LOCUS       pRRL.TRE3G.ZIM3.Cas9.P2A.GFP       13752 bp    DNA     circular UNA 05-MAY-2025
DEFINITION  Generated by Gibson/Homology Cloning (JJ9_pRRL.TRE3G.Cas9.P2A.GFP
            (digested by AfeI), insert_1 PCR Product).
ACCESSION   urn.local...2q-d7tr3bn
VERSION     urn.local...2q-d7tr3bn
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     polyA_signal    join(13624..13752,1..96)
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      142..570
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        584..913
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     rep_origin      764..899
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        961..1008
                     /note="synthetic bacterial promoter"
                     /label="EM7 promoter"
     CDS             1027..1401
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
                     /label="BleoR"
     polyA_signal    1531..1652
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(1701..1717)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 rev"
     protein_bind    1725..1741
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(1749..1779)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    1794..1815
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the
                     presenceof cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2103..2691)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     CDS             complement(2862..3722)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     promoter        complement(3723..3827)
                     /gene="bla"
                     /label="AmpR promoter"
     enhancer        4093..4472
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        4473..4676
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     LTR             4690..4870
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    4917..5042
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /label="HIV-1 Psi"
     misc_feature    5535..5768
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to
                     thecytoplasm."
                     /label="RRE"
     misc_feature    6295..6412
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        6476..6854
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On(R) 3G"
                     /label="TRE3G promoter"
     protein_bind    6484..6502
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    6520..6538
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    6556..6574
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    6592..6610
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    6628..6646
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    6664..6682
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    6700..6718
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     Fragment        6861..7317
                     /Source_Sequence="referenceToDocument:
                     urn:local:.:s-d68noe9@22.0#0#C4A1CB1C5BCD804C0B0BE036B8524
                     B49 label: insert_1 PCR Product"
                     /Cloning_Method="Gibson Cloning"
                     /Fragment_Type="Insert"
                     /label="insert_1 PCR Product (insert)"
     ligation        6861..6880
                     /label="Ligation"
     primer_bind     6861..6911
                     /Sequence="CTTGCGTTGGATCCACCAGCATGAACAATTCCCAGGGAAGAGTGACC
                     TTCG"
                     /Tm="71.7"
                     /%GC="52.9"
                     /Hairpin_Tm="46.6"
                     /Self_Dimer_Tm="23.8"
                     /created_by="julianjude"
                     /label="ZIM3-KRAB_fwd"
     CDS             6881..7294
                     /created_by="julianjude"
                     /X-Transferred_From="JJ9_pRRL.TRE3G.Cas9.P2A.GFP Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; a (<a
                     href=""urn:local:.:dq-czk4k8o"">JJ9_pRRL.TRE3G.Cas9.P2A.GF
                     P Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; a
                     (<a
                     href=""urn:local:.:t-czjzskw"">pLX303-ZIM3-KRAB-dCas9</a>)
                     "
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:345/-48; SV40 NLS (<a
                     href=""urn:local:.:13-czk3jmu"">Exported extraction</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:345/-48; SV40 NLS (<a
                     href=""urn:local:.:dq-czk4k8o"">JJ9_pRRL.TRE3G.Cas9.P2A.GF
                     P Copy</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:345/-48; SV40 NLS (<a
                     href=""urn:local:.:y-czk02li"">Exported</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:345/-48; SV40 NLS (<a
                     href=""urn:local:.:t-czjzskw"">pLX303-ZIM3-KRAB-dCas9</a>)
                     "
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:345/-48; SV40 NLS (<a
                     href=""urn:local:.:1k-czk3n6r"">Exported extraction
                     extraction</a>)"
                     /note="Derived using Geneious Prime 2021.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Clipboard Contents"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; a (<a
                     href=""urn:local:.:ed-czk5uam"">Clipboard Contents</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; a
                     (<a
                     href=""urn:local:.:dq-czk4k8o"">JJ9_pRRL.TRE3G.Cas9.P2A.GF
                     P Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; a
                     (<a
                     href=""urn:local:.:t-czjzskw"">pLX303-ZIM3-KRAB-dCas9</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; a
                     (<a href=""urn:local:.:ev-czk6rw7"">Clipboard Contents
                     2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:345/-48;
                     SV40 NLS (<a href=""urn:local:.:13-czk3jmu"">Exported
                     extraction</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:345/-48;
                     SV40 NLS (<a
                     href=""urn:local:.:dq-czk4k8o"">JJ9_pRRL.TRE3G.Cas9.P2A.GF
                     P Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:345/-48;
                     SV40 NLS (<a href=""urn:local:.:y-czk02li"">Exported</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:345/-48;
                     SV40 NLS (<a
                     href=""urn:local:.:t-czjzskw"">pLX303-ZIM3-KRAB-dCas9</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:345/-48;
                     SV40 NLS (<a href=""urn:local:.:1k-czk3n6r"">Exported
                     extraction extraction</a>)"
                     /modified_by="Biochem_Jannis"
                     /label="ZIM3"
     protein_bind    complement(7182..7206)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="Derived using Geneious Prime 2021.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Exported extraction"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB2
                     (<a href=""urn:local:.:13-czk3jmu"">Exported
                     extraction</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a href=""urn:local:.:ed-czk5uam"">Clipboard
                     Contents</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a
                     href=""urn:local:.:dq-czk4k8o"">JJ9_pRRL.TRE3G.Cas9.P2A.GF
                     P Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a href=""urn:local:.:y-czk02li"">Exported</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a
