LOCUS       pXYB034_split_sfGFP_by 14602 bp ds-DNA     circular     07-JUL-2023
DEFINITION  .
KEYWORDS    "creator:Xiaohan Yang" "marker:NeoR/KanR, KanR, Modified
            COR47-5'-UTR"
FEATURES             Location/Qualifiers
     promoter        complement(16..361)
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     primer_bind     738..754
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     primer          738..754
                     /label="oYL_ORNL048"
                     /note="sequence: gtaaaacgacggccagt"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(773..790)
                     /label="oYL_ORNL_047"
                     /note="sequence: ggctaatctggggacctg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        1269..1614
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     misc_feature    1599..1623
                     /label="overlap1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1624..1730
                     /label="Modified COR47-5'-UTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1706..1735
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1706..3415
                     /label="gYL055"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1739..1934
                     /label="1st half sfGFP (Y66)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1739..2868
                     /label="sfGFP"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLT*IAIFTFGSKSYQACTW*LVFKPIDCIGLKGKTVKLRERGQQPFSTKSQGKL*DGLAKGMVIS*RTWS*PRSQVLSQQIFC*YGCSSQTKCRSGKMYSSHKI*SDLSLMGASG*SDATLEPLGTNLYAKVY*LVLEYSMVFNAFPVIRIT*NGMTFSRVPCPKVMYRNALYLSKMTGPTRRVLKSSLKVIPLLIVSS*RVLILKKMETFLDTNSSTTLTHTMYTSRQTNKRMESKLTSKFATTLKMVPFN*QTIINKILQLAMALSFYQTTITCRHNLSFRKIPTKSVTTWSFLSL*LLLGLHMAWMSSTN"
     misc_feature    1935..1954
                     /label="P1 Loop WT"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1955..1960
                     /label="Internal guide sequence"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1961..2347
                     /label="Ribozyme"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2348..2868
                     /label="2nd half sfGFP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2869..3746
                     /label="HSPT878"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2894..2918
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3380..3415
                     /label="Gibson overhang"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3747..3767
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3798..3822
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(3804..3824)
                     /label="oYL_ORNL029"
                     /note="sequence: caggaaacagctatgaccatg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(3808..3824)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    3832..3848
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3856..3862)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3863..3880)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3881..3886)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    3901..3922
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        4113..4790
                     /label="CaMV 35S promoter (enhanced)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: cauliflower mosaic virus 35S promoter with a duplicated enhancer region"
     misc_feature    4794..5720
                     /label="gBlocks for change to Kan"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4858..5655
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-II (or nptII) note: confers resistance to neomycin, kanamycin, and G418 (Geneticin®) product: aminoglycoside phosphotransferase from Tn5 transl_table: 1 translation: MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     polyA_signal    5695..5869
                     /label="CaMV poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: cauliflower mosaic virus polyadenylation signal"
     misc_feature    5947..5971
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: left border repeat from nopaline C58 T-DNA"
     CDS             6396..7190
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aphA-3 note: confers resistance to kanamycin product: aminoglycoside phosphotransferase protein_id: transl_table: 1 translation: MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"
     rep_origin      7277..7865
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     misc_feature    8051..8191
                     /label="bom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: basis of mobility region from pBR322"
     rep_origin      8535..8729
                     /label="pVS1 oriV"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)"
     CDS             complement(8795..9862)
                     /label="pVS1 RepA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 note: product: replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) protein_id: transl_table: 1 translation: GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     CDS             complement(10296..10925)
                     /label="pVS1 StaA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 product: stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) transl_table: 1 translation: MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
