lac promoter; promoter for the E. coli lac operon"
     protein_bind    complement(8613..8634)
                     /standard_name="CAP binding site"
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind_reversecomplement(8803..8822)
                     /direction=LEFT
                     /standard_name="VR"
     rep_origin      8899..9499
                     /label=pUC origin
     primer_bind     complement(9002..9021)
                     /label=pBR322ori-F
     primer_bind_reversecomplement(9061..9080)
                     /direction=LEFT
                     /standard_name="1,388 R"
     primer_bind     9062..9081
                     /standard_name="1,370 F"
                     /label=1,370 F
     CDS             complement(9681..10541)
                     /codon_start=1
                     /gene="bla"
                     /standard_name="bla gene"
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind_reversecomplement(9801..9820)
                     /direction=LEFT
                     /standard_name="512 R"
     primer_bind     9801..9820
                     /standard_name="4,459 F"
                     /label=4,459 F
     primer_bind     10304..10323
                     /label=Amp-R
     promoter        complement(10542..10646)
                     /gene="bla"
                     /standard_name="AmpR promoter"
                     /label=AmpR promoter
                     /note="AmpR promoter; color: #ffffff; direction: RIGHT"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
     2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     3841 gggcccggga attaattcac catgtctaga ttaattaagc tagcgatgac cgagtacaag
     3901 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     3961 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     4021 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     4081 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     4141 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     4201 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     4261 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     4321 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggaaac ctccgcgccc
     4381 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     4441 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccggaagcgg agctactaac
     4501 ttcagcctgt tgaaacaagc aggggatgtc gaagagaatc ccgggccatt gaagacaaaa
     4561 atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca
     4621 gtgccgcgcg ccaagatcca ttcgttgggg gatccccggg taccggtcgt gcgctcctcc
     4681 aagaacgtca tcaaggagtc catgcgcttc aaggtgcgca tggagggcac cgtgaacggc
     4741 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcca caacaccgtg
     4801 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
     4861 ttccagtacg gctccaaggt gtacgtgaag caccccgccg acatccccga ctacaagaag
     4921 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     4981 gcgaccgtga cccaggactc ctccctgcag gacggctgct tcatctacaa ggtgaagttc
     5041 atcggcgtga acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag
     5101 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccacaaggcc
     5161 ctgaagctga aggacggcgg ccactacctg gtggagttca agtccatcta catggccaag
     5221 aagcccgtgc agctgcccgg ctactactac gtggacacca agctggacat cacctcccac
     5281 aacgaggact acaccatcgt ggagcagtac gagcgcaccg agggccgcca ccacctgttc
     5341 ctgtagcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     5401 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5461 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5521 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5581 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5641 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5701 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5761 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5821 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5881 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5941 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     6001 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     6061 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     6121 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6181 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     6241 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     6301 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     6361 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     6421 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     6481 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6541 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6601 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6661 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6721 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6781 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6841 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6901 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     6961 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7021 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7081 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7141 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7201 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7261 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7321 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7381 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7441 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7501 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     7561 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7621 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     7681 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     7741 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     7801 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     7861 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     7921 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     7981 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     8041 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     8101 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     8161 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     8221 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     8281 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     8341 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8401 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8461 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     8521 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8581 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8641 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8701 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8761 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8821 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8881 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8941 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9001 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9061 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9601 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9661 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9721 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9781 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9841 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9901 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9961 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    10021 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10081 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10141 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10201 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10261 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10321 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10381 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10441 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10501 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10561 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10621 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