                     href=""urn:local:.:t-czjzskw"">pLX303-ZIM3-KRAB-dCas9</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a href=""urn:local:.:1k-czk3n6r"">Exported
                     extraction extraction</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a href=""urn:local:.:ev-czk6rw7"">Clipboard
                     Contents 2</a>)"
                     /label="attB2"
     primer_bind     complement(7277..7317)
                     /Sequence="TTCTTGTCCATGGTGGCAGCGCTGATCCCGGGCCCGCGGTA"
                     /Tm="76.1"
                     /%GC="65.9"
                     /Hairpin_Tm="36.6"
                     /Self_Dimer_Tm="30.2"
                     /created_by="julianjude"
                     /label="ZIM3-KRAB_rev"
     Fragment        7298..6880
                     /Source_Sequence="referenceToDocument:
                     urn:local:.:1v-d5ndhwu@31.1#0#41F30D21067ED2BE7C284F490733
                     FAC0 label: JJ9_pRRL.TRE3G.Cas9.P2A.GFP (digested by
                     AfeI)"
                     /Cloning_Method="Gibson Cloning"
                     /Fragment_Type="Backbone"
                     /label="JJ9_pRRL.TRE3G.Cas9.P2A.GFP (digested by AfeI)
                     (backbone)"
     ligation        7298..7317
                     /label="Ligation"
     regulatory      7301..7310
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong
                     initiationof translation (Kozak, 1987)"
                     /note="Derived using Geneious Prime 2021.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Exported extraction"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     regulatory (<a href=""urn:local:.:13-czk3jmu"">Exported
                     extraction</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     regulatory (<a href=""urn:local:.:ed-czk5uam"">Clipboard
                     Contents</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     regulatory (<a
                     href=""urn:local:.:dq-czk4k8o"">JJ9_pRRL.TRE3G.Cas9.P2A.GF
                     P Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     regulatory (<a
                     href=""urn:local:.:h-czjydc8"">JJ9_pRRL.TRE3G.Cas9.P2A.GFP
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     regulatory (<a
                     href=""urn:local:.:y-czk02li"">Exported</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     regulatory (<a href=""urn:local:.:ev-czk6rw7"">Clipboard
                     Contents 2</a>)"
                     /label="regulatory"
     regulatory      7301..7310
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong
                     initiationof translation (Kozak, 1987)"
                     /label="regulatory"
     CDS             7307..11410
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /label="Cas9"
     CDS             7307..>7317
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /X-Transferred_Translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSK
                     KFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSN
                     EMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDST
                     DKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINAS
                     GVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDA
                     KLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSAS
                     MIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKP
                     ILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDN
                     REKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIER
                     MTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLL
                     FKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNE
                     ENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLIN
                     GIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIAN
                     LAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRI
                     EEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIV
                     PQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNL
                     TKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITL
                     KSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVY
                     DVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVW
                     DKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYG
                     GFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEV
                     KKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGS
                     PEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQA
                     ENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQ
                     LGGD"
                     /X-Transferred_From="JJ9_pRRL.TRE3G.Cas9.P2A.GFP Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Cas9
                     (<a
                     href=""urn:local:.:dq-czk4k8o"">JJ9_pRRL.TRE3G.Cas9.P2A.GF
                     P Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Cas9 (<a
                     href=""urn:local:.:h-czjydc8"">JJ9_pRRL.TRE3G.Cas9.P2A.GFP
                     </a>)"
                     /X-Alternative_Match="99.05%; Adjusted-Interval-0:0/0;
                     dCas9 (<a href=""urn:local:.:13-czk3jmu"">Exported
                     extraction</a>)"
                     /X-Alternative_Match="99.05%; Adjusted-Interval-0:0/0;
                     dCas9 (<a href=""urn:local:.:y-czk02li"">Exported</a>)"
                     /note="Derived using Geneious Prime 2021.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Clipboard Contents"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Cas9
                     (<ahref=""urn:local:.:ed-czk5uam"">Clipboard
                     Contents</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Cas9(<ahref=""urn:local:.:dq-czk4k8o"">JJ9_pRRL.TRE3G.Cas9
                     .P2A.GFP Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Cas9(<ahref=""urn:local:.:h-czjydc8"">JJ9_pRRL.TRE3G.Cas9.
                     P2A.GFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Cas9(<a href=""urn:local:.:ev-czk6rw7"">Clipboard
                     Contents2</a>)"
                     /label="Cas9"
     CDS             11411..11458
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /translation="KRPAATKKAGQAKKKK"
                     /label="nucleoplasmin NLS"
     CDS             11459..11482
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an
                     enterokinasecleavage site"
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             11498..11554
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding
                     separatepolypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             11555..12274
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     misc_feature    12299..12887
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /label="WPRE"
     CDS             complement(12770..12781)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
                     /label="Factor Xa site"
     primer_bind     complement(12890..12906)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="KS primer"
     LTR             13412..13592
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