     misc_feature    12225..12249
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: right border repeat from nopaline C58 T-DNA"
     terminator      12271..12523
                     /label="NOS terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: nopaline synthase terminator and poly(A) signal"
     primer          12494..12520
                     /label="oYL072_oNB395 copy"
                     /note="sequence: GAAACTTTATTGCCAAATGTTTGAACG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(12555..12572)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: 6xHis affinity tag transl_table: 1 translation: HHHHHH"
     CDS             complement(12579..14396)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14397..14586
                     /label="cat1 intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: castor bean catalase intron, modified"
     CDS             complement(14397..14586)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     CDS             complement(14587..14601)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actCAAACAT TACTCATTCA CAAAACCATC TTAAAGCAAC TACACAAGTC TTGAAATTTT
     1681 CTCATATTTT CTATTTACTA TATAAACTTT TAATCAAATC AAGATTAAAG TTAATTAAAT
     1741 GAGCAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA
     1801 TGTTAATGGG CACAAATTTT CTGTCCGTGG AGAGGGTGAA GGTGATGCTA CAAACGGAAA
     1861 ACTCACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCGTGGC CAACACTTGT
     1921 CACTACTCTG ACCTAAATAG CAATATTTAC CTTTGGGTCA AAAAGTTATC AGGCATGCAC
     1981 CTGGTAGCTA GTCTTTAAAC CAATAGATTG CATCGGTTTA AAAGGCAAGA CCGTCAAATT
     2041 GCGGGAAAGG GGTCAACAGC CGTTCAGTAC CAAGTCTCAG GGGAAACTTT GAGATGGCCT
     2101 TGCAAAGGGT ATGGTAATAA GCTGACGGAC ATGGTCCTAA CCACGCAGCC AAGTCCTAAG
     2161 TCAACAGATC TTCTGTTGAT ATGGATGCAG TTCACAGACT AAATGTCGGT CGGGGAAGAT
     2221 GTATTCTTCT CATAAGATAT AGTCGGACCT CTCCTTAATG GGAGCTAGCG GATGAAGTGA
     2281 TGCAACACTG GAGCCGCTGG GAACTAATTT GTATGCGAAA GTATATTGAT TAGTTTTGGA
     2341 GTACTCGATG GTGTTCAATG CTTTTCCCGT TATCCGGATC ACATGAAACG GCATGACTTT
     2401 TTCAAGAGTG CCATGCCCGA AGGTTATGTA CAGGAACGCA CTATATCTTT CAAAGATGAC
     2461 GGGACCTACA AGACGCGTGC TGAAGTCAAG TTTGAAGGTG ATACCCTTGT TAATCGTATC
     2521 GAGTTAAAGG GTATTGATTT TAAAGAAGAT GGAAACATTC TTGGACACAA ACTCGAGTAC
     2581 AACTTTAACT CACACAATGT ATACATCACG GCAGACAAAC AAAAGAATGG AATCAAAGCT
     2641 AACTTCAAAA TTCGCCACAA CGTTGAAGAT GGTTCCGTTC AACTAGCAGA CCATTATCAA
     2701 CAAAATACTC CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCGACA
     2761 CAATCTGTCC TTTCGAAAGA TCCCAACGAA AAGCGTGACC ACATGGTCCT TCTTGAGTTT
     2821 GTAACTGCTG CTGGGATTAC ACATGGCATG GATGAGCTCT ACAAATAAAT ATGAAGATGA
     2881 AGATGAAATA TTTGGTGTGT CAAATAAAAA GCTTGTGTGC TTAAGTTTGT GTTTTTTTCT
     2941 TGGCTTGTTG TGTTATGAAT TTGTGGCTTT TTCTAATATT AAATGAATGT AAGATCTCAT
     3001 TATAATGAAT AAACAAATGT TTCTATAATC CATTGTGAAT GTTTTGTTGG ATCTCTTCTG
     3061 CAGCATATAA CTACTGTATG TGCTATGGTA TGGACTATGG AATATGATTA AAGATAAGAT
     3121 GGGCTCATAG AGTAAAACGA GGCGAGGGAC CTATAAACCT CCCTTCATCA TGCTATTTCA
     3181 TGATCTATTT TATAAAATAA AGATGTAGAA AAAAGTAAGC GTAATAACCG CAAAACAAAT
     3241 GATTTAAAAC ATGGCACATA ATGAGGAGAT TAAGTTCGGT TTACGTTTAT TTTAGTACTA
     3301 ATTGTAACGT GAGACTACGT ATCGGGAATC GCCTAATTAA AGCATTAATG CGAACCTGAT
     3361 TAGATTCACC GACCCTCCTA TCGTGTCGAC CTTTCTGTTT CTTAGAATTT TTTGGTAGTC
     3421 TATGTACTAA TAATGTCAGC TTCGTATTTA TTTCATAAGC AATTTGCATT TGCAATTTGT
     3481 TTTTTACTTT TATTTTTATT GTATTGTGGA ATGTGGACTC GTACCAACAT GAAGTTATAT
     3541 ACCACCAAAA AAATTACAGT TAGTCAAAAG ATTCACGAGT GAGAGCTACT TATGATTGTC
     3601 TTTTACGTAT ATGTCTAATT GTCTATTTGC TCAATAATCT TTGTACTTTC TTTTGTCGTT
     3661 GATAAAATCA CAAAGTTCCA AAAGTAATCG AATGATTTGC TTTTAAGAAA AGAAGAGCTC
     3721 AATAATTCAA CATATATCTG TACACAatta ccgcctttga gtgagctcta gatgccacct
     3781 gacgtctaag aagaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     3841 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     3901 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3961 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4021 tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc caagaatatc
     4081 aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg
     4141 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa
     4201 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
     4261 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     4321 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgataacat ggtggagcac
     4381 gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt
     4441 gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc
     4501 tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc
     4561 gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc
     4621 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     4681 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
     4741 gaccttcctc tatataagga agttcatttc atttggagag gacacgctga aatcaccagt
     4801 ctctctctac aaatctatct ctctcgagct ttcgcagatc ccggggggca atgagatatg
     4861 gggattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     4921 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     4981 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactc
