LOCUS       pXYB108_35S_UTR_GFPuv_ 14136 bp ds-DNA     circular     25-SEP-2023
DEFINITION  .
KEYWORDS    "creator:Xiaohan Yang" "marker:NeoR/KanR, KanR, Modified
            COR47-5'-UTR"
COMMENT     pGFPuv difested by PacI and SbfI + gYL091
FEATURES             Location/Qualifiers
     promoter        complement(16..361)
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     primer_bind     738..754
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     misc_feature    745..774
                     /label="gibson overhang"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    745..1756
                     /label="gyl091"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     promoter        1269..1614
                     /label="CaMV 35S promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter from cauliflower mosaic virus"
     misc_feature    1599..1614
                     /label="overlap1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1615..1721
                     /label="Modified COR47-5'-UTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1697..1726
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1697..2949
                     /label="gYL095"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1727..1756
                     /label="GIBSON OVERHANG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1730..1754
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             1730..2389
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MTTFKIESRIHGNLNGEKFELVGGGVGEEGRLEIEMKTKDKPLAFSPFLLTTCMGYGFYHFASFPKGIKNIYLHAATNGGYTNTRKEIYEDGGILEVNFRYTYEFNKIIGDVECIGHGFPSQSPIFKDTIVKSCPTVDLMLPMSGNIIASSYAYAFQLKDGSFYTAEVKNNIDFKNPIHESFSKSGPMFTHRRVEETLTKENLAIVEYQQVFNSAPRDM*"
     misc_feature    1730..2389
                     /label="eYGFPuv"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1760..1784
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2290..2314
                     /label="overlap"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2390..2402
                     /label="gq2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2403..3280
                     /label="HSPT878"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2428..2452
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2914..2949
                     /label="gibson overhang"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3281..3301
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3332..3356
                     /label="overlap"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(3342..3358)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    3366..3382
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3390..3396)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3397..3414)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(3415..3420)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    3435..3456
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        3647..4324
                     /label="CaMV 35S promoter (enhanced)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: cauliflower mosaic virus 35S promoter with a duplicated enhancer region"
     misc_feature    4328..5254
                     /label="gBlocks for change to Kan"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4392..5189
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-II (or nptII) note: confers resistance to neomycin, kanamycin, and G418 (Geneticin®) product: aminoglycoside phosphotransferase from Tn5 transl_table: 1 translation: MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     polyA_signal    5229..5403
                     /label="CaMV poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: cauliflower mosaic virus polyadenylation signal"
     misc_feature    5481..5505
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: left border repeat from nopaline C58 T-DNA"
     CDS             5930..6724
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aphA-3 note: confers resistance to kanamycin product: aminoglycoside phosphotransferase protein_id: transl_table: 1 translation: MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"
     rep_origin      6811..7399
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     misc_feature    7585..7725
                     /label="bom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: basis of mobility region from pBR322"
     rep_origin      8069..8263
                     /label="pVS1 oriV"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)"
     CDS             complement(8329..9396)
                     /label="pVS1 RepA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 note: product: replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) protein_id: transl_table: 1 translation: GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     CDS             complement(9830..10459)
                     /label="pVS1 StaA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 product: stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) transl_table: 1 translation: MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
     misc_feature    11759..11783
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: right border repeat from nopaline C58 T-DNA"
     terminator      11805..12057
                     /label="NOS terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: nopaline synthase terminator and poly(A) signal"
     CDS             complement(12089..12106)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: 6xHis affinity tag transl_table: 1 translation: HHHHHH"
     CDS             complement(12113..13930)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          13931..14120
                     /label="cat1 intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: castor bean catalase intron, modified"
     CDS             complement(13931..14120)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     CDS             complement(14121..14135)
                     /label="GUSPlus™"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: note: codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria product: β-glucuronidase protein_id: transl_table: 1 translation: MVDLR,,NRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacaCAAACA
     1621 TTACTCATTC ACAAAACCAT CTTAAAGCAA CTACACAAGT CTTGAAATTT TCTCATATTT
     1681 TCTATTTACT ATATAAACTT TTAATCAAAT CAAGATTAAA GTTAATTAAa tgacaacctt
     1741 caaaatcgag tcccggatcc acggcaacct caacggggag aagttcgagt tggttggagg
     1801 tggagtaggt gaggagggtc gcctcgagat tgagatgaag actaaagata aaccactggc
     1861 attctctccc ttcctgctga ccacttgcat gggttacggg ttctaccact tcgccagctt
     1921 cccaaagggg attaagaaca tctatcttca tgctgcaacg aacggaggtt acaccaacac
     1981 caggaaggag atctatgaag acggcggcat cttggaggtc aacttccgtt acacttacga
     2041 gttcaacaag atcatcggtg acgtcgagtg cattggacat ggattcccaa gtcagagtcc
