LOCUS       pXYB031_dRibozyme_spli 14519 bp ds-DNA     circular     07-JUL-2023
DEFINITION  .
KEYWORDS    "creator:Xiaohan Yang" "marker:NeoR/KanR, KanR, Modified
            COR47-5'-UTR"
FEATURES             Location/Qualifiers
     promoter        complement(16..361)
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     primer_bind     738..754
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     primer          738..754
                     /label="oYL_ORNL048"
                     /note="sequence: gtaaaacgacggccagt"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(773..790)
                     /label="oYL_ORNL_047"
                     /note="sequence: ggctaatctggggacctg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        1269..1614
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     misc_feature    1599..1623
                     /label="overlap1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1624..1730
                     /label="Modified COR47-5'-UTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1706..1735
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1706..3332
                     /label="gYL052"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1739..1763
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1739..1954
                     /label="1st half GFPuv"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1739..2785
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MTTFKIESRIHGNLNGEKFELVGGGVGEEGRLEIEMKTKDKPLAFSPFLLTTCMGYGFYHFASFPKGIKNIYKSYQACTW*LVFKPIDCIGLKGKTVKLRERGQQPFSTKSQGKL*DGLAKGMVIS*RTWS*PRSQVLSQQIFC*YGCSSQTKCRSGKMYSSHKI*SDLSLMGASG*SDATLEPLGTNLYAKVY*LVLEYSLHAATNGGYTNTRKEIYEDGGILEVNFRYTYEFNKIIGDVECIGHGFPSQSPIFKDTIVKSCPTVDLMLPMSGNIIASSYAYAFQLKDGSFYTAEVKNNIDFKNPIHESFSKSGPMFTHRRVEETLTKENLAIVEYQQVFNSAPRDM*"
     misc_feature    1769..1793
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1955..2341
                     /label="dRIbozyme"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2342..2785
                     /label="2nd half GFPuv"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2686..2710
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2786..3663
                     /label="HSPT878"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2811..2835
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3297..3332
                     /label="Gibson overhang"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3664..3684
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3715..3739
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(3721..3741)
                     /label="oYL_ORNL029"
                     /note="sequence: caggaaacagctatgaccatg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(3725..3741)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    3749..3765
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3773..3779)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3780..3797)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3798..3803)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    3818..3839
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        4030..4707
                     /label="CaMV 35S promoter (enhanced)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: cauliflower mosaic virus 35S promoter with a duplicated enhancer region"
     misc_feature    4711..5637
                     /label="gBlocks for change to Kan"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4775..5572
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-II (or nptII) note: confers resistance to neomycin, kanamycin, and G418 (Geneticin®) product: aminoglycoside phosphotransferase from Tn5 transl_table: 1 translation: MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     polyA_signal    5612..5786
                     /label="CaMV poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: cauliflower mosaic virus polyadenylation signal"
     misc_feature    5864..5888
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: left border repeat from nopaline C58 T-DNA"
     CDS             6313..7107
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aphA-3 note: confers resistance to kanamycin product: aminoglycoside phosphotransferase protein_id: transl_table: 1 translation: MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"
     rep_origin      7194..7782
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     misc_feature    7968..8108
                     /label="bom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: basis of mobility region from pBR322"
     rep_origin      8452..8646
                     /label="pVS1 oriV"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)"
     CDS             complement(8712..9779)
                     /label="pVS1 RepA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 note: product: replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) protein_id: transl_table: 1 translation: GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     CDS             complement(10213..10842)
                     /label="pVS1 StaA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 product: stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) transl_table: 1 translation: MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
