LOCUS       BAC_gws1b_stock         9036 bp    DNA     circular SYN 08-MAY-2025
DEFINITION  BAC_gws1b_stock.
ACCESSION   BAC_gws1b_stock
VERSION     BAC_gws1b_stock
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     misc_feature    complement(5738..6211)
                     /note="pLannotate"
                     /label="sopC"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     misc_feature    complement(8756..9006)
                     /note="pLannotate"
                     /label="incC"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      complement(819..1038)
                     /note="pLannotate"
                     /label="ori2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             4210..5413
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="99.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(7255..8430)
                     /note="pLannotate"
                     /label="sopA"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(2125..2229)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(6284..7255)
                     /note="pLannotate"
                     /label="sopB"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1264..2124)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(join(9009..9036,1..728))
                     /note="pLannotate"
                     /label="repE"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2430..3806
                     /note="pLannotate"
                     /label="cbbM"
                     /database="swissprot"
                     /identity="87.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        2363..2391
                     /note="pLannotate"
                     /label="tac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4137..4165
                     /note="pLannotate"
                     /label="tet promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        2338..2356
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             3807..3824
                     /note="pLannotate"
                     /label="9xHis (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="66.7"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(5525..5638)
                     /note="pLannotate"
                     /label="yuaZ (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="42.2"
                     /fragment="True"
                     /other="CDS"
     CDS             3840..3857
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="10.3"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 cggaaggaaa atacgatatg agtcgtctgg cggcctttct ttttctcaat gtatgagagg
       61 cgcattggag ttctgctgtt gatctcatta acacagacct gcaggaagcg gcggcggaag
      121 tcaggcatac gctggtaact ttgaggcagc tggtaacgct ctatgatcca gtcgattttc
      181 agagagacga tgcctgagcc atccggctta cgatactgac acagggattc gtataaacgc
      241 atggcatacg gattggtgat ttcttttgtt tcactaagcc gaaactgcgt aaaccggttc
      301 tgtaacccga taaagaaggg aatgagatat gggttgatat gtacactgta aagccctctg
      361 gatggactgt gcgcacgttt gataaaccaa ggaaaagatt catagcctat ttcatcgccg
      421 gcatcctctt cagggcgata aaaaaccact tccttccccg cgaaactctt caatgcctgc
      481 cgtatatcct tactggcttc cgcagaggtc aatccgaata tttcagcata tttagcaaca
      541 tggatctcgc agataccgtc atgttcctgt agggtgccat cagattttct gatctggtca
      601 acgaacagat acagcatacg tttttgatcc cgggagagac tatatgccgc ctcagtgagg
      661 tcgtttgact ggacgattcg cgggctattt ttacgtttct tgtgattgat aaccgctgtt