ORIGIN      
        1 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
       61 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
      121 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      181 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      241 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      301 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
      361 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
      421 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
      481 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
      541 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
      601 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
      661 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      721 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
      781 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
      841 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
      901 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
      961 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     1021 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     1081 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     1141 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     1201 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     1261 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     1321 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     1381 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
     1441 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     1501 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     1561 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     1621 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     1681 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     1741 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     1801 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     1861 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1921 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1981 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2041 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2101 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2161 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2221 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2281 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2341 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2401 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2461 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2521 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2581 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2641 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2701 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2761 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2821 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2881 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2941 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3001 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3061 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3121 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3181 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3241 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3301 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3361 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3421 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3481 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3541 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3601 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3661 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3721 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3781 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3841 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     3901 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     3961 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     4021 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     4081 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     4141 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     4201 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     4261 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     4321 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     4381 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     4441 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     4501 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     4561 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     4621 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt
     4681 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4741 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4801 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4861 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     4921 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     4981 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     5041 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     5101 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     5161 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     5221 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     5281 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     5341 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     5401 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     5461 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     5521 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     5581 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     5641 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     5701 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     5761 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     5821 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     5881 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     5941 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     6001 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     6061 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     6121 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     6181 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     6241 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     6301 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     6361 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     6421 ttacagggac agcagagatc cagtttggtt aattagctag cgatcctcta gattcgagtt
     6481 tactccctat cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac
     6541 gtatgcagac tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca
     6601 gtgatagaga acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt
     6661 tactccctat cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac
     6721 gtataagctt taggcgtgta cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg
     6781 tcagatcgcc tggagcaatt ccacaacact tttgtcttat accaactttc cgtaccactt
     6841 cctaccctcg taaagtcgag cttgcgttgg atccaccagc atgaacaatt cccagggaag
     6901 agtgaccttc gaggatgtca ctgtgaactt cacccagggg gagtggcagc ggctgaatcc
     6961 cgaacagaga aacttgtaca gggatgtgat gctggagaat tacagcaacc ttgtctctgt
     7021 gggacaaggg gaaaccacca aacccgatgt gatcttgagg ttggaacaag gaaaggagcc