     misc_feature    12142..12166
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: right border repeat from nopaline C58 T-DNA"
     terminator      12188..12440
                     /label="NOS terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: nopaline synthase terminator and poly(A) signal"
     primer          12411..12437
                     /label="oYL072_oNB395 copy"
                     /note="sequence: GAAACTTTATTGCCAAATGTTTGAACG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(12472..12489)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: 6xHis affinity tag transl_table: 1 translation: HHHHHH"
     CDS             complement(12496..14313)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14314..14503
                     /label="cat1 intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: castor bean catalase intron, modified"
     CDS             complement(14314..14503)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     CDS             complement(14504..14518)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actCAAACAT TACTCATTCA CAAAACCATC TTAAAGCAAC TACACAAGTC TTGAAATTTT
     1681 CTCATATTTT CTATTTACTA TATAAACTTT TAATCAAATC AAGATTAAAG TTAATTAAat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctatAAAAGT TATCAGGCAT GCACCTGGTA
     1981 GCTAGTCTTT AAACCAATAG ATTGCATCGG TTTAAAAGGC AAGACCGTCA AATTGCGGGA
     2041 AAGGGGTCAA CAGCCGTTCA GTACCAAGTC TCAGGGGAAA CTTTGAGATG GCCTTGCAAA
     2101 GGGTATGGTA ATAAGCTGAC GGACATGGTC CTAACCACGC AGCCAAGTCC TAAGTCAACA
     2161 GATCTTCTGT TGATATGGAT GCAGTTCACA AACTAAATGT CGGTCGGGGA AGATGTATTC
     2221 TTCTCATAAG ATATAGTCGG ACCTCTCCTT AATGGGAGCT AGCGGATGAA GTGATGCAAC
     2281 ACTGGAGCCG CTGGGAACTA ATTTGTATGC GAAAGTATAT TGATTAGTTT TGGAGTACTC
     2341 Gcttcatgct gcaacgaacg gaggttacac caacaccagg aaggagatct atgaagacgg
     2401 cggcatcttg gaggtcaact tccgttacac ttacgagttc aacaagatca tcggtgacgt
     2461 cgagtgcatt ggacatggat tcccaagtca gagtccgatc ttcaaggaca cgatcgtgaa
     2521 gagttgtccc acggtggacc tgatgttgcc aatgtccggg aacatcatcg ccagctccta
     2581 cgcttacgcc ttccaactga aggacggctc tttctacacg gcagaagtca agaacaacat
     2641 agacttcaag aatccaatcc acgagtcctt ctcgaagtca gggcccatgt tcacccacag
     2701 acgtgtcgag gagactctca ccaaggagaa ccttgccata gtggagtacc agcaggtttt
     2761 caacagcgcc ccaagagaca tgtagATATG AAGATGAAGA TGAAATATTT GGTGTGTCAA
     2821 ATAAAAAGCT TGTGTGCTTA AGTTTGTGTT TTTTTCTTGG CTTGTTGTGT TATGAATTTG
     2881 TGGCTTTTTC TAATATTAAA TGAATGTAAG ATCTCATTAT AATGAATAAA CAAATGTTTC
     2941 TATAATCCAT TGTGAATGTT TTGTTGGATC TCTTCTGCAG CATATAACTA CTGTATGTGC
     3001 TATGGTATGG ACTATGGAAT ATGATTAAAG ATAAGATGGG CTCATAGAGT AAAACGAGGC
     3061 GAGGGACCTA TAAACCTCCC TTCATCATGC TATTTCATGA TCTATTTTAT AAAATAAAGA
     3121 TGTAGAAAAA AGTAAGCGTA ATAACCGCAA AACAAATGAT TTAAAACATG GCACATAATG
     3181 AGGAGATTAA GTTCGGTTTA CGTTTATTTT AGTACTAATT GTAACGTGAG ACTACGTATC
     3241 GGGAATCGCC TAATTAAAGC ATTAATGCGA ACCTGATTAG ATTCACCGAC CCTCCTATCG
     3301 TGTCGACCTT TCTGTTTCTT AGAATTTTTT GGTAGTCTAT GTACTAATAA TGTCAGCTTC
     3361 GTATTTATTT CATAAGCAAT TTGCATTTGC AATTTGTTTT TTACTTTTAT TTTTATTGTA
     3421 TTGTGGAATG TGGACTCGTA CCAACATGAA GTTATATACC ACCAAAAAAA TTACAGTTAG
     3481 TCAAAAGATT CACGAGTGAG AGCTACTTAT GATTGTCTTT TACGTATATG TCTAATTGTC
     3541 TATTTGCTCA ATAATCTTTG TACTTTCTTT TGTCGTTGAT AAAATCACAA AGTTCCAAAA
     3601 GTAATCGAAT GATTTGCTTT TAAGAAAAGA AGAGCTCAAT AATTCAACAT ATATCTGTAC
     3661 ACAattaccg cctttgagtg agctctagat gccacctgac gtctaagaag aattcgtaat
     3721 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     3781 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3841 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3901 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc ttgccaacat
     3961 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
     4021 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
     4081 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
     4141 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
     4201 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4261 aaagcaagtg gattgatgtg ataacatggt ggagcacgac actctcgtct actccaagaa
     4321 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
     4381 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
     4441 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
     4501 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     4561 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
     4621 cgtaagggat gacgcacaat cccactatcc ttcgcaagac cttcctctat ataaggaagt
     4681 tcatttcatt tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc
     4741 tcgagctttc gcagatcccg gggggcaatg agatatgggg attgaacaag atggattgca
     4801 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4861 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     4921 tgtcaagacc gacctgtccg gtgccctgaa tgaactccag gacgaggcag cgcggctatc
     4981 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5041 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5101 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5161 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5221 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     5281 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgacaca
     5341 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5401 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5461 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     5521 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaagtagatg
     5581 ccgaccggat ctgtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc
     5641 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc
     5701 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     5761 caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt cagtacatta
     5821 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
     5881 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
     5941 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
     6001 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
     6061 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
     6121 tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa
     6181 caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt
     6241 ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta gaaaagagga
     6301 aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa
     6361 taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga
     6421 gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat
     6481 gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc
     6541 ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc
     6601 ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg
     6661 gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta
     6721 gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc
     6781 gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag
     6841 acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc
     6901 tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac
     6961 aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag
     7021 tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat
     7081 tatattttac tggatgaatt gttttagtac ctagaatgca tgaccaaaat cccttaacgt
     7141 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7201 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7261 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7321 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     7381 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7441 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7501 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7561 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7621 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7681 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7741 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7801 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7861 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7921 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     7981 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     8041 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     8101 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     8161 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     8221 caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt
     8281 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt
     8341 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac
     8401 cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag
     8461 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct
     8521 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg
     8581 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta
     8641 tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc
     8701 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg
     8761 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt
     8821 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg
     8881 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc
     8941 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc
     9001 agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc
     9061 tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct
     9121 tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca
     9181 acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca
     9241 tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc
     9301 ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc
     9361 agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg
     9421 tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga
     9481 cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc
     9541 ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg
     9601 gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca
     9661 ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc
     9721 tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg
     9781 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca
     9841 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
     9901 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
     9961 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