      721 tccgccatga cagatccatg tgaagtgtga caagttttta gattgtcaca ctaaataaaa
      781 aagagtcaat aagcagggat aactttgtga aaaaacagct tcttctgagg gcaatttgtc
      841 acagggttaa gggcaatttg tcacagacag gactgtcatt tgagggtgat ttgtcacact
      901 gaaagggcaa tttgtcacaa caccttctct agaaccagca tggataaagg cctacaaggc
      961 gctctaaaaa agaagatcta aaaactataa aaaaaataat tataaaaata tccccgtgga
     1021 taagtggata accccaaggg aagttttttc aggcatcgtg tgtaagcaga atatataagt
     1081 gctgttccct ggtgcttcct cgctcactcg agctacgggg tctgacgctc agtggaacga
     1141 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1201 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1261 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1321 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     1381 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     1441 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     1501 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     1561 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     1621 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     1681 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     1741 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     1801 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     1861 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     1921 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     1981 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2041 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2101 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2161 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2221 ggttccgcgc acatttcccc gaaaagtgcc acctggcggc cgcctttcag caaaaaaccc
     2281 cgcgagaccc ccgaagaggc cccgcggggt tatgctaggt cgacggagct cgaattctaa
     2341 tacgactcac tatagggaga ccttgacaat taatcatcgg ctcgtataat gccagaagct
     2401 tagcaaaagc taaaaccagg agctatttaa tggaccaaag taatcgttac gccgacttat
     2461 ccttgaaaga ggaggacttg atcaaggggg ggaatcatat cctggttgcc tatacaatgg
     2521 aacccgctgc cggggtcgga tatcttgaag cagccgccca cattgccgca gagagttcta
     2581 ctggaaccaa tgtggaggtc tcgactacgg atgaatttac aaaaggggtc gatgctcttg
     2641 tgtatttcat tgatgaggcc aagggcatca tgaaggtcgc gtatcctaat gatctgttcg
     2701 atcgcaatgt taccgatggg cgcgtgatga tcgtttcctt cttgaccctt tgtattggaa
     2761 ataatcaagg aatgggagac attgcaaatc tgcagatgca ggacttctac gttcccccgc
     2821 gtatgttgca actgtttgac gggcctgcga aggatatttc agatttatgg cgcatcttgg
     2881 gtcgtccagt taaagatggt gggtacatct caggaacaat tattaaaccg aaattaggtc
     2941 ttcgtcccga acctttcgct aaggcagcct atcaattttg gcttggcggt gattttatca
     3001 agaatgatga gccccaaggt aaccaggtat tttgcccaat caagaaagtc ctgcccttgg
     3061 tttacgattc gttaaagcgc gcccaggacg aaactgggca agccaaattg tttagtatga
     3121 acatcacggc agatgatcat tacgagatgt gcgcgcgcgc tgactttgcc cttgaaacct
     3181 tcgggccgga tgctgacaaa gtggcctttc ttgttgacgg ttttgtcggt gggccgggga
     3241 tggttacaac agcccgtcgc caatatccct cccaatactt gcactaccac cgtgcaggtc
     3301 acggcatggt gactagccca tcctctaagc gcggatacac ggcctttgtg ctggcgaaga
     3361 tgagtcgttt acaaggggca tccgggatcc atgttggcac tatgggatac ggaaaaatgg
     3421 aaggcggcaa agacgatcgc attattgcct acatgatcga gcgtgatagc tgcacgggac
     3481 cattctatca tcaagagtgg tatggtatga agcctacaac tccaattatt tctggaggta
     3541 tgaacgcttt gcgtttgcct ggcttcttcg agaatctggg acacggaaac gtaattaaca
