LOCUS       pXYB107_dRibozyme_spli 14547 bp ds-DNA     circular     24-SEP-2023
DEFINITION  .
KEYWORDS    "creator:Xiaohan Yang" "marker:NeoR/KanR, KanR, Modified
            COR47-5'-UTR"
COMMENT     pXYB031 difested by PacI and SbfI+gYL091
FEATURES             Location/Qualifiers
     promoter        complement(16..361)
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     primer_bind     738..754
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     primer          738..754
                     /label="oYL_ORNL048"
                     /note="sequence: gtaaaacgacggccagt"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    745..774
                     /label="gibson overhang"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    745..1756
                     /label="gYL091"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(773..790)
                     /label="oYL_ORNL_047"
                     /note="sequence: ggctaatctggggacctg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        1269..1614
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     misc_feature    1599..1614
                     /label="overlap1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1615..1721
                     /label="Modified COR47-5'-UTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1697..1726
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1697..3360
                     /label="gYL094"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1727..1756
                     /label="gibson overhang"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1730..1754
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1730..1945
                     /label="1st half GFPuv"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1730..2776
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MTTFKIESRIHGNLNGEKFELVGGGVGEEGRLEIEMKTKDKPLAFSPFLLTTCMGYGFYHFASFPKGIKNIYKSYQACTW*LVFKPIDCIGLKGKTVKLRERGQQPFSTKSQGKL*DGLAKGMVIS*RTWS*PRSQVLSQQIFC*YGCSSQTKCRSGKMYSSHKI*SDLSLMGASG*SDATLEPLGTNLYAKVY*LVLEYSLHAATNGGYTNTRKEIYEDGGILEVNFRYTYEFNKIIGDVECIGHGFPSQSPIFKDTIVKSCPTVDLMLPMSGNIIASSYAYAFQLKDGSFYTAEVKNNIDFKNPIHESFSKSGPMFTHRRVEETLTKENLAIVEYQQVFNSAPRDM*"
     misc_feature    1760..1784
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1946..2332
                     /label="dRIbozyme"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2333..2776
                     /label="2nd half GFPuv"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2677..2701
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2777..2813
                     /label="tevopreq_motif"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2814..3691
                     /label="HSPT878"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2839..2863
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3325..3360
                     /label="Gibson overhang"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3692..3712
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3743..3767
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(3749..3769)
                     /label="oYL_ORNL029"
                     /note="sequence: caggaaacagctatgaccatg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(3753..3769)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    3777..3793
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3801..3807)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3808..3825)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3826..3831)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    3846..3867
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        4058..4735
                     /label="CaMV 35S promoter (enhanced)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: cauliflower mosaic virus 35S promoter with a duplicated enhancer region"
     misc_feature    4739..5665
                     /label="gBlocks for change to Kan"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4803..5600
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-II (or nptII) note: confers resistance to neomycin, kanamycin, and G418 (Geneticin®) product: aminoglycoside phosphotransferase from Tn5 transl_table: 1 translation: MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     polyA_signal    5640..5814
                     /label="CaMV poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: cauliflower mosaic virus polyadenylation signal"
     misc_feature    5892..5916
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: left border repeat from nopaline C58 T-DNA"
     CDS             6341..7135
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aphA-3 note: confers resistance to kanamycin product: aminoglycoside phosphotransferase protein_id: transl_table: 1 translation: MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"
     rep_origin      7222..7810
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     misc_feature    7996..8136
                     /label="bom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: basis of mobility region from pBR322"
     rep_origin      8480..8674
                     /label="pVS1 oriV"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)"
     CDS             complement(8740..9807)
                     /label="pVS1 RepA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 note: product: replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) protein_id: transl_table: 1 translation: GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     CDS             complement(10241..10870)