    10021 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
    10081 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
    10141 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
    10201 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
    10261 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
    10321 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
    10381 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
    10441 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
    10501 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
    10561 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
    10621 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
    10681 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
    10741 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
    10801 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
    10861 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
    10921 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
    10981 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
    11041 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
    11101 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
    11161 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
    11221 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
    11281 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
    11341 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
    11401 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
    11461 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
    11521 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
    11581 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
    11641 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
    11701 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
    11761 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
    11821 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
    11881 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
    11941 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat
    12001 agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
    12061 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgttattcta
    12121 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
    12181 aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
    12241 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
    12301 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
    12361 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
    12421 tgccaaatgt ttgaacgatc ggggaaattc gagctggtca ccaattcaca cgtgatggtg
    12481 atggtgatgg ctagcgttct tgtagccgaa atctggaatg ttggtccagc gctcgcgaaa
    12541 gacgtgcgcg gcgagcttcg gcttgcggtc acgagtgaac acgcccttct tgtttccttg
    12601 gacgcgcatc acgccctgag aggtcgcgaa gtccgcgaag ttccacgctt gctcacccac
    12661 gaagttctca aactcatcga acacgacgtg gttcgcctgg tagtactcga cttgatattc
    12721 ctcggtgaac atcactggat caatgtcgtg aaagcccgca acggtgtctg cgccgtactc
    12781 agtgatcatg atcggctttc ctgggcaacg cttgttccac gcgtgaaatt cctggcggag
    12841 atggactttg gccgcttcga gatcaccgcc atcgaagtac catccgttat agcgattgag
    12901 cgcgatgacg tcaatcagtt cggcgacttt gtccgtctcc ggggtagcca tcacaaacag
    12961 cacgatcgtg accggacgct tctgtgggtc gagttccttg gtcagctcca ccaacggctt
    13021 gaagtactcg tacgcgccct cttcctcagt cgccgcctcg ttggcgatgc tccacatcac
    13081 gacgcttgga tggttcttgt cacgagacac cagttcacgg agaacgtctt gatggtgctc
    13141 aaacgtccga atcttctccc aggtactgac gcgctcgctg ccttcgccga gtcccgtggt
    13201 ggccatgaag ttgaggtgca cgccaactgc cggagtctcg tcgatcacga ccagaccctc
    13261 gcgatccgca agacgcatca actcttcaga gtacggatag tgtgcggtcc ggaagctgtt
    13321 ggcgccgatc catttgagga tattgaaatc catcacattg ctcgcttcgt taaagccacg
    13381 gccgttgata ggagtgtcct catgtttgcc aaagcccttg aagtagaacg gtttgttgtt
    13441 gatgaggaac ttgccgtcgt tgacttccac ggtccgcacg ccgaacggct cttcatagac
    13501 atcgatggtc agtccgtcgt tcaccagttc cactttgatc tggtagagat acgtgttcag
    13561 tggttcccag aggatgacat tcggaatctc cacgttaccg ctcaggccct cggtgcttgc
    13621 gaccactttg ccttcctcat ccacgaccga cactttcacg gtctcggctt tgccttgaaa
    13681 gtccaccgta taggtcacag tcccggttgg gccattgaag tcggtcacaa ccgagatgtc
    13741 ctcgacgtac gtaaacgggg tcgtgtagat tttcaccgga cggtgcaggc ctgcatagtt
    13801 gaagaagtcg aagttcggct tgttacgaat gacttttccg aggccctctt cgtggcgctc
    13861 gctgtacagc cccaccggga gggtgctatc gtcgaggatg ttgtccacgg cgacggtgac
    13921 gcgattcatg ccatcacgca gcgagttgtt gatttccgct tcgaatggca ggaatccgcc
    13981 cttgtgctcc acgaccagct caccattgac atagacaatt gctttgtgag ttgcagagcc
    14041 gaagcggagc acgatacgct gatccttcag ataggccggc accgtgaact cacgttcgta
    14101 ccagacatat ccgatatggt tgcggatttc cttggtcacg ccaatgtcat tgtaactgct
    14161 tgggacggcc atactaatag tgtcggtcag cttgctttcg taccacttct cttccagtcc
    14221 tttcccgtag tccagcttga agttccagac gccattgagg tcgaagacgc cacgggtctc
    14281 ggtgttgatc gggtacagac tagttcgtcg gttctgtaac tatcatcatc atcatagaca
    14341 cacgaaataa agtaatcaga ttatcagtta aagctatgta atatttacac cataaccaat
    14401 caattaaaaa atagatcagt ttaaagaaag atcaaagctc aaaaaaataa aaagagaaaa
    14461 gggtcctaac caagaaaatg aaggagaaaa actagaaatt taccctcaga tctaccatg
//