     3601 ctgctggcgg tggaagctac ggccatatcg actcgccagc ggcaggagct gtctcgttac
     3661 gccaggccta tgaatgttgg aaagctgggg ccgatcctat cgagtgggct aaggagcaca
     3721 aggaattcgc gcgtgcgttt gaatcctttc caaaagacgc ggacaaattg ttccccggat
     3781 ggcgcgaaaa attaggagta cataaacacc accaccacca ccactagtgg tttaattaac
     3841 gcagcctgaa tggcgaatag aagtttaaac gctagaccta gcataacccc gcggggcctc
     3901 ttcgggggtc tcgcggggtt ttttgctgaa aggctagacc tagcataacc ccgcggggcc
     3961 tcttcggggg tctcgcgggg ttttttgctg aaaggctaga cctagcataa ccccgcgggg
     4021 cctcttcggg ggtctcgcgg ggttttttgc tgaaaggcta gacctagcat aaccccgcgg
     4081 ggcctcttcg ggggtctcgc ggggtttttt gctgaaaggc taggatatat tgatccttga
     4141 cagcttatca tcgataagct ttaatgcggt agtttatcac agttgctaac gcagtcaggc
     4201 accgtgtacg aatagttcga caaagatcgc attggtaatt acgttactcg atgccatggg
     4261 gattggcctt atcatgccag tcttgccaac gttattacgt gaatttattg cttcggaaga
     4321 tatcgctaac cactttggcg tattgcttgc actttatgcg ttaatgcagg ttatctttgc
     4381 tccttggctt ggaaaaatgt ctgaccgatt tggtcggcgc ccagtgctgt tgttgtcatt
     4441 aataggcgca tcgctggatt acttattgct ggctttttca agtgcgcttt ggatgctgta
     4501 tttaggccgt ttgctttcag ggatcacagg agctactggg gctgtcgcgg catcggtcat
     4561 tgccgatacc acctcagctt ctcaacgcgt gaagtggttc ggttggttag gggcaagttt
     4621 tgggcttggt ttaatagcgg ggcctattat tggtggtttt gcaggagaga tttcaccgca
     4681 tagtcccttt tttatcgctg cgttgctaaa tattgtcact ttccttgtgg ttatgttttg
     4741 gttccgtgaa accaaaaata cacgtgataa tacagatacc gaagtagggg ttgagacgca
     4801 atcgaattcg gtatacatca ctttatttaa aacgatgccc attttgttga ttatttattt
     4861 ttcagcgcaa ttgataggcc aaattcccgc aacggtgtgg gtgctattta ccgaaaatcg
     4921 ttttggatgg aatagcatga tggttggctt ttcattagcg ggtcttggtc ttttacactc
     4981 agtattccaa gcctttgtgg caggaagaat agccactaaa tggggcgaaa aaacggcagt
     5041 actgctcgga tttattgcag atagtagtgc atttgccttt ttagcgttta tatctgaagg
     5101 ttggttagtt ttccctgttt taattttatt ggctggtggt gggatcgctt tacctgcatt
     5161 acagggagtg atgtctatcc aaacaaagag tcatcagcaa ggtgctttac agggattatt
     5221 ggtgagcctt accaatgcaa ccggtgttat tggcccatta ctgtttgctg ttatttataa
     5281 tcattcacta ccaatttggg atggctggat ttggattatt ggtttagcgt tttactgtat
     5341 tattatcctg ctatcgatga ccttcatgtt aacccctcaa gctcagggga gtaaacagga
     5401 gacaagtgct tagtgatcca attcttgaag acgaaagggc ctcgtgatac gcctattttt
     5461 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5521 tgtgcccggt taacgtgccg gcacggcctg ggtaaccagg tattttgtcc acataaccgt
     5581 gcgcaaaatg ttgtggataa gcaggacaca gcagcaatcc acagcaggca tacaaccgca
     5641 caccgaggtt actccgttct acaggttacg acgacatgtc aatacttgcc cttgacaggc
     5701 attgatggaa tcgtagtctc acgctgatag tctgatcgac aatacaagtg ggaccgtggt
     5761 cccagaccga taatcagacc gacaacacga gtgggatcgt ggtcccagac taataatcag
     5821 accgacgata cgagtgggac cgtggtccca gactaataat cagaccgacg atacgagtgg
     5881 gaccgtggtt ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagact
     5941 aataatcaga ccgacgatac gagtgggacc atggtcccag actaataatc agaccgacga
     6001 tacgagtggg accgtggtcc cagtctgatt atcagaccga cgatacgagt gggaccgtgg
     6061 tcccagacta ataatcagac cgacgatacg agtgggaccg tggtcccaga ctaataatca
     6121 gaccgacgat acgagtggga ccgtggtccc agtctgatta tcagaccgac gatacaagtg
     6181 gaacagtggg cccagagaga atattcaggc cagttatgct ttctggcctg taacaaagga
     6241 cattaagtaa agacagataa acgtagacta aaacgtggtc gcatcagggt gctggctttt
     6301 caagttcctt aagaatggcc tcaattttct ctatacactc agttggaaca cgagacctgt