                     /label="pVS1 StaA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 product: stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) transl_table: 1 translation: MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
     misc_feature    12170..12194
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: right border repeat from nopaline C58 T-DNA"
     terminator      12216..12468
                     /label="NOS terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: nopaline synthase terminator and poly(A) signal"
     primer          12439..12465
                     /label="oYL072_oNB395 copy"
                     /note="sequence: GAAACTTTATTGCCAAATGTTTGAACG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(12500..12517)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: 6xHis affinity tag transl_table: 1 translation: HHHHHH"
     CDS             complement(12524..14341)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14342..14531
                     /label="cat1 intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: castor bean catalase intron, modified"
     CDS             complement(14342..14531)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     CDS             complement(14532..14546)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacaCAAACA
     1621 TTACTCATTC ACAAAACCAT CTTAAAGCAA CTACACAAGT CTTGAAATTT TCTCATATTT
     1681 TCTATTTACT ATATAAACTT TTAATCAAAT CAAGATTAAA GTTAATTAAa tgacaacctt
     1741 caaaatcgag tcccggatcc acggcaacct caacggggag aagttcgagt tggttggagg
     1801 tggagtaggt gaggagggtc gcctcgagat tgagatgaag actaaagata aaccactggc
     1861 attctctccc ttcctgctga ccacttgcat gggttacggg ttctaccact tcgccagctt
     1921 cccaaagggg attaagaaca tctatAAAAG TTATCAGGCA TGCACCTGGT AGCTAGTCTT
     1981 TAAACCAATA GATTGCATCG GTTTAAAAGG CAAGACCGTC AAATTGCGGG AAAGGGGTCA
     2041 ACAGCCGTTC AGTACCAAGT CTCAGGGGAA ACTTTGAGAT GGCCTTGCAA AGGGTATGGT
     2101 AATAAGCTGA CGGACATGGT CCTAACCACG CAGCCAAGTC CTAAGTCAAC AGATCTTCTG
     2161 TTGATATGGA TGCAGTTCAC AAACTAAATG TCGGTCGGGG AAGATGTATT CTTCTCATAA
     2221 GATATAGTCG GACCTCTCCT TAATGGGAGC TAGCGGATGA AGTGATGCAA CACTGGAGCC
     2281 GCTGGGAACT AATTTGTATG CGAAAGTATA TTGATTAGTT TTGGAGTACT CGcttcatgc
     2341 tgcaacgaac ggaggttaca ccaacaccag gaaggagatc tatgaagacg gcggcatctt
     2401 ggaggtcaac ttccgttaca cttacgagtt caacaagatc atcggtgacg tcgagtgcat
     2461 tggacatgga ttcccaagtc agagtccgat cttcaaggac acgatcgtga agagttgtcc
     2521 cacggtggac ctgatgttgc caatgtccgg gaacatcatc gccagctcct acgcttacgc
     2581 cttccaactg aaggacggct ctttctacac ggcagaagtc aagaacaaca tagacttcaa
     2641 gaatccaatc cacgagtcct tctcgaagtc agggcccatg ttcacccaca gacgtgtcga
     2701 ggagactctc accaaggaga accttgccat agtggagtac cagcaggttt tcaacagcgc
     2761 cccaagagac atgtagCGCG GTTCTATCTA GTTACGCGTT AAACCAACTA GAAATATGAA
     2821 GATGAAGATG AAATATTTGG TGTGTCAAAT AAAAAGCTTG TGTGCTTAAG TTTGTGTTTT
     2881 TTTCTTGGCT TGTTGTGTTA TGAATTTGTG GCTTTTTCTA ATATTAAATG AATGTAAGAT
     2941 CTCATTATAA TGAATAAACA AATGTTTCTA TAATCCATTG TGAATGTTTT GTTGGATCTC
     3001 TTCTGCAGCA TATAACTACT GTATGTGCTA TGGTATGGAC TATGGAATAT GATTAAAGAT
     3061 AAGATGGGCT CATAGAGTAA AACGAGGCGA GGGACCTATA AACCTCCCTT CATCATGCTA
     3121 TTTCATGATC TATTTTATAA AATAAAGATG TAGAAAAAAG TAAGCGTAAT AACCGCAAAA
     3181 CAAATGATTT AAAACATGGC ACATAATGAG GAGATTAAGT TCGGTTTACG TTTATTTTAG
     3241 TACTAATTGT AACGTGAGAC TACGTATCGG GAATCGCCTA ATTAAAGCAT TAATGCGAAC
     3301 CTGATTAGAT TCACCGACCC TCCTATCGTG TCGACCTTTC TGTTTCTTAG AATTTTTTGG
     3361 TAGTCTATGT ACTAATAATG TCAGCTTCGT ATTTATTTCA TAAGCAATTT GCATTTGCAA
     3421 TTTGTTTTTT ACTTTTATTT TTATTGTATT GTGGAATGTG GACTCGTACC AACATGAAGT
     3481 TATATACCAC CAAAAAAATT ACAGTTAGTC AAAAGATTCA CGAGTGAGAG CTACTTATGA
     3541 TTGTCTTTTA CGTATATGTC TAATTGTCTA TTTGCTCAAT AATCTTTGTA CTTTCTTTTG
     3601 TCGTTGATAA AATCACAAAG TTCCAAAAGT AATCGAATGA TTTGCTTTTA AGAAAAGAAG
     3661 AGCTCAATAA TTCAACATAT ATCTGTACAC Aattaccgcc tttgagtgag ctctagatgc
     3721 cacctgacgt ctaagaagaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3781 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3841 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3901 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3961 cgtattggct agagcagctt gccaacatgg tggagcacga cactctcgtc tactccaaga
     4021 atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
     4081 tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag
     4141 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc
     4201 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     4261 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg
     4321 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg
     4381 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
     4441 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc
     4501 attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg
     4561 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     4621 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
     4681 cgcaagacct tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca
     4741 ccagtctctc tctacaaatc tatctctctc gagctttcgc agatcccggg gggcaatgag