     5041 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     5101 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     5161 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     5221 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     5281 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     5341 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     5401 ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     5461 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     5521 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     5581 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5641 gacgagttct tctgaagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc
     5701 cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc
     5761 atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca
     5821 ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa
     5881 ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
     5941 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
     6001 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg
     6061 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc
     6121 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa
     6181 cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact
     6241 atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt
     6301 tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc
     6361 tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg
     6421 aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg
     6481 ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc
     6541 ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag
     6601 gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc
     6661 tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg
     6721 aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg
     6781 attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata
     6841 acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc
     6901 gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg
     6961 gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc
     7021 ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca
     7081 gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc
     7141 ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat
     7201 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7261 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7321 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7381 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     7441 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7501 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7561 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7621 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7681 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7741 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7801 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7861 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7921 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7981 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8041 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     8101 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     8161 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     8221 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     8281 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
     8341 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
     8401 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
     8461 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
     8521 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
     8581 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
     8641 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
     8701 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
     8761 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
     8821 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
     8881 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
     8941 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
     9001 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
     9061 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
     9121 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
     9181 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
     9241 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
     9301 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
     9361 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
     9421 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
     9481 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
     9541 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
     9601 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
     9661 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
     9721 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
     9781 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
     9841 ccgggccgga tggtttgcga ccgtcacgcc gattcctcgg gcttgggggt tccagtgcca
     9901 ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
     9961 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
    10021 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