     7081 atggttggag gaagaggaag tgctgggaag tggccgtgca gaaaaaaatg gggacattgg
     7141 agggcagatt tggaagccaa aggatgtgaa agagagtctc tacccaactt tcttgtacaa
     7201 agtggttggt ggatctggag gttctcccaa gaagaagcgc aaggtgggac gcgtctgcag
     7261 gatatcaagc ttgcggtacc gcgggcccgg gatcagcgct gccaccatgg acaagaagta
     7321 cagcatcggc ctggacatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta
     7381 caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa
     7441 gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa
     7501 gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat
     7561 cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt
     7621 cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga
     7681 ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag
     7741 caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg
     7801 gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt
     7861 catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg
     7921 cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct
     7981 gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag
     8041 cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca
     8101 gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca
     8161 gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat
     8221 cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata
     8281 cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga
     8341 gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg
     8401 cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg
     8461 caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt
     8521 cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg
     8581 gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac
     8641 cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat
     8701 gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg
     8761 cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga
     8821 gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac
     8881 caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa
     8941 aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa
     9001 agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga
     9061 tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga
     9121 cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact
     9181 gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga
     9241 caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa
     9301 gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc
     9361 cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa
     9421 agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc
     9481 caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga
     9541 cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag
     9601 agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga
     9661 agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca
     9721 gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga
     9781 ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag
     9841 ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg
     9901 caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca
     9961 gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag
    10021 aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg
    10081 gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga
    10141 gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga
    10201 tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca
    10261 cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga
    10321 aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag
    10381 cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt
    10441 tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac
    10501 aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa
    10561 agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt
    10621 cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga
    10681 ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt
    10741 ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctactggg
    10801 gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa
    10861 gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga
    10921 gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga
    10981 actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct
    11041 gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta
    11101 cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc
    11161 taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca
    11221 ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa
    11281 gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc
    11341 caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct
    11401 gggaggcgac aagcgacctg ccgccacaaa gaaggctgga caggctaaga agaagaaaga
    11461 ttacaaagac gatgacgata agggatccgg cagcggcgcc accaacttca gcctgctgaa
    11521 gcaggccggc gacgtggagg agaaccccgg ccccatggtg agcaagggcg aggagctgtt
    11581 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
    11641 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
    11701 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
    11761 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
    11821 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
    11881 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
    11941 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
    12001 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
    12061 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
    12121 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
    12181 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
    12241 gatcactctc ggcatggacg agctgtacaa gtaagaattc gatatcaagc ttatcggtaa
    12301 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
    12361 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
    12421 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
    12481 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
    12541 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
    12601 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
    12661 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
    12721 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
    12781 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
    12841 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
    12901 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
    12961 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
    13021 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
    13081 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
    13141 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
    13201 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
    13261 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
    13321 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
    13381 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
    13441 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    13501 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    13561 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
    13621 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
    13681 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
    13741 gtctgagtag gt
//