     4801 atatggggat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4861 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4921 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     4981 aactccagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     5041 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     5101 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     5161 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5221 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5281 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     5341 ccgacggcga ggatctcgtc gtgacacatg gcgatgcctg cttgccgaat atcatggtgg
     5401 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5461 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5521 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5581 ttcttgacga gttcttctga agtagatgcc gaccggatct gtcgatcgac aagctcgagt
     5641 ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc tatagggttt
     5701 cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc
     5761 tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca gatcccccga
     5821 attaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     5881 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     5941 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     6001 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     6061 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
     6121 tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg
     6181 atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa
     6241 ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg
     6301 gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc
     6361 accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc
     6421 tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga
     6481 cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct
     6541 ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa
     6601 tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag
     6661 ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat
     6721 atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg attacttact
     6781 gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga
     6841 tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc
     6901 ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat
     6961 tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc
     7021 gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat
     7081 caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct
     7141 agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7201 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7261 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7321 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     7381 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7441 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7501 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7561 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7621 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7681 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7741 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7801 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7861 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7921 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7981 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     8041 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     8101 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     8161 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     8221 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt
     8281 gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag
     8341 attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga
     8401 agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc
     8461 tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt
     8521 taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg
     8581 tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta
     8641 cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc
     8701 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc
     8761 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc
     8821 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa
     8881 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc
     8941 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc
     9001 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc
     9061 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt
     9121 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt
     9181 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc
     9241 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc
     9301 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat
     9361 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat
     9421 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc
     9481 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact
     9541 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt
     9601 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat
     9661 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg
     9721 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat
     9781 ttgtaccggg ccggatggtt tgcgaccgtc acgccgattc ctcgggcttg ggggttccag
     9841 tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc
     9901 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc
     9961 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc
    10021 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc
    10081 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct
    10141 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc
    10201 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa
    10261 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa
    10321 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa
    10381 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt
    10441 tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct
    10501 gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg
    10561 gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg
    10621 ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg
    10681 ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg
    10741 cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc
    10801 gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga
    10861 taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc
    10921 gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc
    10981 ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc
    11041 caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc
    11101 cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg
    11161 gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc
    11221 tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc
    11281 tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc
    11341 tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg
    11401 gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc
    11461 cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac
    11521 accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc
    11581 gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg
    11641 cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc
    11701 agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg
    11761 gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca
    11821 tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg
    11881 caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg
    11941 gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc
    12001 actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga
    12061 tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt
    12121 cacgcccttt taaatatccg ttattctaat aaacgctctt ttctcttagg tttacccgcc
    12181 aatatatcct gtcaaacact gatagtttaa ttcccgatct agtaacatag atgacaccgc
    12241 gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta
    12301 taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt
    12361 aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc
    12421 aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatcgg ggaaattcga
    12481 gctggtcacc aattcacacg tgatggtgat ggtgatggct agcgttcttg tagccgaaat
    12541 ctggaatgtt ggtccagcgc tcgcgaaaga cgtgcgcggc gagcttcggc ttgcggtcac
    12601 gagtgaacac gcccttcttg tttccttgga cgcgcatcac gccctgagag gtcgcgaagt
    12661 ccgcgaagtt ccacgcttgc tcacccacga agttctcaaa ctcatcgaac acgacgtggt
    12721 tcgcctggta gtactcgact tgatattcct cggtgaacat cactggatca atgtcgtgaa
    12781 agcccgcaac ggtgtctgcg ccgtactcag tgatcatgat cggctttcct gggcaacgct
    12841 tgttccacgc gtgaaattcc tggcggagat ggactttggc cgcttcgaga tcaccgccat
    12901 cgaagtacca tccgttatag cgattgagcg cgatgacgtc aatcagttcg gcgactttgt
    12961 ccgtctccgg ggtagccatc acaaacagca cgatcgtgac cggacgcttc tgtgggtcga
    13021 gttccttggt cagctccacc aacggcttga agtactcgta cgcgccctct tcctcagtcg
    13081 ccgcctcgtt ggcgatgctc cacatcacga cgcttggatg gttcttgtca cgagacacca
    13141 gttcacggag aacgtcttga tggtgctcaa acgtccgaat cttctcccag gtactgacgc
    13201 gctcgctgcc ttcgccgagt cccgtggtgg ccatgaagtt gaggtgcacg ccaactgccg
    13261 gagtctcgtc gatcacgacc agaccctcgc gatccgcaag acgcatcaac tcttcagagt
    13321 acggatagtg tgcggtccgg aagctgttgg cgccgatcca tttgaggata ttgaaatcca
    13381 tcacattgct cgcttcgtta aagccacggc cgttgatagg agtgtcctca tgtttgccaa
    13441 agcccttgaa gtagaacggt ttgttgttga tgaggaactt gccgtcgttg acttccacgg
    13501 tccgcacgcc gaacggctct tcatagacat cgatggtcag tccgtcgttc accagttcca
    13561 ctttgatctg gtagagatac gtgttcagtg gttcccagag gatgacattc ggaatctcca
    13621 cgttaccgct caggccctcg gtgcttgcga ccactttgcc ttcctcatcc acgaccgaca
    13681 ctttcacggt ctcggctttg ccttgaaagt ccaccgtata ggtcacagtc ccggttgggc
    13741 cattgaagtc ggtcacaacc gagatgtcct cgacgtacgt aaacggggtc gtgtagattt
    13801 tcaccggacg gtgcaggcct gcatagttga agaagtcgaa gttcggcttg ttacgaatga
    13861 cttttccgag gccctcttcg tggcgctcgc tgtacagccc caccgggagg gtgctatcgt
    13921 cgaggatgtt gtccacggcg acggtgacgc gattcatgcc atcacgcagc gagttgttga
    13981 tttccgcttc gaatggcagg aatccgccct tgtgctccac gaccagctca ccattgacat
    14041 agacaattgc tttgtgagtt gcagagccga agcggagcac gatacgctga tccttcagat
    14101 aggccggcac cgtgaactca cgttcgtacc agacatatcc gatatggttg cggatttcct
    14161 tggtcacgcc aatgtcattg taactgcttg ggacggccat actaatagtg tcggtcagct
    14221 tgctttcgta ccacttctct tccagtcctt tcccgtagtc cagcttgaag ttccagacgc
    14281 cattgaggtc gaagacgcca cgggtctcgg tgttgatcgg gtacagacta gttcgtcggt
    14341 tctgtaacta tcatcatcat catagacaca cgaaataaag taatcagatt atcagttaaa
    14401 gctatgtaat atttacacca taaccaatca attaaaaaat agatcagttt aaagaaagat
    14461 caaagctcaa aaaaataaaa agagaaaagg gtcctaacca agaaaatgaa ggagaaaaac
    14521 tagaaattta ccctcagatc taccatg
//