     6361 ccaggttaag caccatttta tcgcccttat acaatactgt cgctccagga gcaaactgat
     6421 gtcgtgagct taaactagtt cttgatgcag atgacgtttt aagcacagaa gttaaaagag
     6481 tgataacttc ttcagcttca aatatcaccc cagctttttt ctgctcatga aggttagatg
     6541 cctgctgctt aagtaattcc tctttatctg caaaggcttt ttgaagtgca tcacctgacc
     6601 gggcagatag ttcaccgggg tgagaaaaaa gagcaacaac tgatttaggc aatttggcgg
     6661 tgttgataca gcgggtaata atcttacgtg aaatattttc cgcatcagcc agcgcagaaa
     6721 tatttccagc aaattcattc tgcaatcggc ttgcataacg ctgaccacgt tcataagcac
     6781 ttgttgggcg ataatcgtta cccaatctgg ataatgcagc catctgctca tcatccagct
     6841 cgccaaccag aacacgataa tcactttcgg taagtgcagc agctttacga cggcgactcc
     6901 catcggcaat ttctatgaca ccagatactc ttcgaccgaa cgccggtgtc tgttgaccag
     6961 tcagtagaaa agaagggatg agatcatcca gtgcgtcctc agtaagcagc tcctggtcac
     7021 gttcattacc tgaccatacc cgagaggtct tctcaacact atcaccccgg agcacttcaa
     7081 gagtaaactt cacatcccga ccacatacag gcaaagtaat ggcattaccg cgagccatta
     7141 ctcctacgcg cgcaattaac gaatccacca tcggggcagc tggtgtcgat aacgaagtat
     7201 cttcaaccgg ttgagtattg agcgtatgtt ttggaataac aggcgcacgc ttcattatct
     7261 aatctcccag cgtggtttaa tcagacgatc gaaaatttca ttgcagacag gttcccaaat
     7321 agaaagagca tttctccagg caccagttga agagcgttga tcaatggcct gttcaaaaac
     7381 agttctcatc cggatcggac ctttaccaac ttcatccgtt tcacgtacaa cattttttag
     7441 aaccatgctt ccccaggcat cccgaatttg ctcctccatc cacggggact gagagccatt
     7501 actattgctg tatttggtaa gcaaaatacg tacatcaggc tcgaaccctt taagatcaac
     7561 gttcttgagc agatcacgaa gcatatcgaa aaactgcagt gcggaggtgt agtcaaacaa
     7621 ctcagcaggc gtgggaacaa tcagcacatc agcagcacat acgacattaa tcgtgccgat
     7681 acccaggtta ggcgcgctgt caataactat gacatcatag tcatgagcaa cagtttcaat
     7741 ggccagtcgg agcatcaggt gtggatcggt gggcagttta ccttcatcaa atttgcccat
     7801 taactcagtt tcaatacggt gcagagccag acaggaagga ataatgtcaa gccccggcca
     7861 gcaagtgggc tttattgcat aagtgacatc gtccttttcc ccaagataga aaggcaggag
     7921 agtgtcttct gcatgaatat gaagatctgg tacccatccg tgatacattg aggctgttcc
     7981 ctgggggtcg ttaccttcca cgagcaaaac acgtagcccc ttcagagcca gatcctgagc
     8041 aagatgaaca gaaactgagg ttttgtaaac gccaccttta tgggcagcaa ccccgatcac
     8101 cggtggaaat acgtcttcag cacgtcgcaa tcgcgtacca aacacatcac gcatatgatt
     8161 aatttgttca attgtataac caacacgttg ctcaacccgt cctcgaattt ccatatccgg
     8221 gtgcggtagt cgccctgctt tctcggcatc tctgatagcc tgagaagaaa ccccaactaa
     8281 atccgctgct tcacctattc tccagcgccg ggttattttc ctcgcttccg ggctgtcatc
     8341 attaaactgt gcaatggcga tagccttcgt catttcatga ccagcgttta tgcactggtt
     8401 aagtgtttcc atgagtttca ttctgaacat cctttaatca ttgctttgcg tttttttatt
     8461 aaatcttgca atttactgca aagcaacaac aaaatcgcaa agtcatcaaa aaaccgcaaa
     8521 gttgtttaaa ataagagcaa cactacaaaa ggagataaga agagcacata cctcagtcac
     8581 ttattatcac tagcgctcgc cgcagccgtg taaccgagca tagcgagcga actggcgagg
     8641 aagcaaagaa gaactgttct gtcagatagc tcttacgctc agcgcaagaa gaaatatcca
     8701 ccgtgggaaa aactccaggt agaggtacac acgcggatag ccaattcaga gtaataaact
     8761 gtgataatca accctcatca atgatgacga actaaccccc gatatcaggt cacatgacga
     8821 agggaaagag aaggaaatca actgtgacaa actgccctca aatttggctt ccttaaaaat
     8881 tacagttcaa aaagtatgag aaaatccatg caggctgaag gaaacagcaa aactgtgaca
     8941 aattaccctc agtaggtcag aacaaatgtg acgaaccacc ctcaaatctg tgacagataa
     9001 ccctcagact atcctgtcgt catggaagtg atatcg
//