     2101 gatcttcaag gacacgatcg tgaagagttg tcccacggtg gacctgatgt tgccaatgtc
     2161 cgggaacatc atcgccagct cctacgctta cgccttccaa ctgaaggacg gctctttcta
     2221 cacggcagaa gtcaagaaca acatagactt caagaatcca atccacgagt ccttctcgaa
     2281 gtcagggccc atgttcaccc acagacgtgt cgaggagact ctcaccaagg agaaccttgc
     2341 catagtggag taccagcagg ttttcaacag cgccccaaga gacatgtagT GGTGGTGGTG
     2401 GTATATGAAG ATGAAGATGA AATATTTGGT GTGTCAAATA AAAAGCTTGT GTGCTTAAGT
     2461 TTGTGTTTTT TTCTTGGCTT GTTGTGTTAT GAATTTGTGG CTTTTTCTAA TATTAAATGA
     2521 ATGTAAGATC TCATTATAAT GAATAAACAA ATGTTTCTAT AATCCATTGT GAATGTTTTG
     2581 TTGGATCTCT TCTGCAGCAT ATAACTACTG TATGTGCTAT GGTATGGACT ATGGAATATG
     2641 ATTAAAGATA AGATGGGCTC ATAGAGTAAA ACGAGGCGAG GGACCTATAA ACCTCCCTTC
     2701 ATCATGCTAT TTCATGATCT ATTTTATAAA ATAAAGATGT AGAAAAAAGT AAGCGTAATA
     2761 ACCGCAAAAC AAATGATTTA AAACATGGCA CATAATGAGG AGATTAAGTT CGGTTTACGT
     2821 TTATTTTAGT ACTAATTGTA ACGTGAGACT ACGTATCGGG AATCGCCTAA TTAAAGCATT
     2881 AATGCGAACC TGATTAGATT CACCGACCCT CCTATCGTGT CGACCTTTCT GTTTCTTAGA
     2941 ATTTTTTGGT AGTCTATGTA CTAATAATGT CAGCTTCGTA TTTATTTCAT AAGCAATTTG
     3001 CATTTGCAAT TTGTTTTTTA CTTTTATTTT TATTGTATTG TGGAATGTGG ACTCGTACCA
     3061 ACATGAAGTT ATATACCACC AAAAAAATTA CAGTTAGTCA AAAGATTCAC GAGTGAGAGC
     3121 TACTTATGAT TGTCTTTTAC GTATATGTCT AATTGTCTAT TTGCTCAATA ATCTTTGTAC
     3181 TTTCTTTTGT CGTTGATAAA ATCACAAAGT TCCAAAAGTA ATCGAATGAT TTGCTTTTAA
     3241 GAAAAGAAGA GCTCAATAAT TCAACATATA TCTGTACACA attaccgcct ttgagtgagc
     3301 tctagatgcc acctgacgtc taagaagaat tcgtaatcat ggtcatagct gtttcctgtg
     3361 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     3421 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     3481 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     3541 ggcggtttgc gtattggcta gagcagcttg ccaacatggt ggagcacgac actctcgtct
     3601 actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     3661 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca
     3721 aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg
     3781 ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     3841 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
     3901 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     3961 accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
     4021 attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca
     4081 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc
     4141 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     4201 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     4261 actatccttc gcaagacctt cctctatata aggaagttca tttcatttgg agaggacacg
     4321 ctgaaatcac cagtctctct ctacaaatct atctctctcg agctttcgca gatcccgggg
     4381 ggcaatgaga tatggggatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4441 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4501 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4561 ccctgaatga actccaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4621 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4681 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4741 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4801 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4861 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4921 cgcgcatgcc cgacggcgag gatctcgtcg tgacacatgg cgatgcctgc ttgccgaata
     4981 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5041 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5101 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5161 tctatcgcct tcttgacgag ttcttctgaa gtagatgccg accggatctg tcgatcgaca
     5221 agctcgagtt tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct
     5281 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
     5341 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag
     5401 atcccccgaa ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
     5461 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
     5521 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
     5581 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
     5641 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
     5701 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg
     5761 gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct
     5821 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag
     5881 cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat
     5941 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
     6001 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
     6061 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
     6121 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
     6181 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
     6241 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
     6301 catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga
     6361 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc
     6421 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
     6481 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
     6541 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
     6601 cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
     6661 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
     6721 ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6781 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6841 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6901 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6961 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7021 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7081 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7141 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7201 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7261 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7321 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7381 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7441 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7501 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7561 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     7621 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     7681 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     7741 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     7801 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     7861 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     7921 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
     7981 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     8041 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     8101 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     8161 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     8221 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg
     8281 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     8341 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     8401 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
     8461 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
     8521 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
     8581 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
     8641 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
     8701 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
     8761 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
     8821 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
     8881 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
     8941 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
     9001 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
     9061 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
     9121 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
     9181 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
     9241 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
     9301 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
     9361 cgcgccgatt tgtaccgggc cggatggttt gcgaccgtca cgccgattcc tcgggcttgg
     9421 gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc
     9481 ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca
     9541 tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct catttactct
     9601 ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg
     9661 tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct
     9721 ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg
     9781 gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt
     9841 ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg
     9901 attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac
     9961 gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc
    10021 gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct
    10081 caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg
    10141 gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg
    10201 cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt
    10261 caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc
    10321 aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt
    10381 gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa
    10441 gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat
    10501 atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg
    10561 gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga
    10621 caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac
    10681 gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg
    10741 gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg
    10801 gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc
    10861 gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt
    10921 gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc
    10981 tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg
    11041 cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac
    11101 acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta
    11161 cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg
    11221 cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc
    11281 atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg
    11341 gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg
    11401 acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg
    11461 ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa
    11521 aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa
    11581 gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa
    11641 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
    11701 aaaccttttc acgccctttt aaatatccgt tattctaata aacgctcttt tctcttaggt
    11761 ttacccgcca atatatcctg tcaaacactg atagtttaat tcccgatcta gtaacataga
    11821 tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta
    11881 ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca
    11941 ttacatgtta attattacat gcttaacgta attcaacaga aattatatga taatcatcgc
    12001 aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg
    12061 gaaattcgag ctggtcacca attcacacgt gatggtgatg gtgatggcta gcgttcttgt
    12121 agccgaaatc tggaatgttg gtccagcgct cgcgaaagac gtgcgcggcg agcttcggct
    12181 tgcggtcacg agtgaacacg cccttcttgt ttccttggac gcgcatcacg ccctgagagg
    12241 tcgcgaagtc cgcgaagttc cacgcttgct cacccacgaa gttctcaaac tcatcgaaca
    12301 cgacgtggtt cgcctggtag tactcgactt gatattcctc ggtgaacatc actggatcaa
    12361 tgtcgtgaaa gcccgcaacg gtgtctgcgc cgtactcagt gatcatgatc ggctttcctg
    12421 ggcaacgctt gttccacgcg tgaaattcct ggcggagatg gactttggcc gcttcgagat
    12481 caccgccatc gaagtaccat ccgttatagc gattgagcgc gatgacgtca atcagttcgg
    12541 cgactttgtc cgtctccggg gtagccatca caaacagcac gatcgtgacc ggacgcttct
    12601 gtgggtcgag ttccttggtc agctccacca acggcttgaa gtactcgtac gcgccctctt
    12661 cctcagtcgc cgcctcgttg gcgatgctcc acatcacgac gcttggatgg ttcttgtcac
    12721 gagacaccag ttcacggaga acgtcttgat ggtgctcaaa cgtccgaatc ttctcccagg
    12781 tactgacgcg ctcgctgcct tcgccgagtc ccgtggtggc catgaagttg aggtgcacgc
    12841 caactgccgg agtctcgtcg atcacgacca gaccctcgcg atccgcaaga cgcatcaact
    12901 cttcagagta cggatagtgt gcggtccgga agctgttggc gccgatccat ttgaggatat
    12961 tgaaatccat cacattgctc gcttcgttaa agccacggcc gttgatagga gtgtcctcat
    13021 gtttgccaaa gcccttgaag tagaacggtt tgttgttgat gaggaacttg ccgtcgttga
    13081 cttccacggt ccgcacgccg aacggctctt catagacatc gatggtcagt ccgtcgttca
    13141 ccagttccac tttgatctgg tagagatacg tgttcagtgg ttcccagagg atgacattcg
    13201 gaatctccac gttaccgctc aggccctcgg tgcttgcgac cactttgcct tcctcatcca
    13261 cgaccgacac tttcacggtc tcggctttgc cttgaaagtc caccgtatag gtcacagtcc
    13321 cggttgggcc attgaagtcg gtcacaaccg agatgtcctc gacgtacgta aacggggtcg
    13381 tgtagatttt caccggacgg tgcaggcctg catagttgaa gaagtcgaag ttcggcttgt
    13441 tacgaatgac ttttccgagg ccctcttcgt ggcgctcgct gtacagcccc accgggaggg
    13501 tgctatcgtc gaggatgttg tccacggcga cggtgacgcg attcatgcca tcacgcagcg
    13561 agttgttgat ttccgcttcg aatggcagga atccgccctt gtgctccacg accagctcac
    13621 cattgacata gacaattgct ttgtgagttg cagagccgaa gcggagcacg atacgctgat
    13681 ccttcagata ggccggcacc gtgaactcac gttcgtacca gacatatccg atatggttgc
    13741 ggatttcctt ggtcacgcca atgtcattgt aactgcttgg gacggccata ctaatagtgt
    13801 cggtcagctt gctttcgtac cacttctctt ccagtccttt cccgtagtcc agcttgaagt
    13861 tccagacgcc attgaggtcg aagacgccac gggtctcggt gttgatcggg tacagactag
    13921 ttcgtcggtt ctgtaactat catcatcatc atagacacac gaaataaagt aatcagatta
    13981 tcagttaaag ctatgtaata tttacaccat aaccaatcaa ttaaaaaata gatcagttta
    14041 aagaaagatc aaagctcaaa aaaataaaaa gagaaaaggg tcctaaccaa gaaaatgaag
    14101 gagaaaaact agaaatttac cctcagatct accatg
//