    10081 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
    10141 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
    10201 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
    10261 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
    10321 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
    10381 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
    10441 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
    10501 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
    10561 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
    10621 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
    10681 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
    10741 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
    10801 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
    10861 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
    10921 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
    10981 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
    11041 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
    11101 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
    11161 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
    11221 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
    11281 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
    11341 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
    11401 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
    11461 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
    11521 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
    11581 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
    11641 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
    11701 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
    11761 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
    11821 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
    11881 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
    11941 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
    12001 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
    12061 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg
    12121 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
    12181 ccttttaaat atccgttatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat
    12241 atcctgtcaa acactgatag tttaattccc gatctagtaa catagatgac accgcgcgcg
    12301 ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt
    12361 gcgggactct aatcataaaa acccatctca taaataacgt catgcattac atgttaatta
    12421 ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag
    12481 gattcaatct taagaaactt tattgccaaa tgtttgaacg atcggggaaa ttcgagctgg
    12541 tcaccaattc acacgtgatg gtgatggtga tggctagcgt tcttgtagcc gaaatctgga
    12601 atgttggtcc agcgctcgcg aaagacgtgc gcggcgagct tcggcttgcg gtcacgagtg
    12661 aacacgccct tcttgtttcc ttggacgcgc atcacgccct gagaggtcgc gaagtccgcg
    12721 aagttccacg cttgctcacc cacgaagttc tcaaactcat cgaacacgac gtggttcgcc
    12781 tggtagtact cgacttgata ttcctcggtg aacatcactg gatcaatgtc gtgaaagccc
    12841 gcaacggtgt ctgcgccgta ctcagtgatc atgatcggct ttcctgggca acgcttgttc
    12901 cacgcgtgaa attcctggcg gagatggact ttggccgctt cgagatcacc gccatcgaag
    12961 taccatccgt tatagcgatt gagcgcgatg acgtcaatca gttcggcgac tttgtccgtc
    13021 tccggggtag ccatcacaaa cagcacgatc gtgaccggac gcttctgtgg gtcgagttcc
    13081 ttggtcagct ccaccaacgg cttgaagtac tcgtacgcgc cctcttcctc agtcgccgcc
    13141 tcgttggcga tgctccacat cacgacgctt ggatggttct tgtcacgaga caccagttca
    13201 cggagaacgt cttgatggtg ctcaaacgtc cgaatcttct cccaggtact gacgcgctcg
    13261 ctgccttcgc cgagtcccgt ggtggccatg aagttgaggt gcacgccaac tgccggagtc
    13321 tcgtcgatca cgaccagacc ctcgcgatcc gcaagacgca tcaactcttc agagtacgga
    13381 tagtgtgcgg tccggaagct gttggcgccg atccatttga ggatattgaa atccatcaca
    13441 ttgctcgctt cgttaaagcc acggccgttg ataggagtgt cctcatgttt gccaaagccc
    13501 ttgaagtaga acggtttgtt gttgatgagg aacttgccgt cgttgacttc cacggtccgc
    13561 acgccgaacg gctcttcata gacatcgatg gtcagtccgt cgttcaccag ttccactttg
    13621 atctggtaga gatacgtgtt cagtggttcc cagaggatga cattcggaat ctccacgtta
    13681 ccgctcaggc cctcggtgct tgcgaccact ttgccttcct catccacgac cgacactttc
    13741 acggtctcgg ctttgccttg aaagtccacc gtataggtca cagtcccggt tgggccattg
    13801 aagtcggtca caaccgagat gtcctcgacg tacgtaaacg gggtcgtgta gattttcacc
    13861 ggacggtgca ggcctgcata gttgaagaag tcgaagttcg gcttgttacg aatgactttt
    13921 ccgaggccct cttcgtggcg ctcgctgtac agccccaccg ggagggtgct atcgtcgagg
    13981 atgttgtcca cggcgacggt gacgcgattc atgccatcac gcagcgagtt gttgatttcc
    14041 gcttcgaatg gcaggaatcc gcccttgtgc tccacgacca gctcaccatt gacatagaca
    14101 attgctttgt gagttgcaga gccgaagcgg agcacgatac gctgatcctt cagataggcc
    14161 ggcaccgtga actcacgttc gtaccagaca tatccgatat ggttgcggat ttccttggtc
    14221 acgccaatgt cattgtaact gcttgggacg gccatactaa tagtgtcggt cagcttgctt
    14281 tcgtaccact tctcttccag tcctttcccg tagtccagct tgaagttcca gacgccattg
    14341 aggtcgaaga cgccacgggt ctcggtgttg atcgggtaca gactagttcg tcggttctgt
    14401 aactatcatc atcatcatag acacacgaaa taaagtaatc agattatcag ttaaagctat
    14461 gtaatattta caccataacc aatcaattaa aaaatagatc agtttaaaga aagatcaaag
    14521 ctcaaaaaaa taaaaagaga aaagggtcct aaccaagaaa atgaaggaga aaaactagaa
    14581 atttaccctc agatctacca tg
//